The Role of Bioinformatics in The Drug Discovery Process, is an undergraduate seminar presentation in the department of Biochemistry, Faculty of life Sciences, University of Ilorin, Ilorin.
protein structure prediction methods. homology modelling, fold recognition, threading, ab initio methods. in short and easy form slides. after one time read you can easily understand methods for protein structure prediction.
Prediction of the three dimensional structure of a given protein sequence i.e. target protein from the amino acid sequence of a homologous (template) protein for which an X-ray or NMR structure is available based on an alignment to one or more known protein structures
INTRODUCTION
A PERFECT THERAPEUTIC DRUG
DRUG DISCOVERY- HISTORY
MODERN DRUG DISCOVERY
BIOINFORATICS IN DRUG DISCOVERY
DRUG DISCOVERY BASED ON BIOINFORMATIC TOOLS
BIOINFORMATICS IN COMPUTER-AIDED DRUG DISCOVERY
ECONOMICS OF DRUG DISCOVERY
CONCLUSION
REFERENCES
protein structure prediction methods. homology modelling, fold recognition, threading, ab initio methods. in short and easy form slides. after one time read you can easily understand methods for protein structure prediction.
Prediction of the three dimensional structure of a given protein sequence i.e. target protein from the amino acid sequence of a homologous (template) protein for which an X-ray or NMR structure is available based on an alignment to one or more known protein structures
INTRODUCTION
A PERFECT THERAPEUTIC DRUG
DRUG DISCOVERY- HISTORY
MODERN DRUG DISCOVERY
BIOINFORATICS IN DRUG DISCOVERY
DRUG DISCOVERY BASED ON BIOINFORMATIC TOOLS
BIOINFORMATICS IN COMPUTER-AIDED DRUG DISCOVERY
ECONOMICS OF DRUG DISCOVERY
CONCLUSION
REFERENCES
it will help you to understand how the protein microarrays are made, what are the different types and what all purposes they are used for. its very useful ppt
INTRODUCTION
DEFINITION OF BIOINFORMATICS
HISTORY
OBJECTIVES OF BIOINFORMATICS
TOOLS OF BIOINFORMATICS
BIOLOGICAL DATABASES
HOMOLOGY AND SIMILARITY TOOLS (SEQUENCE ALIGNMENT)
PROTEIN FUNCTION ANALYSIS TOOLS
STRUCTURAL ANALYSIS TOOLS
SEQUENCE MANIPULATION TOOLS
SEQUENCE ANALYSIS TOOLS
APPLICATION
CONCLUSION
REFERENCES
INTRODUCTION
WHAT IS DATA AND DATABASE?
WHAT IS BIOLOGICAL DATABASE?
TYPES OF BIOLOGICAL DATABASE
PRIMARY DATABASE
Nucleic acid sequence database
Protein sequence database
SECONDARY DATABASE
COMPOSITE DATABASE
TERTIARY DATABASE
WHY NEED?
CONCLUSION
REFRENCES
After sequencing of the genome has been done, the first thing that comes to mind is "Where are the genes?". Genome annotation is the process of attaching information to the biological sequences. It is an active area of research and it would help scientists a lot to undergo with their wet lab projects once they know the coding parts of a genome.
The Protein Data Bank (PDB) is a database for the three-dimensional structural data of large biological molecules, such as proteins and nucleic acids. This presentation deals with what, why, how, where and who of PDB. In this presentation we have also included briefing about various file formats available in PDB with emphasis on PDB file format
Ab Initio Protein Structure Prediction is a method to determine the tertiary structure of protein in the absence of experimentally solved structure of a similar/homologous protein. This method builds protein structure guided by energy function.
I had prepared this presentation for an internal project during my masters degree course.
INTRODUCTION.
NCBI.
EMBL.
DDBJ.
CONCLUSION.
REFERENSE.
The National Center for Biotechnology Information (NCBI) is part of the United States National Library of Medicine (NLM), a branch of the National Institutes of Health.
The NCBI is located in Bethesda, Maryland and was founded in 1988 through legislation sponsored by Senator Claude Pepper.
The NCBI houses a series of databases relevant to biotechnology and biomedicine. Major databases include GenBank for DNA sequences and PubMed, a bibliographic database for the biomedical literature.
All these databases are available online through the Entrez search engine.
Secondary Structure Prediction of proteins Vijay Hemmadi
Secondary structure prediction has been around for almost a quarter of a century. The early methods suffered from a lack of data. Predictions were performed on single sequences rather than families of homologous sequences, and there were relatively few known 3D structures from which to derive parameters. Probably the most famous early methods are those of Chou & Fasman, Garnier, Osguthorbe & Robson (GOR) and Lim. Although the authors originally claimed quite high accuracies (70-80 %), under careful examination, the methods were shown to be only between 56 and 60% accurate (see Kabsch & Sander, 1984 given below). An early problem in secondary structure prediction had been the inclusion of structures used to derive parameters in the set of structures used to assess the accuracy of the method.
