Ensuring potable water for public consumption is a major Public Health Concern. This presentation sums up all the necessary and prioritized parameters conducted for water analysis.
This Presentation Clarifying about potable Water analysis and their methods which i gave training on operation and maintenance team for Oman Al Ghubrah Independence Water Project (SWRO Desalination 42 MIGD)
Here you will find brief description about water sampling. actually it's so important to examine the water we use our daily life in order to avoid negative impact of water.
The presentation gives a idea about the methods for water analysis. The parameters included are testing pH, hardness, sulphate, phosphorus, COD and many other parameters.
This Presentation Clarifying about potable Water analysis and their methods which i gave training on operation and maintenance team for Oman Al Ghubrah Independence Water Project (SWRO Desalination 42 MIGD)
Here you will find brief description about water sampling. actually it's so important to examine the water we use our daily life in order to avoid negative impact of water.
The presentation gives a idea about the methods for water analysis. The parameters included are testing pH, hardness, sulphate, phosphorus, COD and many other parameters.
This presentation describes, how the water can be analyzed using the basic parameters like DO, COD, BOD, Alkalinity and so on. The physical, chemical, and biological parameters were discussed here.
Water has its own taste, color, smell and constituents. Not all water can be used for all purposes. Eg. Sea water can not be used by us for drinking. The suitability of water for different purposes is determined by its quality parameters. The Quality of water is equally important than quantity. Even if present in huge amounts, we can not use salt water in many life support activities. Water has its own Physical properties, Chemical composition and Biological Properties. This module highlights the water quality parameters that are essential.
This presentation discusses the drinking water quality parameters, drinking water quality standards, water quality index and classification of water bodies and standards
Quality of water :
It includes all the physical, chemical and biological parameters along with test to be used for defining water quality and water schemes for city
wholesomeness, Requirements for Domestic Use. Impurities in Water. Objects & purpose of Water Analysis.Collection of Samples. Classification of Analysis of Water: Physical,
Chemical & Biological Examination of Water.
Nearly all water in the world contains contaminants, even in the absence of nearby pollution-causing activities
Many dissolved minerals, carbon compounds, and microbes find their way into drinking water as it comes in contact with air and soil
When pollutant and contaminant levels in drinking water are high, they may affect household routines and be detrimental to human health
The only way to ensure that your water supply is safe is to have a periodic laboratory water quality analysis done on your drinking water. Hach India is the leading provider of high end water quality analysis equipment in india
This presentation describes, how the water can be analyzed using the basic parameters like DO, COD, BOD, Alkalinity and so on. The physical, chemical, and biological parameters were discussed here.
Water has its own taste, color, smell and constituents. Not all water can be used for all purposes. Eg. Sea water can not be used by us for drinking. The suitability of water for different purposes is determined by its quality parameters. The Quality of water is equally important than quantity. Even if present in huge amounts, we can not use salt water in many life support activities. Water has its own Physical properties, Chemical composition and Biological Properties. This module highlights the water quality parameters that are essential.
This presentation discusses the drinking water quality parameters, drinking water quality standards, water quality index and classification of water bodies and standards
Quality of water :
It includes all the physical, chemical and biological parameters along with test to be used for defining water quality and water schemes for city
wholesomeness, Requirements for Domestic Use. Impurities in Water. Objects & purpose of Water Analysis.Collection of Samples. Classification of Analysis of Water: Physical,
Chemical & Biological Examination of Water.
Nearly all water in the world contains contaminants, even in the absence of nearby pollution-causing activities
Many dissolved minerals, carbon compounds, and microbes find their way into drinking water as it comes in contact with air and soil
When pollutant and contaminant levels in drinking water are high, they may affect household routines and be detrimental to human health
The only way to ensure that your water supply is safe is to have a periodic laboratory water quality analysis done on your drinking water. Hach India is the leading provider of high end water quality analysis equipment in india
Effluent Treatment Plant Design, Operation And Analysis Of Waste Water Jaidev Singh
