This document discusses Vibrio cholerae and cholera from microbiological and molecular perspectives. It begins with an introduction to cholera and its causative agent V. cholerae. It then covers the classification, virulence factors, and genomics of V. cholerae. Key points include that V. cholerae is a gram-negative, facultative anaerobe that produces cholera toxin and toxin-coregulated pilus which enable its pathogenicity. The document also summarizes the pandemics of cholera caused by classical and El Tor biotypes and discusses antibiotic resistance and the evolution of new V. cholerae variants.
Cholera is a acute diarrhoeal disease caused by Vibrio cholerae.
Majority of infection are mild or asymptomatic.
IV B.PHARM, 8-SEMESTER ,SOCIAL AND PREVENTIVE PHARMACY.
CHOLERA DISESASE
DEFINITION, SYMPTOMS, CAUSES, TREATMENT, PREVENTION.
Cholera is a acute diarrhoeal disease caused by Vibrio cholerae.
Majority of infection are mild or asymptomatic.
IV B.PHARM, 8-SEMESTER ,SOCIAL AND PREVENTIVE PHARMACY.
CHOLERA DISESASE
DEFINITION, SYMPTOMS, CAUSES, TREATMENT, PREVENTION.
Shigellosis = inflammation of intestines (especially the colon) with accompanying severe abdominal cramps, tenesmus and frequent, low-volume stools containing blood, mucus and fecal leukocytes.
Poxviruses are brick or oval-shaped viruses with large double-stranded DNA genomes. Poxviruses exist throughout the world and cause disease in humans and many other types of animals. Poxvirus infections typically result in the formation of lesions, skin nodules, or disseminated rash.
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
Shigellosis = inflammation of intestines (especially the colon) with accompanying severe abdominal cramps, tenesmus and frequent, low-volume stools containing blood, mucus and fecal leukocytes.
Poxviruses are brick or oval-shaped viruses with large double-stranded DNA genomes. Poxviruses exist throughout the world and cause disease in humans and many other types of animals. Poxvirus infections typically result in the formation of lesions, skin nodules, or disseminated rash.
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
consists of two cerebellar hemispheres joined by a narrow median vermis.
The cerebellum is connected to the posterior aspect of the brainstem by three symmetrical bundles of nerve fibers called the superior, middle, and inferior cerebellar peduncles
The Gram-negative A. actinomycetemcomitans is assumed to be the primary etiologic agent of LAgP and has also been implicated in chronic periodontitis and severe non-oral infections.
Moving into the Post-MetagenomicEra of Gut Microbiome ResearchJonathan Clarke
Julian Marchesi's presentation slides from our previous Microbiome R&D and Business Collaboration Forum. For information about this years event please visit http://www.globalengage.co.uk/microbiota.html
Gastrointestinal pathogens of the family Vibrionaceae: Include the following medically important genera: Vibio cholerae, Aeromonas, Campylobacter, and Helicobacter pylori.
ggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans) is a Gram-negative, facultative nonmotile, rod-shaped oral commensal often found in association with localized aggressive periodontitis, a severe infection of the periodontium, although it is also associated with nonoral infections. Its role in periodontitis was first discovered by Danish-born periodontist Jørgen Slots, a professor of dentistry and microbiology at the University of Southern California School of Dentistry.
'Bacterium actinomycetem comitans' was described by Klinger (1912) as coccobacillary bacteria isolated together with Actinomyces from actinomycotic lesions of man. It was reclassified as Actinobacillus actinomycetemcomitans by Topley & Wilson (1929) and as Haemophilus actinomycetemcomitans by Potts et al. (1985). The species has attracted attention because of its association with localized aggressive periodontitis. is explained here by Dr Harshavardhan Patwal
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
3. CONTENT
Introduction of Cholera.
Epidemics and Pandemics of Cholera.
Causative agent of Cholera.
Cholera and V. cholerae in the view of microbiological
perspective.
Molecular perspective of V. cholerae
Possible Future Threat and Research
References 3
4. CHOLERA; A BLUE DEATH
What is
Cholera?
Acute
Secretory
diarrheal
disease
Results rice
water stools
Agents
Gram
negative
bacteria
Vibrio
cholerae
Age
group
All age
group
Children <5
years are
more
susceptible.
4
5. MICROBIOLOGY OF V. cholerae
This section deals with-
The Causative agent of Cholera
Classification of Vibrio cholerae.
Virulence factors.
5
6. Vibrio Cholerae- THE CAUSETIVE AGENT
OF CHOLERA
• Gram negative
• Facultative anaerobic
• Non-Spore forming
Morphology
• 1.4 to 2.6µm long
• Coma shapedStructure
• ˜ 105 to108 bacteria is required for
successful infection
• Natural habitat is brackish or salt
water
Other
feature 6
8. SEROGROUPS OF V. CHOLERAE
8
Produce Cholera toxin
(CT) and Toxin co
regulated pillus (TCP)
Involve in epidemics
Mechanism of
pathogenesis is known
CT negative, but
contain toxin like Cholix
toxin.
Involve in sporadic and
localized out break
Pathogenic mechanism
is not clear.
