Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.
1
INSTRUMENTALANALYSIS
LAB.
Mr Haydar A.M.S
BSc, MSc
Faculty of Science
Chemistry Department
Email: Haydar.kovly@gmail.com
2
Flame Atomic Absorption
Spectroscopy
Lab 9
Aim of the experiment
3
 To be familiar with using flame atomic absorption spectroscopy.
 Determination of calcium (Ca) ...
4
Atomic Absorption Spectroscopy
Atomic absorption spectrometry (AAS) is an analytical technique that
measures the concent...
5
The AAS instrument
Atomic Absorption Spectroscopy
Flame Atomic Absorption Spectroscopy:
6
The technique requires a liquid sample to be aspirated, aerosolized, and mixed wit...
Flame Atomic Absorption Spectroscopy:
•When metals are exposed to heat, they absorb light
that emit from the source.
•Each...
1. We set the instrument
at certain wavelength
suitable for a certain
element 2. The element in the
sample will be atomize...
Light absorption
9
unexcited ground state atoms, which absorb light at characteristic wavelengths,
as shown in the figure....
Lamp (Hollow Cathode Lamp)
10
Consists of a cathode and an anode. The
cathode is made of the element of interest.
The Anod...
Lamp (Hollow Cathode Lamp)
11
4- When these excited atoms decay to lower energy
levels they emit a few spectral lines char...
Lamp (Hollow Cathode Lamp)
12
Atomic Absorption Spectroscopy
13
Fuel and oxidant
flame
b Air – acetylene
Air- propane
Air- hydrogen
b Nitrous oxide – acetylene
Auxiliary
oxidant
Fuel
...
14
Common fuels and oxidants used in flame spectroscopy
Atomic Absorption Spectroscopy
15
The Flame
Calcium Flame Copper Flame Potassium Flame Manganese Flame
Atomic Absorption Spectroscopy
16
Atomic Absorption Spectroscopy
PerkinElmer Analyst 200
17
Atomic Absorption Spectroscopy
Lamp installation
18
Atomic Absorption Spectroscopy
Nebulizer
To convert the test solution to
gaseous atoms (produce a mist or
aerosol)
19
Atomic Absorption Spectroscopy
Safety Consideration
20
Atomic Absorption Spectroscopy
Safety Consideration
21
Atomic Absorption Spectroscopy
Safety Consideration
Dealing carefully with gas cylinder
and air compressor.
Upcoming SlideShare
Loading in …5
×