Contents
1. Introduction to Effluent Treatment Plant (ETP)
1.1 Use of water in industries
1.2 Industrial waste water sources
1.3 Effluent Treatment Plant
1.4 National Standards for waste water
1.5 What do these standards means?
1.6 Waste water treatment
1.7 Planning an Effluent Treatment Plant : Factors to Consider
2. Treatment Methods
2.1 Physical Unit Operations
2.2 Chemical Unit Processes
2.3 Biological Unit Processes
3. Operation and control
3.1 Mixed liquor suspended solids
3.2 Sludge Volume Index and Sludge Density Index
3.3 Sludge Age; Mean Cell Residence Time (MCRT)
3.4 Food/Mass Ratio
3.5 Constant MLSS
3.6 Return Activated Sludge Control (RAS)
4. Choosing an Effluent Treatment Plant
4.1 Biological Treatment
4.2 Physico-Chemical Treatment
4.3 Physico-Chemical and Biological Treatment
4.4 Area Requirement Comparison
4.5 Cost Comparison
5. Chemical Analysis of Waste Water
5.1 Commonly used chemicals
5.2 Chemical Tests and procedures
NAVMED P-5010, Manual of the Naval Preventive Medicine, Chapter 6Shayne Morris
This Power Point is part of an Enlisted Advancement Program training series for US Navy Corpsman rating provided by Naval Medical Center Portsmouth Virginia
Naval Medical Center Portsmouth is a military treatment facility serving active duty service members, their dependents and retirees in the Hampton Roads community of southeastern Virginia and northeastern North Carolina.
INDOLE TEST
UREASE TEST
CITRATE TEST
METHYL RED(MR) TEST
VOGES – PROSKAUER(VP) TEST
TRIPLE SUGAR IRON(TSI) TEST
OXIDASE TEST
CATALASE TEST
CATALASE TEST-Principle:-
This test demonstrates presence of catalase enzyme. This enzyme catalyses the release of O2 from H2O2.
catalase
2H2O2 H2O + increase O2
Reagents:- 1) 3% H2O2.
2) 24 hrs cultured organisms
Procedure:-
With sterile wooden stick transfer culture organisms to test tube containing 3% H2O2 and observe for production of effervescence.
It can also be tested directly on growth plate.
Positive Control: Staphylococci.
Negative Control: Streptococci.
False positive reactions:
If culture medium contains catalase enzyme e.g., blood agar, chocolate agar.
If iron wire loop is used
recent microbial techniques & advancement in identifying, cultivating,& handl...Karunanidhan3
I tried to include all techniques & diseases that are included in Pharm D 2nd year microbiology syllabus as per PCI. Do suggest if i have to improve my writing skills, on officialkarunanidhan@gmail.com
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Ethnobotany and Ethnopharmacology:
Ethnobotany in herbal drug evaluation,
Impact of Ethnobotany in traditional medicine,
New development in herbals,
Bio-prospecting tools for drug discovery,
Role of Ethnopharmacology in drug evaluation,
Reverse Pharmacology.
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Students, digital devices and success - Andreas Schleicher - 27 May 2024..pptxEduSkills OECD
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The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
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The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
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How libraries can support authors with open access requirements for UKRI fund...
Water Analysis for Drinking Water
1.
2. A. Physical Aspects
1. Taste
2. Odor
3. Color
4. Turbidity
5. pH
6. Total Dissolved Solids
B. Chemical Aspects
1. Arsenic
2. Cadmium
3. Lead
4. Benzene
5. Iron
6. Manganese
7. Chloride
8. Sulfate
** Chlorine Residual
C. Radiological
Aspects
1. Gross Alpha Activity
2. Gross Beta Activity
3. Radon
D. Microbiological
Aspects
1. Multiple Tube Fermentation
Technique
2. Membrane Filter Technique
3. Chromogenic Substrate Test
4. Heterotrophic Plate Count
3. This part deals primarily with the
measurement of the physical properties of
the sample
Provides first warning of potential hazards in
the environment
4. Refers to the gustatory sensations
◦ Bitter – caffeine ( Strong coffee)
◦ Salty – sodium chloride (Chlorinated water)
◦ Sour – citric acid (lemon juice)
◦ Sweet – sugar (syrup)
Flavor – gustatory, olfactory and trigeminal
sensations
Methods:
◦ Sensory Evaluation Technique
Only used on samples known to be safe for
ingestion
Standard: No Objectionable Taste
5. Affected by organic and inorganic chemicals
present in water
Provide qualitative descriptions of odor
intensity
Method:
◦ Sensory Evaluation Technique
Standard: No Objectionable Odor
6.
