This document discusses biosafety and biosafety levels. It defines biosafety as steps taken to protect humans, products, and the environment from biological hazards that may occur from research or commerce involving infectious organisms or genetically modified organisms. The document then describes the four biosafety levels established by the CDC - Biosafety Level 1 requires basic safety precautions; Biosafety Level 2 requires more extensive precautions for work with pathogens that pose moderate hazards; Biosafety Level 3 is for dangerous indigenous or exotic agents that may cause severe disease through inhalation; and Biosafety Level 4 contains the most hazardous pathogens and requires the highest level of containment.
A university researcher died from an infection caused by bacteria he was studying. The bacteria, Yersinia pestis, causes plague. An autopsy found the bacteria in his body but no obvious cause of death. More tests are planned as no other illnesses have been reported. Biosafety aims to reduce risks from exposure to infectious agents through standard practices, containment equipment, facility design and other principles outlined in publications like the Biosafety in Microbiological and Biomedical Laboratories manual. Risk assessments consider the organism, procedures, containment and other factors to determine appropriate biosafety levels and practices.
This document discusses biosafety principles for microbiology and biomedical laboratories. It defines biosafety and outlines key concepts like biohazards, biosafety levels, and the biohazard symbol. Biosafety aims to minimize health and environmental risks from hazardous biological materials through administrative controls, safety equipment, and facility design tailored to the risks involved. The document also notes emerging issues at the intersection of biosafety and biotechnology like genetically modified organisms, biosecurity, and bioethics.
The document provides an introduction to biosafety. It discusses biological hazards risk assessment and the factors considered in risk assessment such as agent hazards, laboratory procedure hazards, and the capability of laboratory staff. It also describes the classification of infectious microorganisms into four risk groups based on their hazardous characteristics and routes of transmission. Various biosafety levels from 1 to 4 are established depending on the risk factors involved. Primary and secondary containment barriers as well as safety equipment used at different biosafety levels are explained.
This document discusses biosafety levels and containment practices for handling biohazardous agents in laboratories. It describes three biosafety levels (BSL1-3) with increasing safety requirements according to the hazard level of the agents. BSL1 requires standard microbiological practices. BSL2 requires controlled access, biological safety cabinets for aerosol-generating procedures, personal protective equipment, and waste decontamination. BSL3 involves handling agents of high risk and requires additional containment such as physical separation of the laboratory, controlled access, required personal protective equipment, decontamination of all waste and vacuum lines, and use of biological safety cabinets for all procedures involving biohazardous materials.
The document discusses basic principles of biosafety. It begins by defining biosafety as safety from exposure to infectious agents. It then discusses biosafety levels 1-4 which provide increasing levels of containment for biological hazards. It also discusses important biosafety concepts like standard microbiological practices, safety equipment, facility design and construction that help prevent exposure to biological hazards in research and clinical settings.
This document discusses biosafety levels and guidelines for working with infectious agents in microbiology laboratories. It begins by explaining the importance of biosafety and outlining prohibited activities. It then describes the four biosafety levels from 1 to 4, with level 1 requiring the fewest precautions for non-dangerous agents, and level 4 requiring the strictest methods for dangerous agents. Each biosafety level is explained in terms of the types of infectious agents handled, standard practices, protective equipment and barriers required to handle those agents safely.
The document discusses biosafety principles and practices for protecting laboratory personnel and the environment from exposure to potentially infectious biological materials and agents, including determining the risks posed by different agents, assessing those risks, and establishing biosafety levels and containment procedures appropriate to the level of risk.
The document discusses biosafety levels (BSL) which are used to classify biological agents based on risk. There are four biosafety levels, with BSL-1 posing the lowest risk (ex. E. coli bacteria) and BSL-4 posing the highest risk (ex. Ebola virus). Each level has specific containment controls for laboratory practices, safety equipment, and facility construction required to safely work with the biological agents in that risk group. The summary outlines some of the key containment controls like personal protective equipment, biological safety cabinets, and facility access restrictions that distinguish the different biosafety levels.
A university researcher died from an infection caused by bacteria he was studying. The bacteria, Yersinia pestis, causes plague. An autopsy found the bacteria in his body but no obvious cause of death. More tests are planned as no other illnesses have been reported. Biosafety aims to reduce risks from exposure to infectious agents through standard practices, containment equipment, facility design and other principles outlined in publications like the Biosafety in Microbiological and Biomedical Laboratories manual. Risk assessments consider the organism, procedures, containment and other factors to determine appropriate biosafety levels and practices.
This document discusses biosafety principles for microbiology and biomedical laboratories. It defines biosafety and outlines key concepts like biohazards, biosafety levels, and the biohazard symbol. Biosafety aims to minimize health and environmental risks from hazardous biological materials through administrative controls, safety equipment, and facility design tailored to the risks involved. The document also notes emerging issues at the intersection of biosafety and biotechnology like genetically modified organisms, biosecurity, and bioethics.
The document provides an introduction to biosafety. It discusses biological hazards risk assessment and the factors considered in risk assessment such as agent hazards, laboratory procedure hazards, and the capability of laboratory staff. It also describes the classification of infectious microorganisms into four risk groups based on their hazardous characteristics and routes of transmission. Various biosafety levels from 1 to 4 are established depending on the risk factors involved. Primary and secondary containment barriers as well as safety equipment used at different biosafety levels are explained.
This document discusses biosafety levels and containment practices for handling biohazardous agents in laboratories. It describes three biosafety levels (BSL1-3) with increasing safety requirements according to the hazard level of the agents. BSL1 requires standard microbiological practices. BSL2 requires controlled access, biological safety cabinets for aerosol-generating procedures, personal protective equipment, and waste decontamination. BSL3 involves handling agents of high risk and requires additional containment such as physical separation of the laboratory, controlled access, required personal protective equipment, decontamination of all waste and vacuum lines, and use of biological safety cabinets for all procedures involving biohazardous materials.
The document discusses basic principles of biosafety. It begins by defining biosafety as safety from exposure to infectious agents. It then discusses biosafety levels 1-4 which provide increasing levels of containment for biological hazards. It also discusses important biosafety concepts like standard microbiological practices, safety equipment, facility design and construction that help prevent exposure to biological hazards in research and clinical settings.
This document discusses biosafety levels and guidelines for working with infectious agents in microbiology laboratories. It begins by explaining the importance of biosafety and outlining prohibited activities. It then describes the four biosafety levels from 1 to 4, with level 1 requiring the fewest precautions for non-dangerous agents, and level 4 requiring the strictest methods for dangerous agents. Each biosafety level is explained in terms of the types of infectious agents handled, standard practices, protective equipment and barriers required to handle those agents safely.
The document discusses biosafety principles and practices for protecting laboratory personnel and the environment from exposure to potentially infectious biological materials and agents, including determining the risks posed by different agents, assessing those risks, and establishing biosafety levels and containment procedures appropriate to the level of risk.
The document discusses biosafety levels (BSL) which are used to classify biological agents based on risk. There are four biosafety levels, with BSL-1 posing the lowest risk (ex. E. coli bacteria) and BSL-4 posing the highest risk (ex. Ebola virus). Each level has specific containment controls for laboratory practices, safety equipment, and facility construction required to safely work with the biological agents in that risk group. The summary outlines some of the key containment controls like personal protective equipment, biological safety cabinets, and facility access restrictions that distinguish the different biosafety levels.
