The newly developed APQA ELISA kit for detecting FMDV showed better efficacy than the OIE reference Pirbright ELISA kit. The APQA kit was tested on virus samples isolated from FMD outbreaks in Vietnam, Laos, Myanmar, and Cambodia between 2015-2019. The APQA kit demonstrated higher sensitivity in detecting O type FMDV samples and was quicker and simpler than the Pirbright kit, completing the testing in around 2 hours less time. In conclusion, the new APQA ELISA kit is expected to significantly contribute to FMD control and prevention efforts in the Pool 1 region by providing enhanced diagnosis of the virus.

1. 1EuFMD | Open Session special edition | #OS20se
ENHANCED DIAGNOSIS EFFICACY OF A
NEWLY DEVELOPLED
ELISA KIT FOR FMDV IN POOL 1 REGION
Animal and Plant Quarantine Agency, Foot-and-mouth disease division /
Republic of Korea.
BooMi La

2. 2
FMDV have been circulating in Southeast Asia
such as Vietnam, Laos, Myanmar, Thailand for a
long time, and viral genetic substitutions would
be accumulated by selection pressure of
transmission across hosts and immunity induced
by infection or vaccination. It is important to
collect and analyze the same genotype virus that
has already occurred in Southeast Asia in order
to cope with FMD in Korea in the near future.
So in this study, FMDVs were isolated from clinical samples of FMD
outbreaks in 2015-2019 of Vietnam (n=9), Laos (n=12), Myanmar
(n=1), and Cambodia (n=30) through the international collaborative
research projects and subjected to performance comparison
between OIE reference ELISA kit (Pirbright kit) and APQA ELISA kit
newly developed from our laboratory.

3. 3EuFMD | Open Session special edition | #OS20se

4. 4
Figure 2. Results of APQA and Pirbright Ag ELISA .
In most O type FMDV samples, APQA Ag ELISA showed better sensitivity than Pirbright Ag
ELISA. In the case of A type FMDV sample, APQA was better in Laos(F), but Pirbright,
which has two Mab strips, showed higher sensitivity in the second Mab strip in Cambodia
samples(B). Meanwhile, non-specific A strip(B) in Pirbright ELISA were observed positive
for O type samples.
The APQA ELISA kit had better sensitivity and
specificity than Pirbright ELISA kit. Besides, the
APQA ELISA kit is quick and simple by saving
approximately 2hrs, being compared with the
Pirbright ELISA kit. Conclusively, the APQA
ELISA kit are expected to make a significant
contribution to control and prevention of FMD
in POOL 1 region.
Conclusion

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8, 10, 15, 17 December 2020
EuFMD / Open Session