Histology Procedure

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Histology Procedure informs the user about different steps involved in preparing a tissue for examination

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Histology Procedure

  1. 1. HISTOLOGY PROCEDURE<br />
  2. 2.
  3. 3. HISTOLOGY PROCEDURE<br />SPECIMEN ACCESSIONING<br />TISSUE FIXATION<br />GROSS EXAMINATION<br />TISSUE PROCESSING<br />DEHYDRATION<br />CLEARING<br />IMPREGNATION <br />TISSUE EMBEDDING<br />SLIDE STAINING<br />TISSUE SECTIONING<br />
  4. 4. HISTOLOGY PROCEDURE<br />SPECIMEN ACCESSIONING<br />
  5. 5. SPECIMEN ACCESSIONING<br />Specimens are accessioned by giving them a number that will identify each specimen for each patient<br />
  6. 6. HISTOLOGY PROCEDURE<br />SPECIMEN ACCESSIONING<br />GROSS EXAMINATION<br />
  7. 7. GROSS EXAMINATION<br /> Consists of describing the specimen and placing all or parts of it into a small plastic cassette<br />
  8. 8. HISTOLOGY PROCEDURE<br />SPECIMEN ACCESSIONING<br />TISSUE FIXATION<br />GROSS EXAMINATION<br />
  9. 9. TISSUE FIXATION<br /> The objective of fixation is to preserve cells and tissue constituents in as close a life-like state as possible<br />
  10. 10. TISSUE FIXATION<br /> Fixation coagulates Tissue proteins and constituents, a necessary event to prevent their loss or diffusion during tissue processing<br />
  11. 11. HISTOLOGY PROCEDURE<br />SPECIMEN ACCESSIONING<br />TISSUE FIXATION<br />GROSS EXAMINATION<br />TISSUE PROCESSING<br />DEHYDRATION<br />CLEARING<br />IMPREGNATION <br />
  12. 12. TISSUE PROCESSING<br /> The objective is to embed the tissue in a solid medium firm enough to support the tissue and give it sufficient rigidity to enable thin sections to be cut<br />
  13. 13. TISSUE PROCESSING<br />DEHYDRATION : Removal of fixative and water from the Tissue and replacing it with dehydrating fluid<br />CLEARING: Replacement of dehydrating fluid with a fluid that is totally miscible with both the dehydrating fluid and the embedding medium<br />IMPREGNATION: Replacement of clearing agent with the embedding medium<br />
  14. 14. HISTOLOGY PROCEDURE<br />SPECIMEN ACCESSIONING<br />TISSUE FIXATION<br />GROSS EXAMINATION<br />TISSUE PROCESSING<br />DEHYDRATION<br />CLEARING<br />IMPREGNATION <br />TISSUE EMBEDDING<br />
  15. 15. TISSUE EMBEDDING<br /> The process by which Tissues are orientated in the desired direction and are surrounded by a medium such as wax which when solidified will provide sufficient external support during sectioning<br />
  16. 16. HISTOLOGY PROCEDURE<br />SPECIMEN ACCESSIONING<br />TISSUE FIXATION<br />GROSS EXAMINATION<br />TISSUE PROCESSING<br />DEHYDRATION<br />CLEARING<br />IMPREGNATION <br />TISSUE EMBEDDING<br />TISSUE SECTIONING<br />
  17. 17. TISSUE SECTIONING<br /> The process by which wax hardened tissues are cut into very thin sections, 3-5 microns, using a Microtome<br />
  18. 18. HISTOLOGY PROCEDURE<br />SPECIMEN ACCESSIONING<br />TISSUE FIXATION<br />GROSS EXAMINATION<br />TISSUE PROCESSING<br />DEHYDRATION<br />CLEARING<br />IMPREGNATION <br />TISSUE EMBEDDING<br />SLIDE STAINING<br />TISSUE SECTIONING<br />
  19. 19. SLIDE STAINING<br />The process by which thin sections of tissue are stained using special dyes in order to enhance contrast in the microscopic image<br />
  20. 20. HISTOLOGY PROCEDURE<br />SPECIMEN ACCESSIONING<br />TISSUE FIXATION<br />GROSS EXAMINATION<br />TISSUE PROCESSING<br />DEHYDRATION<br />CLEARING<br />IMPREGNATION <br />TISSUE EMBEDDING<br />SLIDE STAINING<br />TISSUE SECTIONING<br />
  21. 21. THANK YOU!<br />contact@medimeas.com<br />

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