This document discusses biosafety in microbiology laboratories. It describes the different biosafety levels from 1 to 4, with level 1 requiring the fewest precautions for agents that do not consistently cause disease, and level 4 requiring the strictest methods for dealing with highly dangerous agents. For each level, it outlines the standard practices, personal protective equipment, facility requirements and examples of agents worked with at that level. Biosafety levels help ensure the safe handling of pathogens to protect laboratory workers from acquiring infections.
The document provides an introduction to biosafety, explaining that it aims to reduce risk of exposure to infectious materials through proper safety precautions and procedures. It discusses the need for biosafety in laboratories processing infectious agents and around recombinant DNA to protect workers and the environment. The document also outlines different biosafety levels and associated practices, containment facilities, risk groups of pathogens, and considerations for risk assessments.
Module 3 biosafety principles & microbiologycal risk group 21 1-18EhealthMoHS
This document discusses biosafety principles and the classification of microorganisms by risk group. It describes the different biosafety levels from 1 to 4, which provide increasing levels of protection based on combinations of laboratory practices, safety equipment, and facility requirements. The biosafety levels are designed to safely work with biological agents according to their hazards. It also discusses the World Health Organization's classification of microorganisms into four risk groups based on their risk of disease and transmission. The appropriate biosafety level is selected by matching the containment measures to the risk group of the agent being handled.
This document discusses biosafety and biosecurity in laboratories. It defines biosafety as the combination of practices and procedures used to prevent exposure to pathogens and their accidental release. This includes containment principles and technologies. Biosecurity refers to security measures to prevent theft or misuse of pathogens. The document outlines the history of biosafety guidelines and regulations. It emphasizes the importance of training, awareness, and proper equipment and practices for managing biohazard risks to laboratory personnel, the public, and environment.
This document discusses biosafety and containment procedures in laboratories working with genetically engineered organisms (GEOs). It defines biosafety as precautions to reduce exposure to infectious materials and limit contamination. Containment includes both physical measures, like air filtration and waste disposal, and biological measures, like disabling organism survival outside the lab. Biosafety levels 1-4 are outlined based on pathogen risk, with higher levels requiring more stringent controls and facilities to isolate dangerous biological agents. Biosafety cabinets are also described as primary containment equipment.
The document provides a detailed overview on the basic principles of operating a biotech or micro laboratory along with basic techniques with which to handle organisms, chemicals &equipment and ensuring your own, your colleagues and your environment's safety.
The document discusses biosafety levels and biosafety cabinets. It defines the four biosafety levels from BSL-1 to BSL-4 based on the risk group of pathogens handled. It also explains the different types of biosafety cabinets (class I to III), how they provide varying levels of protection to the user, product and environment through HEPA filtration and pressure differentials, and standards for their design and testing.
This document provides an overview of biorisk management, which includes biosafety and biosecurity. It defines key terms and describes the three components of biorisk management: assessment, mitigation, and performance. Assessment involves identifying hazards and risks. Mitigation consists of engineering, administrative and personal protective controls. Performance ensures the system works through audits, inspections, and continuous improvement. The document also discusses the CWA 15793:2008 biorisk management system standard and its implementation.
Biosafety is the application of safety precautions that reduce a Laboratory based risk of exposure to a potentially infectious material and limit contamination of the working and surrounding environment.
The primary principle of biosafety is “Containment”.
Containment
The action of keeping harmful things under control and within limits
Or
A series of safe methods for managing infectious bacteria in the laboratory.
The document discusses biohazards and outlines procedures for ensuring environmental safety when working with biological materials. It defines biohazards as biological substances that threaten human health, such as viruses, bacteria, and toxins. Different levels of biocontainment are used depending on the risk level of the pathogens being handled, with Level 1 requiring minimal precautions and Level 4 the highest level of isolation for dangerous pathogens lacking vaccines or treatments. Proper use of warning signs, protective equipment, sterilization processes, and segregated work areas are emphasized for reducing risks of exposure or contamination.
1. The document discusses biosafety in biomedical research and the risks associated with pathogens escaping from laboratories. It notes several historical examples of laboratory acquired infections (LAIs) that have caused illness or death outside of labs.
2. Determining the appropriate biosafety level involves assessing the risk group of the pathogen, transmission routes, available treatments, and the types of procedures being conducted. Higher biosafety levels have more stringent laboratory practices, facilities, and personal protective equipment to prevent exposure and containment breaches.
3. Effective biosafety is founded on risk assessment and informed understanding of containment through practices like proper use of biosafety cabinets, personal protective equipment, and facility design. Compliance with biosaf
Occupational and bio safety in food industriesGeetika K. Gopi
This document discusses occupational health, safety, and bio safety in food industries. It covers the following key points:
Occupational health aims to control health hazards and prevent work-related diseases and accidents. Workers face a wide range of physical, chemical, and biological hazards. Safety measures include proper training, protective equipment, ventilation, sanitation, and medical services.
Bio safety has four containment levels to safely handle infectious agents, based on hazard assessment. Elements of containment protect workers through laboratory practices and safety equipment, as well as facility design and construction. Risk-based guidelines specify the appropriate containment level depending on the pathogen type and transmission risk. Trainings help ensure safe laboratory procedures.
The document discusses containment methods for safely handling biohazardous agents in laboratories. Primary containment refers to protecting laboratory personnel through good microbiological techniques and safety equipment like biological safety cabinets. Secondary containment protects the external environment through facility design and operational practices. The purpose of containment is to reduce exposure risks and prevent escape of potentially biohazardous agents.
The document discusses biosafety concepts and practices. It begins by defining biosafety as safety from exposure to infectious agents. It then discusses biosafety issues in various disciplines like agriculture, medicine, and chemistry. The rest of the document outlines biosafety concepts, levels, and practices based on guidance from the Biosafety in Microbiological and Biomedical Laboratories (BMBL) including standard microbiological practices, safety equipment, and facility design requirements for different biosafety levels from BSL-1 to BSL-4. It also discusses risk assessment and containment practices for working with various biological hazards.
Laboratory biosafety and biosecurity issues related to Brucella research and ...ILRI
Presented by Joseph P. Kozlovac at a workshop on an integrated approach to controlling brucellosis in Africa, Addis Ababa, Ethiopia, 29-31 January 2013.
Biosafety is the precautionary measure that reduce laboratory risk to exposure of microbe . This power point by Lamria Agnes Meilani base on WHO standard .
There are four biosafety levels that provide increasing levels of containment for biological agents. Biosafety level 1 is for work with well-characterized agents that do not cause disease in healthy adults. It requires standard microbiological practices and personal protective equipment like lab coats and gloves. Biosafety level 2 involves agents that pose moderate hazards and requires all BSL-1 practices plus additional containment like restricted access and use of biosafety cabinets. Biosafety level 3 is for serious and potentially lethal agents and requires BSL-2 practices plus additional containment like controlled access, required personal protective equipment, and specialized facility design with airflow controls and separate rooms. Biosafety level 4 is the highest level and involves dangerous and exotic agents
If you work in food growing, food processing, or food distribution, there are many concerns to consider about the presence of pathogens and contaminants in the same environment as your product. This presentation answers frequently asked questions about establishing a pathogen environmental monitoring program for your facility.
