Tissue processing involves several key steps to prepare tissue samples for examination under a microscope. The steps include fixation to preserve tissue structure, dehydration to remove water, clearing with a solvent like xylene to remove alcohol, infiltration with paraffin wax, embedding wax-filled tissue in blocks, sectioning blocks with a microtome, staining sections with dyes like hematoxylin and eosin, and mounting on slides for viewing. The overall goal of tissue processing is to maintain tissue structure while making it suitable for thin sectioning and examination under a microscope.

1. Tissue processing
PRESENTED BY ANIKET CHOUDHARY

2. DEFINATION OF TISSUE PROCESSING
Tissue processing is the
technique by which fixed
tissue are made suitable for
embedding within a
supportive medium such as
paraffin.

3. TISSUE PROCESSING CONSIST OF SOME STEPS :-
1. FIXATION
2. DEHYDRATION
3. CLEARING
4. INFILTRATION
5. EMBEDDING
6. BLOCK MAKING
7. SECTION CUTTING
8. ROUTINE STAINING
9. MOUNTING

4. 1. FIXATION
 Fixation is a chemical process by which biological
tissue are preserved and hardening of the organ
in such a way that its original form is retain and
its constituents do not spread out.
 Commonly used fixative in lab
 10% formalin
 Formal saline
 10% buffered formaline
 Alcoholic formaldehyde

5. 2. DEHYDRATION
Dehydration is a process of removal of
water from tissue.
Alcoholic remove unbound water from
tissue sent to a alcohol 70%, 80%, 95%,
ethanol , methyl and action can be used.

6. 3. CLEARING
Zylene is commonly used for clearing a
tissue.
Zylene is helpful in removal of alcohol it
fixed tissue.
Zylene, toluene, benzene, chloroform can
be as a clearing agent.

7. 4. INFILTRATION
 Infiltration is the process why which clearing
agent is completely remove from the tissue.
 Clearing agent is estimated from the tissue by
defusion in the surrounding.
 The process when be added the melted
paraffin wax in tissue is called the infiltration.

8. 5. EMBEDDING
 The principal of embedding is to accurately
and presively oriented a histological sample of
the tissue into a block of paraffin wax.
 To L-mould are used for making a block will
allow the support and for to hold the tissue so
that find cutting and thick section will be cut
easily in a microtomes.

9. 6. BLOCK MAKING
 Metals L-moulds smeared with paraffin wax
 Orient sample so that intend the cutting surface is
preserved against base.
 Fill the moulds with fresh metal wax.
 Place identifying label.
 When block completely solidity remove mould.
 Block ready for cutting.

10. 7. Section cutting
Section cutting by using the microtome:-
Fix the block holder of the microtome.
Insert tightly a knife in the knife holder
with proper position.
Cut the sufficient section.

11. 8. ROUTINE STAINING
“H” and “E” staining can be
used as a routine stain.
“H” and “E” means
Hematoxyline and Eosin.

12. 9. MOUNTING
Mounting by using DPX.
Help to keep a sample in plays during
imagine.
Prevent the specimen from drying out.
Preserve sample over time for long term
storage.

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