Dot blotting involves spotting small amounts of protein samples onto a nitrocellulose or PVDF membrane. The membrane is then blocked and incubated with primary and secondary antibodies. Finally, it is imaged using a chemiluminescent substrate. Dot blotting allows for a quick assay to test antibody binding and determine the appropriate primary antibody concentration for Western blot. It has advantages over Western blot in being faster and less expensive.