Cell proliferation is the process by which the number of cells increases through cell division and differentiation. It plays a key role in development and tissue homeostasis. There are several techniques to measure cell proliferation, including assays that detect proliferation markers, measure ATP or new RNA synthesis, track membrane integrity, or analyze DNA or metabolic activity. Creative Bioarray offers flexible and accurate cell proliferation assays using various techniques such as DNA synthesis assays with thymidine or BrdU labeling or metabolic assays measuring tetrazolium salts or Alamar Blue, which provide dose response curves and IC50 values with a fast turnaround time.

1. Cell Proliferation Assay Services

2. What is Cell Proliferation ? 01

3. Cell proliferation is the process that
results in an increase of the number of
cells, and is defined by the balance
between cell divisions and cell loss
through cell death or differentiation.

4. Cell proliferation is increased in tumours.

5. Cells are structural and functional unit of our body which
control and maintain the function of all unicellular and
multicellular organisms.
The process of cell proliferation and differentiation plays a key
role from the time of embryogenesis to development of whole
organism from single- or double- cell embryo and continues its
critical role in maintenance of adult tissue homoeostasis by
recycling the old cells with new cells.

6. Phases of the cell cycle
Mitosis is the main way for eukaryotes
to divide cells.
multicellular organisms in a mitotic
way to increase the number of
somatic cells.

7. Creative Bioarray cell
proliferation assay
advantages
02

8. Flexible
We chose suitable assay
method based on the
customer’s cell type and
requirements.
Accurate Sensitive Deliverables Fast
The data obtained
strongly correlates to
the cell number.
Capable of detecting
low cell numbers.
High-throughput, fast
turnaround time.
Dose response
curves and IC50
values.

9. Different cell
proliferation assay
technique available
03

10. Detecting proliferation markers
Measuring [ATP]
Tracking new RNA synthesis
Fluorescence-based cell
proliferation assays
Measures of membrane integrity
3D cell proliferation assays
We are capable of
performing different cell
proliferation assays based
on several concepts
DNA synthesis cell proliferation assays
Metabolic cell proliferation assays
Detecting proliferation markers

11. DNA synthesis cell proliferation assays
Principle: Proliferating cells incorporate 3H-
thymidine/BrdU labels into their nascent
DNA, which can be washed, adhered to
filters and then measured.
Dye Label: 3H-thymidine, BrdU
Feature: Traditional method, reliable
Application: This is suitable for
immunohistochemistry,
immunocytochemistry, in-cell ELISAs, flow
cytometry analysis and high-throughput
screening.

12. Metabolic cell proliferation assays
Principle: Measure of cell proliferation based on the metabolic
activity of a population of cells can be used. Tetrazolium salts or
Alamar Blue are compounds which become reduced in the
environment of metabolically active cells, forming a formazan dye
that subsequently changes the color of the media, which is caused
by increased activity of the enzyme lactate dehydrogenase during
proliferation. The absorption of the media-containing dye solution
can be read using a spectrophotometer or microplate reader in
low- or high-throughput configurations.
Dye Label: MTT, XTT, MTS, WST1, WST8
Feature: Mature method for cell proliferation assays, High-
throughput
Related Cell Proliferation Kits are available in Creative Bioarray.

13. 3D cell proliferation assays
Besides, Creative Bioarray also
provides 3D cell proliferation
assays by using Soft agar assay
with optimized growth and assay
conditions based on our
experiences with 3D cell culturing.
We can upgrade the standard
package to include 2D vs 3D
comparison.

14. Call us today at 631-626-9181, or email us at
info@creative-bioarray.com for a quotation or if any questions!
Our customer service representatives are available 24hr a day!
We thank you for choosing Creative Bioarray service.

15. Thank you！