This document discusses laboratory-acquired infections (LAIs), which are defined as infections acquired through laboratory or laboratory-related activities. LAIs can occur through various routes of exposure like inhalation or contact with mucous membranes. Biological agents that cause LAIs are classified into four groups based on their risk, with Group 1 posing the lowest risk and Group 4 posing the highest. Groups 3 and 4 pathogens can cause severe disease and may spread to the community, although treatment is usually available for Group 3. A large 1976 survey found over 4,000 LAIs from 159 agents, with 10 agents causing over half of cases. Preventive measures like proper laboratory design, safe practices, and PPE can minimize LAI risks.
Upstream bioprocessing involves steps like isolation and selection of microorganisms, media preparation, inoculation and incubation. Downstream bioprocessing involves steps like product harvesting, extraction, purification, quality control and packaging. Major upstream steps are formulation of fermentation medium, sterilization, inoculum preparation and fermentation. Downstream steps include cell disruption, solid-liquid separation, concentration, purification, formulation and quality monitoring. The overall process aims to isolate the desired product from fermentation broth in pure form through various unit operations.
This presentation clearly explains you about the Biosafety Level 2.
Its includes the requirements, practices, organisms, equipment and uses of Biosafety level 2.
The document discusses biosafety levels (BSL) from 1-3. BSLs are determined by factors like risk of infection, nature of work, and agent characteristics. Each level has standardized practices and facilities appropriate for the associated risks. BSL1 applies to low-risk agents and requires basic precautions. BSL2 is for agents associated with human disease, requiring enhanced safety measures. BSL3 is for indigenous agents that can cause serious disease through inhalation and has strict controls, medical surveillance, and restricted access. Higher BSLs build upon practices of lower levels with increasing containment requirements to protect laboratory personnel and the public.
This document discusses biosafety in microbiology laboratories. It describes the different biosafety levels from 1 to 4, with level 1 requiring the fewest precautions for agents that do not consistently cause disease, and level 4 requiring the strictest methods for dealing with highly dangerous agents. For each level, it outlines the standard practices, personal protective equipment, facility requirements and examples of agents worked with at that level. Biosafety levels help ensure the safe handling of pathogens to protect laboratory workers from acquiring infections.
The document provides an introduction to biosafety, explaining that it aims to reduce risk of exposure to infectious materials through proper safety precautions and procedures. It discusses the need for biosafety in laboratories processing infectious agents and around recombinant DNA to protect workers and the environment. The document also outlines different biosafety levels and associated practices, containment facilities, risk groups of pathogens, and considerations for risk assessments.
Module 3 biosafety principles & microbiologycal risk group 21 1-18EhealthMoHS
This document discusses biosafety principles and the classification of microorganisms by risk group. It describes the different biosafety levels from 1 to 4, which provide increasing levels of protection based on combinations of laboratory practices, safety equipment, and facility requirements. The biosafety levels are designed to safely work with biological agents according to their hazards. It also discusses the World Health Organization's classification of microorganisms into four risk groups based on their risk of disease and transmission. The appropriate biosafety level is selected by matching the containment measures to the risk group of the agent being handled.
This document discusses biosafety and biosecurity in laboratories. It defines biosafety as the combination of practices and procedures used to prevent exposure to pathogens and their accidental release. This includes containment principles and technologies. Biosecurity refers to security measures to prevent theft or misuse of pathogens. The document outlines the history of biosafety guidelines and regulations. It emphasizes the importance of training, awareness, and proper equipment and practices for managing biohazard risks to laboratory personnel, the public, and environment.
This document discusses biosafety and containment procedures in laboratories working with genetically engineered organisms (GEOs). It defines biosafety as precautions to reduce exposure to infectious materials and limit contamination. Containment includes both physical measures, like air filtration and waste disposal, and biological measures, like disabling organism survival outside the lab. Biosafety levels 1-4 are outlined based on pathogen risk, with higher levels requiring more stringent controls and facilities to isolate dangerous biological agents. Biosafety cabinets are also described as primary containment equipment.
The document provides a detailed overview on the basic principles of operating a biotech or micro laboratory along with basic techniques with which to handle organisms, chemicals &equipment and ensuring your own, your colleagues and your environment's safety.
The document discusses biosafety levels and biosafety cabinets. It defines the four biosafety levels from BSL-1 to BSL-4 based on the risk group of pathogens handled. It also explains the different types of biosafety cabinets (class I to III), how they provide varying levels of protection to the user, product and environment through HEPA filtration and pressure differentials, and standards for their design and testing.
This document provides an overview of biorisk management, which includes biosafety and biosecurity. It defines key terms and describes the three components of biorisk management: assessment, mitigation, and performance. Assessment involves identifying hazards and risks. Mitigation consists of engineering, administrative and personal protective controls. Performance ensures the system works through audits, inspections, and continuous improvement. The document also discusses the CWA 15793:2008 biorisk management system standard and its implementation.
Biosafety is the application of safety precautions that reduce a Laboratory based risk of exposure to a potentially infectious material and limit contamination of the working and surrounding environment.
The primary principle of biosafety is “Containment”.
Containment
The action of keeping harmful things under control and within limits
Or
A series of safe methods for managing infectious bacteria in the laboratory.
The document discusses biohazards and outlines procedures for ensuring environmental safety when working with biological materials. It defines biohazards as biological substances that threaten human health, such as viruses, bacteria, and toxins. Different levels of biocontainment are used depending on the risk level of the pathogens being handled, with Level 1 requiring minimal precautions and Level 4 the highest level of isolation for dangerous pathogens lacking vaccines or treatments. Proper use of warning signs, protective equipment, sterilization processes, and segregated work areas are emphasized for reducing risks of exposure or contamination.
1. The document discusses biosafety in biomedical research and the risks associated with pathogens escaping from laboratories. It notes several historical examples of laboratory acquired infections (LAIs) that have caused illness or death outside of labs.
2. Determining the appropriate biosafety level involves assessing the risk group of the pathogen, transmission routes, available treatments, and the types of procedures being conducted. Higher biosafety levels have more stringent laboratory practices, facilities, and personal protective equipment to prevent exposure and containment breaches.
3. Effective biosafety is founded on risk assessment and informed understanding of containment through practices like proper use of biosafety cabinets, personal protective equipment, and facility design. Compliance with biosaf
Occupational and bio safety in food industriesGeetika K. Gopi
This document discusses occupational health, safety, and bio safety in food industries. It covers the following key points:
Occupational health aims to control health hazards and prevent work-related diseases and accidents. Workers face a wide range of physical, chemical, and biological hazards. Safety measures include proper training, protective equipment, ventilation, sanitation, and medical services.
Bio safety has four containment levels to safely handle infectious agents, based on hazard assessment. Elements of containment protect workers through laboratory practices and safety equipment, as well as facility design and construction. Risk-based guidelines specify the appropriate containment level depending on the pathogen type and transmission risk. Trainings help ensure safe laboratory procedures.
The document discusses containment methods for safely handling biohazardous agents in laboratories. Primary containment refers to protecting laboratory personnel through good microbiological techniques and safety equipment like biological safety cabinets. Secondary containment protects the external environment through facility design and operational practices. The purpose of containment is to reduce exposure risks and prevent escape of potentially biohazardous agents.
The document discusses biosafety concepts and practices. It begins by defining biosafety as safety from exposure to infectious agents. It then discusses biosafety issues in various disciplines like agriculture, medicine, and chemistry. The rest of the document outlines biosafety concepts, levels, and practices based on guidance from the Biosafety in Microbiological and Biomedical Laboratories (BMBL) including standard microbiological practices, safety equipment, and facility design requirements for different biosafety levels from BSL-1 to BSL-4. It also discusses risk assessment and containment practices for working with various biological hazards.