Flame Atomic Absorption Spectroscopy

Flame Atomic Absorption Spectroscopy

  • Be the first to comment

Flame Atomic Absorption Spectroscopy

  1. 1. 1 INSTRUMENTALANALYSIS LAB. Mr Haydar A.M.S BSc, MSc Faculty of Science Chemistry Department Email: Haydar.kovly@gmail.com
  2. 2. 2 Flame Atomic Absorption Spectroscopy Lab 9
  3. 3. Aim of the experiment 3  To be familiar with using flame atomic absorption spectroscopy.  Determination of calcium (Ca) in bottled water.
  4. 4. 4 Atomic Absorption Spectroscopy Atomic absorption spectrometry (AAS) is an analytical technique that measures the concentrations of elements. • Used for qualitative and quantitative detection. • It’s used for the determination of the presence and concentrations of metals in liquid samples. • Metals that can be detected include Fe, Cu, Al, Pb, Ca, Zn, Cd and many more. • Concentrations range is in the low mg/L (ppm) range.
  5. 5. 5 The AAS instrument Atomic Absorption Spectroscopy
  6. 6. Flame Atomic Absorption Spectroscopy: 6 The technique requires a liquid sample to be aspirated, aerosolized, and mixed with combustible gases, such as acetylene and air or acetylene and nitrous oxide. The mixture is ignited in a flame whose temperature ranges from 2100 to 2800 ºC. Atomic Absorption Spectroscopy
  7. 7. Flame Atomic Absorption Spectroscopy: •When metals are exposed to heat, they absorb light that emit from the source. •Each metal absorbs light at a characteristic frequency. For example: Metal Zn Fe Cu Ca Na λ (nm) 214 248 325 423 589 Atomic Absorption Spectroscopy
  8. 8. 1. We set the instrument at certain wavelength suitable for a certain element 2. The element in the sample will be atomized by heat 4. The monochromator isolates the line of interest 3. The element in the sample will absorb some of the light, thus reducing its intensity 5. The detector measures the change in intensity 6. A computer data system converts the change in intensity into an absorbance The simple diagram for the AAS Atomic Absorption Spectroscopy
  9. 9. Light absorption 9 unexcited ground state atoms, which absorb light at characteristic wavelengths, as shown in the figure. Atomic Absorption Spectroscopy
  10. 10. Lamp (Hollow Cathode Lamp) 10 Consists of a cathode and an anode. The cathode is made of the element of interest. The Anode consists of tungsten 1. A large voltage across the anode and cathode will cause the inert gas to ionize. 2. The inert gas ions will then be accelerated into the cathode, sputtering off atoms from the cathode. 3. Both the inert gas and the sputtered cathode atoms will in turn be excited by collisions with each other. Atomic Absorption Spectroscopy
  11. 11. Lamp (Hollow Cathode Lamp) 11 4- When these excited atoms decay to lower energy levels they emit a few spectral lines characteristic of the element of interest. 5- The light is emitted directionally through the lamp's window, a window made of a glass transparent in the UV and visible wavelengths. 6- The light can then be detected and a spectrum can be determined. Atomic Absorption Spectroscopy
  12. 12. Lamp (Hollow Cathode Lamp) 12 Atomic Absorption Spectroscopy
  13. 13. 13 Fuel and oxidant flame b Air – acetylene Air- propane Air- hydrogen b Nitrous oxide – acetylene Auxiliary oxidant Fuel Atomic Absorption Spectroscopy
  14. 14. 14 Common fuels and oxidants used in flame spectroscopy Atomic Absorption Spectroscopy
  15. 15. 15 The Flame Calcium Flame Copper Flame Potassium Flame Manganese Flame Atomic Absorption Spectroscopy
  16. 16. 16 Atomic Absorption Spectroscopy PerkinElmer Analyst 200
  17. 17. 17 Atomic Absorption Spectroscopy Lamp installation
  18. 18. 18 Atomic Absorption Spectroscopy Nebulizer To convert the test solution to gaseous atoms (produce a mist or aerosol)
  19. 19. 19 Atomic Absorption Spectroscopy Safety Consideration
  20. 20. 20 Atomic Absorption Spectroscopy Safety Consideration
  21. 21. 21 Atomic Absorption Spectroscopy Safety Consideration Dealing carefully with gas cylinder and air compressor.

    Be the first to comment

    Login to see the comments

  • ShwetaMishra115

    Sep. 30, 2018
  • AbassAlshamary1

    Dec. 17, 2018
  • BinduKundu

    Jan. 4, 2019
  • APURVASONI6

    Mar. 29, 2019
  • judyjerry

    May. 21, 2019
  • israahuseein

    Jun. 29, 2019
  • RozhanHNamiq

    Aug. 25, 2019
  • IolaRodrigues

    Nov. 29, 2019
  • ArapMulilo

    Dec. 3, 2019
  • PreetiSingh97

    May. 5, 2020
  • PritiKarasariya

    Oct. 25, 2020
  • DeepikaStalin2

    Nov. 5, 2020
  • FatiTorab

    Dec. 12, 2020
  • ShaikhMoin7

    Feb. 28, 2021
  • ShibanandaHalder

    Mar. 1, 2021
  • TahirMohammed9

    Mar. 16, 2021
  • ASHUTOSHKUMARSINGH38

    Apr. 15, 2021
  • PardeepKori

    May. 30, 2021
  • dimplethakur11

    Jun. 1, 2021
  • LismaHappy

    Jul. 19, 2021

Flame Atomic Absorption Spectroscopy

Views

Total views

16,125

On Slideshare

0

From embeds

0

Number of embeds

30

Actions

Downloads

395

Shares

0

Comments

0

Likes

36

×