Ch 13_lecture_presentation

PowerPoint® Lecture
Presentations prepared by
Bradley W. Christian,
McLennan Community
College
C H A P T E R
© 2016 Pearson Education, Inc.
Viruses,
Viroids, and
Prions
13

General Characteristics of Viruses
Learning Objective
13-1 Differentiate a virus from a bacterium.

General Characteristics of Viruses
• Obligatory intracellular parasites
• Require living host cells to multiply
• Contain DNA or RNA
• Contain a protein coat
• No ribosomes
• No ATP-generating mechanism

Table 13.1 Viruses and Bacteria Compared

Host Range
• The spectrum of host cells a virus can infect
• Most viruses infect only specific types of cells
in one host
• Determined by specific host attachment sites and
cellular factors
• Bacteriophages—viruses that infect bacteria
• Range from 20 nm to 1000 nm in length

Figure 13.1 Virus sizes.
Bacteriophages f2, MS2
Poliovirus
Rhinovirus
Adenovirus
Rabies virus
Prion
Bacteriophage T4
Tobacco mosaic virus
Viroid
Vaccinia virus
24 nm
30 nm
30 nm
90 nm
170 × 70 nm
200 × 20 nm
225 nm
250 × 18 nm
300 × 10 nm
300 × 200 × 100 nm
Bacteriophage M13
Ebola virus
300 nm
E. coli bacterium
3000 × 1000 nm
Plasma membrane
of red blood cell
10 nm thick
Human red blood cell
10,000 nm in diameter
Chlamydia bacterium
elementary body
800 × 10 nm
970 nm

Check Your Understanding
 How could the small size of viruses have helped
researchers detect viruses before the invention of
the electron microscope?
13-1

Viral Structure
Learning Objective
13-2 Describe the chemical and physical structure
of both an enveloped and a nonenveloped
virus.

Viral Structure
• Virion—complete, fully developed viral particle
• Nucleic acid—DNA or RNA can be single- or double-
stranded; linear or circular
• Capsid—protein coat made of capsomeres (subunits)
• Envelope—lipid, protein, and carbohydrate coating on
some viruses
• Spikes—projections from outer surface

Figure 13.2 Morphology of a nonenveloped polyhedral virus.
Nucleic acid
Capsomere Capsid

Figure 13.3 Morphology of an enveloped helical virus.
Nucleic acid
Capsomere
Spikes
Envelope

General Morphology
• Helical viruses—hollow, cylindrical capsid
• Polyhedral viruses—many-sided
• Enveloped viruses
• Complex viruses—complicated structures

Figure 13.4 Morphology of a helical virus.
Nucleic acid
Capsomere
Capsid

Figure 13.5 Morphology of complex viruses.
65 nm
Capsid (head)
DNA
Sheath
Tail fiber
Pin
Baseplate
A T-even bacteriophage
Orthopoxvirus

 Diagram a nonenveloped polyhedral virus that has
spikes.
13-2

Taxonomy of Viruses
Learning Objectives
13-3 Define viral species.
13-4 Give an example of a family, genus, and
common name for a virus.

Taxonomy of Viruses
• Genus names end in -virus
• Family names end in -viridae
• Order names end in -ales
• Viral species: a group of viruses sharing the
same genetic information and ecological niche
(host)
• Descriptive common names are used for species
• Subspecies are designated by a number

 How does a virus species differ from a bacterial
species?
13-3
 Attach the proper endings to Papilloma- to show
the family and genus that includes HPV, the
cause of cervical cancer.
13-4

Isolation, Cultivation, and Identification of
Viruses
Learning Objectives
13-5 Describe how bacteriophages are cultured.
13-6 Describe how animal viruses are cultured.
13-7 List three techniques used to identify viruses.

Growing Bacteriophages in the Laboratory
• Viruses must be grown in living cells
• Bacteriophages are grown in bacteria
• Bacteriophages form plaques, which are clearings on a
lawn of bacteria on the surface of agar
• Each plaque corresponds to a single virus; can be
expressed as plaque-forming units (PFU)

Figure 13.6 Viral plaques formed by bacteriophages.
Plaques

Growing Animal Viruses in the Laboratory
• In living animals
• In embryonated eggs
• Virus injected into the egg
• Viral growth is signaled by changes or death of the
embryo

