Glycolysis is a central pathway for glucose catabolism that converts glucose into pyruvate through a series of 10 enzyme-catalyzed reactions. It occurs in most organisms and tissues as a source of energy. The first phase activates glucose through phosphorylation, while the second phase generates ATP and NADH through substrate-level phosphorylation and hydride transfer. Pyruvate produced can then undergo aerobic or anaerobic fates including fermentation to regenerate NAD+ under anaerobic conditions.
Glycolysis is the breakdown of glucose to pyruvate through a series of enzyme-catalyzed reactions. It occurs in the cytosol and consists of a preparatory phase requiring ATP and a payoff phase generating ATP. Key steps include phosphorylation by hexokinase, aldolase cleavage, substrate-level phosphorylation by phosphoglycerate kinase, and pyruvate formation by pyruvate kinase. Glycolytic enzymes are regulated by feedback inhibition and metabolites like fructose 2,6-bisphosphate and AMP/ATP ratios to control flux through the pathway.
The document discusses various modes of regulating enzyme activity, including allosteric regulation, covalent modification, induction and repression, compartmentalization, and isoenzymes. Allosteric regulation involves effector molecules binding at allosteric sites and inducing conformational changes that increase or decrease the enzyme's activity. Covalent modification can activate or inactivate enzymes through additions like phosphorylation. Induction and repression alter the amount of enzyme by increasing or decreasing its synthesis in response to signals. Compartmentalization separates pathways to increase efficiency, while isoenzymes form multienzyme complexes for the same purpose.
BIOSYNTHESIS OF PHOSPHOLIPIDS
Phospholipids:-
These are compounds containing, in addition to fatty acid and glycerol, phosphoric acid, nitrogenous bases, and another substituent. Polar compounds composed of alcohol attached by phosphodiester bridge to either diacylglycerol or sphingosine.
Amphipathic in nature has a hydrophilic head (phosphate +alcohol
eg., serine, ethanolamine, and choline) and a long, hydrophobic tail
(fatty acids or derivatives ).
- CLASSIFICATION OF PHOSPHOLIPIDS:-
- Glycerophospholipids
- Spingophospholipids or Sphingomyelin
- SYNTHESIS OF PHOSPHOLIPIDS
- FUNCTIONS OF PHOSPHOLIPIDS
- FUNCTIONS OF SPHINGOLIPIDS
Biosynthesis of fatty acids occurs predominantly in the liver, kidney, adipose tissue and mammary glands. Acetyl CoA and NADPH provide the building blocks and reducing power for fatty acid formation. Fatty acid synthesis occurs in three stages: 1) Acetyl CoA and NADPH are produced in the mitochondria and cytosol, respectively; 2) Acetyl CoA is carboxylated to malonyl CoA by acetyl CoA carboxylase; 3) The fatty acid synthase complex catalyzes the reactions to elongate the fatty acid chain by two carbons at a time using malonyl CoA, producing a longer fatty acid with each cycle.
This document discusses glycolipids, which are lipids that contain one or more sugar molecules. Glycolipids are classified as glycosphingolipids, globosides, gangliosides, and sulfatides. Glycosphingolipids contain ceramide and one or more sugars. Gangliosides contain sialic acid and contribute to cell membrane structure and function. Genetic defects that prevent the breakdown of glycolipids cause lipid storage diseases like Gaucher's disease and Tay-Sachs disease, leading to lipid accumulation in tissues and associated symptoms. Laboratory tests can diagnose these conditions by measuring enzyme levels or examining tissues. Some lipid storage diseases can be treated through enzyme replacement therapy.
The document discusses oxidation-reduction (redox) reactions in biological systems. It begins by defining oxidation as the removal of electrons and reduction as the gain of electrons. It states that redox reactions involve the transfer of electrons from substances of higher electrochemical potential to those of lower potential. The document outlines several types of redox enzymes, including oxidases, dehydrogenases, hydroperoxidases, and oxygenases. It provides examples of important redox reactions and enzymes in biological systems, such as cytochrome oxidase and alcohol dehydrogenase. The role of redox reactions in energy production through electron transport chains is also briefly mentioned.
High energy compounds, also known as energy rich compounds, contain high energy bonds that release free energy of -7.3 kcal/mol or greater during hydrolysis. ATP is the most important high energy compound, containing two high energy phosphoanhydride bonds. The hydrolysis of ATP releases -7.3 kcal/mol of free energy and is coupled to endergonic reactions in cells, functioning to link catabolic and anabolic processes through the transfer of its phosphoryl groups. ATP serves as the universal energy currency in living organisms, driving many biological reactions and processes.
This document discusses various mechanisms of enzyme regulation in living systems. It begins by explaining that hundreds of enzyme-catalyzed reactions must be precisely controlled for proper cellular functioning. It then describes several key mechanisms by which this regulation can be achieved, including allosteric regulation, isoenzyme expression, zymogen activation, and covalent modification via phosphorylation or glycosylation. Specific examples are provided for each type of regulation, such as feedback inhibition of threonine dehydratase and phosphorylation control of glycogen phosphorylase activity. The document concludes by emphasizing that multiple regulatory strategies acting together ensure survival of the cell and maintenance of homeostasis.
Glycolysis is the breakdown of glucose to pyruvate through a series of enzyme-catalyzed reactions. It occurs in the cytosol and consists of a preparatory phase requiring ATP and a payoff phase generating ATP. Key steps include phosphorylation by hexokinase, aldolase cleavage, substrate-level phosphorylation by phosphoglycerate kinase, and pyruvate formation by pyruvate kinase. Glycolytic enzymes are regulated by feedback inhibition and metabolites like fructose 2,6-bisphosphate and AMP/ATP ratios to control flux through the pathway.
The document discusses various modes of regulating enzyme activity, including allosteric regulation, covalent modification, induction and repression, compartmentalization, and isoenzymes. Allosteric regulation involves effector molecules binding at allosteric sites and inducing conformational changes that increase or decrease the enzyme's activity. Covalent modification can activate or inactivate enzymes through additions like phosphorylation. Induction and repression alter the amount of enzyme by increasing or decreasing its synthesis in response to signals. Compartmentalization separates pathways to increase efficiency, while isoenzymes form multienzyme complexes for the same purpose.
BIOSYNTHESIS OF PHOSPHOLIPIDS
Phospholipids:-
These are compounds containing, in addition to fatty acid and glycerol, phosphoric acid, nitrogenous bases, and another substituent. Polar compounds composed of alcohol attached by phosphodiester bridge to either diacylglycerol or sphingosine.
Amphipathic in nature has a hydrophilic head (phosphate +alcohol
eg., serine, ethanolamine, and choline) and a long, hydrophobic tail
(fatty acids or derivatives ).
- CLASSIFICATION OF PHOSPHOLIPIDS:-
- Glycerophospholipids
- Spingophospholipids or Sphingomyelin
- SYNTHESIS OF PHOSPHOLIPIDS
- FUNCTIONS OF PHOSPHOLIPIDS
- FUNCTIONS OF SPHINGOLIPIDS
Biosynthesis of fatty acids occurs predominantly in the liver, kidney, adipose tissue and mammary glands. Acetyl CoA and NADPH provide the building blocks and reducing power for fatty acid formation. Fatty acid synthesis occurs in three stages: 1) Acetyl CoA and NADPH are produced in the mitochondria and cytosol, respectively; 2) Acetyl CoA is carboxylated to malonyl CoA by acetyl CoA carboxylase; 3) The fatty acid synthase complex catalyzes the reactions to elongate the fatty acid chain by two carbons at a time using malonyl CoA, producing a longer fatty acid with each cycle.
This document discusses glycolipids, which are lipids that contain one or more sugar molecules. Glycolipids are classified as glycosphingolipids, globosides, gangliosides, and sulfatides. Glycosphingolipids contain ceramide and one or more sugars. Gangliosides contain sialic acid and contribute to cell membrane structure and function. Genetic defects that prevent the breakdown of glycolipids cause lipid storage diseases like Gaucher's disease and Tay-Sachs disease, leading to lipid accumulation in tissues and associated symptoms. Laboratory tests can diagnose these conditions by measuring enzyme levels or examining tissues. Some lipid storage diseases can be treated through enzyme replacement therapy.
The document discusses oxidation-reduction (redox) reactions in biological systems. It begins by defining oxidation as the removal of electrons and reduction as the gain of electrons. It states that redox reactions involve the transfer of electrons from substances of higher electrochemical potential to those of lower potential. The document outlines several types of redox enzymes, including oxidases, dehydrogenases, hydroperoxidases, and oxygenases. It provides examples of important redox reactions and enzymes in biological systems, such as cytochrome oxidase and alcohol dehydrogenase. The role of redox reactions in energy production through electron transport chains is also briefly mentioned.