7. Color of surface and groundwater results
primarily from the presence of natural
organic matter, particularly aquatic humic
matter
◦ Humic matter consists of humic and fulvic acids –
yellow-brown color
Substances that adds color to water:
◦ Suspended particles
Clays, algae, iron, manganese oxides
Color usually removed from potable water for
aesthetic purposes
8. True Color
◦ Color of water from which the turbidity has been
removed
◦ Standard: ≦ 5 Color Units
Apparent Color
◦ Color due to substances in the solution and
suspended matter
◦ Contributed principally by colloidal or suspended
material
◦ Standard: ≦ 10 Color Units
Methods: Visual Comparison Colorimetric
9. Caused by suspended and colloidal material
◦ Clay, silt, finely divided organic and inorganic
matter, plankton and other microbes
Method: Turbidimetry
Standard: 5 NTU
10. Acidity
◦ Attributed to the presence of strong mineral acids and
weak acids and hydrolizing salts such as iron and
aluminum sulfate
◦ Contribute to corrosiveness and influence chemical
rates, chemical speciation and biological processes
Alkalinity
◦ Refers to the acid-neutralizing capacity of water
◦ Attributed to the carbonate, bicarbonate and hydroxide
content and borates, phosphates and silicates or other
bases
Method: Electrometric method (pH meter)
Standard: 6.5 to 8.5
11. Waters with high dissolved solids generally
are of inferior palatability
Portions of solids that passes through a filter
of nominal pore size
Methods: Gravimetric, dried at 180°C
Standard: ≦ 500
12. Various forms of chemicals are present in
drinking water that may lead to some health
problems
Inorganic constituents
◦ Arsenic, lead, chromium and cadmium
Suspected carcinogens
Organic constituents
◦ Due to decomposition of organic debris, domestic,
agricultural, industrial, water treatment
13. Presence of arsenic in water is due to mineral
dissolution, industrial discharges, application of
pesticides
Health Effects: severe poisoning and carcinogenic
Methods: Inductively Couple Plasma/Mass
Spectrophotometry; hydride generation Atomic
Absorption Spectrophotometry; Silver
Diethyldithiocarbamate Method, Electrothermal
Atomic Absorption Spectrophotometry (Graphite
furnace AAS)
Standard: ≦ 0.05 mg/L
14. extremely toxic and accumulate in the
kidneys and liver
◦ Causes dysfunction of the kidneys
Methods: Inductively Couple Plasma/Mass
Spectrophotometry (ICP/MS), Flame Atomic
Absorption Spectrophotometry (FAAS)
Standard: ≦ 0.003 mg/L
15. Presence in water supply comes from industrial,
mine and smelts discharges or from the
dissolution of plumbing and plumbing fixtures
Toxic and cummulative poison
Method: Inductively Couple Plasma/Mass
Spectrophotometry (ICP/MS), Flame Atomic
Absorption Spectrophotometry (FAAS),
Electrothermal Atomic Absorption
Spectrophotometry (EAAS), Anodic Stripping
Voltammetry; Dithizone
Standard: ≦ 0.01 mg/L
16. Results with high contamination from human
or animal waste
Methods: Cd Reduction Method, Capillary Ion
Electrophoresis Colorimetric (Diazotization);
Flow Injection Analysis
Standard: ≦ 0.01 mg/L
17. Presence is due to industrial effluents,
atmospheric pollution due to vehicular
emissions
Method: Gas Chromatography/Mass
Spectrophotometry
Standard: ≦ 0.01 mg/L
18. Elevated iron levels can cause stains in
plumbing, laundry, and cooking utensils
Imparts objectionable taste and color
Ferrous state -> oxidized -> ferric state
(reddish precipitate)
Method: Phenanthroline, Atomic Absorption
Spectrophotometry, Colorimetric Method
Standard: ≦ 0.01 mg/L
19. Elevated manganese can cause stains in
plumbing, laundry, cooking utensils
Mn2+ -> oxidized -> MnO2 black precipitate
Methods: Perfurate Method, Atomic
Absorption Spectrophotometry, Inductively
Color Plasma/Mass Spectrophotometry
Standard: ≦ 0.04 mg/L
20. Major inorganic anions in water
Produces salty taste if the cation is sodium
High chloride content may harm metallic
pipes and structures
Method: Argentometric Method, IC
Standard: ≦ 250 mg/L
21. Occurs naturally in water
Method: Turbidimetric Method, Ion
Chromatography, Gravimetric Method
Standard: ≦ 250 mg/L
22. Chlorine if not within limits may produce
adverse effects
Taste and color of water is intensified
Potential organic chloroform may be form in
the process of chlorination
Methods: Iodometric, Amperometric
Titrations, DPD Colorimetric Method
Standard: 0.3 to 1.5 mg/L
23. Does not provide a complete water quality
picture
◦ If possible a series of analysis must be conducted
over a protracted period
24. Coliform group consists of several genera of
bacteria belonging to the family of
Enterobacteriaceae
◦ Facultative anaerobic, gram-negative, non-sporeforming
bacteria that ferments lactose with
gas and acid within 48 hours @ 35°C
Uses MPN table to estimate coliform density
25. Presumptive Phase
◦ Lauryl Sulfate Broth (Triple Strength)
10mL media plus 20mL sample in 5 tubes
Incubate for 48hours at 35 +/- 0.5°C
Confirmatory Phase
◦ Brilliant Green Bile Broth
1 loopful or 1 drop of positive samples in 5 tubes of 10mL
media
Incubate for 48hours at 35 +/- 0.5°C
◦ EC Broth
1 loopful or 1 drop of positive samples in 5 tubes of 10mL
media
Incubate in water bath for 24 hours at 44.5 +/- 0.2°C
Completed Phase
◦ Lauryl Sulfate Broth (Single Strength)
26. Presumptive Phase
Preparation of Lauryl Sulfate Broth
Dissolve 35.6 grams in one liter distilled water.
