Flow cytometry and fluorescence-activated cell sorting (FACS) are techniques that analyze and sort cells based on their optical and fluorescence characteristics. Flow cytometry works by passing cells in single file past a laser, detecting the light scattered and emitted. FACS allows cells to be sorted one by one into containers based on their light scattering and fluorescence properties measured using flow cytometry. The process involves hydrodynamically focusing cells into a stream, labeling with fluorescent markers, exciting with a laser, and using charged plates to deflect droplets containing sorted cells into collection tubes. These techniques are commonly used for cell analysis, sorting, and isolation in research and bioprocess applications.
Flow cytometry is a technique used in Cell Biology to analyze and measure the volume of cells suspended in a liquid with streamline flow, exposed to a laser beam.
Flow cytometry is a technique used in Cell Biology to analyze and measure the volume of cells suspended in a liquid with streamline flow, exposed to a laser beam.
Fluorescence- Activated Cell Sorter is a powerful technique used in cell sorting, cell-cycle analysis etc.
The presentation gives a basic understanding of the principle of FACS, instrumentation, interpretation of results, applications, how to do cell-cycle analysis using FACS and various troubleshooting tips.
An introduction to cell sorting by Flow Cytometry. Including a brief overview of the history of droplet based cell sorting as well as how the instruments work. Some information adressing the key criteria to achieving a good cell sort outcome is also included.
FLOW CYTOMETRY, PRINCIPLE, APPLICATION, USE IN HAEMATOLOGY, COMPONENT OF FLOW CYTOMETRY, DATA INTERPRETATION, DATA ANALYSIS, CELL SHORTING ADVANTAGES AND DISADVANTAGES, IMMUNOLOGICAL CLASSIFICATION OF ACUTE
LEUKEMIA
the slideshare is been made to get knowledge about flow cytometry it's introduction, working, construction mainly components used in the flow cytometry and its application to use
Fluorescence- Activated Cell Sorter is a powerful technique used in cell sorting, cell-cycle analysis etc.
The presentation gives a basic understanding of the principle of FACS, instrumentation, interpretation of results, applications, how to do cell-cycle analysis using FACS and various troubleshooting tips.
An introduction to cell sorting by Flow Cytometry. Including a brief overview of the history of droplet based cell sorting as well as how the instruments work. Some information adressing the key criteria to achieving a good cell sort outcome is also included.
FLOW CYTOMETRY, PRINCIPLE, APPLICATION, USE IN HAEMATOLOGY, COMPONENT OF FLOW CYTOMETRY, DATA INTERPRETATION, DATA ANALYSIS, CELL SHORTING ADVANTAGES AND DISADVANTAGES, IMMUNOLOGICAL CLASSIFICATION OF ACUTE
LEUKEMIA
the slideshare is been made to get knowledge about flow cytometry it's introduction, working, construction mainly components used in the flow cytometry and its application to use
The technique of flow cytometry is used to evaluate cells for a number of functions, such as cell counting, phenotyping, cell cycle analysis, and viability.
Flow cytometry (FCM) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument.
Introduction
Definition
Basic mechanism
Prerequisite of flow cytometer
Components of flow cytometry
Flow system
Optics system
Concept of scattering
Advantage
Limitation
Application
Conclusion
References
How to become a flow cytometry expert in 4 daysCJ Xia
In this how to become expert in application X series, Boster Bio presents the comprehensive information for you to get a head start on the subject. In this case, you will learn everything you need to know to get started on flow cytometry. You will learn how it works, how to choose the right antibody for FACS, how to gate, and many more.
Fluorescent activated cell sorting (FACS) is a specialized type of flow cytometry used for sorting and analyzing a heterogeneous mixture of cells into different sub- populations based on the specific light scattering and fluorescent characteristics (from the specific labels) of each cell. The number of measurable parameters that can be used by this technology to separate cell populations is immense – starting from simple surface immunophenotyping to metabolic functions, cell cycle status, redox state, and DNA content analysis to name a few.
