INTRODUCTION
HISTORY
NEED OF SYNCHRONIZATION
SYNCHRONOUS CULTURES CAN BE OBTAINED IN SEVERAL WAYS:
Physical fractionation .
Chemical appro ach
CENTRIFUGAL ELUTRIATION
Inhibition of DNA synthesis
Nutritional deprivation
SYNCHRONIZATION AT LOW TEMPERATURE
CELLULAR TOTIPOTENCY
SOME HIGHLIGHTS OF CELL SYNCHRONIZATION
REFERENCES
INTRODUCTION
HISTORY
NEED OF SYNCHRONIZATION
SYNCHRONOUS CULTURES CAN BE OBTAINED IN SEVERAL WAYS:
Physical fractionation .
Chemical appro ach
CENTRIFUGAL ELUTRIATION
Inhibition of DNA synthesis
Nutritional deprivation
SYNCHRONIZATION AT LOW TEMPERATURE
CELLULAR TOTIPOTENCY
SOME HIGHLIGHTS OF CELL SYNCHRONIZATION
REFERENCES
This presentation covers the introduction to Insect Cell Culture. Also covers its general information about cell culture practices followed in the lab. It covers culture media, the source of cells for culture and examples of the cell line with their culture conditions.
Introduction
Terminologies
Types of tissue culture
Applications
Culturing
Sub-culturing
Cryopreservation
Detection of contaminants
In vitro transformation of cells
Cell viability
Rules for working in the Lab
Advantages
Limitations
Primary and established cell line cultureKAUSHAL SAHU
Introduction
Primary Culture
Steps of Primary Culture
Isolation Of Tissue
Dissection And Disaggregation
Types Of Primary Culture
Primary Explants Culture
Enzymatic Disaggregation
Mechanical Disaggregation
Cell Line( Finite & Continuous)
Naming A Cell Line
Choosing A Cell Line
Maintenance Of Cell Line
Conclusion
Reference
This presentation is about the basics of cytotoxicity and the possible cellular fates a cell goes through and cellular and molecular level alterations. The nanoparticle related cytotoxicity and its emerging impacts in the environment and health and the disease caused by it is also discussed briefly.
INTRODUCTION
HISTORY
NEED OF SYNCHRONIZATION
TYPES OF SYNCHRONIZATION
(I)PHYSICAL CELL SEPARATION
(II)BLOCKADE
PHYSICAL Vs BLOCKADE SYNCHRONIZATION
CONCLUSION
REFFERENCE
It's about animal biotechnology Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines. ... When whole or partial tissues are isolated and maintained ex vivo, the procedure is termed primary tissue culture.
Biology and characterization of the cell cultureKAUSHAL SAHU
Introduction
History
Important terminology
Biology of culture cell
Characterization of culture cell
Application of animal culture
Conclusion
References
This presentation covers the introduction to Insect Cell Culture. Also covers its general information about cell culture practices followed in the lab. It covers culture media, the source of cells for culture and examples of the cell line with their culture conditions.
Introduction
Terminologies
Types of tissue culture
Applications
Culturing
Sub-culturing
Cryopreservation
Detection of contaminants
In vitro transformation of cells
Cell viability
Rules for working in the Lab
Advantages
Limitations
Primary and established cell line cultureKAUSHAL SAHU
Introduction
Primary Culture
Steps of Primary Culture
Isolation Of Tissue
Dissection And Disaggregation
Types Of Primary Culture
Primary Explants Culture
Enzymatic Disaggregation
Mechanical Disaggregation
Cell Line( Finite & Continuous)
Naming A Cell Line
Choosing A Cell Line
Maintenance Of Cell Line
Conclusion
Reference
This presentation is about the basics of cytotoxicity and the possible cellular fates a cell goes through and cellular and molecular level alterations. The nanoparticle related cytotoxicity and its emerging impacts in the environment and health and the disease caused by it is also discussed briefly.
INTRODUCTION
HISTORY
NEED OF SYNCHRONIZATION
TYPES OF SYNCHRONIZATION
(I)PHYSICAL CELL SEPARATION
(II)BLOCKADE
PHYSICAL Vs BLOCKADE SYNCHRONIZATION
CONCLUSION
REFFERENCE
It's about animal biotechnology Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines. ... When whole or partial tissues are isolated and maintained ex vivo, the procedure is termed primary tissue culture.
Biology and characterization of the cell cultureKAUSHAL SAHU
Introduction
History
Important terminology
Biology of culture cell
Characterization of culture cell
Application of animal culture
Conclusion
References
Flow cytometry (FCM) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument.
Fluorescence- Activated Cell Sorter is a powerful technique used in cell sorting, cell-cycle analysis etc.
The presentation gives a basic understanding of the principle of FACS, instrumentation, interpretation of results, applications, how to do cell-cycle analysis using FACS and various troubleshooting tips.
