This course offers an integrated examination of the major metabolic pathways in our bodies, focusing on carbohydrates, proteins, lipids, nucleic acids, and enzymes. It provides a comprehensive overview of the digestion, absorption, and metabolism of these biomolecules, along with an understanding of related disorders. The practical aspect of the course is designed to strengthen the theoretical knowledge through various biochemical experiments. Created by: Mr. Attuluri Vamsi Kumar, Assistant Professor, Department of MLT, UIAHS, Chandigarh University, Mohali, Punjab. For more details website: https://www.mltmaster.com

1. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Program Name: B.Sc. Medical Lab Technology
Syllabus of Biochemical metabolism
Course Name: Biochemical metabolism Course Type
Course Coordinator: Attuluri Vamsi Kumar – B.Sc. MLT, M.Sc. MLT, PhD (Perusing)
Designation: Assistant Professor
Department: Department of Medical Lab Technology (MLT)
Program Core
PRE-REQUISITE Basic Clinical Biochemistry Sem: 3 S. No: 21
About Vamsi: I am academician in Medical Laboratory Sciences with a strong desire to improve Outcome
based education (OBE) structured MLT education. I am constantly focusing on building an academic
atmosphere that is set high standards with strong multi blended teaching pedagogy models.
YouTube: https://www.youtube.com/@vamsiMLT
SlideShare: https://www.slideshare.net/VamsiIntellectual
Website: www.mltmaster.com / https://sites.google.com/view/vamsi-intellectual-protfolio/home
LinkedIn: https://www.linkedin.com/in/vamsi-kumar-attuluri-ab8987128/?originalSubdomain=in
Research Gate: https://www.researchgate.net/profile/Attuluri-Kumar
Orcid ID: https://orcid.org/0000-0001-9278-6714
Contact No: +91 7416660584
Mail ID: vamsifmlt@gmail.com
A. COURSE DESCRIPTION
This course offers an integrated examination of the major metabolic pathways in our bodies,
focusing on carbohydrates, proteins, lipids, nucleic acids, and enzymes. It provides a
comprehensive overview of the digestion, absorption, and metabolism of these biomolecules,
along with an understanding of related disorders. The practical aspect of the course is designed
to strengthen the theoretical knowledge through various biochemical experiments.
B. COURSE OBJECTIVES:
1. Recall the basic knowledge of biochemical metabolism, including the major metabolic pathways
of carbohydrates, proteins, lipids, nucleic acids, and enzymes.
2. Understand the process of digestion, absorption, and metabolism of these biomolecules.
3. Apply the theoretical knowledge in conducting biochemical experiments to determine the presence
and levels of these biomolecules.
4. Analyze the different metabolic disorders related to carbohydrates, proteins, lipids, and nucleic
acids.
5. Evaluate the impact of enzyme action on various metabolic processes, including the factors
affecting enzyme action and the implications of enzyme inhibitors.
C. COURSE OUTCOMES
CO
No
Statement Performance
Indicator
Level of
Learning
(Highest BT
Level)
Target
Attainment
CO1 Students will be able to recall and define the
fundamental concepts of biochemical metabolism
including the structures, functions, and
classifications of carbohydrates, proteins, lipids,
nucleic acids, and enzymes.
PI 2.5, PI 6 K1: Knowledge 70%
CO2 Students will understand the processes of digestion,
absorption, and metabolism of these biomolecules
and the roles they play in our bodies.
PI 2.1, PI 2.4 K2:
Comprehension
70%

2. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
CO3 Students will be able to apply their theoretical
knowledge to perform various biochemical
experiments, demonstrating competence in the use
of key lab techniques and protocols.
PI 2.2, PI 2.3,
PI 2.5, PI 2.6,
PI 2.7, PI 5, PI
7
K3: Application 70%
CO4 Students will be able to analyze and interpret
experimental data and understand the implications
of various metabolic disorders related to these
biomolecules.
PI 2.6, PI 2.8,
PI 3
K4: Analysis 70%
CO5 Students will be able to evaluate the factors affecting
enzyme actions, the implications of enzyme
inhibitors, and make judgements about the impact
of these on metabolic processes.
PI 2.8, PI 3, PI
4
K5: Evaluation 70%
CO6 Students will demonstrate the ability to integrate
their knowledge and understanding of biochemical
metabolism to devise new hypotheses or solutions
in the context of biochemical research.
PI 2.9, PI 6, PI
8
K6: Creation 70%
D. SYLLABUS
Unit-1 Introduction to Metabolism and Carbohydrates Contact Hours:15
Chapter 1.1 Overview of Biochemical Metabolism- Introduction, Importance, and Classification of
Biochemical Metabolism, Understanding Metabolic Pathways, Overview of Deficiency
Diseases
Chapter 1.2 Carbohydrate Metabolism- Introduction, Importance, and Classification of Carbohydrates,
Digestion and Absorption of Carbohydrates, Detailed Metabolism of Carbohydrates:
Glycolysis, Citric Acid Cycle, Gluconeogenesis, Glycogenolysis, Glycogenesis, Disorders of
Carbohydrate Metabolism
Experiment 1 /
Case study
To determine the presence of carbohydrates by Molisch test.
Experiment 2 /
Case study
To determine the presence of reducing sugar by Fehling solutions
Experiment 3 /
Case study
To determine the presence of reducing sugar by Benedicts method.
Unit-2 Proteins and Lipids Contact Hours:15
Chapter 2.1 Protein Metabolism - 21
Chapter 2.2 Lipid Metabolism- Introduction and Classification of Lipids, Digestion and Absorption of
Fats, Lipoproteins, Fatty Acid Biosynthesis and Fatty Acid Oxidation
Experiment 4 /
Case study
To determine starch by Iodine test.
Experiment 5 /
Case study
Determination of Glucose in serum & plasma
Experiment 6 /
Case study
Estimates of blood Glucose by Folin& Wu method
Experiment 7 /
Case study
Determination of Urea in serum, plasma & urine.
Unit-3 Nucleic Acids and Enzymes Contact Hours:15
Chapter 3.1 Nucleic Acid Metabolism- Introduction to Nucleic Acids, Functions of Nucleic Acids,
Functions of Energy Carriers
Chapter 3.2 Enzyme Metabolism- Introductions, Importance, and Classifications of Enzymes, Properties
of Enzymes, Mechanism of Enzyme Action, Factors Affecting Enzyme Action, Enzyme
Kinetics & Enzyme Inhibitors
Experiment 8 /
Case study
Determination of Creatinine in serum or plasma
Experiment 9 / Determination of serum Albuminq

3. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Case study
Experiment 10 /
Case study
Determination of Cholesterol in serum or plasma
Self-study topics for Advance learners: Advanced Carbohydrate Metabolism: Pentose
Phosphate Pathway, Sugar derivatives and their roles in cell signaling, metabolic control, and disease.,
Advanced Protein Metabolism: Protein folding and misfolding, Molecular chaperones, Post-
translational modifications, Proteomics., Advanced Lipid Metabolism: Role of lipids in cell signaling
and membrane dynamics, Eicosanoids and their role in inflammation and immunity, Sterol
metabolism.
E. TEXT BOOKS/REFERENCE BOOKS
TEXT BOOKS
T1 Practical Clinical Biochemistry by Harold Varley
T2 Textbook of Medical Laboratory Technology by P. B. Godker
T3 Medical Laboratory Technology by Mukherjee
REFERENCE BOOKS
R1 Principal of Biochemistry by M. A. Siddiqi
R2 Instrumental Analysis by Chatwal Anand
R3 Textbook of Medical Biochemistry by Chaterjee, Shinde

4. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Code Performance Indicators
PI 1 Demonstrate professional interpersonal, oral, and written communications skills sufficient to
serve the needs of patients and the public including an awareness of how diversity may affect
the communication process.
PI 2 Perform pre-analytical, analytical, and post-analytical processes:
PI 2.1 Demonstrate ability to understand investigation/test requisition.
PI 2.2 Collecting the relevant clinical samples along with complete and accurate documentation with
proper safety measures in relation to sample accountability.
PI 2.3 To transport the samples with precautionary measures to the relevant lab section.
PI 2.4 Demonstrate the ability to prepare clinical sample for processing
PI 2.5 To demonstrate the knowledge of accurate sample processing for the required lab
investigation. Perform routine clinical laboratory tests in clinical chemistry,
haematology/haemostasis, immunology, immunohematology, microbiology, Histopathology,
Cytopathology, body fluid analysis, and laboratory operations.
PI 2.6 Perform mathematical calculations related to all areas of the clinical laboratory
PI 2.7 Ability to record the test results/data.
PI 2.8 To demonstrate the ability to interpret the test reports and its documentation in lab records.
PI 2.9 Demonstrate ability to release the report to the right person in minimum turn-around time
(TAT).
PI 3 Perform problem solving and troubleshooting techniques for laboratory methodologies
Correlate laboratory test results with patient diagnosis and treatment.
PI 4 To follow basic quality assessment protocol of clinical laboratory.
PI 5 Demonstrate routine laboratory techniques sufficient to orient new employees within the
clinical laboratory.
PI 6 Apply basic scientific principles in learning new techniques/procedures; demonstrate
application of principles and methodologies.
PI 7 Utilize computer technology applications to interact with computerized instruments and
laboratory information systems.
PI 8 Demonstrate adequate knowledge of computer software as it applies to document production,
spreadsheets, and presentations.
PI 9 Demonstrate professional behaviour with co-team mates.
PI 10 Demonstrate sensitivity and compassion towards patients.