Laboratory biosafety and biosecurity issues related to Brucella research and ...ILRI
Presented by Joseph P. Kozlovac at a workshop on an integrated approach to controlling brucellosis in Africa, Addis Ababa, Ethiopia, 29-31 January 2013.
Biosafety is the precautionary measure that reduce laboratory risk to exposure of microbe . This power point by Lamria Agnes Meilani base on WHO standard .
There are four biosafety levels that provide increasing levels of containment for biological agents. Biosafety level 1 is for work with well-characterized agents that do not cause disease in healthy adults. It requires standard microbiological practices and personal protective equipment like lab coats and gloves. Biosafety level 2 involves agents that pose moderate hazards and requires all BSL-1 practices plus additional containment like restricted access and use of biosafety cabinets. Biosafety level 3 is for serious and potentially lethal agents and requires BSL-2 practices plus additional containment like controlled access, required personal protective equipment, and specialized facility design with airflow controls and separate rooms. Biosafety level 4 is the highest level and involves dangerous and exotic agents
If you work in food growing, food processing, or food distribution, there are many concerns to consider about the presence of pathogens and contaminants in the same environment as your product. This presentation answers frequently asked questions about establishing a pathogen environmental monitoring program for your facility.
This document discusses laboratory-acquired infections (LAIs), which are defined as infections acquired through laboratory or laboratory-related activities. LAIs can occur through various routes of exposure like inhalation or contact with mucous membranes. Biological agents that cause LAIs are classified into four groups based on their risk, with Group 1 posing the lowest risk and Group 4 posing the highest. Groups 3 and 4 pathogens can cause severe disease and may spread to the community, although treatment is usually available for Group 3. A large 1976 survey found over 4,000 LAIs from 159 agents, with 10 agents causing over half of cases. Preventive measures like proper laboratory design, safe practices, and PPE can minimize LAI risks.
Upstream bioprocessing involves steps like isolation and selection of microorganisms, media preparation, inoculation and incubation. Downstream bioprocessing involves steps like product harvesting, extraction, purification, quality control and packaging. Major upstream steps are formulation of fermentation medium, sterilization, inoculum preparation and fermentation. Downstream steps include cell disruption, solid-liquid separation, concentration, purification, formulation and quality monitoring. The overall process aims to isolate the desired product from fermentation broth in pure form through various unit operations.
This presentation clearly explains you about the Biosafety Level 2.
Its includes the requirements, practices, organisms, equipment and uses of Biosafety level 2.
The document discusses biosafety levels (BSL) from 1-3. BSLs are determined by factors like risk of infection, nature of work, and agent characteristics. Each level has standardized practices and facilities appropriate for the associated risks. BSL1 applies to low-risk agents and requires basic precautions. BSL2 is for agents associated with human disease, requiring enhanced safety measures. BSL3 is for indigenous agents that can cause serious disease through inhalation and has strict controls, medical surveillance, and restricted access. Higher BSLs build upon practices of lower levels with increasing containment requirements to protect laboratory personnel and the public.
This document discusses laboratory biosafety levels and the transfer of biological samples. It begins by outlining the objectives and presentation outline. It then introduces biosafety practices and discusses the four biosafety levels - BSL-1 to BSL-4 - in increasing order of risk. Each level is associated with different safety practices and containment facilities depending on the risk of pathogens handled. The document also discusses regulations for the safe transfer of biological samples between facilities, including packaging, labeling and documentation requirements. Material transfer agreements are identified as important for governing the transfer and use of biological materials.
Workplace safety is an important aspect to protect personnel against injury or serious accident.In case of animal cell culture safety takes a front seat due to nature of work i.e. handling of human cells and tissues, viruses with high potential to cause infections to humans and other adventitious micro organisms. This presentation presents various methods of safety to protect lab personnel from infectious biological agents.
deals with biosafety in medical labs. universal safety precautions included. Includes updated 8 categories and colour coding for BMW management. Being a budding microbiologist, kept it focused on microbiology lab
Biosafety refers to ensuring safety when working with biological organisms. This document discusses biosafety concepts and issues including containment levels, biosafety cabinets, and risk assessment. The four biosafety levels range from level 1 posing minimal risk to level 4 posing high individual risk without vaccines or treatments. Biosafety cabinets are used to protect workers and the environment, with class I protecting environment, class II protecting samples and environment, and class III providing maximum protection in BSL-4 labs. Risk assessment considers an organism's pathogenicity, virulence, proliferation ability, and transmission route. Guidelines for recombinant DNA research emphasize risk-based containment and avoiding unnecessary regulation.
The application of knowledge, techniques and equipment to prevent a personal laboratory and environmental exposure to potentially infectious agents or biohazard is known as biosafety.
Biosafety defines the containment conditions under which infectious agents can be safely manipulated.
The objective of containment is to confine biohazard and to reduce the potential exposure of the laboratory worker, persons outside of the laboratory, and the environment to potentially infectious agents.
The document discusses biosafety, which aims to reduce risks from infectious agents and genetically modified organisms. Biosafety involves containment conditions and preventing exposure of laboratory workers, the public, and environment. It defines four biosafety levels based on risk groups of biological agents, with higher levels requiring more stringent containment and personal protective equipment. Risk assessment involves identifying and analyzing hazards to determine potential adverse effects of biotechnology research.
This document summarizes biosafety guidelines for working with biological materials in a laboratory setting. It describes the various hazards associated with bio research, including hazards from pathogens and laboratory procedures. It provides classifications for pathogens based on their risk level. It also outlines containment procedures like good microbiological techniques, personal protective equipment, and different biosafety levels that should be followed to minimize risk of exposure, depending on the pathogen risk group. The goal is to protect researchers and prevent the spread of infections.
This document summarizes biosafety guidelines for working with biological materials in a laboratory setting. It describes the various hazards associated with bio research, including hazards from pathogens and laboratory procedures. It provides classifications for pathogens based on their risk level. It also outlines containment procedures like good microbiological techniques, personal protective equipment, and different biosafety levels that should be followed to minimize risk of exposure, depending on the pathogen risk group. The goal is to protect researchers and prevent the spread of infections.
This document provides information on laboratory safety practices. It discusses designating a safety officer, biosafety levels and practices, general safety rules and regulations, biosafety cabinets, and safe use of biosafety cabinets. Biosafety levels range from level 1 to level 4, with increasing containment practices required for higher levels due to more dangerous biological agents. Biosafety cabinets come in classes I-III and types A1, A2, B1, and B2, with each type providing different levels of protection for samples and/or personnel. Proper use of biosafety cabinets is important to maintain safe airflow.
The document discusses the history and development of biosafety levels for handling biological agents. It notes that the first biosafety cabinet was created in 1943 and that the CDC began specifying biosafety levels in 1964, establishing levels 1 through 4. It provides details on the standard practices, safety equipment, and facilities required for biosafety levels 1 through 4, with level 1 requiring the fewest containment procedures and level 4 the most stringent for dangerous infectious agents.
The document discusses containment methods for safely handling biohazardous agents in laboratories. Primary containment involves good microbiological techniques and safety equipment like biological safety cabinets to protect personnel and the immediate lab environment. Secondary containment uses facility design and practices to protect the external environment. Containment aims to reduce exposure risks for lab workers and prevent agents from escaping outside. The document then describes different biosafety levels based on the hazard level of the agents and appropriate safety measures for each level.
This document discusses biosafety and biocontainment levels. It describes the risks associated with biological research, including hazards from pathogens and laboratory procedures. Pathogens are classified into four risk groups based on infectivity and severity of disease. Containment levels including biological and physical containment help reduce exposure risks. Primary containment involves laboratory practices and equipment while secondary containment involves facility design. Facilities are designated as biosafety level I, II or III depending on the risk group of pathogens handled.
This document discusses biosafety guidelines for laboratories working with genetically modified organisms (GMOs). It outlines different levels of biosafety containment from levels 1 to 4, with higher levels required for more dangerous pathogens. Physical and biological containment methods are described, including air filtration, sterilization lights, waste disposal procedures, and making organisms unable to survive outside the lab. Guidelines for safe practices in biosafety level 1 and 2 labs are provided. Several databases for finding biosafety information are also mentioned.
This document provides an introduction to biosafety. It defines biosafety as safety from exposure to infectious agents according to the CDC. It then discusses the history of biosafety, including the first biosafety conference in 1955 and the establishment of biosafety levels 1 through 4 by the CDC. The document outlines the need for biosafety in laboratories working with infectious agents and describes the scope of biosafety across fields like medicine, agriculture and exobiology. It also covers biosafety issues in academic research, regulations, signage, hazardous materials, and provides details on the four biosafety levels based on pathogen risk.
The document discusses biological safety levels for working with biological agents. It begins with a brief history of biological safety conferences and standards being established in the United States in the 1950s and 1960s. It then provides details on the four biological safety levels (BSL), including standard practices, safety equipment and facility requirements for each level. BSL1 is for agents not known to cause disease, while each increasing level incorporates more containment practices for more dangerous agents, with BSL4 being for dangerous and exotic agents that pose a high risk of life-threatening disease.
The document defines biosafety levels and containment controls for working with biological agents. There are four biosafety levels that provide increasing containment based on the risk group of the agent. BSL-1 is for low risk agents and allows open bench work, while BSL-4 is for dangerous exotic agents and requires the highest level of containment like positive pressure suits. Each level has specific laboratory practices, safety equipment, and facility requirements to safely contain biological agents and protect laboratory workers from exposure.
safety data sheet, an introduction to cell culture, safety equipment, safe laboratory practices, ascetic techniques, sterile work area, good personal hygiene, sterile reagents and media, sterile handling, planning of cell culture labs.
Similar to Biosafety Levels by Francis and Prasoona .pptx (20)
Unlock the power of genomic exploration with our comprehensive presentation, "Genome Browsing in Bioinformatics." This engaging PowerPoint file provides an in-depth guide to navigating and analyzing genomes using bioinformatics tool like Biocyc.
This simple laboratory PPT was designed for UPES-SOHST students as a guide for illustrating the experiment mentioned above, kindly share to help someone learn
This simple laboratory PPT was designed for UPES-SOHST students as a guide for illustrating the experiment mentioned above, kindly share to help someone learn
This document provides an overview of isolating cellular proteins from bacterial broth. It begins by defining proteins and their important functions in cells. The objectives are to understand proteins, enzymes, and functional proteomics. The experimental work involves isolating total protein from bacterial broth by lysing cells, centrifuging to remove debris, and collecting the supernatant containing soluble proteins. The methodology describes growing and harvesting E. coli cells, washing and lysing them using a lysis buffer, centrifuging to remove debris, and collecting the protein-containing supernatant which can then be stored or used for further analysis. The overall aim is to isolate proteins from E. coli broth culture using cell lysis and centrifugation techniques.
As a microbiologist, these are 15 unique areas of Biotechnology you should be familiar with, especially at the master's level
Please Note: If you can't read any text, kindly download and view as ppt
This document discusses the prediction of different features of functional genes, including the promoter region, coding region, and regulatory elements. It outlines several computational methods and bioinformatic tools that can predict these features, such as GenScan, Augustus, and PROMO. Accurately predicting gene features has applications in gene therapy, drug development, and disease diagnosis by helping to identify new drug targets or diagnose genetic disorders, as seen with cystic fibrosis treatments targeting the CFTR gene.
Proteins are polymers of amino acids existing as a structural and functional unit. here we look at various bioinformatics tools that can be used to predict these protein structures from its known amino acid sequence.
This document discusses various bioinformatics tools and methods for identifying genes from genomic sequences. It begins by defining genes and genomes, then describes reference databases like RefSeq that are important for gene identification. It outlines the general workflow for gene identification, including obtaining sequences, preprocessing, annotation, prediction, and validation. Specific tools mentioned include GENSCAN, Glimmer, and Augustus for gene prediction, and BLAST for sequence alignment. The document also discusses identifying other genomic features like promoters, repeats, and open reading frames. It emphasizes that accurate gene identification requires both computational and experimental approaches.
Designing a nucleotide primer using bioinformatic tools on NCBI could be a good way to decide which primer to design for gene amplification. This presentation was designed for computational biology students at UPES to give a step by step method for designing a Primer on NCBI.
The purpose of this study is to understand better Acetogens, unique attributes, Mechanism of specific pathways and their roles in industrial application.
This document discusses the impact of the Human Genome Project on medical advancement in India. It provides background on the human genome and the goals and processes of the Human Genome Project. Completing the human genome sequence provides benefits like enabling the diagnosis and treatment of genetic diseases in India. However, challenges remain like determining gene functions and understanding complex genetic traits. Overall, the document argues that further utilizing genome sequencing can help India better understand and manage its burden of genetic diseases.
This document discusses biological nitrogen fixation, which is responsible for 65% of nitrogen used by humans through food. It occurs through nitrogen-fixing bacteria, which can be free-living like Azotobacter or symbiotic like Rhizobium that form nodules on legume roots. The bacteria contain the enzyme nitrogenase, which converts atmospheric nitrogen gas into ammonia in an oxygen-free environment within the nodules. The ammonia is then assimilated into amino acids and other biomolecules through a series of reactions.
More from University of Petroleum and Energy studies (16)
KEY Points of Leicester travel clinic In London doc.docxNX Healthcare
In order to protect visitors' safety and wellbeing, Travel Clinic Leicester offers a wide range of travel-related health treatments, including individualized counseling and vaccines. Our team of medical experts specializes in getting people ready for international travel, with a particular emphasis on vaccines and health consultations to prevent travel-related illnesses. We provide a range of travel-related services, such as health concerns unique to a trip, prevention of malaria, and travel-related medical supplies. Our clinic is dedicated to providing top-notch care, keeping abreast of the most recent recommendations for vaccinations and travel health precautions. The goal of Travel Clinic Leicester is to keep you safe and well-rested no matter what kind of travel you choose—business, pleasure, or adventure.
Unlocking the Secrets to Safe Patient Handling.pdfLift Ability
Furthermore, the time constraints and workload in healthcare settings can make it challenging for caregivers to prioritise safe patient handling Australia practices, leading to shortcuts and increased risks.
PET CT beginners Guide covers some of the underrepresented topics in PET CTMiadAlsulami
This lecture briefly covers some of the underrepresented topics in Molecular imaging with cases , such as:
- Primary pleural tumors and pleural metastases.
- Distinguishing between MPM and Talc Pleurodesis.
- Urological tumors.
- The role of FDG PET in NET.
