This document provides information on the preparation and separation of plasma and serum from whole blood. It explains that plasma can be separated from whole blood by transferring blood to a tube containing anticoagulant and centrifuging, while serum is separated from clotted whole blood. The document discusses proper storage methods for plasma and serum, such as refrigeration or freezing. It also outlines some common errors in plasma/serum preparation like hemolysis and provides tips to prevent hemolysis.
Erythrocyte Sedimentation Rate (ESR), a lecture for medical lab technicians at Baquba Technical Institute, Middle Technical University. All theoretical and practical notes about the test.
ELISA- Principle, procedure , types and applicationsJaskiranKaur72
Enzyme-linked immunosorbent assay (ELISA) is a labeled immunoassay that is considered the gold standard of immunoassays.
This immunological test is very sensitive and is used to detect and quantify substances, including antibodies, antigens, proteins, glycoproteins, and hormones.
The detection of these products is accomplished by complexing antibodies and antigens to produce a measurable result.
Biochemistry is a basic science which deals with chemical nature and chemical behaviour of living matter and with the reactions and processes they undergo.
Biochemistry involves the study of:
Chemical constituents of living matter.
Chemical changes which occur in the organism during digestion, absorption and excretion.
Chemical changes which occur during growth and multiplication of the organism.
Transformation of one form of chemical constituent to the other.
Energy changes involved in such transformation.
Note:- The term “Biochemistry” was first introduced by German chemist Carl Neuberg in 1903 from Greek word “bios” means “life”.
It is mainly deals with the biochemical aspects that are involved in several conditions.
The results of qualitative and quantitative analysis of body fluids assist the clinicians in the diagnosis, treatment and prevention of the disease and drug monitoring, tissue and organ transplantation, forensic investigations and so on.
Various biological fluids subjected to chemical tests and assays include blood, plasma, serum, urine, cerebrospinal fluid (CSF), ascetic fluid, pleural fluid, faeces, calculi and tissues.
Note:- Modern day medical practice is highly dependent on the laboratory analysis of body fluids, especially the blood. The disease manifestations are reflected in the composition of blood and other tissues.
Hence, the demarcation of abnormal from normal constituents of the body is another aim of the study of clinical biochemistry.
A blood group also called a Blood Type
Classification of blood is based on the presence or absence of inherited antigenic substances on the surface of red blood cells (RBCs)
These antigens may be proteins, carbohydrates, glycoproteins, or glycolipids, depending on the blood group system.
The ABO blood group system is the most important blood type system (or blood group system) in human blood transfusion.
ABO blood types are also present in some other animals for example rodents and apes such as chimpanzees, bonobos and gorillas.
Notes about blood hemoglobin estimation, lecture notes to Medical Laboratory Students at Medical Laboratory Technology, Middle Technical University, Baqubah, Iraq
Erythrocyte Sedimentation Rate (ESR), a lecture for medical lab technicians at Baquba Technical Institute, Middle Technical University. All theoretical and practical notes about the test.
ELISA- Principle, procedure , types and applicationsJaskiranKaur72
Enzyme-linked immunosorbent assay (ELISA) is a labeled immunoassay that is considered the gold standard of immunoassays.
This immunological test is very sensitive and is used to detect and quantify substances, including antibodies, antigens, proteins, glycoproteins, and hormones.
The detection of these products is accomplished by complexing antibodies and antigens to produce a measurable result.
Biochemistry is a basic science which deals with chemical nature and chemical behaviour of living matter and with the reactions and processes they undergo.
Biochemistry involves the study of:
Chemical constituents of living matter.
Chemical changes which occur in the organism during digestion, absorption and excretion.
Chemical changes which occur during growth and multiplication of the organism.
Transformation of one form of chemical constituent to the other.
Energy changes involved in such transformation.
Note:- The term “Biochemistry” was first introduced by German chemist Carl Neuberg in 1903 from Greek word “bios” means “life”.
It is mainly deals with the biochemical aspects that are involved in several conditions.
The results of qualitative and quantitative analysis of body fluids assist the clinicians in the diagnosis, treatment and prevention of the disease and drug monitoring, tissue and organ transplantation, forensic investigations and so on.