O1 &O139 Non O1 & Non O139
9. EPIDEMIOLOGY OF CHOLERA
• Both epidemic and endemic
• Both seasonal and off seasonal
Pattern
• 1.3 to 4 million affected people.
• 143000 to 210000 deaths.
(WHO,2016)
Mortality
rate/year
• Poor sanitation.
• Transmission – either human to
human or environment to human
Reason
9
12. 12
Biotype Phenotypes Genotype
CCA PB VP Phage
IV
Phage
5 tcpA rstR ctxB
Classic
al
- s - s r Cla Cla B1
El Tor + r + r s El El B3
PANDEMICS; CLASSICAL VERSES El TOR
Abbreviations: CCA- Chicken Cell Agglutination
PB – Polymixin
VP- Voges-Proskaure
Key : +, positive ; -, negative; s , sensitive; r, resistance
Ref- Safa et al,.2010
15. OTHER VIRULENCE FACTORS INCLUDES
Factors Biological Function Responsible
gene
Accessory colonization
factors
Adhesion to mucosal cell acf
Hemagglutination
protease
Helps to dissolve
glycoprotein coating of
intestinal cell
Hap
Accessory cholera
enterotoxin
Release exotoxin ace
Other membrane protein Colonization assisting
mechanism
ompW
15
16. V. cholerae: MOLECULAR PERSPECTIVE
Genome organization
Cholera toxin
Molecular mechanism of action
Antibiotics resistance mechanism
Evolution of new variants
16
17. GENOME ORGANIZATION
2 Circular chromosome.
The largest one is 2.4 Mb and the smallest one is about 1.6
Mb in length.
17
Ref- Heidelberg J.F et al., 2000
18. GENETIC ORGANIZATION OF CTXΦ
18Figure- Bacteriophage CTXφ organization
Ref- Safa et al., 2010
19. CHOLERA TOXIN
A subunit
A1 segment-ADP
ribosylates of G
protein
A2 Segment-
Facilitate binding A
with B subunit by
forming a alpha
helix
B subunit
Attachment of Toxin
to the host cell.
19
Figure- Structure of CT
Ref: Naomi LB et al.,
20. MOLECULAR MECHANISM OF ACTION
Figure- Molecular mechanism of actions of Cholera toxin
20
21. ANTIBIOTIC RESISTANCE MECHANISM
• Widespread and
inappropriate use of
antibiotics
Causes
• Major threat to public health
because it results treatment
failure
Concern
• For cholera treatment –
Azithromycine.
Drug of
choice 21
22. FIGHT AGAINST ANTIBIOTICS
22
Type of
Antibiotic
resistance
Intrinsic
mechanism
Efflux pump
Spontaneous
mutation
Acquired
mechanism
HGT
25. UNDERSTANDING EVOLUTION BY USING
MOLECULAR FINGERPRINTING
Pre
genomic
era
• Pulsed Field Gel electrophoresis
• RAPD
• MLVA
Genomic
era
• Sanger Sequencing
• Pyrosequencing
Post
Genomic
era
• Second generation sequencing
(Illumina)
• Third Generation sequencing (Taqbio) 25
Ref- Rahman et al., 2015
26. FUTURE THREAT
Don’t ever think that I am
simple I can make big
TROUBLE!!
Strains of V. cholerae.
26
27. REFERENCES
Safa, A., Nair, G.B. and Kong, R.Y., 2010. Evolution of new variants
of Vibrio cholerae O1. Trends in microbiology, 18(1), pp.46-54.
Rahaman, M., Islam, T., Colwell, R.R. and Alam, M., 2015. Molecular
tools in understanding the evolution of Vibrio cholerae. Frontiers in
microbiology, 6, p.1040.
Kim, E.J., Lee, C.H., Nair, G.B. and Kim, D.W., 2015. Whole-genome
sequence comparisons reveal the evolution of Vibrio cholerae
O1. Trends in microbiology, 23(8), pp.479-489.
Mackay, D.M., 1980. Cholera: The present pandemic. Public
health, 94(5), pp.283-287.
Li, W., Raoult, D. and Fournier, P.E., 2009. Bacterial strain typing in
the genomic era. FEMS microbiology reviews, 33(5), pp.892-916.
27
28. REFERENCES
O'shea, Y.A., Reen, F.J., Quirke, A.M. and Boyd, E.F., 2004.
Evolutionary genetic analysis of the emergence of epidemic Vibrio
cholerae isolates on the basis of comparative nucleotide sequence
analysis and multilocus virulence gene profiles. Journal of clinical
microbiology, 42(10), pp.4657-4671.
Li, W., Raoult, D. and Fournier, P.E., 2009. Bacterial strain typing in
the genomic era. FEMS microbiology reviews, 33(5), pp.892-916.
Banerjee, R., Das, B., Nair, G.B. and Basak, S., 2014. Dynamics in
genome evolution of Vibrio cholerae. Infection, Genetics and
Evolution, 23, pp.32-41.
Reidl, J. and Klose, K.E., 2002. Vibrio cholerae and cholera: out of
the water and into the host. FEMS microbiology reviews, 26(2),
pp.125-139.
28