Biosafety level 2 provides guidelines for working safely with agents that pose moderate hazards. It involves standard practices like prohibiting eating or drinking in labs, as well as special practices like using biohazard signs and restricting access. Personal protective equipment is also required, such as lab coats and gloves. Laboratory facilities at BSL-2 require features like lockable doors, hand washing sinks, and controlled ventilation to contain hazards and protect researchers.

1. BIOSAFETY LEVEL 2

2. LIST OF CONTENTS
Introduction
Standard microbiological practices
Special practices
Safety equipments
Laboratory facilities (secondary barriers)

3. INTRODUCTION
What is biosafety?
It is a system for the safe handling of toxic and
dangerous biological and chemical substances.
In medicine
It refers to the levels of lab containment protocols
It measured as biosafety level(BSL) 1,2,3,4 in
rising order of danger.

4. BSL- 2
BSL-2 builds upon BSL-1
It includes microbes that are
associated with human disease
But rarely serious.
Example :salmonella, HIV Virus
Staphylococcus aureus

5. Routes of Infection
Through the mouth
 Eating, drinking and smoking in the laboratory
Mouth pipetting
Transfer of microorganisms to mouth by
contaminated fingers or articles

6. Continue…
Through the skin
Accidental inoculation with a hypodermic needle,
other sharp instrument or glass
Through the eye
 Transfer of microorganisms to eyes by
contaminated fingers
Through the lungs
Inhalation of airborne microorganisms

7. STANDARD MICROBIOLOGICAL
PRACTICES
•Common to all laboratories
•It will enhance:
1. Worker safety
2. Environmental protection

8. Continued…
Not permitted in laboratories:
 Eating
 Drinking
 Smoking
 Pipetting by mouth
 Handling contact lenses

9. Continued…
Never
 Bend or break the needles
 Discard needles or sharps into biological
waste bags
 Discard needles into regular trash

10. Continued…
Minimize the creation of splashes or aerosols
Decontamination of work surfaces
Proper decontamination methods used
Materials to be decontaminated outside of
laboratory:
in a durable, leak proof container
closed container, easy to transport

11. SPECIAL PRACTICES
“Restricted Access
Restricted Access at BSL2 means
Persons who are at increased risk of acquiring infection, or
for whom infection may have serious consequences, are
not allowed in the laboratory or animal rooms
There are entry requirements for
workers in the lab
Doors are kept closed during
experiments

12. Biohazard sign
A biohazard sign must be posted on the entrance to
the laboratory
Appropriate information to be posted includes
• the agent(s) in use,
•the biosafety level,
•the required immunizations,
•the investigator's name and telephone number,
•any personal protective equipment that
must be worn in the laboratory

13. Cont…
All equipment - hoods, freezers, incubators,
centrifuges, etc. that are used with biohazardous
materials in a BSL-2 lab must have a biohazard label.

14. SPECIAL PRACTICES
Use leak-proof transport containers

15. Cont …
 high degree of precaution with any contaminated sharp
items, including needles and syringes, slides, pipettes,
capillary tubes, and scalpels

16. Needles & Sharps Precautions
DON’T place needles or sharps in waste containers

17. Needles & Sharps Precautions
(Cont..)
DON’T touch broken glass with
hands
Use plasticware

18. SPECIAL PRACTICES (Cont ..)
Laboratory personnel receive appropriate
immunizations or tests for the agents handled
Training with annual updates
Biosafety manual specific to lab
Decontaminate work surfaces
Report spills and accidents
No animals in laboratories

19. Laboratory Facilities (Secondary
Barriers)
Requirements
• Laboratories have lockable doors
• Sink for hand washing
• Work surfaces easily cleaned
• Bench tops are impervious to water
• Sturdy furniture

20. Requirements

21. Continued…
Air flows into lab without re-circulation to non-
lab areas
• Windows fitted with fly screens
•Adequate illumination

22. Continued…
Signage on the door with
restricted access when work in
progress.

23. Continued…
Requirements
Location: separated from
public areas
Structure: normal construction
Ventilation: directional

24. THANK YOU 