Various biological fluids subjected to chemical tests and assays include blood, plasma, serum, urine, cerebrospinal fluid (CSF), ascetic fluid, pleural fluid, faeces, calculi and tissues.
Note:- Modern day medical practice is highly dependent on the laboratory analysis of body fluids, especially the blood. The disease manifestations are reflected in the composition of blood and other tissues.
Hence, the demarcation of abnormal from normal constituents of the body is another aim of the study of clinical biochemistry.
A blood group also called a Blood Type
Classification of blood is based on the presence or absence of inherited antigenic substances on the surface of red blood cells (RBCs)
These antigens may be proteins, carbohydrates, glycoproteins, or glycolipids, depending on the blood group system.
The ABO blood group system is the most important blood type system (or blood group system) in human blood transfusion.
ABO blood types are also present in some other animals for example rodents and apes such as chimpanzees, bonobos and gorillas.
Notes about blood hemoglobin estimation, lecture notes to Medical Laboratory Students at Medical Laboratory Technology, Middle Technical University, Baqubah, Iraq
Since antigen and antibody reactions are specific, they can be used to identify each other.
These diagnostic tests are particularly useful in diagnosing for examples: infectious diseases, autoimmune diseases, and in typing of blood and tissues prior to transplantation.
Hematology is the branch of medicine, that is concerned with the study of blood, blood forming organs and blood diseases. It includes study of etiology, diagnosis, treatment, prognosis and prevention of blood diseases .
After the completion of this presentation we will know about:
What is hematology and its purpose.
hematology laboratory.
Blood and its compositions and collections
Hematology lab equipment's
Some hematological tests , disease and hazards too.
Our bodies are constantly under attack by an army of microorganisms, toxins, allergens and other substances that are recognized as foreign (non-self).
The ways in which the body protects itself from pathogens can be thought of as an army consisting of three lines of defense.
Immune system consists of two organs primary and secondary lymphoid organs.
1-Primary (Central) lymphoid organs.
2-Secondary (Peripheral) lymphoid organs.
1-Thymus Gland.
2-Bone Marrow.
They consist of ….
Spleen.
Lymph nodes.
Mucosa- associated lymphoid tissue.
The sites of immune cell birth and the locations where they mature are considered primary lymphatic organs.
Locations in the body where immune cells become activated, reside, or carry out their functions are called secondary lymphatic organs
The cells of the immune system arise from a pluripotent Hematopoietic Stem Cells (HSCs) through a process known as haematopoiesis.
Hematopoiesis involves the production, development, differentiation, and maturation of the blood cells (erythrocytes, megakaryocytes and leukocytes) from HSCs.
Differentiation of the HSC will occur along one of two pathways, giving rise to either a common myeloid progenitor or a common lymphoid progenitor cells in the presence of specific cytokines or soluble mediates (growth factor).
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
MANAGEMENT OF ATRIOVENTRICULAR CONDUCTION BLOCK.pdfJim Jacob Roy
Cardiac conduction defects can occur due to various causes.
Atrioventricular conduction blocks ( AV blocks ) are classified into 3 types.
This document describes the acute management of AV block.
Basavarajeeyam is an important text for ayurvedic physician belonging to andhra pradehs. It is a popular compendium in various parts of our country as well as in andhra pradesh. The content of the text was presented in sanskrit and telugu language (Bilingual). One of the most famous book in ayurvedic pharmaceutics and therapeutics. This book contains 25 chapters called as prakaranas. Many rasaoushadis were explained, pioneer of dhatu druti, nadi pareeksha, mutra pareeksha etc. Belongs to the period of 15-16 century. New diseases like upadamsha, phiranga rogas are explained.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
2. Preparation and Separation of
Plasma:
The blood is transferred from a person’s vein to a test
tube and prevented from clotting (tube containing
anticoagulant e.g. Heparin, EDTA, Sodium Citrate,
Oxalate, Sodium Fluoride, Sodium Iodoacetate ) , this
preparation should be mixed immediately and
thoroughly to avoid clotting, and centrifuged at 2500-
3000 rpm for 5-10 min.