Since its inception, FACS has been used extensively in biomedical research and clinical diagnostics and therapeutics. The most common usage of FACS is seen in:
Analysis of whole human blood for diagnosing diseases, immunophenotyping
Sorting different blood cell fractions for ex-vivo manipulations and/or transplantations
Immuno-phenotypic analysis of murine blood to identify transgenic/knockout animals
Sorting and analysis of a slew of cell lines for various biological assays
Characterization and isolation of rare cells types like adult stem cells and cancer initiating cells
In this presentation, you will learn everything you need to know to get started on flow cytometry. You will learn how it works, how to choose the right antibody for FACS, how to gate, and many more.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Introduction to AI for Nonprofits with Tapp NetworkTechSoup
Dive into the world of AI! Experts Jon Hill and Tareq Monaur will guide you through AI's role in enhancing nonprofit websites and basic marketing strategies, making it easy to understand and apply.
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
3. PRINCIPLE:
• The basic principles of flow cytometry is the
passage of cells in single file in front of a laser so
they can be
• detected
• counted
• sorted
• Cell components are fluorescently labelled and
then excited by a laser to emit light at varying
wavelengths. 3
5. MECHANISM:
1.Biological sample
2. label it with a fluorescent marker
3. cells move in a linear stream through a focused light source
(laser beam)
4. fluorescent molecule gets activated and emit light that is filtered
and detected by sensitive light detectors.
5. conversion of analog fluorescent signals to digital signals.
5
6. A SORTER CAN BE DIVIDED INTO 3
SUBSYSTEMS
• .
Fluidics
Optics
electronics
6
7. SUBUNITS
Fluidics:
to introduce and focus the cells for interrogation and
create a stable breakoff for sorting.
Optics:
to generate and collect the signal lights.
Electronics:
To convert the optical signals to proportional digital
signals process the signal and communicate with the
computer.
7
8. FLUIDICS:
Sample is
* introduced into running sheath.
* hydrodynamically focused into core
stream.
* sheath fluid and sample do not mix.
* reduction of cross section.
* sample flow rate adjustable.
8
9. TWO TYPES OF OPTICS:
Excitation optics:
it consists of * laser
* fibre optic cables
9
10. COLLECTION OPTICS:
• * fibre optic cables that direct the emitted light
to appropriate emission filter block.
• * filter that direct the signals in the emission
block to the appropriate photon multiplier tube
(PMT).
10
11. FSC
• Laser light is scattered in a
forward direction .
• the intensity of this signal
has been attributed to cell
size, refractive index
(membrane permeability).
• laser light is scattered at
900 to the axis of the laser
path.
• The intensity of this signal is
proportional to the amount
of cytosolic structure in the
cell (eg : granules ,cell
inclusions etc..,)
SSC
OPTICS:
11
13. FLOW CYTOMETRY IN
BIOPROCESS:
• It is used for cell analysis and quantification
especially both bacteria and yeast in food,
drink and pharmaceutical industries.
• This technique is used in microbial
fermentation monitoring and control as well as
in development of more accurate kinetic
models directed to bioprocesses optimisation.
13
14. APPLICATIONS OF FLOW
CYTOMETRY:
• Cell function
• DNA and RNA analysis
• Cell death
• Sorting and cell isolation
• Immunophenotyping
14
15. FLOW CYTOMETRY
• Commonly found in research labs ,
user friendly.
• Cannot sort cells.
• Intuitive software interfaces.
• More personalized ,designed for
researchers.
• Sort heterogeneous mixture of cells
into different population.
• Automated features.
FACS
15
COMPARISION:
16. FLUORESCENT ACTIVATED CELL
SORTING OF LIVE CELLS
• FACS is a particular form of cytometry that enables a
mixture of different cells to be sorted one by one by
one or more containers.
• cells are sorted according to their specific light
scattering and fluorescent characteristics.
16
18. PROCESS OF FACS:
• Individual cells are interrogated by the laser as
in a normal flow cytometer.
• The machine is setup so that each individual
cell that enters a single droplet as it leaves the
nozzle tip.
• This drop is given an electronic charge ,
depending on the fluorescence of the cell inside
the drop.
18
19. PROCESS OF FACS:
• Deflection plates attracts or repels the cells
accordingly into the collection tubes. For eg
• single FITC stained cell in a single droplet
would be given a positive charge and be
attracted to the right. Collection tube to the
right would collect all positively charged
FITC stained cell droplets.
19
20. PROCESS OF FACS:
• A Single PE stained cell in a single
droplet would be given a negative
charge and be attracted to the left.
Collection tubes to the left would collect
all negatively charged PE stained cell
droplets.
20