Vaccine discovery
DNA vaccine, mechanism
methods of delivery
Main concerns: do DNA vaccines cause insertional mutation and elicit anti-DNA antibodies in the body?
completed and ongoing trials.
new strategies: Prime Boost vaccines
Future prospects
Vaccine discovery
DNA vaccine, mechanism
methods of delivery
Main concerns: do DNA vaccines cause insertional mutation and elicit anti-DNA antibodies in the body?
completed and ongoing trials.
new strategies: Prime Boost vaccines
Future prospects
DNA vaccines (types, method and mechanism) Aneela Rafiq
DNA Vaccine is very promising method in current century. it can eliminate the risks of encountering pathogen with living cell.
this presentation has a brief concept about DNA Vaccine, to understand the baseline of genetic vaccine.
As a pioneer in the field of antibody research and development, Creative Biolabs has successfully applied our special DNA immunization service for hundreds of antibody discovery projects. Whether the interested antigen is a membrane protein or other challenging protein, our experienced scientists are confident in tailoring the most appropriate protocols of DNA immunization approach to meet our customers’ specific requirements.
This presentation talks about vaccines, currently being used in medicinal processes and therapeutics and their types. It elaborates the importance of the different types of vaccines along with their examples and their mechanism of action. The mode of production of all the types of vaccines is also discussed in the presentation including recent developments made for the production of mRNA vaccine against SARS-CoV-2
Watch the slideshow for a better understanding: https://youtu.be/frmGwCEtDnM
1. Learn how mRNA vaccines work.
2. Learn challenges in making HIV vaccines.
3. Learn about the advantage of mRNA vaccines in HIV
4. Learn about trials.
mRNA vaccine is a novel vaccine technology, which delivers mRNA that encoding the antigen protein of pathogen to the cell, and expresses the antigen protein, and then stimulates the immune response of the body.
Creative Biolabs has developed non-replicating mRNA vaccine platform, mRNA vaccine platform, mRNA pharmacology optimization platform, and and Self-amplifying mRNA vaccine platform to spport your vaccine researches. If you need more information about mRNA vaccine, please follow us.
The Production of Vaccines using Genetic Engineering as the world’s population continues to rise annually, new technology becomes known to man! Technology is a never-ending process where newer and better things are being discovered. The area of technology that will be discussed here is biotechnology. Biotechnology is the harnessing by man of the ability of organisms to produce drugs, food or other useful products. Micro-organisms are the main ones involved in biotechnology, especially bacteria and fungi. More recently, genetic engineering or the altering of the genes, the building blocks which determine the make-up of an organism, has been increasingly used in biotechnology.
This presentation focuses on the science of Gene Therapy, the techniques of germ-line and somatic gene therapy and the mechanism of curing diseases and disorders using gene therapy. The presentation starts by discussing some common basic terms from genetics and moves on to the historical development of gene therapy techniques in chronological order. The different types of gene therapy techniques and their mechanisms have been discussed in detail subsequently. In concluding slides, some commercially available gene therapy products are mentioned and challenges of gene-therapy techniques have been highlighted.
DNA vaccines (types, method and mechanism) Aneela Rafiq
DNA Vaccine is very promising method in current century. it can eliminate the risks of encountering pathogen with living cell.
this presentation has a brief concept about DNA Vaccine, to understand the baseline of genetic vaccine.
As a pioneer in the field of antibody research and development, Creative Biolabs has successfully applied our special DNA immunization service for hundreds of antibody discovery projects. Whether the interested antigen is a membrane protein or other challenging protein, our experienced scientists are confident in tailoring the most appropriate protocols of DNA immunization approach to meet our customers’ specific requirements.
This presentation talks about vaccines, currently being used in medicinal processes and therapeutics and their types. It elaborates the importance of the different types of vaccines along with their examples and their mechanism of action. The mode of production of all the types of vaccines is also discussed in the presentation including recent developments made for the production of mRNA vaccine against SARS-CoV-2
Watch the slideshow for a better understanding: https://youtu.be/frmGwCEtDnM
1. Learn how mRNA vaccines work.
2. Learn challenges in making HIV vaccines.
3. Learn about the advantage of mRNA vaccines in HIV
4. Learn about trials.
mRNA vaccine is a novel vaccine technology, which delivers mRNA that encoding the antigen protein of pathogen to the cell, and expresses the antigen protein, and then stimulates the immune response of the body.
Creative Biolabs has developed non-replicating mRNA vaccine platform, mRNA vaccine platform, mRNA pharmacology optimization platform, and and Self-amplifying mRNA vaccine platform to spport your vaccine researches. If you need more information about mRNA vaccine, please follow us.
The Production of Vaccines using Genetic Engineering as the world’s population continues to rise annually, new technology becomes known to man! Technology is a never-ending process where newer and better things are being discovered. The area of technology that will be discussed here is biotechnology. Biotechnology is the harnessing by man of the ability of organisms to produce drugs, food or other useful products. Micro-organisms are the main ones involved in biotechnology, especially bacteria and fungi. More recently, genetic engineering or the altering of the genes, the building blocks which determine the make-up of an organism, has been increasingly used in biotechnology.
This presentation focuses on the science of Gene Therapy, the techniques of germ-line and somatic gene therapy and the mechanism of curing diseases and disorders using gene therapy. The presentation starts by discussing some common basic terms from genetics and moves on to the historical development of gene therapy techniques in chronological order. The different types of gene therapy techniques and their mechanisms have been discussed in detail subsequently. In concluding slides, some commercially available gene therapy products are mentioned and challenges of gene-therapy techniques have been highlighted.