Figure 13.7 Inoculation of an embryonated egg.
Air
sac
Shell
Amniotic
cavity
Chorioallantoic
membrane
Chorioallantoic
membrane
inoculation
Amniotic
inoculation
Allantoic
inoculation
Yolk sac
inoculationAllantoic
cavity
Albumin
Shell
membrane
Yolk
sac

Growing Animal Viruses in the Laboratory
• In cell cultures
• Tissues are treated with enzymes to separate cells
• Virally infected cells are detected via their deterioration,
known as the cytopathic effect (CPE)
• Continuous cell lines are used

Figure 13.8 Cell cultures.
A tissue is treated with enzymes
to separate the cells.
Cells are suspended
in culture medium.
Normal cells or primary cells grow in a monolayer across
the glass or plastic container. Transformed cells or
continuous cell cultures do not grow in a monolayer.
Normal
cells
Transformed
cells

Figure 13.9 The cytopathic effect of viruses.

Viral Identification
• Cytopathic effects
• Serological tests
• Western blotting—reaction of the virus with antibodies
• Nucleic acids
• RFLPs
• PCR

 What is the plaque method?
13-5
 Why are continuous cell lines of more practical
use than primary cell lines for culturing viruses?
13-6
 What tests could you use to identify influenza
virus in a patient?
13-7

Viral Multiplication
Learning Objectives
13-8 Describe the lytic cycle of T-even
bacteriophages.
13-9 Describe the lysogenic cycle of bacteriophage
lambda.
13-10 Compare and contrast the multiplication cycle
of DNA- and RNA-containing animal viruses.

Viral Multiplication
• For a virus to multiply:
• It must invade a host cell
• It must take over the host's metabolic machinery
• One-step growth curve

Figure 13.10 A viral one-step growth curve.

Multiplication of Bacteriophages
• Lytic cycle
• Phage causes lysis and death of the host cell
• Lysogenic cycle
• Phage DNA is incorporated in the host DNA
• Phage conversion
• Specialized transduction

PLAY Animation: Viral Replication: Virulent
Bacteriophages
Viral Replication: Virulent Bacteriophages

PLAY Animation: Viral Replication: Temperate
Bacteriophages
Viral Replication: Temperate Bacteriophages

T-Even Bacteriophages: The Lytic Cycle
• Attachment: phage attaches by the tail fibers to
the host cell
• Penetration: phage lysozyme opens the cell wall;
tail sheath contracts to force the tail core and DNA
into the cell
• Biosynthesis: production of phage DNA and
proteins
• Maturation: assembly of phage particles
• Release: phage lysozyme breaks the cell wall

Figure 13.11 The lytic cycle of a T-even bacteriophage.
Attachment: Phage attaches
to host cell.
Penetration: Phage penetrates
host cell and injects its DNA.
Biosynthesis: Phage DNA directs
synthesis of viral components by the
host cell.
Maturation: Viral components are
assembled into virions.
Release: Host cell lyses, and new
virions are released.
Bacterial
cell wall
Bacterial
chromosome
Capsid DNA
Capsid (head)
Sheath
Tail fiber
Baseplate
Pin
Cell wall
Plasma membrane
Tail
Sheath contracted
Tail core
Tail
DNA
Capsid
Tail fibers

Bacteriophage Lambda (λ): The Lysogenic
Cycle
• Lysogeny: phage remains latent
• Phage DNA incorporates into host cell DNA
• Inserted phage DNA is known as a prophage
• When the host cell replicates its chromosome, it also
replicates prophage DNA
• Results in phage conversion—the host cell exhibits
new properties

Figure 13.12 The lysogenic cycle of bacteriophage λ in E. coli.
Phage DNA
(double-stranded)
Phage attaches
to host cell and
injects DNA.
Bacterial
chromosome
Occasionally, the prophage may
excise from the bacterial chromosome
by another recombination event,
initiating a lytic cycle.
Many cell
divisions
Lysogenic bacterium
reproduces normally.
Phage DNA integrates within the
bacterial chromosome by recombination,
becoming a prophage.
Prophage
Phage DNA circularizes and enters
lytic cycle or lysogenic cycle.
New phage DNA and
proteins are synthesized
and assembled into virions.
Cell lyses, releasing
phage virions.
OR
Lysogenic
cycle
Lytic
cycle

Bacteriophage Lambda (λ): The Lysogenic
Cycle
• Specialized transduction
• Specific bacterial genes transferred to another
bacterium via a phage
• Changes genetic properties of the bacteria