High energy compounds, also known as energy rich compounds, contain high energy bonds that release free energy of -7.3 kcal/mol or greater during hydrolysis. ATP is the most important high energy compound, containing two high energy phosphoanhydride bonds. The hydrolysis of ATP releases -7.3 kcal/mol of free energy and is coupled to endergonic reactions in cells, functioning to link catabolic and anabolic processes through the transfer of its phosphoryl groups. ATP serves as the universal energy currency in living organisms, driving many biological reactions and processes.
This document discusses various mechanisms of enzyme regulation in living systems. It begins by explaining that hundreds of enzyme-catalyzed reactions must be precisely controlled for proper cellular functioning. It then describes several key mechanisms by which this regulation can be achieved, including allosteric regulation, isoenzyme expression, zymogen activation, and covalent modification via phosphorylation or glycosylation. Specific examples are provided for each type of regulation, such as feedback inhibition of threonine dehydratase and phosphorylation control of glycogen phosphorylase activity. The document concludes by emphasizing that multiple regulatory strategies acting together ensure survival of the cell and maintenance of homeostasis.
This document discusses fatty acid biosynthesis. It begins with an introduction to fatty acids, their types and functions. It then describes the localization and key steps of biosynthesis, which involves condensation, reduction, dehydration and reduction reactions. The main enzymes involved are acetyl-CoA carboxylase and fatty acid synthase. Biosynthesis occurs via fatty acid synthase and produces palmitate in 7 cycles. It is regulated differently in plants versus animals. Fatty acids have important structural and biological functions in both plants and animals.
Glycoproteins are proteins that contain carbohydrate chains covalently attached. They can be O-linked, N-linked or GPI-anchored. Glycoproteins play important structural and functional roles like cell adhesion and acting as receptors. They are synthesized through a complex process in the endoplasmic reticulum and Golgi apparatus. Congenital disorders of glycosylation can occur from mutations affecting glycoprotein synthesis. Blood groups are also determined by glycoproteins on red blood cell surfaces.
Gluconeogenesis is the process by which glucose is synthesized from non-carbohydrate precursors in the liver and kidneys. It occurs mainly during periods of fasting and involves converting substrates like lactate, glycerol, and certain amino acids into glucose. The pathway overcomes three thermodynamic barriers of glycolysis through smaller successive steps. Regulation occurs through allosteric control of enzymes, hormonal control of fructose 2,6-bisphosphate levels, and transcriptional control of key genes like PEPCK and FOXO1. Together these mechanisms help direct carbon fluxes towards gluconeogenesis or glycolysis based on energy demands.
Glycogen is stored in the liver and muscles as a fuel reserve that can be easily mobilized to generate energy in the absence of oxygen. Glycogenesis is the process of glycogen synthesis from glucose, requiring enzymes like glycogen synthase. Glycogenolysis is the degradation of stored glycogen into glucose-6-phosphate and glucose by glycogen phosphorylase. The activities of glycogen synthase and phosphorylase are regulated by allosteric effectors and hormones like glucagon, epinephrine, and insulin to balance glycogen synthesis and breakdown. Genetic defects in glycogen metabolism can cause glycogen storage diseases.
Jayati Mishra presented on the de novo and salvage pathways of purines under the guidance of Pradip Hirapue. The presentation discussed:
1) The de novo pathway synthesizes purine nucleotides from simple precursors through a two-stage process forming IMP and then converting it to AMP or GMP.
2) The salvage pathway recycles purine bases and nucleosides obtained from the diet or cell turnover to form nucleotides.
3) Both pathways work together to synthesize the purine nucleotides needed for nucleic acid synthesis, with the salvage pathway playing a larger role in certain tissues.
1) Organisms require chemical energy stored in high-energy compounds for processes like muscle contraction and active transport.
2) High-energy compounds include ATP, phosphoenolpyruvate, and acetyl-CoA, which contain high-energy bonds like phosphoanhydride and thioester bonds.
3) ATP is the most common energy currency in cells. It stores and transports chemical energy through its high-energy phosphoanhydride bonds, which are hydrolyzed to fuel energetic reactions.
The document discusses different types of phospholipids and their structures. It describes glycerophospholipids, which contain glycerol, fatty acids, phosphoric acid, and a nitrogenous base. Major glycerophospholipids mentioned are phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, phosphatidylinositol, cardiolipin, and plasmalogen. Sphingophospholipids contain sphingosine instead of glycerol. Sphingomyelin is provided as an example. Key functions and tissue locations of different phospholipids are also summarized.
Sphingolipids are a class of lipids that include sphingomyelin, glycosphingolipids, and ceramide. They serve important structural and signaling functions in the cell membrane and participate in processes like cell growth, differentiation, and apoptosis. Sphingolipids are synthesized through de novo pathways or through the breakdown of sphingomyelin. Their metabolites, including ceramide, sphingosine-1-phosphate, and gangliosides act as second messengers and influence pathways such as PI3K/Akt and JNK. Sphingolipids are also components of lipid rafts and caveolae, which regulate protein activity and cellular signaling. Imbalances in sph
Inhibitors & uncouplers of oxidative phosphorylation & ETCDipesh Tamrakar
The document provides an overview of oxidative phosphorylation and electron transport chain inhibitors and uncouplers. It discusses key concepts like the Q-cycle, shuttle systems that transport cytosolic NADH into mitochondria, uncoupling proteins, and various inhibitors that target different parts of the electron transport chain and oxidative phosphorylation. Specific inhibitors and uncouplers mentioned include rotenone, antimycin, oligomycin, 2,4-dinitrophenol, and chloro carbonyl cyanide phenyl hydrazone. Thyroid hormones are also noted to play a role in regulating uncoupling proteins and thermogenesis.
Oligomeric enzymes consist of two or more polypeptide chains linked together by non-covalent interactions. Examples of oligomeric enzymes include lactate dehydrogenase, which is a tetramer, and tryptophan synthase, which contains two different subunits that each have distinct catalytic functions. Oligomeric enzyme organization allows for complex regulation through allostery and feedback inhibition not possible with monomeric enzymes.
The pentose phosphate pathway, also known as the hexose monophosphate shunt, is an alternative metabolic pathway to glycolysis that occurs in the cytosol. It is a complex pathway that helps generate NADPH for fatty acid synthesis and glutathione for antioxidant activity, as well as synthesizing ribose-5-phosphate for nucleotide and nucleic acid formation. Glucose-6-phosphate enters the pathway and is oxidized through a two-phase process involving dehydrogenation using NADP+ instead of NAD+. Several intermediates are formed and rearranged before regenerating glucose-6-phosphate and glyceraldehyde-3-phosphate to complete the nonoxidative phase. Genetic defects in glucose-6
This document summarizes the metabolic pathways of gluconeogenesis and glycogenolysis. It explains that gluconeogenesis synthesizes glucose from non-carbohydrate precursors through a series of steps that are largely the reverse of glycolysis, with three bypass reactions. Glycogenolysis breaks down glycogen stores in the liver and muscle into glucose through cleavage of glucose monomers by glycogen phosphorylase and subsequent conversion to glucose-6-phosphate. Both pathways are regulated by hormones like glucagon and epinephrine.
Triacylglycerols are the main form in which animals store fat and are composed of glycerol bonded to three fatty acid chains. They are insoluble in water and primarily function as energy reserves, being stored in adipose tissue. Triacylglycerols can be either simple, containing the same fatty acid at each position, or mixed, containing different fatty acids. They undergo hydrolysis for digestion and energy release and can become rancid if exposed to air, moisture, or bacteria through oxidative or hydrolytic processes. Antioxidants help prevent rancidity.
This document discusses allosteric enzymes, which have additional binding sites called allosteric sites that are distinct from the active site. Molecules that bind to these allosteric sites, called effectors, can cause a conformational change in the enzyme's structure that increases or decreases its catalytic activity. There are two main models that describe the mechanism of allostery: the concerted model proposed by Monod, Wyman, and Changeux and the sequential model proposed by Koshland, Nemethy, and Filmer. Allosteric effectors can be positive or negative, and allosteric regulation can be homotropic, involving the substrate, or heterotropic, involving a different molecule. Allo
This document discusses the biosynthesis of phospholipids. It begins by defining phospholipids as complex lipids containing phosphoric acid, fatty acids, nitrogenous bases, and alcohols. Phospholipids are synthesized primarily on the surfaces of the smooth endoplasmic reticulum and transported via vesicles to their destinations. There are two main types of phospholipids - glycerophospholipids and sphingolipids. Glycerophospholipids have asymmetrical fatty acid groups attached to carbon 1 and 2 of the glycerol backbone. They are synthesized by attaching two fatty acyl groups to glycerol-3-phosphate to form phosphatidic acid. The document then discusses the synthesis of specific phospholipids
1) Allosteric enzymes have additional sites called allosteric sites that are distinct from the active site. Binding of an effector molecule to these sites can induce a conformational change in the active site, increasing or decreasing the enzyme's activity.