A DNA vaccine is a type of vaccine that transfects a specific antigen-coding DNA sequence into the cells of an organism as a mechanism to induce an immune response.
DNA vaccines work by injecting genetically engineered plasmid containing the DNA sequence encoding the antigen(s) against which an immune response is sought, so the cells directly produce the antigen, thus causing a protective immunological response.
vaccine is a biological preparation that provides active acquired immunity to a particular disease. A vaccine typically contains an agent that resembles a disease-causing microorganism and is often made from weakened or killed forms of the microbe, its toxins, or one of its surface proteins. The agent stimulates the body's immune system to recognize the agent as a threat, destroy it, and to further recognize and destroy any of the microorganisms associated with that agent that it may encounter in the future.
HISTORY OF VACCINES-
EDWARD JENNER conduct experiments in 1796 that lead to the creation of the first smallpox vaccine for prevention of smallpox.
A vaccine for RABIES is developed by LOUIS PASTEUR .
Vaccine for COLERA and TYPHOID were developed in 1896 and PLAGE vaccine in 1887.
The first DIPHTHERIA vaccine is developed in about 1913 by EMIL ADOLPH BEHRING,WILLIAM HALLOCK PARK.
The whole cell PERTUSIS vaccines are developed in 1914.
A TETANUS vaccine is developed in 1927.
vaccine train user immune system to create antibodies, just as it when it is exposed to a disease. However, because vaccine contain only killed or weakened forms of germs like viruses or bacteria, they do not cause the disease or put you at the risk of complications.
vaccine is a biological preparation that improve immunity to a particular disease.
A vaccine typically contain an agent that resembles a disease causing microorganisms and is often made from weakened or killed forms of the microbes.
Immunity: Protection from an infectious disease. If you are immune to a disease, you can be exposed to it without becoming infected.
Vaccine: A preparation that is used to stimulate the body’s immune response against diseases. Vaccines are usually administered through needle injections, but some can be administered by mouth or sprayed into the nose.
Vaccination: The act of introducing a vaccine into the body to produce protection from a specific disease.
Replication,transcription,translation complete the central dogma of life.How mRNA,tRNA,rRNA act on ribosomes for protein synthesis.Difference between eukaryotes and prokaryotes
2. CONTENTSCONTENTS
Introduction
History
DNA vaccines Vs Traditional
vaccines
How DNA vaccine is made
Methods of delivery
How DNA vaccine works
Advantages
Disadvantages
Current clinical trials
Safety issues
Future of DNA vaccines
Conclusion
References
3. INTRODUCTIONINTRODUCTION
DNA vaccine is DNA sequence used as a vaccine.
This DNA Sequence code for antigenic protein of
pathogen.
As this DNA inserted into cells it is translated to form
antigenic protein. As this protein is foreign to cells ,
so immune response raised against this protein.
In this way ,DNA vaccine provide immunity against
that pathogen.
4. HISTORYHISTORY
In 1990, University of Wisconsin, Jon Wolff found that
injection of DNA plasmids produce a protein response
in mice.
In 1993, Merck Research Laboratories, Dr. Margaret
Liu found that intramuscular injection of DNA from
influenzae virus into mice produced complete
immune response
In 1996, trials involving T-cell lymphoma, influenzae &
herpes simplex virus were started
5. DNA vaccines Vs Traditional vaccinesDNA vaccines Vs Traditional vaccines
Uses only the DNA from
infectious organisms.
Avoid the risk of using
actual infectious
organism.
Provide both Humoral &
Cell mediated immunity
Refrigeration is not
required
Uses weakened or killed
form of infectious
organism.
Create possible risk of the
vaccine being fatal.
Provide primarily Humoral
immunity
Usually requires
Refrigeration.
DNA vaccines Traditional vaccines
6. HOW DNA VACCINE IS MADEHOW DNA VACCINE IS MADE
Viral gene
Expression
plasmid
Plasmid with foreign gene
Recombinant DNA
Technology
12. HOW DNA VACCINE WORKSHOW DNA VACCINE WORKS
BY TWO PATHWAYS
ENDOGENOUS :- Antigenic Protein is presented by
cell in which it is produced
EXOGENOUS :- Antigenic Protein is formed in
one cell but presented by
different cell
18. WHEN VIRUS ENTER IN THEWHEN VIRUS ENTER IN THE
BODYBODY
Viral Protein
Memory T-Cell
Antibodies
19. ADVANTAGESADVANTAGES
Elicit both Humoral & cell mediated
immunity
Focused on Antigen of interest
Long term immunity
Refrigeration is not required
Stable for storage
21. CURRENT CLINICAL TRIALSCURRENT CLINICAL TRIALS
June 2006,DNA vaccine examined on horse
Horse acquired immunity against west
nile viruses
August 2007,DNA vaccination against multiple
Sclerosis was reported as being effective
27. FUTURE PROSPECTSFUTURE PROSPECTS
Plasmid with multiple genes provide immunity against
many diseases in one booster
DNA vaccines against infectious diseases such as AIDS,
Rabies, Malaria can be available
28. CONCLUSIONCONCLUSION
DNA vaccines are in their early phase.
There are no DNA vaccines in market at
present.
But this just the beginning .
DNA vaccines are going to be the vaccines of
next generation.
