This document discusses ANO cell stains, which can stain cell walls, membranes, and nuclei for use under microscopes. The stains are food-grade safe and photostable for at least 1 hour under various microscope and laser devices. Examples are provided of ANO stains being used to visualize stem hair cell walls, onion root tip cells at different mitosis stages, xylem and phloem in dicot stem sections, hibiscus epidermal cells and stomata, dicot stem pith cells, and human buccal epithelial cells. The document also mentions live/dead cell tests, live cell imaging, FACS applications, and partners and licensing information for ANO cell stains