2) There are two main models that describe allosteric regulation - the concerted model where binding causes simultaneous changes in all subunits, and the sequential model where changes occur sequentially.
3) Allosteric enzymes exhibit sigmoidal kinetics curves rather than traditional hyperbolic curves due to their cooperative binding behavior. Positive allosteric effectors increase enzyme activity while negative effectors decrease it.
Glycolysis is a catabolic pathway that breaks down glucose to extract energy. It occurs in 10 steps and involves 2 phases. In the first phase, energy is invested to phosphorylate and cleave glucose. In the second phase, the products are further broken down with a net generation of ATP. Glycolysis converts one glucose into two pyruvate molecules, produces 2 NADH, uses 2 ATP and generates a net of 2 ATP per glucose. This pathway is regulated by controlling the activity of three key enzymes: hexokinase, phosphofructokinase, and pyruvate kinase.
Active sites of the enzyme is that point where substrate molecule bind for the chemical reaction. It is generally found on the surface of enzyme and in some enzyme it is a “Pit” like structure
The active site is a three-dimensional cleft formed by groups that come from different parts of the amino acid sequence
The active site takes up a relatively small part of the total volume of an enzyme
Active sites are clefts or crevices
Substrates are bound to enzymes by multiple weak attractions.
The specificity of binding depends on the precisely defined arrangement of atoms in an active site.
This file include these contents:
What is Triacylglycerol
Structure of triacylglycerol
Simple triacylglycerol
Mixed triacylglycerol
Biosynthesis of triacylglycerol
Utilization of triacylglycerol
Properties of triacylglycerol
Metabolic Fate of Pyruvate and Cori cycle and Alanine cycle Cori & Alanine cy...Amany Elsayed
Metabolic Fate of Pyruvate and Cori cycle and Alanine cycle Cori & Alanine cycle and Lactate Dehydrogenase Deficiency (LDHA) and Malate aspartate shuttle (cycle) and Glycerol phosphate shuttle and Mitochondrial shuttle
Carbohydrate metabolism denotes the various biochemical processes responsible for the formation, breakdown and interconversion of carbohydrates in living organisms. The most important carbohydrate is glucose, a simple sugar (monosaccharide) that is metabolized by nearly all known organisms.
This document discusses fatty acid biosynthesis. It begins with an introduction to fatty acids, their types and functions. It then describes the localization and key steps of biosynthesis, which involves condensation, reduction, dehydration and reduction reactions. The main enzymes involved are acetyl-CoA carboxylase and fatty acid synthase. Biosynthesis occurs via fatty acid synthase and produces palmitate in 7 cycles. It is regulated differently in plants versus animals. Fatty acids have important structural and biological functions in both plants and animals.
Glycoproteins are proteins that contain carbohydrate chains covalently attached. They can be O-linked, N-linked or GPI-anchored. Glycoproteins play important structural and functional roles like cell adhesion and acting as receptors. They are synthesized through a complex process in the endoplasmic reticulum and Golgi apparatus. Congenital disorders of glycosylation can occur from mutations affecting glycoprotein synthesis. Blood groups are also determined by glycoproteins on red blood cell surfaces.
Gluconeogenesis is the process by which glucose is synthesized from non-carbohydrate precursors in the liver and kidneys. It occurs mainly during periods of fasting and involves converting substrates like lactate, glycerol, and certain amino acids into glucose. The pathway overcomes three thermodynamic barriers of glycolysis through smaller successive steps. Regulation occurs through allosteric control of enzymes, hormonal control of fructose 2,6-bisphosphate levels, and transcriptional control of key genes like PEPCK and FOXO1. Together these mechanisms help direct carbon fluxes towards gluconeogenesis or glycolysis based on energy demands.
Glycogen is stored in the liver and muscles as a fuel reserve that can be easily mobilized to generate energy in the absence of oxygen. Glycogenesis is the process of glycogen synthesis from glucose, requiring enzymes like glycogen synthase. Glycogenolysis is the degradation of stored glycogen into glucose-6-phosphate and glucose by glycogen phosphorylase. The activities of glycogen synthase and phosphorylase are regulated by allosteric effectors and hormones like glucagon, epinephrine, and insulin to balance glycogen synthesis and breakdown. Genetic defects in glycogen metabolism can cause glycogen storage diseases.
Jayati Mishra presented on the de novo and salvage pathways of purines under the guidance of Pradip Hirapue. The presentation discussed:
1) The de novo pathway synthesizes purine nucleotides from simple precursors through a two-stage process forming IMP and then converting it to AMP or GMP.
2) The salvage pathway recycles purine bases and nucleosides obtained from the diet or cell turnover to form nucleotides.
3) Both pathways work together to synthesize the purine nucleotides needed for nucleic acid synthesis, with the salvage pathway playing a larger role in certain tissues.
1) Organisms require chemical energy stored in high-energy compounds for processes like muscle contraction and active transport.
2) High-energy compounds include ATP, phosphoenolpyruvate, and acetyl-CoA, which contain high-energy bonds like phosphoanhydride and thioester bonds.
3) ATP is the most common energy currency in cells. It stores and transports chemical energy through its high-energy phosphoanhydride bonds, which are hydrolyzed to fuel energetic reactions.
The document discusses different types of phospholipids and their structures. It describes glycerophospholipids, which contain glycerol, fatty acids, phosphoric acid, and a nitrogenous base. Major glycerophospholipids mentioned are phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, phosphatidylinositol, cardiolipin, and plasmalogen. Sphingophospholipids contain sphingosine instead of glycerol. Sphingomyelin is provided as an example. Key functions and tissue locations of different phospholipids are also summarized.
Sphingolipids are a class of lipids that include sphingomyelin, glycosphingolipids, and ceramide. They serve important structural and signaling functions in the cell membrane and participate in processes like cell growth, differentiation, and apoptosis. Sphingolipids are synthesized through de novo pathways or through the breakdown of sphingomyelin. Their metabolites, including ceramide, sphingosine-1-phosphate, and gangliosides act as second messengers and influence pathways such as PI3K/Akt and JNK. Sphingolipids are also components of lipid rafts and caveolae, which regulate protein activity and cellular signaling. Imbalances in sph
Inhibitors & uncouplers of oxidative phosphorylation & ETCDipesh Tamrakar
The document provides an overview of oxidative phosphorylation and electron transport chain inhibitors and uncouplers. It discusses key concepts like the Q-cycle, shuttle systems that transport cytosolic NADH into mitochondria, uncoupling proteins, and various inhibitors that target different parts of the electron transport chain and oxidative phosphorylation. Specific inhibitors and uncouplers mentioned include rotenone, antimycin, oligomycin, 2,4-dinitrophenol, and chloro carbonyl cyanide phenyl hydrazone. Thyroid hormones are also noted to play a role in regulating uncoupling proteins and thermogenesis.
Oligomeric enzymes consist of two or more polypeptide chains linked together by non-covalent interactions. Examples of oligomeric enzymes include lactate dehydrogenase, which is a tetramer, and tryptophan synthase, which contains two different subunits that each have distinct catalytic functions. Oligomeric enzyme organization allows for complex regulation through allostery and feedback inhibition not possible with monomeric enzymes.
The pentose phosphate pathway, also known as the hexose monophosphate shunt, is an alternative metabolic pathway to glycolysis that occurs in the cytosol. It is a complex pathway that helps generate NADPH for fatty acid synthesis and glutathione for antioxidant activity, as well as synthesizing ribose-5-phosphate for nucleotide and nucleic acid formation. Glucose-6-phosphate enters the pathway and is oxidized through a two-phase process involving dehydrogenation using NADP+ instead of NAD+. Several intermediates are formed and rearranged before regenerating glucose-6-phosphate and glyceraldehyde-3-phosphate to complete the nonoxidative phase. Genetic defects in glucose-6
This document summarizes the metabolic pathways of gluconeogenesis and glycogenolysis. It explains that gluconeogenesis synthesizes glucose from non-carbohydrate precursors through a series of steps that are largely the reverse of glycolysis, with three bypass reactions. Glycogenolysis breaks down glycogen stores in the liver and muscle into glucose through cleavage of glucose monomers by glycogen phosphorylase and subsequent conversion to glucose-6-phosphate. Both pathways are regulated by hormones like glucagon and epinephrine.