Figure 13.13 Specialized transduction.
Prophage gal gene
Prophage exists in
galactose-using host
(containing the gal gene).
Phage genome excises,
carrying with it the adjacent gal
gene from the host.
Phage matures and cell lyses,
releasing phage carrying gal
gene.
gal gene
gal gene
Galactose-
positive
donor cell
Bacterial
DNA
Phage infects a cell that cannot
utilize galactose (lacking gal
gene).
Along with the prophage, the
bacterial gal gene becomes
integrated into the new host's
DNA.
Lysogenic cell can now
metabolize galactose.
Galactose-positive
recombinant cell
Galactose-
negative
recipient cell

PLAY Animation: Transduction: Generalized
Transduction
Transduction: Generalized Transduction

PLAY Animation: Transduction: Specialized
Transduction
Transduction: Specialized Transduction

 How do bacteriophages get nucleotides and
amino acids if they don't have any metabolic
enzymes?
13-8
 Vibrio cholerae produces toxin and is capable of
causing cholera only when it is lysogenic. What
does this mean?
13-9

Table 13.3 Bacteriophage and Animal Viral Multiplication Compared

Multiplication of Animal Viruses
• Attachment: viruses attach to the cell membrane
• Entry by receptor-mediated endocytosis or fusion
• Uncoating by viral or host enzymes
• Biosynthesis: production of nucleic acid and
proteins
• Maturation: nucleic acid and capsid proteins
assemble
• Release by budding (enveloped viruses) or
rupture

Figure 13.14 The entry of viruses into host cells.
Host plasma membrane
proteins at site of
receptor-mediated
endocytosis
Fusion of viral envelope
and plasma membrane
Entry of pig retrovirus by
receptor-mediated endocytosis.
Entry of herpesvirus by fusion.

Figure 13.20 Budding of an enveloped virus.
Viral capsid
Host cell plasma membrane
Viral protein
Bud
Bud
Envelope
Release by budding
Lentivirus

PLAY Animation: Viral Replication: Overview
Viral Replication: Overview

PLAY Animation: Viral Replication: Animal
Viruses
Viral Replication: Animal Viruses

The Biosynthesis of DNA Viruses
• DNA viruses replicate their DNA in the nucleus of
the host using viral enzymes
• Synthesize capsid in the cytoplasm using host cell
enzymes

Figure 13.15 Replication of a DNA-Containing Animal Virus.
Papovavirus
DNA
Capsid
Nucleus
Cytoplasm
Host cell
Capsid proteins
MATURATION
Virions
mature.
RELEASE
Virions are
released.
BIOSYNTHESIS
Viral DNA is replicated,
and some viral proteins
are made.
mRNA
Viral DNA
Capsid
proteins
ENTRY and
UNCOATING
Virion enters cell, and
its DNA is uncoated.
A papovavirus is a typical
DNA-containing virus that
attacks animal cells.
ATTACHMENT
Virion attaches
to host cell.
Late translation;
capsid proteins are
synthesized.
Viral replication in animals generally follows these steps: attachment, entry,
uncoating, biosynthesis of nucleic acids and proteins, maturation, and release.
Knowledge of viral replication phases is important for drug development strategies,
and for understanding disease pathology.
KEY CONCEPTS
A portion of viral DNA is
transcribed, producing
mRNA that encodes
"early" viral proteins.

• Adenoviridae
• Double-stranded DNA, nonenveloped
• Respiratory infections in humans
• Tumors in animals

Figure 13.16a DNA-containing animal viruses.
Capsomere
Mastadenovirus

• Poxviridae
• Double-stranded DNA, enveloped
• Cause skin lesions
• Vaccinia and smallpox viruses (Orthopoxvirus)

• Herpesviridae
• HHV-1 and HHV-2—Simplexvirus; cause cold sores
• HHV-3—Varicellovirus; causes chickenpox
• HHV-4—Lymphocryptovirus; causes mononucleosis
• HHV-5—Cytomegalovirus
• HHV-6 and HHV-7—Roseolovirus
• HHV-8—Rhadinovirus; causes Kaposi's sarcoma

Figure 13.16b DNA-containing animal viruses.
Capsomeres
Simplexvirus

• Papovaviridae
• Double-stranded DNA, nonenveloped
• Papillomavirus
• Causes warts
• Can transform cells and cause cancer

• Hepadnaviridae
• Hepatitis B virus
• Use reverse transcriptase to make DNA from RNA