Radial immunodiffusion (RID) or Mancini method, Mancini immunodiffusion or single radial immunodiffusion (SRID) assay is an immunodiffusion technique used in immunology to determine the quantity or concentration of an antigen in a sample
Il 17 signaling pathway a new therapeutic targetsafoora pordel
Interleukin 17 (IL-17) and its closest relative, IL-17F, have recently drawn much attention in the field of immunology. IL-17 and IL-17F are expressed by a distinct type of T cells, T helper 17 cells and certain other lymphocytes. These cytokines play key regulatory roles in host defense and inflammatory diseases.
Clinical labs are important in diseases diagnosis, determination its severity and patient response to specific treatment. Diagnosis of any disease is first done by physical examination by physician and confirmed by lab diagnostic tests.
Immunofluorescence (IF) or cell imaging techniques rely on the use of antibodies to label a specific target antigen with a fluorescent dye (also called fluorophores or fluorochromes) such as fluorescein isothiocyanate (FITC).
CRP is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells.
Rheumatoid arthritis (RA) is an autoimmune disease that causes chronic inflammation of the joints.
Antibodies directed against the Fc fragment of immunoglobulin G (IgG) are called rheumatoid factors (RFs). They are heterogenous and usually composed of immunoglobulin M (IgM)
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
3. Cell separation:
is the process of removing one cell population from another within
a biological sample, such as blood or tissue.
Example uses of cell separation include:
•Capture of circulating tumor cells from blood
•Isolation of immune cells from peripheral blood
•…
4. Immune cell isolation refers to methods that enable the enrichment of immune cell
subsets by techniques such as:
erythrocyte lysis
magnetic isolation
density centrifugation
Sorting by flowcytometry
Cell Separation:
5. This MACS technology is based on MACS Microbeads (Antibodies coupled with Magnectic
Beads), MACS Separator and the MACS Columns.
The MACS separation process occurs within the MACS Columns. The MACS Separator acts as a
“magnetic bar” by inducing a strong permanent high-gradient magnetic field into the column
matrix. Target cells that are labelled with MACS Microbeads are attracted to the column walls by
the magnetic force (Positive Selection). Unlabelled cells (Negative Selection), are allowed to flow
and pass through to be collected at the other end via natural gravitational force. Hence, with
MACS technology, both Target and non-Target cell fractions can be collected and isolated with
high purity to be subjected to downstream experimental procedures immediately.
Magnetic-activated cell sorting (MACS)
6.
7. Target cells that are
labelled with MACS
Microbeads are attracted to
the column walls by the
magnetic force (Positive
Selection) and Unlabelled
cells (Negative Selection).
8. 1 2 3
Isolation of mononuclear cells from human peripheral blood by Ficoll-Paque density gradient.
density centrifugation
9.
10. F L O W C Y T O M E T R Y
L E C T U R E R : S . P O R D E L
M . S C . O F I M M U N O L O G Y
11. •Flow ~ cells in motion
•Cyto ~ cell
•Metry ~ measure
Measuring properties of cells while in
a fluid stream
WHAT IS FLOWCYTOMETRY?
13. How it works
1. Draw cells, with excess fluid, from test tube into machine.
2. Cells pass in single file past laser.
3. Laser hits cell and light is scattered.
4. Photomultiplier multiplies light intensity and a light sensor
measures the amount of light and scatter pattern.
5. Phenotyping, Size and granularity detection
14. Basics of Flowcytometry:
Fluidics: cells in suspension flow in single-file
through
Optics: an illuminated volume where cells scatter light and
emit fluorescence that is collected, filtered
and
Electronics: fluorescence converted to digital values that are
stored on a computer
17. WHY LOOK AT FSC V. SSC
• Since FSC ~ size and SSC ~ internal structure, a correlated
measurement between them can allow for differentiation of
cell types in a heterogenous cell population
FSC
SSC
Lymphocytes
Monocytes
Granulocytes
RBCs, Debris,
Dead Cells
23. Basics of Flowcytometry:
Fluidics: cells in suspension flow in single-file
through
Optics: an illuminated volume where cells scatter light and
emit fluorescence that is collected, filtered
and
Electronics: fluorescence converted to digital values that are
stored on a computer
24. Flow Cytometry Is A Powerful Technique For Characterizing Immune
Cells
• Allows For Detection Of Surface Markers Of Cells
• Allows For Detection Of Intracellular Factors
• Allows Detection Of Secreted Factors By Cells
• Allows For Detection Of DNA Content
25. Immune cell isolation refers to methods that enable the enrichment of immune cell
subsets by techniques such as:
erythrocyte lysis
magnetic isolation
density centrifugation
Sorting by flowcytometry
Cell Separation: