BIOBARCODE AND NANOPARTICLE DETECTION
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This document discusses using nanotechnology to create DNA barcodes for identifying biological targets. It describes attaching unique DNA sequences to nanoparticles that can then bind to specific targets like proteins. Magnetic microparticles coated with antibodies first capture the target, and nanoparticle probes with complementary antibodies and DNA barcodes can then bind. The DNA barcodes are then detected using techniques like PCR to amplify the signal, allowing for ultrasensitive detection of targets down to the attomolar level. This "bio-barcode" approach using nanoparticles could provide a way to multiplex detection of different targets through the unique DNA sequences encoded on each nanoparticle probe.
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BIOBARCODE
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Application
Microarray database
Conclusion
Reference
Nucleic acid microarray
The above presentation consist of the definition of microarray, brief history, general principle of the same, the type of scanner that are used to read or to scan the microarray , type of DNA microarray and finally its various apliccation including the role of DNA microaarray in drug discovery.
Blotting techniques ppt
The document discusses various blotting techniques used to detect DNA, RNA, and proteins. It describes Southern blotting for detecting DNA, Northern blotting for detecting RNA, and Western blotting for detecting proteins. It provides details on the steps involved in each technique, including separating biomolecules by electrophoresis, transferring them to a membrane, and using probes for detection through hybridization or antibody binding.
Microarray
DNA microarrays contain thousands of DNA sequences attached to a solid surface in defined positions. Each DNA spot represents a single gene. The document describes the basic protocol for a DNA microarray experiment which involves isolating mRNA from samples, labeling the mRNA, hybridizing it to the microarray, and scanning the microarray to quantify gene expression levels. It also discusses various types of microarrays classified by their probes, such as cDNA, oligonucleotide, and SNP microarrays, as well as parameters that affect microarray fabrication.
DNA Barcoding and its application in species identification
1) The document introduces a seminar on DNA barcoding and its role in discriminating plant species, given by Kaldate Supriya.
2) DNA barcoding is a technique that uses short, standardized gene sequences from organisms as a genetic barcode for species identification and discrimination.
3) The most common plant barcoding markers are chloroplast genes matK and rbcL, which provide sufficient variability to discriminate most plant species.
Best Practices for Bioinformatics Pipelines for Molecular-Barcoded Targeted S...
Best Practices for Bioinformatics Pipelines for Molecular-Barcoded Targeted S...Genomika Diagnósticos
Poster Best Practices for Bioinformatics Pipelines for Molecular-Barcoded Targeted Sequencing
Authors: Marcel Caraciolo, Murilo Cervato, George Carvalho and Wilder Galvão.Molecular techniques in food microbiology
This document discusses molecular techniques that are used in food microbiology. It describes several applications of molecular methods including detecting genetically modified foods, food authenticity testing, and microbial contamination detection. Common molecular methods described are PCR, RFLP, PFGE, and DNA microarrays. Specific techniques covered in more depth include real-time PCR, multiplex PCR, plasmid profiling, ribotyping, AFLP, LAMP, FISH, and biosensors. The document provides details on the basic principles and procedures for several of these important molecular analysis methods.
Seminar
This document provides an overview of DNA microarrays. It begins with a brief introduction defining DNA microarrays and their use in analyzing gene expression. Next, it discusses the history and basic aspects of microarrays, including how oligonucleotides are coupled to a surface, sample preparation and hybridization, and scanning and data analysis. Applications of microarrays like gene expression analysis and limitations are also outlined. The document concludes with references used to compile the information presented.
[DSC Europe 23][DigiHealth] Branka Zukic Big Data Personalized medicine
The document discusses the development of a Pharmacogenomics Hub (PharmGenHUB) at the Institute of Molecular Genetics and Genetic Engineering (IMGGE) in Belgrade. The PharmGenHUB project aims to 1) sequence populations in the Western Balkan region to identify pharmacogenomic markers, 2) develop an electronic tool to provide personalized drug recommendations, and 3) discover novel pharmacogenomic markers using induced pluripotent stem cell models. The project involves collaboration between IMGGE and partners from universities and hospitals in the Western Balkan region and Europe.
Miarray:A new Technology For Life Science
Md. Abdul Momin presented on DNA microarray technology. Microarrays allow researchers to analyze gene expression levels of thousands of genes simultaneously using DNA probes attached to a solid surface. The presentation covered the history and principles of microarray technology, types of microarrays including glass cDNA and in situ oligonucleotide arrays, and applications such as disease diagnosis, drug discovery, and toxicology research. Microarrays are a powerful tool for functional genomics and comparative analysis across many fields of study.
DNA micro array by kk sahu
Introduction
Definition
History
Principle
Types
CDNA array
Oligonucleotide array
Microarray experiments
Microarray fabrication
Application
Microarray database
Conclusion
Reference
VAISHNAVI GHUGAL(SCREENING OF RECOMBINANTS).pptx
Screening of recombinants: blotting techniques, colony lift, immunological methods, south-western screening etc.
DNA microarray.pdfghghvjjsjsjdhdhdhddhdhdjdhd
DNA microarrays contain thousands of DNA probes attached to a solid surface that are used to simultaneously measure gene expression levels in a sample. The sample's DNA or RNA is extracted, labeled, and hybridized to the microarray to bind to complementary probes. A scanner then detects the bound material's fluorescence intensity to quantify gene expression levels. This allows researchers to analyze expression patterns and identify genes that are differently expressed under different conditions, such as disease states or drug treatments.
Poster 63 microbiologie
This study aimed to develop a sensitive method for detecting Mycobacterium tuberculosis DNA using molecular inversion probes (MIPs) combined with pyrosequencing readout. The researchers designed a MIP targeting a conserved 18 bp sequence in the DR locus of M. tuberculosis. Probing genomic DNA from 25 M. tuberculosis isolates with this MIP followed by pyrosequencing allowed detection of the target sequence in all strains. The method achieved a limit of detection of 500 fg of M. tuberculosis DNA and was able to detect the target even in the presence of contaminating human DNA.
Discovering drugs (I. Belda)
Jornada TOX® is a company that provides computational chemistry services for drug discovery, including receptor-based virtual screening, QSAR modeling, and macromolecular modeling, through their Intelligent Discovery, Intelligent Software, and Intelligent Knowledge business lines with offices in Barcelona, Heidelberg, and Cambridge. They have worked on over 100 research projects involving targets such as kinases and ion channels across therapeutic areas like oncology and cardiology using techniques like docking and molecular dynamics simulations. Jornada TOX® collaborates with academic partners and software companies and sells third party software for knowledge management in life sciences.
DNA sequencing by kk sahu sir
INTRODUCTION
DEFINITION
HISTORY
METHODS OF DNA SEQUENCING
MAXAM GILBERT METHOD
SANGERS METHOD
AUTOMATED DNA SEQUENCER
PYROSEQUENCING
SHOTGUN SEQUENCING
DNA MICROARRAY
APPLICATION
CONCLUSION
REFRENCES
3 - RT-qPCR.pdf
Participants of the workshop learn the necessary background information and techniques to diagnose Sars-CoV-2 using the mobile diagnostic laboratory. The laboratory is shipped ready to use with all devices, reagents, certificates, and protocols. After one day of preparation together with a local assistant, a five-day course is given where every step is carried out by each participant. Experts accompany the learning process with written teaching materials, video training, virtual live coaching, and short exams to verify the learned content.
DNA MICROARRAY
DNA microarrays allow researchers to study gene expression patterns across thousands of genes simultaneously. Microarrays work by hybridizing fluorescently-labeled cDNA or cRNA to complementary DNA probes affixed to a solid surface, such as a glass slide. There are two main types of microarrays: cDNA microarrays where cDNA fragments are spotted onto glass slides, and in situ synthesized oligonucleotide arrays with short DNA sequences directly built onto chips. Microarrays have numerous applications including gene expression profiling, comparative genomics, disease diagnosis, drug discovery, and toxicology research.
Gene Expression Analysis with microarray.pptx
Microarrays can genotype specific regions of a genome, identifying variations such as single nucleotide polymorphisms (SNPs).
Similar to BIOBARCODE AND NANOPARTICLE DETECTION (20)
DNA Barcoding and its application in species identification
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[DSC Europe 23][DigiHealth] Branka Zukic Big Data Personalized medicine
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Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...
The pygmy halfbeak Dermogenys colletei, is known for its viviparous nature, this presents an intriguing case of relatively low fecundity, raising questions about potential compensatory reproductive strategies employed by this species. Our study delves into the examination of fecundity and the Gonadosomatic Index (GSI) in the Pygmy Halfbeak, D. colletei (Meisner, 2001), an intriguing viviparous fish indigenous to Sarawak, Borneo. We hypothesize that the Pygmy halfbeak, D. colletei, may exhibit unique reproductive adaptations to offset its low fecundity, thus enhancing its survival and fitness. To address this, we conducted a comprehensive study utilizing 28 mature female specimens of D. colletei, carefully measuring fecundity and GSI to shed light on the reproductive adaptations of this species. Our findings reveal that D. colletei indeed exhibits low fecundity, with a mean of 16.76 ± 2.01, and a mean GSI of 12.83 ± 1.27, providing crucial insights into the reproductive mechanisms at play in this species. These results underscore the existence of unique reproductive strategies in D. colletei, enabling its adaptation and persistence in Borneo's diverse aquatic ecosystems, and call for further ecological research to elucidate these mechanisms. This study lends to a better understanding of viviparous fish in Borneo and contributes to the broader field of aquatic ecology, enhancing our knowledge of species adaptations to unique ecological challenges.
Equivariant neural networks and representation theory
Or: Beyond linear.
Abstract: Equivariant neural networks are neural networks that incorporate symmetries. The nonlinear activation functions in these networks result in interesting nonlinear equivariant maps between simple representations, and motivate the key player of this talk: piecewise linear representation theory.
Disclaimer: No one is perfect, so please mind that there might be mistakes and typos.
dtubbenhauer@gmail.com
Corrected slides: dtubbenhauer.com/talks.html
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Current descriptions of immersive learning cases are often difficult or impossible to compare. This is due to a myriad of different options on what details to include, which aspects are relevant, and on the descriptive approaches employed. Also, these aspects often combine very specific details with more general guidelines or indicate intents and rationales without clarifying their implementation. In this paper we provide a method to describe immersive learning cases that is structured to enable comparisons, yet flexible enough to allow researchers and practitioners to decide which aspects to include. This method leverages a taxonomy that classifies educational aspects at three levels (uses, practices, and strategies) and then utilizes two frameworks, the Immersive Learning Brain and the Immersion Cube, to enable a structured description and interpretation of immersive learning cases. The method is then demonstrated on a published immersive learning case on training for wind turbine maintenance using virtual reality. Applying the method results in a structured artifact, the Immersive Learning Case Sheet, that tags the case with its proximal uses, practices, and strategies, and refines the free text case description to ensure that matching details are included. This contribution is thus a case description method in support of future comparative research of immersive learning cases. We then discuss how the resulting description and interpretation can be leveraged to change immersion learning cases, by enriching them (considering low-effort changes or additions) or innovating (exploring more challenging avenues of transformation). The method holds significant promise to support better-grounded research in immersive learning.
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This is a short talk that I gave at the Banff International Research Station workshop on Modeling and Theory in Population Biology. The idea is to try to understand how the burden of natural selection relates to the amount of information that selection puts into the genome.
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The cost of information acquisition by natural selection
Ryan Seamus McGee, Olivia Kosterlitz, Artem Kaznatcheev, Benjamin Kerr, Carl T. Bergstrom
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BIOBARCODE AND NANOPARTICLE DETECTION
- 1. BIOBARCODE USINGBIOBARCODE USING NANOTECHNOLOGYNANOTECHNOLOGY DR. GURPREET SINGH, Ph.D, FSAB PG DEPARTMENT OF BIOTECHNOLOGY, LYALLPUR KHALSA COLLEGE, JALANDHAR PUNJAB EMAIL: drgurpreet@lkc.ac.in
- 2. BARCODEBARCODE: A barcode is an optical machine-readable representation of data, which shows data about the object to which it attaches. Originally, barcodes represented data by varying the widths and spacings of parallel lines, and may be referred to as linear or 1 dimensional (1D), rectangular or geomatrical shapes (2D). 3D barcode uses the height of the line to represent a product. 1D 2D 3D
- 3. DNA barcode • DNA barcoding is a taxonomic method that uses a short genetic marker in an organism's DNA to identify it as belonging to a particular species.
- 4. The basic concept of bar-code DNA. It carries with it a large number of oligonucleotides per protein binding event, there is substantial amplification and one can detect PSA at 30 attomolar concentration. Alternatively, one can do polymerase chain reaction on the oligonucleotide barcodes and boost the sensitivity to 3 attomolar.
- 9. TheDNA-BCAassay. Bio-Bar-Code-Based DNA Detection with PCR-like Sensitivity J. AM. CHEM. SOC. 2004, 126, 5932-5933
- 11. Ho et al., 2005. Multiplexed Hybridization Detection with Multi color Colocalization of Quantum Dot Nanoprobes. Nano Lett., Vol. 5, No. 9, 2005
- 12. Sequences of the Three Anthrax-Related Targets (rpoB, capC, and pagA) and Their Associated Oligonucleotide Probes.
- 13. Simulated multiplexed analysis of anthrax-related genetic targets for pathogenicity: (a) color pallet for the three pairs of targetspecific QD nanoprobes and their resulting colocalized fluorescent images upon sandwich hybridization; (b) four samples containing different combinations of the three targets, rpoB, capC, and pagA. Checks represent the existence of certain target sequences. Sample IV does not contain any target and is used as a negative control. (c) Fluorescent images I, II, III, and IV correlate with samples I, II, III, and IV, respectively.
- 15. Programmed Assembly of DNA-Functionalized Nanoparticles
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- 18. The system is based on magnetic microparticle probes with antibodies that specifically bind a target of interest [prostate specific antigen (PSA), amyloid beta oligomer] and nanoparticle probes that are encoded with DNA that is unique to the protein target of interest and antibodies that can sandwich the target captured by the microparticle probes . Magnetic separation of the complexed probes and target followed by dehybridization of the oligonucleotides on the nanoparticle probe surface allows one to determine the presence of the target protein by identifying the oligonucleotide sequence released from the nanoparticle probe Nanoparticle-Based Bio-Bar Codes for the Ultrasensitive Detection of Proteins
- 19. Nanoparticle-Based Bio-Bar Codes for the Ultrasensitive Detection of Proteins Nam et al., Science, 301, 1884-1886, 2003