The Biosynthesis of RNA Viruses
• Virus multiplies in the host cell's cytoplasm using
RNA-dependent RNA polymerase
• ssRNA; + (sense) strand
• Viral RNA serves as mRNA for protein synthesis
• ssRNA; – (antisense) strand
• Viral RNA is transcribed to a + strand to serve as mRNA
for protein synthesis
• dsRNA—double-stranded RNA

Figure 13.17a Pathways of multiplication used by various RNA-containing viruses.
Attachment
Capsid
RNA
Nucleus
CytoplasmHost cell
Entry
and uncoating
RNA replication by viral RNA–
dependent RNA polymerase
Translation and synthesis
of viral proteins
Maturation
and release
+ or sense
strand of
viral genome
– or antisense
strand of
viral genome
ss = single-stranded
ds = double-stranded
+ strand
Capsid
protein
mRNA is transcribed
from the – strand.
– strand is transcribed
from + viral genome.
Uncoating releases
viral RNA and proteins.
Viral
genome
(RNA)
Viral
protein
ssRNA;
+ or sense strand;
Picornaviridae
KEY

Figure 13.17b Pathways of multiplication used by various RNA-containing viruses.
of viral proteins
Maturation
and release
– strands are
incorporated
into capsid
Capsid
protein
Attachment
Capsid
RNA
Nucleus
Cytoplasm
Entry
and uncoating
Uncoating releases
Viral
genome
(RNA)
Viral
protein
Additional – strands are
transcribed from mRNA.
+ or sense
strand of
viral genome
– or antisense
strand of
viral genome
The + strand (mRNA) must first be
transcribed from the – viral genome
before proteins can be synthesized.
ssRNA; – or
antisense strand;
Rhabdoviridae
KEY
Host cell

Figure 13.17c Pathways of multiplication used by various RNA-containing viruses.
of viral proteins
Maturation
and release
Attachment
Capsid
RNA
Nucleus
Cytoplasm
Entry
and uncoating
+ or sense
strand of
viral genome
– or antisense
strand of
viral genome
KEY
Uncoating releases
Viral
genome
(RNA)
Viral
protein
RNA polymerase initiates production of
– strands. The mRNA and – strands form the
dsRNA that is incorporated as new viral genome.
Capsid proteins and RNA-
mRNA is produced inside the
capsid and released into the
cytoplasm of the host.
dsRNA; + or sense
strand with – or antisense
strand; Reoviridae
Host cell

• Picornaviridae
• Single-stranded RNA, + strand, nonenveloped
• Enterovirus
• Poliovirus and coxsackievirus
• Rhinovirus
• Common cold
• Hepatitis A virus

• Togaviridae
• Single-stranded RNA, + strand, enveloped
• Alphavirus
• Transmitted by arthropods; includes chikungunya
• Rubivirus
• Rubella

• Rhabdoviridae
• Single-stranded RNA, − strand, one RNA strand
• Lyssavirus
• Rabies
• Numerous animal diseases

• Reoviridae
• Double-stranded RNA, nonenveloped
• Reovirus (respiratory enteric orphan)
• Rotavirus (mild respiratory infections and gastroenteritis)

Biosynthesis of RNA Viruses That Use DNA
• Single-stranded RNA, produce DNA
• Use reverse transcriptase to produce DNA from the
viral genome
• Viral DNA integrates into the host chromosome as a
provirus
• Retroviridae
• Lentivirus (HIV)
• Oncoviruses

Figure 13.19 Multiplication and inheritance processes of the Retroviridae.
Reverse
transcriptase
Capsid Envelope
Virus
Host cell
Two identical +
strands of RNA
Retrovirus enters by fusion
between attachment spikes
and the host cell receptors.
Uncoating releases the two
viral RNA strands and the
viral enzymes reverse
transcriptase, integrase,
and protease.
DNA of one
of the host cell's
chromosomes
Mature retrovirus
leaves the host cell,
acquiring an
envelope and
attachment spikes
as it buds out. Viral
enzymes
Viral RNA
Viral DNA
Reverse transcriptase
copies viral RNA to
produce double-
stranded DNA.
Viral proteins are processed
by viral protease; some of the
viral proteins are moved
to the host plasma membrane.
Identical strands
of RNAViral
proteins
RNA
Provirus
Transcription of the provirus
may also occur, producing
RNA for new retrovirus
genomes and RNA that
encodes the retrovirus
capsid, enzymes, and
envelope proteins.
The new viral DNA
is transported into
the host cell's nucleus,
where it's integrated into
a host cell chromosome
as a provirus by viral
integrase. The provirus
may be replicated when
the host cell replicates.