Triacylglycerols are the main form in which animals store fat and are composed of glycerol bonded to three fatty acid chains. They are insoluble in water and primarily function as energy reserves, being stored in adipose tissue. Triacylglycerols can be either simple, containing the same fatty acid at each position, or mixed, containing different fatty acids. They undergo hydrolysis for digestion and energy release and can become rancid if exposed to air, moisture, or bacteria through oxidative or hydrolytic processes. Antioxidants help prevent rancidity.
This document discusses allosteric enzymes, which have additional binding sites called allosteric sites that are distinct from the active site. Molecules that bind to these allosteric sites, called effectors, can cause a conformational change in the enzyme's structure that increases or decreases its catalytic activity. There are two main models that describe the mechanism of allostery: the concerted model proposed by Monod, Wyman, and Changeux and the sequential model proposed by Koshland, Nemethy, and Filmer. Allosteric effectors can be positive or negative, and allosteric regulation can be homotropic, involving the substrate, or heterotropic, involving a different molecule. Allo
This document discusses the biosynthesis of phospholipids. It begins by defining phospholipids as complex lipids containing phosphoric acid, fatty acids, nitrogenous bases, and alcohols. Phospholipids are synthesized primarily on the surfaces of the smooth endoplasmic reticulum and transported via vesicles to their destinations. There are two main types of phospholipids - glycerophospholipids and sphingolipids. Glycerophospholipids have asymmetrical fatty acid groups attached to carbon 1 and 2 of the glycerol backbone. They are synthesized by attaching two fatty acyl groups to glycerol-3-phosphate to form phosphatidic acid. The document then discusses the synthesis of specific phospholipids
1) Allosteric enzymes have additional sites called allosteric sites that are distinct from the active site. Binding of an effector molecule to these sites can induce a conformational change in the active site, increasing or decreasing the enzyme's activity.
2) There are two main models that describe allosteric regulation - the concerted model where binding causes simultaneous changes in all subunits, and the sequential model where changes occur sequentially.
3) Allosteric enzymes exhibit sigmoidal kinetics curves rather than traditional hyperbolic curves due to their cooperative binding behavior. Positive allosteric effectors increase enzyme activity while negative effectors decrease it.
Glycolysis is a catabolic pathway that breaks down glucose to extract energy. It occurs in 10 steps and involves 2 phases. In the first phase, energy is invested to phosphorylate and cleave glucose. In the second phase, the products are further broken down with a net generation of ATP. Glycolysis converts one glucose into two pyruvate molecules, produces 2 NADH, uses 2 ATP and generates a net of 2 ATP per glucose. This pathway is regulated by controlling the activity of three key enzymes: hexokinase, phosphofructokinase, and pyruvate kinase.
Active sites of the enzyme is that point where substrate molecule bind for the chemical reaction. It is generally found on the surface of enzyme and in some enzyme it is a “Pit” like structure
The active site is a three-dimensional cleft formed by groups that come from different parts of the amino acid sequence
The active site takes up a relatively small part of the total volume of an enzyme
Active sites are clefts or crevices
Substrates are bound to enzymes by multiple weak attractions.
The specificity of binding depends on the precisely defined arrangement of atoms in an active site.
This file include these contents:
What is Triacylglycerol
Structure of triacylglycerol
Simple triacylglycerol
Mixed triacylglycerol
Biosynthesis of triacylglycerol
Utilization of triacylglycerol
Properties of triacylglycerol
Metabolic Fate of Pyruvate and Cori cycle and Alanine cycle Cori & Alanine cy...Amany Elsayed
Metabolic Fate of Pyruvate and Cori cycle and Alanine cycle Cori & Alanine cycle and Lactate Dehydrogenase Deficiency (LDHA) and Malate aspartate shuttle (cycle) and Glycerol phosphate shuttle and Mitochondrial shuttle
Carbohydrate metabolism denotes the various biochemical processes responsible for the formation, breakdown and interconversion of carbohydrates in living organisms. The most important carbohydrate is glucose, a simple sugar (monosaccharide) that is metabolized by nearly all known organisms.
This document discusses carbohydrate metabolism. It begins by defining metabolism and describing the catabolic and anabolic reactions that break down and build up molecules. It then focuses on carbohydrate metabolism, noting that carbohydrates are the primary energy source and are broken down into monosaccharides like glucose before entering metabolic pathways. The document provides details on glycolysis and the multiple stages of glucose catabolism through both aerobic and anaerobic pathways to generate energy in the form of ATP.
1) The document discusses glucose metabolism and its importance as the preferred energy source for most tissues. It describes the normal ranges for fasting and post-meal blood glucose levels.
2) Glucose is used through several pathways - the major pathways of glycolysis and the citric acid cycle produce energy, while minor pathways produce other biologically active substances. Glucose can also be stored as glycogen or triglycerides, or excreted in urine when levels are too high.
3) Glycolysis and the citric acid cycle are described in detail, including their regulation and importance for energy production. Glycolysis occurs in the cytoplasm and is the first step for complete oxidation of glucose. The cit
1. The document discusses glucose metabolism and its importance as the preferred energy source for most tissues. It describes the major pathways of glucose oxidation including glycolysis and the citric acid cycle.
2. Glycolysis converts glucose to pyruvate, producing a small amount of energy. It is an important pathway that occurs in all cells. The citric acid cycle further oxidizes pyruvate and acetyl-CoA to carbon dioxide, producing more energy through ATP.
3. Hormones and enzymes regulate glycolysis, with insulin stimulating it and glucagon inhibiting it. Pyruvate occupies an important junction between metabolic pathways as it can enter the citric acid cycle or be used for other processes. Glucone
1) Glycolysis is the pathway that breaks down glucose to extract energy through the production of ATP and NADH. It occurs in the cytoplasm and does not require oxygen.
2) Under anaerobic conditions, fermentation pathways like lactic acid and alcohol fermentation allow glycolysis to continue by regenerating NAD+ from NADH.
3) The pentose phosphate pathway is an alternative glucose oxidation pathway that generates NADPH for biosynthesis rather than ATP. Gluconeogenesis synthesizes glucose from non-carbohydrate precursors like lactate, glycerol, and some amino acids, and occurs primarily in the liver.
This document discusses cellular respiration and the processes involved in breaking down glucose to generate energy in the form of ATP. It covers the key steps of glycolysis, which takes place in the cytoplasm, the Krebs cycle (also called the citric acid cycle), which occurs in the mitochondria, and the electron transport chain. The document outlines the learning objectives, provides an overview of cellular respiration, and describes in detail each step in breaking down glucose, including the generation of NADH and FADH2 to carry energy to the electron transport chain for oxidative phosphorylation to produce ATP.
Glycolysis is the pathway by which cells break down glucose to produce energy. It occurs in the cytoplasm and involves a 10 step process where glucose is oxidized to pyruvate, producing a small amount of ATP. In aerobic conditions, pyruvate further processes to produce more ATP through the Krebs cycle and electron transport chain. In anaerobic conditions, pyruvate is reduced to lactate, producing less ATP but allowing glycolysis to continue. Glycolysis is thus the first step of cellular respiration and an important source of energy production and precursor molecules in all cells.
Supplying a huge array of metabolic intermediates for biosynthetic reactions. Normally carbohydrate metabolism supplies more than half of the energy requirements of the body. In fact the brain largely depends upon carbohydrate
Carbohydrate metabolism comprises glycolysis, HMP shunt, Gluconeogenesis, Glycogenolysis, TCA cycle, with Glucose-6-phosphate dehydrogenase deficiency disorder.
Biochemistry lecture notes metabolism_glycolysis & pentose phosphate pathwayRengesh Balakrishnan
This document provides information on metabolic pathways and glycolysis. It discusses how metabolism involves enzyme-catalyzed reactions that make up metabolic pathways, converting precursors into products. Catabolic pathways break down molecules to release energy while anabolic pathways use this energy to build complex molecules. Glycolysis involves the breakdown of glucose into pyruvate, producing a small amount of ATP along with NADH. The fate of pyruvate depends on oxygen conditions, being oxidized to acetyl-CoA aerobically or reduced to lactate or ethanol anaerobically.
