The document provides an overview of key concepts in proteomics, including: 1) It discusses protein structure and function, the 20 common amino acids, and post-translational modifications that proteins undergo. 2) It introduces common techniques used in proteomics like chromatography, electrophoresis, mass spectrometry, and bioinformatics. 3) It summarizes protein analysis methods like gel electrophoresis, isoelectric focusing, and immunological assays used to detect and purify proteins of interest.

1. Proteins – Basics you need to know for Proteomics

5. Aliphatic side chains (hydrophobic)

9. Disulfide Bonds - Cys Reduced Oxidized Remember: LEO says GER

22. Tunnel for growing polypeptide chain

25. Why Formylation of Met? Termination of translation if not formylated!

27. Gel Filtration – molecular size

28. Ion exchange chromatography + + + + Cl - Cl - Cl - Cl - + + + + + + + + Cl - Cl - Cl - Cl -

29. Affinity Chromatography Equilibrate column Load feed onto column Then wash contaminants away Elute Product Clean Column and re-equilibrate column

38. IEF Examples Monoclonal Antibody Production

43. IgG’s

44. Ag/Ab interactions

47. ELISAs

48. Western Blots

49. Immunohistochemistry, Immunoelectron microscopy