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OS20 - Developing of a liquid-phase blocking ELISA based on Foot-and-Mouth disease virus A/YEONCHEON/2017 for post-vaccination sero-monitoring - Seung Heon Lee

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EuFMD
EuFMD

OS20 Online 15 December

Health & Medicine
1 of 4
1EuFMD | Open Session special edition | #OS20se SEUNG HEON LEE Center for FMD Vaccine Research, Animal and Plant Quarantine Agency, Republic of Korea DEVELOPMENT OF A LIQUID-PHASE BLOCKING ELISA BASED ON FOOT-AND- MOUTH DISEASE VIRUS A/YEONCHEON/2017 FOR POST- VACCINATION SERO-MONITORING
2EuFMD | Open Session special edition | #OS20se • Development of a LPB-ELISA for measuring the level of antibodies against FMDV A/Yeoncheon/SKR/2017 (Sea- 97/G2 lineage) in sera from vaccinated animals MAIN IDEAS • Validation of diagnostic performance of the developed LPB-ELISA using an extensive range of sera from pigs
3EuFMD | Open Session special edition | #OS20se • When porcine sera (n=133) at a 1:10 dilution with a cut-off point of 50% inhibition of reaction, LPB-ELISA exhibited proper analytic performance with a high diagnostic sensitivity (92.1%) and specificity (88.6%) in comparison with the VNT OUTCOMES
eufmdvirtual.com 8, 10, 15, 17 December 2020 EuFMD / Open Session

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OS20 - Developing of a liquid-phase blocking ELISA based on Foot-and-Mouth disease virus A/YEONCHEON/2017 for post-vaccination sero-monitoring - Seung Heon Lee

  • 1. 1EuFMD | Open Session special edition | #OS20se SEUNG HEON LEE Center for FMD Vaccine Research, Animal and Plant Quarantine Agency, Republic of Korea DEVELOPMENT OF A LIQUID-PHASE BLOCKING ELISA BASED ON FOOT-AND- MOUTH DISEASE VIRUS A/YEONCHEON/2017 FOR POST- VACCINATION SERO-MONITORING
  • 2. 2EuFMD | Open Session special edition | #OS20se • Development of a LPB-ELISA for measuring the level of antibodies against FMDV A/Yeoncheon/SKR/2017 (Sea- 97/G2 lineage) in sera from vaccinated animals MAIN IDEAS • Validation of diagnostic performance of the developed LPB-ELISA using an extensive range of sera from pigs
  • 3. 3EuFMD | Open Session special edition | #OS20se • When porcine sera (n=133) at a 1:10 dilution with a cut-off point of 50% inhibition of reaction, LPB-ELISA exhibited proper analytic performance with a high diagnostic sensitivity (92.1%) and specificity (88.6%) in comparison with the VNT OUTCOMES
  • 4. eufmdvirtual.com 8, 10, 15, 17 December 2020 EuFMD / Open Session