The document discusses carbohydrate metabolism, specifically glucose metabolism and the pathways involved in glucose oxidation and storage. It covers the following key points:
1) Glycolysis and the citric acid cycle are the two major pathways for glucose oxidation and energy production. Glycolysis occurs in the cytoplasm and citric acid cycle in the mitochondria.
2) Glycolysis converts glucose to pyruvate, producing a small amount of energy. Pyruvate can then enter the citric acid cycle or be converted to lactate.
3) The citric acid cycle further oxidizes acetyl groups from pyruvate, producing more energy through the electron transport chain.
The document provides information on metabolic pathways including glycolysis, the citric acid cycle, and the electron transport chain. It begins with an overview of glycolysis, including its two phases and location in the cytoplasm. Key details are provided on the regulation of three glycolytic enzymes: hexokinase, PFK-1, and pyruvate kinase. The document then discusses the fates of pyruvate, including its conversion to acetyl-CoA and entry into the citric acid cycle or fermentation pathways. An overview of the citric acid cycle follows, along with its regulation and role in ATP production. The electron transport chain is then introduced, along with the structures and functions of its four complexes. In summary
Metabolism is the network of chemical reactions that take place in living cells. It performs four main functions: obtaining energy, converting nutrients into macromolecules, assembling macromolecules, and degrading macromolecules. Metabolic pathways can be catabolic, anabolic, or amphibolic. Glycolysis converts glucose into pyruvate, generating a small amount of ATP. Pyruvate then undergoes oxidative decarboxylation to form acetyl-CoA, the entry point into the citric acid cycle. Diseases can impair glycolysis through deficiencies in enzymes like pyruvate kinase or disorders that cause lactic acidosis.
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Glycolysis and gluconeogenesis are two opposing metabolic pathways involved in glucose metabolism. Glycolysis breaks down glucose to pyruvate in the cytoplasm, generating a small amount of ATP. Gluconeogenesis requires energy in the form of ATP to synthesize glucose from non-carbohydrate precursors like lactate, glycerol, and some amino acids in the mitochondria and cytoplasm. Both pathways have important functions in energy production and maintaining intermediate metabolite levels, though they differ in their directionality, location in the cell, and energy requirements.
Glycolysis and the citric acid cycle are the main pathways for glucose metabolism and energy production in cells. Glycolysis breaks down glucose into pyruvate, generating a small amount of ATP. Pyruvate can then enter the citric acid cycle in mitochondria to be further oxidized, with electrons being transferred to oxygen through the electron transport chain. This generates a proton gradient that is used by ATP synthase to produce the majority of ATP through oxidative phosphorylation. Various pathways like gluconeogenesis, the pentose phosphate pathway, and glycogen metabolism also interact with glycolysis and the citric acid cycle to regulate glucose and energy homeostasis in the body.
Organisms use cellular respiration to harvest energy from organic molecules like glucose. There are two main types of cellular respiration - aerobic respiration which uses oxygen and occurs in mitochondria, and anaerobic respiration which does not use oxygen. Aerobic respiration breaks down glucose through glycolysis, the Krebs cycle, and the electron transport chain to produce much more ATP than anaerobic respiration. Anaerobic respiration involves fermentation and produces either ethanol or lactic acid instead of fully oxidizing glucose.
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Carbohydrates range widely in size and structure, and serve important functions in living organisms. They are produced from carbon dioxide and water through photosynthesis in plants. Monosaccharides are the simplest carbohydrates and include sugars like glucose, galactose, and fructose. In solution, monosaccharides typically form ring structures called pyranoses or furanoses with intramolecular bonds. The cyclic forms exist in equilibrium with linear forms, and reducing sugars can be detected through chemical tests involving oxidation-reduction reactions. Modern methods for quantifying sugars like glucose use enzyme-based colorimetric or electrochemical assays.
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This document provides an overview of proteins, amino acids, and peptides. It discusses how proteins perform important biological functions like catalysis, transport, and structure. It describes how amino acids are the building blocks of proteins and the different classifications of amino acids. It also summarizes how peptides are formed from amino acids and some of their functions. Finally, it covers common techniques used to separate, analyze, and study proteins like chromatography, electrophoresis, and spectroscopy.
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2. Central
Importance
of
Glucose
• Glucose
is
an
excellent
fuel
– Yields
good
amount
of
energy
upon
oxida8on
(ΔGcomplete
oxida8on
=
–2840
kJ/mol)
– Can
be
efficiently
stored
in
the
polymeric
form
– Many
organisms
and
8ssues
can
meet
their
energy
needs
on
glucose
only
• Glucose
is
a
versa8le
biochemical
precursor
– Bacteria
can
use
glucose
to
build
the
carbon
skeletons
of:
• All
the
amino
acids
• Membrane
lipids
• Nucleo8des
in
DNA
and
RNA
• Cofactors
needed
for
the
metabolism
3. Four
Major
Pathways
of
Glucose
U-liza-on
• Storage
– Can
be
stored
in
the
polymeric
form
(starch,
glycogen)
– When
there’s
excess
energy
• Glycolysis
– Generates
energy
via
oxida8on
of
glucose
– Short-‐term
energy
needs
• Pentose
Phosphate
Pathway
– Generates
NADPH
via
oxida8on
of
glucose
– For
detoxifica8on
and
the
biosynthesis
of
lipids
and
nucleo8des
• Synthesis
of
Structural
Polysaccharides
– For
example,
in
cell
walls
of
bacteria,
fungi,
and
plants
4. Glycolysis:
Importance
• Almost
universal
central
pathway
of
glucose
catabolism
• Sequence
of
enzyme-‐catalyzed
reac8ons
by
which
glucose
is
converted
into
pyruvate
• Pyruvate
can
be
further
aerobically
oxidized
• Pyruvate
can
be
used
as
a
precursor
in
biosynthesis
• Some
of
the
oxida8on-‐free
energy
is
captured
by
the
synthesis
of
ATP
and
NADH
• Research
of
glycolysis
played
a
large
role
in
the
development
of
modern
biochemistry
– Understanding
the
role
of
coenzymes
– Discovery
of
the
pivotal
role
of
ATP
– Development
of
methods
for
enzyme
purifica8on
– Inspira8on
for
the
next
genera8ons
of
biochemists
5. The
2
phases
of
glycolysis
• In
the
evolu8on
of
life,
glycolysis
probably
was
one
of
the
earliest
energy-‐yielding
pathways
• It
developed
before
photosynthesis,
when
the
atmosphere
was
s8ll
anaerobic
• Thus,
the
task
upon
early
organisms
was:
How
to
extract
free
energy
from
glucose
anaerobically?
• The
solu8on:
– First:
Ac8vate
it
by
phosphoryla8on
– Second:
Collect
energy
from
the
high-‐energy
metabolites
• Glycolysis
is
a
sequence
of
10
reac8ons,
5
are
preparatory
and
5
are
energy-‐yielding
6. Glycolysis:
The
Preparatory
Phase
For each
molecule of
glucose that
passes through
the preparatory
phase, two
molecules of
glyceraldehyde 3-
phosphate are
formed.
The
“lysis”
step of
glycolysis
2 ATP molecules are
used to raise the free
energy of the
intermediates
7. Glycolysis:
The
Payoff
Phase
4 ATP are produced per glucose
2 ATP/glucose is the net outcome
Energy is also conserved by the
formation of 2 NADH
molecules
3 types of chemical
transformations:
1. Breakage of glucose
backbone to yield pyruvate
(6C 2x 3C)
2. Formation of NADH by hydride
transfer to NAD+
3. Phosphorylation of ADP
by high phosphoryl group
potential compounds to make ATP
8. Chemical
Logic
of
Glycolysis
• How is the formation of NADH and ATP
coupled to glycolysis? (Free energy changes)
• Why are phosphorylated intermediates
important in glycolysis?
