The document describes Invetech's NovoGen MMX Bioprinter, which is the world's first commercial 3D bioprinter. It can print layers of cell aggregates and biogel to construct tissues and blood vessels. The bioprinter has high precision motion axes and dual print heads to print both biogel and cells. It also has thermal control of the biogel and automated cartridge loading. The bioprinter reduces the time needed for blood vessel printing from over 8 hours previously to less than an hour. It provides precise printing down to 20 microns and uses laser calibration and closed-loop motor control. Examples are given of tissues and applications printed so far, ranging from liver tissue to cartilage

1. The World’s First
Commercial 3D
Bioprinter
Chris Leigh-Lancaster

2. Introducing Invetech
A synergistic mix of biomedical, automation, high
precision, fluid handling and user experience ...
All the right ingredients for bioprinting!
More than 30 years of product realization
Page 2
Diagnostics Cell TherapyPoint of Care
Consumer
& Industrial

3. Introducing Invetech
A diverse project mix
Page 3

4. Making Human Tissue
The community need – Organ/tissue replacement
Page 4

5. Making Human Tissue
The community need – Tissue for drug discovery
Page 5

6. Printing Human Tissue
Science fiction becomes reality
Page 6

7. Making Human Tissue
Step 1 – Cells are harvested aggregates and loaded into cartridges
Page 7

8. Page 8
Making Human Tissue
Step 2 – The Bioprinter prints layers of Cell aggregates and BioGel
Extruded BioGel
lines
Cartridge with BioGel
or cell aggregates
3D print
nozzle

9. Page 9
Making Human Tissue
Step 3 – Construct is placed into a bioreactor and incubated

10. The NovoGen MMX Bioprinter™
Key features
Page 10
• 3 high-precision motion
axes
• Dual print heads for
BioGel and cell layers
• Thermal control of the
BioGel
• Semi-automated loading
of print cartridges
• An intuitive GUI for rapid
vessel/tissue design

11. The NovoGen MMX Bioprinter™
Initial blood vessel printing on the Bioprinter
Page 11
500 μm glass
cartridge
dispense tip
BioGel structure
containing BioGel and
cell lines
Blood vessel
geometry within the
BioGel structure

12. Simple Operation
Intuitive graphical user interface (GUI)
Page 12
 WPF-based flexible GUI - customisable for future applications
 Key focus is easy translation of simple tissue designs into real tissue
 “mine sweeper” style designer for developing 2D geometric forms
 Built-in scripting language for design of more complex 3D constructs

13. Simple Operation
Cartridge loading & alignment
Page 13
Before the Bioprinter …
 Blood vessel printing took 8 hours or more
With the Bioprinter – less than an hour
 Cartridge loading & alignment took up to 45 mins
With the Bioprinter - less than 1 minute
Thanks to ...
 Intuitive cartridge loading with precision
alignment
 Automatic positive displacement dispense
 Automatic laser calibration of cartridge tip
position

14. Precision Printing
What’s needed for successful printing?
Page 14
 Positional precision of better than ± 20 microns
 Re-calibration of tip positions for each cartridge
 Smooth dispense of cells and BioGel
 Bio-compatible operation
100 µm human hair

15. Precision Printing
How it’s done …
Page 15
 Optical-grade robotic axes with single micron precision
 Novel 3D laser tip calibration using custom interpolation algorithms
 Closed loop micro-stepping motor control to minimize vibration
 Designed for the bio-safety cabinet

16. From Science to Application
A timeline from the first blood vessel
Page 16
December 2010
First human blood vessel
Time Magazine’s Best Inventions of 2010
Mar 2011
Partnerships with big pharma
Nov 2011
Australian Engineering Innovation Award.
Dec 2012
Autodesk partnership
Feb 2013
Refined Bioprinters delivered to Organovo

17. From Science to Application
A timeline from the first blood vessel
Page 17
Apr 2013
First 3D liver tissue. Life span increases from 5 to 40 days
Apr 2014
Contracting for 3D liver tissue toxicity testing
The Near Future
3D tissue constructs replace existing pre-clinical trial methods for drug screening.
Simple tissues start being used for direct surgical therapy

18. Alternate Blood Vessel Manufacturing
Page 18
“Off the shelf” human veins for use in life-saving vascular surgery
Humacyte Artificial Vascular Graft (HAVGTM)

19. Regenerative Medicine
SCORE center at Scripps Clinic- Cartilage printing
Page 19

20. Consumer Products
Modern Meadow - Cow-less leather and meat
Page 20

21. Cell Therapies
Innovacell - ICES 13 and ICEF 15
Page 21
Argos Therapeutics - ArcelisTM
Treating incontinence and cancer

22. Page 22Invetech confindential file: A3_3D Bioprinter - Inside 3D Printing Conference.pptx
www.invetech.us
www.linkedin.com/company/invetech
https://twitter.com/Invetech
Photo: Timothy Hogan