This document describes the solvent casting and particulate leaching method for fabricating scaffolds for tissue engineering. It involves mixing a biodegradable polymer solution with water soluble salt particles, casting the mixture into a mold of the desired shape, and then leaching out the salt particles with water to obtain a porous scaffold structure. The process leaves behind a porous scaffold structure that can be seeded with cells to support 3D tissue growth for medical purposes. Recent advances include using this method with selective laser sintering to 3D print scaffolds for applications such as craniofacial implants and cardiac tissue engineering.

1. VIGNESHWAR S
113017214051
BIOTECH 4th Year
SCAFFOLD MAKING
THANKS IN ADVANCE FOR YOUR KIND COOPERATION

2. SCAFFOLD
• Scaffolds are materials that have
been engineered to cause desirable
cellular interactions to contribute to the
formation of new functional tissues for
medical purposes.
• Cells are often 'seeded' into these
structures capable of supporting three-
dimensional tissue formation

4. SOLVENT CASTING AND PARTICULATE LEACHING
A simple and most commonly used method for fabricating scaffolds for tissue
engineering.
This method involves
1. Mixing water soluble salt (e.g., NaCl) particles into a biodegradable polymer solution.
2. The mixture is then casted into the mould of the desired shape.
3. After the solvent is removed by evaporation or lyophilisation, the salt particles are
leached out by water to obtain a porous structure

5. •A polymer is dissolved in an organic solvent.
•Particles, mainly salts, with specific dimensions are then added to the solution and the
mixture is shaped into its final geometry. For example, it can be cast onto a glass plate to
produce a membrane or in a three-dimensional mold to produce a scaffold.
•When the solvent evaporates, it creates a structure of composite material consisting of
the particles together with the polymer.
•The composite material is then placed in a bath which dissolves the particles, leaving
behind a porous structure

6. PROCESS

7. APPLICATION
It can be applied to any polymer that is soluble in a solvent
such as chloroform or methylene chloride
Advantages:
• Simple operation
• Adequate control of pore size and porosity by salt/polymer ratio and particle size of
the added salt, respectively
Limitations:
• Cubic Crystal Shape Of The Salt
• Thickness: 0.5‐2 mm
• Solvent Residue

8. Rapid prototyping is a group of techniques used to quickly fabricate a scale model of a
physical part or assembly using three-dimensional computer aided design (CAD) data.
Construction of the part or assembly is usually done using 3D printing or "additive layer
manufacturing" technology.

10. METHODOLOGY

12. •Recent advances in organ printing using SLS include 3D constructs
of craniofacial implants as well as scaffolds for cardiac tissue
engineering.
• Development enabling an organ recipient’s host cells to be used
to synthesize organs decreases the risk of organ rejection
•The ability to print organs has decreased the demand for animal
testing ranging from makeup to medical devices
Current Research

13. ANY QUERIES ?