This document provides instructions for preparing tissue specimens embedded in paraffin blocks for sectioning. It involves placing tissue cassettes containing paraffin-infiltrated tissue in a heated water bath, warming empty metal molds, and using heated tools to carefully orient and embed the tissue specimens in hot paraffin within the molds before allowing the blocks to harden on a chilled plate.

2. 1. Place the Paraffin infiltrated tissue cassettes
in Tissue Embedding Station (TES) ’s Paraffin
Bath at 65° C for 15 minutes.
2. Warm the metal block molds in the warm
storage area of TES
3. Remove the paraffin infiltrated tissue from
the tissue cassette and using a blade dissect
out the infiltrated tissue.

3. 4. Place the empty mold on top of hot plate of TES
5. Pour a little Hot Paraffin from the reservoir of TES
into the mold and place the tissue specimen on
top of it
6. Using heated forceps orientate the tissue
specimen as desired and pour hot paraffin
7. Place the labeled empty tissue cassette on top of
mold and pour some more hot paraffin so as to
cover plastic cassette

4. 8. Shift the mold from the hot plate to the cold
plate (-15°C) of Tissue Cooling Station
9. When the wax is hard, take out the paraffin
block from the mold
10. The paraffin block is now ready for
sectioning