OS20 Online 15 December

1. 1EuFMD | Open Session special edition | #OS20se
National Animal and Plant Quarantine Agency / Republic of Korea
Development of Solid-phase Competition
ELISA for detection of type-A Foot and
Mouth Disease Virus Antibodies
YouJin Han (Presenter), Doheon Kwon, JiHyun Lee, Hyun Mi Pyo,
Eun-Jin Choi, Wonseok Shin, JaeMyoung Kim and Mi-Young Park

2. 2EuFMD | Open Session special edition | #OS20se
 The new solid-phase blocking ELISA Type-A (SPBE) for detection of
SP antibodies to FMDv type-A was validated using test panels of sera
from naïve, vaccinated, and from vaccinated-and sequentially
infected animals, and international reference sera FAO phaseⅩⅧ,
IAEA FMD Control sera
 The recombinant protein (rP13C) was derived from the P1 precursor
and 3C protease genes from FMDv A/SKR/2018 P13C using a
baculovirus-expressed system.
 The competitor monoclonal antibody (mAb) for the ELISA was
generated by immunizing a VP1 peptide corresponding to the GH
loop for high correlation of FMDV neutralizing antibodies.

3. 3EuFMD | Open Session special edition | #OS20se
 When test sera at a 1:4 dilution with a cut-off point of 0.7 S/N ratio of
reaction for bovine and 0.6 S/N ratio for pigs, the performance of
solid-phase competition ELISA showed the highest concordance rate
with that of VNT for serum panel obtained from infected or vaccinated
animals.
 A high level of type-specificity was demonstrated examining sera
strongly positive against other FMDv serotypes.
 The sensitivity and specificity of the SPB-ELISA type-A relative to the
VNT were 90.9% and 98.3% respectively, and high agreement
(concordance = 92.4%) was observed between two tests.

4. 4EuFMD | Open Session special edition | #OS20se
 Objectives
A recombinant-protein based ELISA has been developed for the
measurement of antibodies to FMDV serotype A and has been validated
using a wide range of sera from cattle and pigs to assess the adoption
for national FMD sero-surveillance
 Outcomes
The result of this study indicates the SPC-ELISA is simple and robust
serological test tool that can replace the VNT for screening SP
antibodies against FMDV type-A.

5. eufmdvirtual.com
8, 10, 15, 17 December 2020
EuFMD / Open Session