9. Glycolysis:
Fates
of
Pyruvate
• In most organisms pyruvate is metabolized via one of three catabolic
routes:
1. Citric acid cycle: pyruvate is oxidized and decarboxylated to release
CO2 (the electrons that are moving go through ETC in mito and are
used to make ATP; aerobic conditions)
2. Lactic acid fermentation: after vigorous exercise, [O2] in muscles is
low (hypoxia) NADH cannot be reoxidized to NAD+ for glycolysis
to continue pyruvate is reduced to lactate accepting electrons
from NADH (regenerating NAD+). Certain tissues (RBC and retina)
ferment pyruvate into lactate even under aerobic conditions
3. Alcohol fermentation: some yeasts and plants can ferment pyruvate
into ethanol and CO2 (important for beverage production and
baking)
• Pyruvate also some anabolic fates (can produce a.a. alanine or fatty
acids)
11. Anaerobic
Glycolysis:
Fermenta-on
• Genera&on
of
energy
(ATP)
without
consuming
oxygen
or
NAD+
• No
net
change
in
oxida8on
state
of
the
sugars
• Reduc8on
of
pyruvate
to
another
product
• Regenerates
NAD+
for
further
glycolysis
under
anaerobic
condi8ons
• The
process
is
used
in
the
produc8on
of
food
from
beer
to
yogurt
to
soy
sauce
12. Yeast
undergo
Ethanol
Fermenta-on
• Two-‐step
reduc8on
of
pyruvate
to
ethanol,
irreversible
• Humans
do
not
have
pyruvate
decarboxylase
• Humans
do
express
alcohol
dehydrogenase
for
ethanol
metabolism
• CO2
produced
in
the
first
step
is
responsible
for:
– carbona8on
in
beer
– dough
rising
when
baking
bread
• Both
steps
require
cofactors
– Pyruvate
decarboxylase:
Mg2+
and
thiamine
pyrophosphate
(TPP)
– Alcohol
dehydrogenase:
Zn2+
and
NADH
13. Animals
undergo
lac-c
acid
fermenta-on
• Reduc8on
of
pyruvate
to
lactate,
reversible
• Equilibrium
favors
lactate
forma8on
• During
strenuous
exercise,
lactate
builds
up
in
the
muscle
– Generally
less
than
1
minute
(even
most
toned
athletes
cannot
sprint
at
highest
speeds
for
more
than
a
minute!)
• The
acidifica8on
of
muscle
prevents
its
con8nuous
strenuous
work
14. Lac-c
Acid
Fermenta-on
The Cori Cycle
• No
net
change
in
NAD+
or
NADH
levels
• Lactate
can
be
transported
to
the
liver
to
be
converted
to
glucose
(the
Cori
cycle)
• Requires
a
recovery
8me
– High
amount
of
oxygen
consump8on
to
fuel
gluconeogenesis
– Restores
muscle
glycogen
stores
– Heavy
breathing
is
required
to
replenish
oxygen
to
repay
the
“oxygen
debt”
15. TPP
is
a
common
acetaldehyde
carrier
• Coenzyme
derived
from
vitamin
B1
(thiamine)
• Lack
of
B1
beriberi
(swelling,
pain,
paralysis
and
death)
• Cleavage
of
bonds
adjacent
to
carbonyl
groups
• Thiazolium
ring
of
TPP
stabilizes
carbanion
intermediates
by
providing
an
electrophilic
structure
into
which
the
carbanion
electrons
can
be
delocalized
by
resonance
“electron
sinks”
acidic proton
16.
17.
The
Preparatory
Phase
18. Step
1:
Phosphoryla-on
of
Glucose
• Ra8onale
– Traps
glucose
inside
the
cell
– Lowers
intracellular
glucose
concentra8on
to
allow
further
uptake
• This
process
uses
the
energy
of
ATP
• The
first
“priming”
reac8on
• Hexokinase
in
eukaryotes,
and
glucokinase
in
prokaryotes
and
liver
(isozymes:
2
or
more
enzymes
encoded
in
different
genes
but
catalyze
the
same
reac8on)
• Soluble
cytosolic
enzyme
(like
all
other
glycoly8c
enzymes)
• Nucleophilic
oxygen
at
C6
of
glucose
alacks
the
last
(γ)
phosphate
of
ATP
• ATP-‐bound
Mg2+
facilitates
this
process
by
shielding
the
nega8ve
charges
on
ATP
• Highly
thermodynamically
favorable/irreversible
– Regulated
mainly
by
substrate
inhibi8on
19. Step
2:
Phosphohexose
Isomeriza-on
• Ra8onale
– C1
of
fructose
is
easier
to
phospho-‐
rylate
by
PFK
– Allows
for
symmetrical
cleave
by
aldolase
• An
aldose
(glucose)
can
isomerize
into
a
ketose
(fructose)
via
an
enediol
intermediate
• The
isomeriza8on
is
catalyzed
by
the
ac8ve-‐site
glutamate,
via
general
acid/base
catalysis
• Slightly
thermodynamically
unfavorable/reversible
– Very
small
posi8ve
ΔG’o
indicates
that
the
reac8on
can
proceed
readily
in
either
direc8on
– Product
concentra8on
kept
low
to
drive
forward
21. Step
3:
2nd
Priming
Phosphoryla-on
• Ra8onale
– Further
ac8va8on
of
glc
– Allows
for
1
phosphate/
3-‐carbon
sugar
aper
step
4
• First
Commiled
Step
of
Glycolysis
– fructose
1,6-‐bisphosphate
is
commiled
to
become
pyruvate
and
yield
energy
whereas
g-‐6-‐p
and
f-‐6-‐p
have
other
possible
fates
• This
process
uses
the
energy
of
ATP
• Highly
thermodynamically
favorable/irreversible
• Phosphofructokinase-‐1
is
highly
regulated
– By
ATP,
ADP,
AMP,
fructose-‐2,6-‐bisphosphate,
and
other
metabolites
(detailed
next
chapter)
– Do
not
burn
glucose
if
there
is
plenty
of
ATP
22. Step
4:
Aldol
Cleavage
of
F-‐1,6-‐bP
• Ra8onale
– Cleavage
of
a
6-‐C
sugar
into
two
3-‐C
sugars
– High-‐energy
phosphate
sugars
are
3-‐C
sugars
• The
reverse
process
is
the
familiar
aldol
condensa8on
• Animal
and
plant
aldolases
employ
covalent
catalysis
• Fungal
and
bacterial
aldolases
employ
metal
ion
catalysis
• Thermodynamically
unfavorable/reversible
– The
actual
free
energy
change
is
small
and
therefore
the
reac8on
is
readily
reversible.
It
is
small
because
the
concentra8on
of
the
reactant
is
kept
low
– GAP
concentra8on
kept
low
to
pull
reac8on
forward
• What
is
the
mechanism
of
aldolase
(class
I)?
23. Step
5:
Triose
Phosphate
Interconversion
• Ra8onale:
– Allows
glycolysis
to
proceed
by
one
pathway
• Aldolase
creates
two
triose
phosphates:
– Dihydroxyacetone
Phosphate
(DHAP)
– Glyceraldehyde-‐3-‐Phosphate
(GAP)
• Only
GAP
is
the
substrate
for
the
next
enzyme
• DHAP
must
be
converted
to
GAP
• Similar
mechanism
as
phosphohexose
isomerase
• Completes
preparatory
phase
• Thermodynamically
unfavorable/reversible
– GAP
concentra8on
kept
low
to
pull
reac8on
forward
24.
The
Payoff
Phase
25. Step
6:
Oxida-on
of
GAP
• Ra8onale:
– Genera8on
of
a
high-‐
energy
phosphate
cpd
– Incorporates
inorganic
phosphate
– Which
allows
for
net
produc-on
of
ATP
via
glycolysis!