Dispense 10 ml to clean culture tubes.
Place fermentation/durham tube in an inverted position.
Put the screw cap on the culture tubes.
Place all tubes in the autoclavable rack and wrap with brown paper.
Sterilize for 15 minutes at 121 psi pressure.
Procedure
Pipette 20 ml of water sample into five (5) tubes of primary lactose broth.
Mix by gentle shaking.
Incubate at 35°C for 24 hours.
Read at the end of 24 hours and record the presence or absence of gas.
Incubate for another 24 hours if no gas has developed.
Examine for gas formation after 48 hours.
27. Confirmatory Test
Brilliant Green Bile Broth
Preparation
Dissolve 40 grams in one liter distilled water.
Dispense 10 ml to clean culture tubes.
Place fermentation/durham tube in an inverted position.
Put the screw cap on the culture tubes.
Place all tubes in the autoclavable rack and wrap with brown paper.
Sterilize for 15 minutes at 121 psi pressure.
Procedure
Pipette 0.3 ml of broth from positive presumptive tubes to five (5) to ten
(10) ml portions of BGB tubes.
Mix by gentle shaking.
Incubate the inoculated tubes for 24-48 hours, at 35°C.
28. Confirmatory Test
EC Media
Preparation
Dissolve 37 grams in one liter distilled water.
Dispense 10 ml broth to culture tubes.
Place fermentation tube in an inverted position.
Put the screw cap on the culture tubes.
Place all tubes in the autoclavable rack and wrap with brown
paper.
Sterilize for 15 minutes at 121 psi pressure.
Procedure
Transfer 0.3 ml water sample from positive presumptive tubes.
Incubate at 44.5 ± 2 °C for 24 hours in water bath.
29. Used to test large sample volumes
Released numerical results more rapidly than
MTFT
Greatly affected by:
◦ high levels of turbidity
Clogs the membrane filter
◦ High heterotrophic bacteria count
Interferes with the growth of coliforms on the filter
31. Also called Enzyme Substrate Coliform Test
Qualitative test
Principle:
◦ Total Coliform Bacteria
Chromogenic Substrate
1. ONPG – ortho-nitrophenyl-β-D-galactopyranoside
2. CPRG – chlorophenol red- β-D-galactopyranoside
3. X-GAL – 5-chromo-4-chloro-3-indoyl- β-D-galactopyranoside
Detects enzyme β-D-galactosidase which is produced
by total coliform bacteria
32. Principle:
◦ Escherichia coli
Fluorogenic Substrate
1. MUG – 4-methylumbellferyl-β-D-glucoronide
o Detects enzyme β-D-glucoronidase which is
produced by E. coli
34. Useful in checking water treatments’
effectiveness
◦ Indirect indicator of pathogen removal
High HPC – false negative coliform results
a) Pour Plate – R2A, NWRI agar
b) Spread Plate – R2A, NWRI agar
c) Membrane Filter – mHPC, R2A, NWRI agar
39. “The analytical laboratory provides qualitative and
quantitative data for use in decision-making. To be
valuable, the data must accurately describe the
characteristics and concentrations of constituents in
the samples submitted to the laboratory. In many
cases, because they lead to faulty interpretations,
approximate or incorrect results are worse than no
result at all.”
40. set of operating principles that, if strictly followed
during sample collection and analysis, will produce
data of known and defensible quality
41. Everyone involved with the lab:
◦ Person sampling
◦ Person running the test
◦ Person washing the glassware
◦ Person doing maintenance on the instruments
◦ Person interpreting the results
42. Staff Organization and Responsibilities
Sample Control and Documentation
SOP for Analytical Methods & Procedures
Analyst Training Requirements
Equipment Preventative Maintenance
Calibration Procedures
Corrective Actions
Internal Quality Control Activities
Performance Audits
Data Assessment for Bias and Precision
Data Validation and Reporting
43. Documentation
Communication
Training
Cross-Training
Updating
44. part of quality management focused on fulfilling
quality requirements
examining “control” materials of known
substances along with patient samples to monitor
the accuracy and precision of the complete
examination (analytic) process
GOAL: to detect errors and correct them
before results are reported
45. Certification of Analyst Competence
Recovery of Known Additions
Analysis of Standards
Analysis of Reagent Blanks
Calibration with Standards
Analysis of Duplicates
Maintenance of Control Charts
Editor's Notes
The Accuracy of the analytical result can be stated with a high level of confidence.”