Table 13.2 Families of Viruses That Affect Humans (1 of 4)

 Describe the principal events of attachment, entry,
uncoating, biosynthesis, maturation, and release
of an enveloped DNA-containing virus.
13-10

Viruses and Cancer
Learning Objectives
13-11 Define oncogene and transformed cell.
13-12 Discuss the relationship between DNA- and
RNA-containing viruses and cancer.

Viruses and Cancer
• Several types of cancer are caused by viruses
• May develop long after a viral infection
• Cancers caused by viruses are not contagious
• Sarcoma: cancer of connective tissue
• Adenocarcinomas: cancers of glandular
epithelial tissue

The Transformation of Normal Cells into Tumor
Cells
• Oncogenes transform normal cells into cancerous
cells
• Oncogenic viruses become integrated into the
host cell's DNA and induce tumors
• A transformed cell harbors a tumor-specific
transplant antigen (TSTA) on the surface and a
T antigen in the nucleus

DNA Oncogenic Viruses
• Adenoviridae
• Herpesviridae
• Epstein-Barr virus
• Poxviridae
• Papovaviridae
• Human papillomavirus
• Hepadnaviridae
• Hepatitis B virus

RNA Oncogenic Viruses
• Retroviridae
• Viral RNA is transcribed to DNA (using reverse
transcriptase), which can integrate into host DNA
• HTLV-1 and HTLV-2 cause adult T cell leukemia and
lymphoma

 What is a provirus?
13-11
 How can an RNA virus cause cancer if it doesn't
have DNA to insert into a cell's genome?
13-12

Latent Viral Infections and Persistent Viral
Infections
Learning Objectives
13-13 Provide an example of a latent viral infection.
13-14 Differentiate persistent viral infections from
latent viral infections.

Latent Viral Infections and Persistent Viral
Infections
• Latent virus remains in asymptomatic host cell for
long periods
• May reactivate due to changes in immunity
• Cold sores, shingles
• A persistent viral infection occurs gradually over
a long period; is generally fatal
• Subacute sclerosing panencephalitis (measles virus)

Figure 13.21 Latent and persistent viral infections.
Acute infection
Latent infection
Persistent infection

Table 13.5 Examples of Latent and Persistent Viral Infections in Humans

 Is shingles a persistent or latent infection?
13-13, 13-14

Prions
Learning Objective
13-15 Discuss how a protein can be infectious.

Prions
• Proteinaceous infectious particles
• Inherited and transmissible by ingestion,
transplant, and surgical instruments
• Spongiform encephalopathies
• "Mad cow disease"
• Creutzfeldt-Jakob disease (CJD)
• Gerstmann-Sträussler-Scheinker syndrome
• Fatal familial insomnia
• Sheep scrapie

Prions
• PrPC: normal cellular prion protein, on the cell
surface
• PrPSc: scrapie protein; accumulates in brain cells,
forming plaques

PLAY Animation: Prions: Overview
Prions: Overview

PLAY Animation: Prions: Characteristics
Prions: Characteristics

PLAY Animation: Prions: Diseases
Prions: Diseases

Figure 13.22 How a protein can be infectious.
PrPc produced by cells
is secreted to the cell
surface.
PrPSc may be acquired or
produced by an
altered PrPc gene.
PrPSc reacts with PrPc
on the cell surface.
PrPSc converts the PrPc
to PrPSc.
Lysosome
Endosome
PrPSc continues to accumulate
as the endosome contents are
transferred to lysosomes.
The result is cell death.
PrPSc accumulates in
endosomes.The new PrPSc is taken
in, possibly by receptor-
mediated endocytosis.
The new PrPSc converts
more PrPc.
PrPc
PrPSc

Plant Viruses and Viroids
Learning Objectives
13-16 Differentiate virus, viroid, and prion.
13-17 Describe the lytic cycle for a plant virus.

Plant Viruses and Viroids
• Plant viruses: enter through wounds or via
insects
• Plant cells are generally protected from disease by an
impermeable cell wall
• Viroids: short pieces of naked RNA
• Cause potato spindle tuber disease

Figure 13.23 Linear and circular potato spindle tuber viroid (PSTV).
PSTV

Table 13.6 Classification of Some Major Plant Viruses

 Contrast viroids and prions, and for each name a
disease it causes.
13-15, 13-16
 How do plant viruses enter host cells?
13-17

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