Trauma Outpatient Center is a comprehensive facility dedicated to addressing mental health challenges and providing medication-assisted treatment. We offer a diverse range of services aimed at assisting individuals in overcoming addiction, mental health disorders, and related obstacles. Our team consists of seasoned professionals who are both experienced and compassionate, committed to delivering the highest standard of care to our clients. By utilizing evidence-based treatment methods, we strive to help our clients achieve their goals and lead healthier, more fulfilling lives.
Our mission is to provide a safe and supportive environment where our clients can receive the highest quality of care. We are dedicated to assisting our clients in reaching their objectives and improving their overall well-being. We prioritize our clients' needs and individualize treatment plans to ensure they receive tailored care. Our approach is rooted in evidence-based practices proven effective in treating addiction and mental health disorders.
TEST BANK FOR Health Assessment in Nursing 7th Edition by Weber Chapters 1 - ...rightmanforbloodline
TEST BANK FOR Health Assessment in Nursing 7th Edition by Weber Chapters 1 - 34.
TEST BANK FOR Health Assessment in Nursing 7th Edition by Weber Chapters 1 - 34.
TEST BANK FOR Health Assessment in Nursing 7th Edition by Weber Chapters 1 - 34.
Gemma Wean- Nutritional solution for Artemiasmuskaan0008
GEMMA Wean is a high end larval co-feeding and weaning diet aimed at Artemia optimisation and is fortified with a high level of proteins and phospholipids. GEMMA Wean provides the early weaned juveniles with dedicated fish nutrition and is an ideal follow on from GEMMA Micro or Artemia.
GEMMA Wean has an optimised nutritional balance and physical quality so that it flows more freely and spreads readily on the water surface. The balance of phospholipid classes to- gether with the production technology based on a low temperature extrusion process improve the physical aspect of the pellets while still retaining the high phospholipid content.
GEMMA Wean is available in 0.1mm, 0.2mm and 0.3mm. There is also a 0.5mm micro-pellet, GEMMA Wean Diamond, which covers the early nursery stage from post-weaning to pre-growing.
Chandrima Spa Ajman is one of the leading Massage Center in Ajman, which is open 24 hours exclusively for men. Being one of the most affordable Spa in Ajman, we offer Body to Body massage, Kerala Massage, Malayali Massage, Indian Massage, Pakistani Massage Russian massage, Thai massage, Swedish massage, Hot Stone Massage, Deep Tissue Massage, and many more. Indulge in the ultimate massage experience and book your appointment today. We are confident that you will leave our Massage spa feeling refreshed, rejuvenated, and ready to take on the world.
Visit : https://massagespaajman.com/
Call : 052 987 1315
Deep Leg Vein Thrombosis (DVT): Meaning, Causes, Symptoms, Treatment, and Mor...The Lifesciences Magazine
Deep Leg Vein Thrombosis occurs when a blood clot forms in one or more of the deep veins in the legs. These clots can impede blood flow, leading to severe complications.
Can Allopathy and Homeopathy Be Used Together in India.pdfDharma Homoeopathy
This article explores the potential for combining allopathy and homeopathy in India, examining the benefits, challenges, and the emerging field of integrative medicine.
MBC Support Group for Black Women – Insights in Genetic Testing.pdfbkling
Christina Spears, breast cancer genetic counselor at the Ohio State University Comprehensive Cancer Center, joined us for the MBC Support Group for Black Women to discuss the importance of genetic testing in communities of color and answer pressing questions.
LGBTQ+ Adults: Unique Opportunities and Inclusive Approaches to CareVITASAuthor
This webinar helps clinicians understand the unique healthcare needs of the LGBTQ+ community, primarily in relation to end-of-life care. Topics include social and cultural background and challenges, healthcare disparities, advanced care planning, and strategies for reaching the community and improving quality of care.
INFECTION OF THE BRAIN -ENCEPHALITIS ( PPT)blessyjannu21
Neurological system includes brain and spinal cord. It plays an important role in functioning of our body. Encephalitis is the inflammation of the brain. Causes include viral infections, infections from insect bites or an autoimmune reaction that affects the brain. It can be life-threatening or cause long-term complications. Treatment varies, but most people require hospitalization so they can receive intensive treatment, including life support.
TEST BANK For Accounting Information Systems, 3rd Edition by Vernon Richardso...rightmanforbloodline
TEST BANK For Accounting Information Systems, 3rd Edition by Vernon Richardson, Verified Chapters 1 - 18, Complete Newest Version
TEST BANK For Accounting Information Systems, 3rd Edition by Vernon Richardson, Verified Chapters 1 - 18, Complete Newest Version
TEST BANK For Accounting Information Systems, 3rd Edition by Vernon Richardson, Verified Chapters 1 - 18, Complete Newest Version
This particular slides consist of- what is Pneumothorax,what are it's causes and it's effect on body, risk factors, symptoms,complications, diagnosis and role of physiotherapy in it.
This slide is very helpful for physiotherapy students and also for other medical and healthcare students.
Here is a summary of Pneumothorax:
Pneumothorax, also known as a collapsed lung, is a condition that occurs when air leaks into the space between the lung and chest wall. This air buildup puts pressure on the lung, preventing it from expanding fully when you breathe. A pneumothorax can cause a complete or partial collapse of the lung.
2. Objectives of this Overview
Meaning of Biosafety
General safety practices
Laboratory Hazards
Biosafety Levels
• Requirements
• Standard microbiological practices
• Safety Equipment and waste disposal
• Uses
• Organisms
References
Thank you
3. What is Biosafety?
• Biosafety are all steps taken to protect human, products or
environment from biological hazard that might occur from
research and commerce of infectious or from genetically
modified organisms.
• The risks associated with the laboratory activities occur either
from the samples or the procedural requirements hence
application of the standard microbiological techniques and
employing facilities suitable for the risk level of the pathogen
helps to protect the researcher from laboratory-acquired
infections.
5. General safety practices.
Basic safety rules for laboratory conduct should be observed whenever
working in a laboratory. Many of the most common safety rules are
listed below;
• Know locations of laboratory safety showers, eyewash stations, and
fire extinguishers. The safety equipment may be located in the
hallway near the laboratory entrance.
• Know emergency exit routes.
• Minimize all chemical exposures.
• No horseplay will be tolerated.
• Assume that all chemicals of unknown toxicity are highly toxic.
• Post warning signs when unusual hazards, hazardous materials,
hazardous equipment, or other special conditions are present.
• Do not use mouth suction for pipetting or starting a siphon.
• Wash exposed areas of the skin prior to leaving the laboratory.
6. Biosafety Laboratories (BSL).
Biosafety laboratory levels are designed to identify various protective
measures that are to be taken in a laboratory setting to protect the
researchers, the environment, and the microorganisms.
• These levels are defined by the Central for Disease Control and
Prevention (CDC), where each of these levels is outlined with specific
practices and safety requirements.
• Biosafety level designations are based on the combination of the
design features, equipment, practices, and procedures required while
working with agents from the various risk groups.
• The allocation of a pathogenic agent to a biosafety level for laboratory
work must be based on the risk assessment.
• Such assessments take the risk group as well as other factors into
consideration while establishing the appropriate biosafety level. The
biosafety levels, thus, might differ from one region to another.
• As per the CDC, biosafety levels are of four types depending on the
risk associated with the microorganism and the facilities available. The
levels of containment range from the biosafety level 1 (BSL-1), which
is the lowest to the level 4 (BSL-4), which is the highest.
8. Biosafety Level 1 (BSL-1)
• Biosafety Level 1 is the level appropriate for work involving well-characterized
agents not known to consistently cause disease in immune-competent adult
humans and cause a minimal potential hazard to the laboratory personnel and the
environment.
