This document describes the development of a prototype multiplex lateral flow device (LFD) that can simultaneously detect foot-and-mouth disease virus (FMDV) and identify its serotype (O, A, or Asia1). The test takes only a few minutes without any specialized equipment. Performance of the multiplex LFD was comparable to antigen-ELISA testing. Further validation is underway in endemic areas, along with developing an LFD to identify SAT1 and SAT2 FMDV serotypes.

1. 1EuFMD | Open Session special edition | #OS20se
Santina GRAZIOLI
Emiliana BROCCHI
Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna (IZSLER), Brescia, Italy
A MULTIPLEX LATERAL FLOW DEVICE FOR ON-FIELD
IDENTIFICATION AND SEROTYPING OF
FOOT-AND-MOUTH DISEASE VIRUS

2. 2EuFMD | Open Session special edition | #OS20se
 The LFD is suited for timely ON-SITE FMD DIAGNOSIS
 We developed a prototype multiplex LFD for FMDV
detection (pan-FMDV) and simultaneous serotyping of
FMDV O, A, Asia1 in a single strip
 The test takes few minutes without requiring any
equipment
OUTPUT
 The performances of the Antigen-ELISA and the multiplex
LFD are comparable, as the same catching and detector
monoclonal antibodies are used in both kits.
MULTIPLEX LFD = MULTIPLEXING CAPABILITY AND MAXIMUM SIMPLICITY
Reaction in line 1 and 4 = FMDV Positive type A
WORK IN PROGRESS …
Additional validation of the technology in endemic areas
Studies to develop a multiplex LFD for typing of SAT1 and SAT2 FMD viruses (FMDV Ag Device 2)

3. 3EuFMD | Open Session special edition | #OS20se
Santina Grazioli
Emiliana Brocchi
Efrem Foglia Giulia Pezzoni
Laura Anfossi Simone Cavalera
Sergio Rosati
Chiara Nogarol

4. eufmdvirtual.com
8, 10, 15, 17 December 2020
EuFMD / Open Session