• First
energy-‐yielding
step
in
glycolysis
• Oxida8on
of
aldehyde
with
NAD+
gives
NADH
and
an
acyl
phosphate
(very
high
ΔG’o
=
–
49.3
kJ/mol)
• Ac8ve
site
cysteine
– Forms
high-‐energy
thioester
intermediate
– Subject
to
inac8va8on
by
oxida8ve
stress
• Thermodynamically
unfavorable/reversible
– Coupled
to
next
reac8on
to
pull
forward
• GAPDH
mechanism
(self
study)
26. Step
7:
1st
Produc-on
of
ATP
• Ra8onale:
– Substrate-‐level
phosphoryla8on
to
make
ATP
• 1,3-‐bisphosphoglycerate
is
a
high-‐energy
compound
–
can
donate
the
phosphate
group
to
ADP
to
make
ATP
• The
enzyme
is
named
aper
the
reverse
reac8on
• Substrate-‐level
phosphoryla-on:
the
fprma8on
of
ATP
by
group
transfer
from
a
substrate
• Highly
thermodynamically
favorable/reversible
– Is
reversible
because
of
coupling
to
GAPDH
reac8on
– Steps
6
and
7
are
strongly
coupled:
Glyceraldehyde
3-‐P
+
ADP
+
Pi
+
NAD+
3-‐phosphoglycerate
+
ATP
+
NADH
+
H+
ΔG’o
=
–12.2
kJ/mol
27. Step
8:
Migra-on
of
the
Phosphate
• Ra8onale:
– Be
able
to
form
high-‐energy
phosphate
compound
• Mutases
catalyze
the
(apparent)
migra8on
of
func8onal
groups
• One
of
the
ac8ve
site
his8dines
is
post-‐transla8onally
modified
to
phosphohis8dine
• Phosphohis8dine
donates
its
phosphate
to
O
at
C2
before
retrieving
another
phosphate
from
O
at
C3
• 2,3-‐bisphosphoglycerate
intermediate
• Note
that
the
phosphate
from
the
substrate
ends
up
bound
to
the
enzyme
at
the
end
of
the
reac8on
• Thermodynamically
unfavorable/reversible
• Reactant
concentra8on
kept
high
by
PGK
to
push
forward
28. Step
9:
Dehydra-on
of
2-‐PG
to
PEP
• Ra8onale
– Generate
a
high-‐energy
phosphate
compound
• 2-‐Phosphoglycerate
is
not
a
good
enough
phosphate
donor
(ΔG’o
=
–17.6
kJ/mol;
ΔG’o
PEP
=
–61.9
kJ/mol)
• Slightly
thermodynamically
unfavorable/reversible
• Product
concentra8on
kept
low
to
pull
forward
29. Step
10:
2nd
Produc-on
of
ATP
• Ra8onale
– Substrate-‐level
phosphoryla8on
to
make
ATP
– Net
produc8on
of
2
ATP/
glucose
• Loss
of
phosphate
from
PEP
yields
an
enol
that
tautomerizes
into
ketone
• Tautomeriza8on
• effec8vely
lowers
the
concentra8on
of
the
reac8on
product
• drives
the
reac8on
toward
ATP
forma8on
• Pyruvate
kinase
requires
divalent
metals
(Mg2+
or
Mn2+)
for
ac8vity
• Highly
thermodynamically
favorable/irreversible
• Regulated
by
ATP,
divalent
metals,
and
other
metabolites
31. Summary
of
Glycolysis
• Used:
– 1
glucose;
2
ATP;
2
NAD+
• Made:
– 2
pyruvate
• Various
different
fates
– 4
ATP
• Used
for
energy-‐requiring
processes
within
the
cell
– 2
NADH
• Must
be
reoxidized
to
NAD+
in
order
for
glycolysis
to
con8nue
• Glycolysis
is
heavily
regulated
– Ensure
proper
use
of
nutrients
– Ensure
produc8on
of
ATP
only
when
needed
– Under
anaerobic
condi8ons,
both
the
rate
and
the
total
amount
of
glucose
consump8on
are
many
8mes
greater
than
with
oxygen
present,
why???
Glucose + 2 NAD+ + 2 ADP + 2 Pi à 2 Pyruvate + 2 NADH + 2 H+ + 2 H2O+ 2 ATP
32.
33. Glycolysis
occurs
at
elevated
rates
in
tumor
cells
• Warburg
effect:
tumor
cells
carry
out
glycolysis
at
a
much
higher
rate
than
normal
cells
even
when
oxygen
is
available
(~10x)
• In
general,
the
more
aggressive
the
tumor,
the
greater
is
its
rate
of
glycolysis
• HIF-‐1
(hypoxia-‐inducible
transcrip8on
factor)
s8mulates
the
produc8on
of
at
least
8
glycoly8c
enzymes
and
glucose
transporters
when
the
oxygen
supply
is
limited
• HIF-‐1
also
s8mulates
the
produc8on
of
VEGF
(which
s8mulates
angiogenesis)
• Overreliance
of
tumors
on
glycolysis
suggests
a
possibility
for
an8cancer
therapy:
deplete
ATP
from
cancer
cells
by
blocking
glycolysis
• PET
scans
take
advantage
of
the
high
uptake
of
glucose
by
tumor
cells.
Used
to
pinpoint
cancers
34. Glucose
uptake
is
deficient
in
type
1
Diabetes
Mellitus
• Glucose
uptake
into
cells
is
mediated
by
GLUT
family
• GLUT1
&
GLUT2
(hepatocytes)
and
GLUT3
(brain
neurons)
are
always
present
in
the
plasma
membrane
of
these
cells
• GLUT4
(skeletal
and
cardiac
muscles
and
adipose)
only
move
to
the
plasma
membrane
in
response
to
an
insulin
signal
• Pa8ents
with
type
1
DM
have
too
few
β
cells
in
the
pancreas
(cannot
synthesize
enough
insulin)
heart,
muscles
and
fat
8ssues
cannot
uptake
glucose
hyperglycemia
(aper
carb-‐rich
meals)
• Fat
cells
turn
to
fat
metabolism
to
provide
alterna8ve
energy
forma8on
of
ketone
bodies
• In
untreated
type
1
DM
ketoacidosis
is
common
and
is
life-‐
threatening
• Reversed
by
insulin
injec8on
36. Feeder
Pathways
for
Glycolysis
• Glucose
molecules
are
cleaved
from
endogenous
glycogen
by
glycogen
phosphorylase
(phosphorolysis)
– Yielding
glucose-‐1-‐phosphate
• Dietary
starch
and
glycogen
are
cleaved
by
α-‐amylase
to
produce
oligosaccharides
and
subsequently
maltose
and
maltotriose
in
the
small
intes8ne,
by
pancrea8c
α-‐
amylase
(hydrolysis)
• Disaccharides
are
hydrolyzed
– Lactose:
glucose
and
galactose
(lactose
intolerance?)
– Sucrose:
glucose
and
fructose
– Fructose,
galactose,
and
mannose
enter
glycolysis
at
different
points
37. Gluconeogenesis:
Precursor
for
Carbohydrates
No&ce
that
mammals
cannot
convert
faIy
acids
to
sugars.
• Brain
and
nerve
cells,
RBC,
renal
medulla,
testes
an
embryonic
8ssue
use
only
glucose
as
the
energy
source
-‐
120
g
of
glucose
daily
(brain)
• Synthesizing
glucose
from
noncarbohydrate
precursors
–
gluconeogenesis
• In
mammals,
occurs
in
the
liver
(mainly)
and
in
renal
cortex
38. Glycolysis
vs.
Gluconeogenesis
Gluconeogenesis
occurs
mainly
in
the
liver.
Glycolysis
occurs
mainly
in
the
muscle
and
brain.
• Not
iden8cal
pathways
running
in
opposite
direc8ons
• 7
of
the
10
reac8ons
of
gluconeogenesis
are
the
reverse
of
glycolysis
• Both
are
irreversible
in
cells
• Both
occur
in
the
cytosol
(reciprocal
and
coordinated
regula8on)
• Opposing
pathways
that
are
both
thermodynamically
favorable
– Operate
in
opposite
direc8on
•
end
product
of
one
is
the
star8ng
cpd
of
the
other
• Reversible
reac8ons
are
used
by
both
pathways
• Irreversible
reac8on
of
glycolysis
must
be
bypassed
in
gluconeogenesis
– Highly
thermodynamically
favorable,
and
regulated
– Different
enzymes
in
the
different
pathways
– Differen8ally
regulated
to
prevent
a
fu8le
cycle
39. Pyruvate
to
Phosphoenolpyruvate
• Requires
two
energy-‐consuming
steps
• First
step,
pyruvate
carboxylase
converts
pyruvate
to
oxaloacetate
– Carboxyla8on
using
a
bio8n
cofactor
– Requires
transport
into
mitochondria
– First
regulatory
enzyme
in
gluconeogenesis
(acetyl
CoA
is
+ve
effector)
• Second
step,
phosphoenolpyruvate
carboxykinase
converts
oxaloacetate
to
PEP
– Phosphoryla8on
from
GTP
and
decarboxyla8on
– Occurs
in
mitochondria
or
cytosol
depending
on
the
organism
Carboxylation-decarboxylation
sequences activate pyruvate
40. Bio-n
is
a
CO2
Carrier
• Bio8n
is
covalently
alached
to
the
enzyme
through
an
amide
linkage
to
the
ε-‐amino
group
of
a
Lys
residue
• The
reac8on
occurs
in
two
phases
(at
two
different
sites):
• At
cataly8c
site
1,
bicarbonate
ion
is
converted
to
CO2
at
the
expense
of
ATP.