• Biosafety level 1 is the lowest safety level, and the precautions required for the
level are thus limited and not as extensive.
• These laboratories provide general space in which work is done with viable agents
that are not associated with disease in healthy adults.
Requirements
• The BSL-1 laboratories are not necessarily separated from the general traffic in the
building.
• Most of the work is typically conducted on open bench tops using general
microbiological practices.
• Unique laboratory design or containment equipment are not required but may be
used depending on the risk assessment.
• Laboratory personnel must be provided with specific training in the procedures to
be conducted in the laboratory, which is then supervised by a scientist with
training in microbiology or related sciences.
9. • Standard microbiological practices
• The laboratory supervisor should implement the policies regarding the access
control to the laboratory.
• Laboratory personnel must wash their hands after working with potentially
hazardous materials and before leaving the laboratory.
• Activities like eating, drinking, smoking, handling contact lenses, applying
cosmetics, and storing food are not be permitted in laboratory areas.
• Mouth pipetting is prohibited; mechanical pipetting devices must be employed.
• All procedures to be conducted in the laboratory should be performed while
avoiding the creation of splashes and aerosols.
Safety practices
• There are no safety specific safety practices required for BSL-1.
10. Safety equipment and waste disposal for BSL-1
• Special containment devices like the Bio-safety Cabinets are not required for BSL-1.
• In order to prevent the contamination of personal clothing, protective laboratory coats,
gowns, or uniforms are recommended.
• While conducting tests with a high possibility of aerosol formation, protective eyewear
can be used.
Uses
• Biosafety Level-1 is commonly used while performing tests on microbial agents that are
not known to cause diseases in immune-compromised individuals.
• These laboratories include the laboratories used for teaching purposes in colleges and
training centers.
Organisms:
The common organisms that require Biosafety Level-1 containment include less
hazardous organisms like Agrobacterium radiobacter, Aspergillus niger, Bacillus
thuringiensis, Escherichia coli strain K12, Lactobacillus acidophilus, Micrococcus
leuteus, Neurospora crassa, Pseudomonas fluorescens, Serratia marcescens.
• However, the requirement of the biosafety level might differ depending on the risk
assessment of the pathogen.
11. Biosafety Level 2 (BSL-2)
• Biosafety level-2 laboratories are the laboratories that are used for the tasks involving
microbial agents of moderate potential hazards to the laboratory personnel, the
environment, and the agent.
• However, the infectious agents or the toxins might pose a moderate danger if
accidentally inhaled, swallowed, or exposed to the skin.
• The precautions associated with biosafety level-2 are comparatively more extensive than
BSL-1, but BSL-1 and BSL-2 laboratories are generally considered as basic laboratories.
Requirements
• BSL-2 laboratories like BSL-1 laboratories are not necessarily separated from the general
traffic patterns in the building.
• However, access into the laboratory is limited while BSL-2 experiments are in progress.
• The annual inspection of the laboratories is also an important part of the BSL-2
requirements. These might include changing the filters or replacement of some devices.
• The work is mostly conducted on sterilized bench tops except for some processes that
might form aerosols. The latter is conducted in safety cabinets.
• The precautions to be followed in BSL-2 include all the precautions of the BSL-1 and
some additional precautions.
12. • Standard microbiological practices
• All the laboratory personnel must wash their hands after using viable microorganisms and before leaving the
laboratory.
• Eating, drinking, smoking, and handling contact lenses in the laboratory are strictly prohibited.
• Mechanical pipetting should be done instead of mouth pipetting.
• All contaminated cultures, glassware, plastic ware, and biologically contaminated waste must be treated as bio-
hazards and thus, autoclaved.
• Work surfaces must be decontaminated with disinfectant at the end of the day or after any spills or splashes.
• Used hypodermic syringes and needles, Pasteur pipettes, razor blades, contaminated broken glass, and blood
vials are treated as medical waste and discarded in puncture-resistant sharps disposal containers.
Safety Practices
• People with increased risk of acquiring infections like the immune-compromised and pregnant individuals
should not be allowed to enter the BSL-2 laboratories while the laboratories are at work.
• An annual review of the BSL-2 manual should be done to update the guidelines.
• Documented policies and procedures should be established that limit the entrance to individuals who know of
the potential hazards and are appropriately trained.
• A biohazard symbol is placed on pieces of equipment where biohazardous materials are used or stored.
13. Safety Equipment and waste disposal for BSL-2
• Protective coats are to be worn while entering the laboratory and then removed and kept in the laboratory
post work.
• The laboratory design should be made such that it can be easily cleaned and decontaminated with minimum
nooks and corners.
• The laboratory doors should be closed whenever work with hazardous biomaterials is conducted.
• An autoclave must be available.
Uses
• Biosafety level-2 laboratories are mostly used for routine analysis and culture of moderately hazardous agents.
• Besides, some of the laboratories used for teaching and training purposes are also BSL-2 laboratories.
Organisms
• The organisms that require BSL-2 laboratories include the pathogenic strains of E.
coli, Staphylococcus, Salmonella, Plasmodium falciparum, Toxoplasma, and Herpes Simples Viruses.
• The allocation of organisms to the laboratories, however, might differ depending on the risk assessment.
14.
15. Biosafety Level 3 (BSL-3)
• Biosafety level 3 (BSL-3) is the level where work is performed with agents that may cause
severe or potentially lethal disease through inhalation or aerosol formation, to the
personnel, and may even contaminate the environment.
• The tasks performed in the BSL-3 laboratories involve indigenous or exotic agents where
the potential for infection by aerosols is high, and the disease may have lethal
consequences.
• Autoinoculation and ingestion present primary hazards to personnel working with these
agents at this level.
• Working in such laboratories require laboratory personnel with specific training in
handling pathogenic and potentially lethal agents, along with supervisors scientists
competent in handling infectious agents and associated procedures.
Requirements
• Biosafety Level 3 containment laboratories for animals and research are the most
challenging containment level facilities to design and operate.
• These laboratories should be certified for use before initial operation and subsequently
on an annual schedule or after a program change, renovation, or replacement of system
components that may affect the operating environment of the laboratory.
16. • BSL-3 laboratories are also called the containment laboratory as they require
containment equipment to protect the personnel, the microbial agent, and the
environment.
• The requirements for BSL-3 include all the requirements of the BSL-1 and BSL-2
laboratories, along with some additional design features and special equipment.
Standard Microbiological Practices
• The entry to the BSL-3 laboratories is limited to individuals with appropriate training in
handling BSL-3 organisms, all of whom are selected by the laboratory supervisor.
• Besides the general procedures and laboratory practices, the supervisor also formulates
additional policies to limit the entry to the laboratory.
• All the procedures to be conducted in the BSL-3 must be conducted within a biosafety
cabinet to prevent the exposure of the aerosols to the laboratory personnel.
• The work surfaces and sinks should be decontaminated once every work shift or after
any spills or splashes.
• The BSL-3 laboratories should be separated from the general traffic in a building to limit
entry into the laboratories at all times.
17. Safety Practices
• The doors of the BSL-3 laboratories are closed at all times with appropriate BSL-3 signs
outside the suite, along with a universal biohazard sign and emergency contact
information.
• Laboratory personnel must have medical surveillance and offered appropriate
immunizations for agents handled or potentially present in the laboratory.