3. Preparation and Separation of
Plasma:
It separates into two layers. The upper liquid layer,
called plasma, represents about 55% of the volume
of whole blood. The lower layer consists of red blood
cells (erythrocytes), white blood cells (leukocytes),
and blood platelets (thrombocytes). Collectively,
these are called the formed (cellular) elements and
represent about 45% of the volume of whole blood.
4. Preparation and Separation of
Serum:
• If blood is transferred from a person’s vein to a test tube
without containing anticoagulant and then allowed the
blood to clot at room temperature for 15 to 30 minutes,
when the blood has clotted completely and then
centrifuged at 2500-3000 rpm for 5-10 min. it separates into
two layers, the upper liquid layer called serum and the
lower layer consists of formed elements.
• Serum =Plasma- Fibrinogen(coagulant factor)
5.
6. Laboratory Specimens:
Most Common
•Blood samples
•Urine samples
Swabs
•Vaginal swabs
•Wound swabs
•Nose swabs
•Pernasal swabs
•Ear swabs
•Eye swabs
•Throat swabs
Additional
•Biopsy material
•Cerebrospinal fluid
•Stool samples
•Fungal samples of hair, nail and skin
•Naso-pharyngeal aspirate
•Sputum
•Semen
•Vaginal secretion
•Saliva
•Tears
•Cerumen (earwax)
•Sweat
Body Fluid
•Percardial fluid
•Pleural fluid
•Synovial fluid
•Vitreous humour
•Amniotc fluid
7. Important note:
• The color of the serum and plasma must be clear yellow
and there is no any turbidity (cloudy) or if any white color
it shows the high proportion of fat in it which affects the
result of the analysis.
• Similarly, if the color is reddish, it indicates the breaking of
red blood cells, which affects a significant impact on some
of the results.
8. Which is more appropriate Plasma or Serum
or whole blood for doing blood biochemical
estimations?
• It depends on type of analyte to be determined.
• You better use serum rather than plasma.
Anticoagulate in the plasma interferes with many
biochemical parameters particularly trace
elements as lead and mercury are bond
preferably on erythrocytes, therefore for its
determination, the whole blood is preferred.
9. Method for Serum Preservation
(Storage):
• In normal condition and the right is that the samples are direct do test
without delay but, if necessary keeping the sample must be following
in mind:
• Must separated sample and stored at low temperature in the
refrigerator or freezer.
• Never preserves whole blood samples such as complete blood count
in the freezer.
• Some samples have special conditions, such as the conservation
Bilirubin must be kept in the dark.
• There are samples should never delay ,such as analysis of
prothrombin and erythrocyte sedimentation analysis .
10. Method for Serum Preservation
(Storage):
1. Preservation for short time,freezing method is followed between
(-4 to -28°C).
2. Preservation for Long time, freezing method is (-86°C).
Benchtop FreezerUpright Freezer
11. Notes:
• How long can frozen human serum be stored?
Frozen blood can be stored for 10 years from the date of
blood collection.
• It is important to avoid freeze-thaw cycles because this is
detrimental to many serum components.
• The serum put in polypropylene microcentrifuge tube.
12. Common Serum or Plasma Preparation
Errors:
1-Failure to separate serum or plasma from red cells within 30 to 45
minutes of venipuncture.
2-Hemolysis (occurs when the membrane surrounding red blood cells
is disrupted and hemoglobin and other intracellular components
escape into the serum or plasma. Hemolyzed serum or plasma varies in
color from faint pink to bright red, rather than the normal straw color.
Grossly or moderately hemolyzed specimens may be rejected and even
slight hemolysis may alter certain test results).
13. How To Prevent Hemolysis:
1. Do not leave tourniquet for longer than one minute.
2. Allow alcohol to dry completely before puncturing the
skin.
3. Use a properly sized needle for routine collections.
4. Do not remove the needle from the vein with the vacuum
tube still engaged.
5. Make sure sample is not exposed to extreme heat or cold.
6. Allow blood to clot completely prior to centrifugation.
7. Avoid vigorous mixing or shaking of tubes.
8. Do not centrifuge specimens at higher speed or for longer
than necessary.