CO2
reacts
with
bio8n,
forming
carboxybio8nyl-‐enzyme
• The
long
arm
carries
the
CO2
of
carboxybio8nylenzyme
to
cataly8c
site
2
on
the
enzyme
surface,
where
CO2
is
released
and
reacts
with
the
pyruvate,
forming
oxaloacetate
• The
general
role
of
flexible
arms
in
carrying
reac8on
intermediates
between
enzyme
ac8ve
sites
41. Malate
dehydrogenase
• No
transporter
of
oxaloacetate
in
mitochondria
• OA
must
be
reduced
to
malate
by
mitochondrial
malate
dehydrogenase
using
NADH
OA
+
NADH
+
H+
L-‐malate
+
NAD+
• Very
low
[OA]
makes
the
ΔG
~
0
despite
the
high
ΔG’o
• In
cytosol,
L-‐malate
is
reoxidized
producing
NADH
L-‐malate
+
NAD+
OA
+
NADH
+
H+
• [NADH]/[NAD+]mito
>
[NADH]/[NAD+]cyto
105x
cytosolic
NADH
is
consumed
in
gluconeogenesis,
glucose
produc8on
cannot
con8nue
unless
NADH
is
available.
Moving
malate
from
mito
to
cytosol
moves
also
NADH
equivalents
to
allow
the
process
to
occur
42. Overall
bypass
reac-on
• OA
+
GTP
PEP
+
CO2
+
GDP
(PEP
carboxykinase)
• Reversible
under
cellular
condi8ons:
forma8on
of
one
high
energy
phosphate
is
balanced
by
the
hydrolysis
of
another
• Pyruvate
+
ATP
+
GTP
+
HCO3
-‐
PEP
+
CO2
+
ADP
+
GDP
+
Pi ΔG’o
=
0.9
kJ/mol
•
ΔG
for
the
reac8on
~
–25
kJ/mol
because
the
actual
cellular
[PEP]
is
very
low
the
reac8on
is
irreversible
in
vivo
43. Addi-onal
bypasses
• RBC
and
anaerobic
muscle
cells,
lactate
predominates
• Converted
to
pyruvate
by
LDH
• Produces
NADH
in
the
cytosol,
no
need
for
malate
conversion
• OA
is
decarboxylated
by
mito
PEP
carboxykinase
and
PEP
is
exported
from
mito
44. Addi-onal
Bypasses
• Catalyze
reverse
reac8on
of
opposing
step
in
glycolysis
• Are
irreversible
themselves
• Fructose
1,6-‐bisphosphate
Fructose
6-‐Phosphate
– By
fructose
bisphosphatase-‐1
(FBPase-‐1)
– Coordinately/oppositely
regulated
with
PFK
– A
hydrolysis
reac8on
with
ΔG’o
=
–
16.3
kJ/mol
• Glucose
6-‐phosphate
Glucose
– By
glucose
6-‐phosphatase
– A
hydrolysis
reac8on
with
ΔG’o
=
–
13.8
kJ/mol
– Enzyme
found
in
hepatocytes,
renal
medulla
and
intes8nal
epithelial
cells,
NOT
anywhere
else
(if
it
were
found
everywhere,
…
what
do
you
expect
would
happen?)
45. Gluconeogenesis
is
expensive
• Costs
4
ATP,
2
GTP,
and
2
NADH
• Not
the
reversal
of
the
conversion
of
pyr
to
glc
• But
physiologically
necessary
to
ensure
irreversibility
• Also,
there’s
a
need
to
keep
pyruvate
inside
the
cell
instead
of
secre8ng
it
outside.
Pyruvate
has
the
poten8al
to
make
more
than
10
ATP
per
full
oxida8on
of
pyruvate
• Brain,
nervous
system,
and
red
blood
cells
generate
ATP
ONLY
from
glucose
2
Pyruvate
+
4
ATP
+
2
GTP
+
2
NADH
+
2
H+
+
4
H2O
Glucose
+
4
ADP
+
2
GDP
+
6
Pi
+
2
NAD+
46. Precursors
for
Gluconeogenesis
• Glucose
can
be
produced
from
all
intermediates
of
the
CAC
(citrate,
isocitrate,
α-‐KG,
succinyl-‐CoA
,
succinate,
fumarate
and
malate)
since
all
of
them
can
undergo
oxida8on
to
OA
• Also,
most
a.a.
can
undergo
transforma8ons
to
pyruvate
or
CAC
intermediate,
and
therefore
has
the
poten8al
to
make
glucose:
i.e.
glucogenic
-‐
Only
Leu
and
Lys
are
non-‐glucogenic
-‐
Ala
and
Gln
are
par8cularly
important
glucogenic
a.a.
in
mammals
47. Precursors
for
Gluconeogenesis
• Animals
can
produce
glucose
from
sugars
or
proteins
and
parts
of
fat
(triacylglycerol)
– Sugars:
pyruvate,
lactate,
or
oxaloacetate
– Protein:
from
glucogenic
a.a.
– Glycerol:
the
breakdown
product
of
fats
can
be
used
aper
a
two
step
reac8on.
Glycerol
kinase
phosphorylates
it
and
the
oxida8on
of
the
central
C
yields
dihydroxyacetone
phosphate
(an
intermediate
in
gluconeogenesis)
• Animals
cannot
produce
glucose
from
faly
acids
– Product
of
faly
acid
degrada8on
is
acetyl-‐CoA
– Cannot
have
a
net
conversion
of
acetyl-‐CoA
to
oxaloacetate
(2
C
that
enter
the
CAC
are
removed
as
2CO2)
• Plants,
yeast,
and
many
bacteria
can
do
this
(the
glyoxylate
cycle),
thus
producing
glucose
from
faly
acids
48.
49. Pentose
Phosphate
Pathway
• Glc
6-‐P
has
another
catabolic
fate
which
leads
to
specialized
products
needed
by
cells
• The
main
products
are
NADPH
and
ribose
5-‐phosphate
• NADPH
is
an
electron
donor
– Reduc8ve
biosynthesis
of
faly
acids
and
steroids
(liver,
adipose,
gonads,
etc.)
– Repair
of
oxida8ve
damage
esp.
in
cells
directly
exposed
to
O2
(RBC,
cornea)
• Ribose-‐5-‐phosphate
is
a
biosynthe8c
precursor
of
nucleo8des
– Used
in
DNA
and
RNA
synthesis
esp.
in
rapidly
dividing
cells
(skin,
bone
marrow,
tumors,
etc.)
– Or
synthesis
of
some
coenzymes
(ATP,
NADH,
FADH )
51. Oxida-ve
phase
generates
NADPH
and
R-‐5-‐P
1. Oxida8on
of
G-‐6-‐P
to
δ-‐lactone
by
G6PD,
reduc8on
of
NADP+
2. Lactone
hydrolysis
by
lactonase
3. Oxida8on
and
decarboxyla8on
by
6-‐PG
dehydrogenase
to
produce
ribulose
5-‐P
4. Forma8on
of
ribose
5-‐P
by
phosphopentose
isomerase
• Pentose
pathway
ends
here
in
some
8ssues
Essentially
irreversible
52. Non-‐oxida-ve
phase
regenerates
G-‐6-‐P
from
R-‐5-‐P
• Used
in
8ssues
requiring
more
NADPH
than
R-‐5-‐P
(e.g.
liver
and
adipose)
• Six
5-‐C
sugar
phosphates
are
converted
into
five
6-‐C
ones,
allowing
con8nued
G6P
oxida8on
and
NADPH
produc8on
• Details
are
not
important,
but
remember
the
two
key
enzymes
unique
in
this
pathway:
transketolase
and
transaldolase
Wernicke-Korsakoff
syndrome: thiamine
deficiency exacerbated by
transketolase defect
53. Glycolysis,
gluconeogenesis
and
pentose
phosphate
pathway
• All
enzymes
of
PP
are
in
the
cytosol
• Glycolysis,
gluconeogenesis
and
PP
are
connected
through
several
shared
intermediates
and
enzymes:
glc
pyr
Depending on the
cell’s relative needs
for NADPH, ATP and
pentose phosphates
54. NADPH
regulates
par--oning
into
glycolysis
vs.
pentose
phosphate
pathway
G6P can enter glycolysis or PP depending
on the current needs to the cell and the
concentration of NADP+ and NADPH
55. Ques-on
3
(Take
home
exam)
Due:
NEXT
WEEK
(js-ban@birzeit.edu)
• Please
solve
ques-ons:
1. 14
(Arsenate
poisoning)
2. 16
(Niacin)
3. 18
(Clinical
symptoms
of
enzyme
deficiency)
4. 25
(Ethanol
affects
blood
glucose)
5. 28
(Phloridzin)
For
wriIen
answers,
I
prefer
to
have
them
typed
in
Word.
I
can
accept
the
assignment
in
one
file
sent
to
my
email.
For
answers
that
require
solving
mathema&cally,
you
can
either
type
them
or
write
them
down
and
scan
them.