• Each institution should consider the collection and storage of serum samples from at-risk
personnel.
• A laboratory-specific biosafety manual, which is available and accessible to all, must be
prepared and adopted as a policy.
• The laboratory supervisor must check for the demonstration of proficiency in standard
and special microbiological practices by all laboratory personnel before working with
BSL-3 agents.
• Potentially hazardous materials must be placed in a durable, leak-proof container or vial
during collection, processing, storage, or transport within a facility.
• All laboratory equipment should be routinely decontaminated after work or after any
spills or splashes.
• The laboratory biosafety manual must define procedures t be adopted in the case of
exposure to infectious materials, and these should be treated accordingly.
• No work in the BSL-3 laboratories should be conducted on an open bench or an open
vessel. All the activities involving the infectious agents must be conducted within
Biosafety cabinets or other physical containment devices.
18. Safety Equipment and waste disposal for BSL-3
• Biosafety cabinets are to be used for the manipulation of all infectious agents.
• Individual protection gears like personal protective equipment, coats, gloves, and respiratory
protection should be worn while entering the laboratories and then removed before leaving.
• The air flowing in the laboratory shouldn’t be recirculated to any area of the laboratory and
should be HEPA-filtered prior to being discharged to the outside.
• The filters, manuals, equipment, vacuum pipes, autoclaves, etc. should be revised and
reviewed annually.
Uses
• BSL-3 laboratories are used for clinical, diagnostic, teaching, research, or production facilities.
• These laboratories are used for the handling and manipulation of highly infectious agents that
prose direct severe effects on the health of the personnel.
• These are used for the studies regarding the effects of infectious agents and various toxins
and their effects.
Organisms
• The pathogens that require BSL-3 laboratories include HIV, H1N1 flu, Yersinia
pestis, Mycobacterium tuberculosis, SARS, Rabies Virus, West Nile Virus, Ricketts, etc.
• The placement of the organisms in different Biosafety levels, however, might defer and should
also be determined after risk assessment.vg
19.
20. Biosafety Level 4 (BSL-4)
• Biosafety level 4 is the highest level that is employed while
working with dangerous infectious agents that present a high
individual as well as environmental risk in the form of life-
threatening disease, aerosol transmission, or unknown risk of
transmission.
• The BSL-4 laboratories are often used while handling and
manipulating Risk Group 4 pathogens that are extremely
dangerous, with no known vaccines or therapies, and require
extreme precautions during work.
• The BSL-4 laboratories are of two types; cabinet laboratory
where all the work is performed in a Class III biosafety cabinet
or similar physical containment with very carefully formulated
precautions and suit laboratory where all the laboratory
personnel are required to wear full-body, air-supplied suits
protective gears in the form of PPEs.
21. Requirements
• The requirements of BSL-4 laboratories are extensive with specific
laboratory design, training procedures, and highly protective
equipment and personal gears.
• These laboratories should be certified for use before initial operation
and subsequently on an annual schedule or after a program change,
renovation, or replacement of system components that may affect the
operating environment of the laboratory.
• BSL-4 laboratories are also termed the maximum containment
laboratories as they have secondary barriers to prevent hazardous
materials from escaping into the environment.
• The BSL-4 laboratories should follow the requirements of all BSL-1,
BSL-2, and BSL-3, along with additional specific precautions.
22. Standard microbiological practices
• No work conducted within the BSL-4 should be done on an open
bench or an open vessel.
• The work stations, equipment, and sinks should be sterilized post
work.
• The laboratory personnel should be in protective gear that might
include full-body PPEs, gloves, masks, and coats.
• The doors of the laboratories should be closed at all times with the
laboratory placed away from the general traffic in the building.
• Activities like drinking, eating, mouth pipetting should be avoided at
all costs.
• Only people that are trained in handling the BSL-4 organisms and the
equipment in the laboratory should be allowed into the laboratory.
23. Safety Practices
• Viable or intact biological materials to be removed from the Class III cabinet
in a BSL-4 are transferred in a nonbreakable, sealed primary container with a
nonbreakable, sealed secondary container.
• No materials, except the biological materials that are to remain in a viable or
intact state, are removed from the BSL-4 laboratory unless they have been
autoclaved or decontaminated before they leave the facility.
• Only individuals whose presence in the facility is required for microbiological
processes or support purposes are authorized to enter. Individuals that are at
increased risk of acquiring an infection or for whom infection may be
unusually hazardous are not allowed in the laboratory.
• Personnel can enter and leave the facility only after the clothing change and
through the shower rooms.
• When the BSL-4 laboratory is at work or when infectious materials or
infected animals are present in the laboratory, a hazard warning sign, along
with the universal biohazard symbol, is placed on all access doors.
• A system is set up for reporting laboratory accidents, exposures, and the
medical surveillance of potential laboratory-associated illnesses.
24. Safety equipment and waste disposal
for BSL-4
• A Class III biological safety cabinet or Class I or II biological safety
cabinets used in conjunction with one-piece personnel suits
ventilated by a life support system are to be present in a BSL-4
while conducting all procedures within the facility.
• Walls, floors, and ceilings of the laboratories must form a sealed
internal shell which facilitates fumigation and is animal and
insect-proof.
• A double-doored autoclave is placed for decontaminating
materials passing out of the facility.
• The exhaust air from the facility is filtered through HEPA filters
before being discharged to the outside so as to prevent its entry
into occupied buildings and air intakes.
25. Uses
• BSL-4 laboratories are used for diagnostic and research work on
easily transmitted pathogens, causing fatal diseases.
• These laboratories are used for new and unknown pathogenic
microbes, for which no vaccines or therapies are available.
• They are also used for clinical and production facilities that
require highly sophisticated techniques and advanced
processes.
• Organisms
• The BSL-4 level pathogens include the risk group IV organisms
like Ebola virus, SARS-CoV-2, Central European Encephalitis
virus, Hemorrhagic viruses, etc.
26. References
• National Research Council (US) Committee on Hazardous Biological Substances in the
Laboratory. Biosafety In The Laboratory: Prudent Practices for the Handling and Disposal of
Infectious Materials. Washington (DC): National Academies Press (US); 1989. Appendix A,
Biosafety in Microbiological and Biomedical Laboratories. Available
from: https://www.ncbi.nlm.nih.gov/books/NBK218631/
• Mourya, D. T., Yadav, P. D., Majumdar, T. D., Chauhan, D. S., & Katoch, V. M. (2014). Establishment
of Biosafety Level-3 (BsaSL-3) laboratory: important criteria to consider while designing,
constructing, commissioning & operating the facility in Indian setting. The Indian journal of
medical research, 140(2), 171–183.
• Janosko, K., Holbrook, M. R., Adams, R., Barr, J., Bollinger, L., Newton, J. T., Ntiforo, C., Coe, L.,
Wada, J., Pusl, D., Jahrling, P. B., Kuhn, J. H., & Lackemeyer, M. G. (2016). Safety Precautions and
Operating Procedures in an (A)BSL-4 Laboratory: 1. Biosafety Level 4 Suit Laboratory Suite Entry
and Exit Procedures. Journal of visualized experiments: JoVE, (116),
52317. https://doi.org/10.3791/52317
• Standard Microbiological Practices for Biosafety Level 1 Laboratories at the University of
Tennessee-Knoxville, Institute of Agriculture and Graduate School of Medicine. January, 2013.
• Biosafety Manual. Boston University Research Support.
• https://www.criver.com/eureka/what-bsl-3-lab