14. Differences between in vitro, in vivo, and in
silico studies
There are three broad categories of experiments:
In vitro (Latin for within the glass) refers to the technique of performing
a given procedure in a controlled environment outside of a living
organism.
In vivo (Latin for “within the living”) refers to experimentation using a
whole, living organism as opposed to a partial or dead organism.
In silico is an expression used to mean “performed on computer or via
computer simulation.
15. Serological
Reactions:
• Antigen-antibody reaction (binding) in vitro are known as
serological reactions, its useful for detection and
measurement either antibody (Ab) or antigen (Ag) in the
serum.
Uses:
1- Diagnosis of infections.
2- Evaluation of the immunological status.
16. • Antigens (Ag) : Are molecules (substances) recognised by
the immune system, which induce an immune response
examples bacteria, viruses, parasites & fungi.
• Antibodies (Immunoglobulin): Are proteins produced by
plasma cells in response to stimulation of B cell by foreign
antigen, basic structure of immunoglobulin is Y shaped
structure, there are five immunoglobulin classes of antibody
molecules found in serum: IgG, IgM, IgA, IgE and IgD.
Some Concept:
17. Serological Techniques and Immune
Assays:
•Numerous types of serologic test differ in their speed and
sensitivity, so the most effective test have high specificity and
sensitivity, some are strictly qualitative (positive or negative)
and others are quantitative (amount measured).
18. Serological Techniques and Immune
Assays:
There are several different methods used in serological tests:
1.Agglutination test.
2.Precipitation test.
3.Labeled Immune assay (immunoassay).
A.Radioimmunoassay (RIA).
B.Fluorescent immunoassay (FIA).
C.ElectroChemiluminescent immunoassays (ECL).
D.Enzyme immunoassay (EIA) & Enzyme linked immunosorbent assay
(ELISA).
19. Principle of Serological Tests:
• A reaction between antigen (Ag) and antibody
(Ab) to produce a DETECTABLE reaction.
• Antigen-antibody reaction are visible by clumps,
precipitates, color changes, emitting photons &
release of radioactivity.
20. Agglutination Reaction
(test):
• The interaction (immune reaction) between antibody and a
particulate antigen resulting a visible clumping called
agglutination. Antibodies that produce such reactions are
called agglutinins while antigens are called agglutinogen.
• Agglutination: This reaction done between Ab & insoluble
Ag is part of the surface of some particulate material (such
as Erythrocytes, Bacterial cells, Inert carriers such as
polystyrene latex particles).
• Purpose: Agglutination reactions used to detect either the
presence of antigen or antibody in a sample.
21. Figure: Phases of agglutination. (Stevens, 2010) A) Sensitization, physical
attachment of Ag & Ab. B) Network formation.
22. Clinical Applications of agglutination test:
(Reagents)
• ANA (Antinuclear antibody) test for diagnosis nuclear autoantibodies.
• ASO (Antistreptolysin O) test for diagnosis of Streptococcus bacteria.
• Brucella agglutination test for diagnosis of brucellosis.
• CRP (C-Reactive Protein) to check for inflammation in the body.
• IM (Infectious Mononucleosis) or Monospot screening test for infections
mononucleosis.
• Latex test for rheumatoid factor.
• PT (Pregnancy testing) for detection Beta-HCG.
• RF (Rheumatoid factor test) detects autoantibodies present in Rheumatoid
arthritis.
• TPHP (Treponema pallidum particle agglutination assay) screening test for syphilis.
• VDRL (Venereal Disease Research Laboratory test) screening test for syphilis.
• Widal test for diagnosis of salmonellosis.
Fahrenheit to Celsius (ºF to ºC)
http://www.metric-conversions.org/temperature/fahrenheit-to-celsius.htm
WHY Bilirubins are usually in tubes and kept in dark tubes and out of the light? Because bilirubin break done by light
Fahrenheit to Celsius (ºF to ºC)
http://www.metric-conversions.org/temperature/fahrenheit-to-celsius.htm
fridge
WHAT ARE DOING IF YOUR SAMPLE IS HEMLOYSIS?
The use of laboratory animals (mice, guinea pigs, rabbits) is now limited due to the advancement in medical microbiological techniques.
In silico techniques are developed particularly as an alternative to animal experimentation.