Some good references on the subject:
A UX Journey into the World of Early Drug Discovery - UX Cambridge 2015Francis Rowland
Slides from a talk given by Francis Rowland and Niki Karamanis, at UX Cambridge 2015.
The main focus was the role of the UX designer as a catalyst in building a balanced team.
This was set in the context of a project that aims to integrate large quantities of complex data and provide an application that research scientists can use to aid the discovery of new medicines.
it will help you to understand how the protein microarrays are made, what are the different types and what all purposes they are used for. its very useful ppt
INTRODUCTION
DEFINITION OF BIOINFORMATICS
HISTORY
OBJECTIVES OF BIOINFORMATICS
TOOLS OF BIOINFORMATICS
BIOLOGICAL DATABASES
HOMOLOGY AND SIMILARITY TOOLS (SEQUENCE ALIGNMENT)
PROTEIN FUNCTION ANALYSIS TOOLS
STRUCTURAL ANALYSIS TOOLS
SEQUENCE MANIPULATION TOOLS
SEQUENCE ANALYSIS TOOLS
APPLICATION
CONCLUSION
REFERENCES
INTRODUCTION
WHAT IS DATA AND DATABASE?
WHAT IS BIOLOGICAL DATABASE?
TYPES OF BIOLOGICAL DATABASE
PRIMARY DATABASE
Nucleic acid sequence database
Protein sequence database
SECONDARY DATABASE
COMPOSITE DATABASE
TERTIARY DATABASE
WHY NEED?
CONCLUSION
REFRENCES
After sequencing of the genome has been done, the first thing that comes to mind is "Where are the genes?". Genome annotation is the process of attaching information to the biological sequences. It is an active area of research and it would help scientists a lot to undergo with their wet lab projects once they know the coding parts of a genome.
The Protein Data Bank (PDB) is a database for the three-dimensional structural data of large biological molecules, such as proteins and nucleic acids. This presentation deals with what, why, how, where and who of PDB. In this presentation we have also included briefing about various file formats available in PDB with emphasis on PDB file format
Ab Initio Protein Structure Prediction is a method to determine the tertiary structure of protein in the absence of experimentally solved structure of a similar/homologous protein. This method builds protein structure guided by energy function.
I had prepared this presentation for an internal project during my masters degree course.
INTRODUCTION.
NCBI.
EMBL.
DDBJ.
CONCLUSION.
REFERENSE.
The National Center for Biotechnology Information (NCBI) is part of the United States National Library of Medicine (NLM), a branch of the National Institutes of Health.
The NCBI is located in Bethesda, Maryland and was founded in 1988 through legislation sponsored by Senator Claude Pepper.
The NCBI houses a series of databases relevant to biotechnology and biomedicine. Major databases include GenBank for DNA sequences and PubMed, a bibliographic database for the biomedical literature.
All these databases are available online through the Entrez search engine.
Secondary Structure Prediction of proteins Vijay Hemmadi
Secondary structure prediction has been around for almost a quarter of a century. The early methods suffered from a lack of data. Predictions were performed on single sequences rather than families of homologous sequences, and there were relatively few known 3D structures from which to derive parameters. Probably the most famous early methods are those of Chou & Fasman, Garnier, Osguthorbe & Robson (GOR) and Lim. Although the authors originally claimed quite high accuracies (70-80 %), under careful examination, the methods were shown to be only between 56 and 60% accurate (see Kabsch & Sander, 1984 given below). An early problem in secondary structure prediction had been the inclusion of structures used to derive parameters in the set of structures used to assess the accuracy of the method.
Some good references on the subject:
A UX Journey into the World of Early Drug Discovery - UX Cambridge 2015Francis Rowland
Slides from a talk given by Francis Rowland and Niki Karamanis, at UX Cambridge 2015.
The main focus was the role of the UX designer as a catalyst in building a balanced team.
This was set in the context of a project that aims to integrate large quantities of complex data and provide an application that research scientists can use to aid the discovery of new medicines.
A UX Journey into the World of Early Drug DiscoveryJennifer Cham
Developing new medicines is an extremely challenging process with more than 50% of new medicines failing in late-stage development where the cost is the greatest. One of the main reasons for attrition is insufficient knowledge about the nature of the gene or protein (target) involved in a disease. Scientists in pharmaceutical research and development use diverse data and software applications to aid decision-making for drug target identification and validation.
We have been designing a new web portal to support researchers working within the pharmaceutical industry and academic organisations with the aim to make early drug target identification more efficient.
We will report on how we applied a range of participatory design methods including interviews, observations, sketching workshops, paper prototyping and usability testing to understand how experts carry out the very early stages of drug discovery. We will discuss the challenges of working in this domain and the extent to which standard UX approaches helped us understand what matters for our potential users so we could design and deliver solutions within an Agile framework. We also mention when popular UX methods didn't work in this complex environment and how we addressed these issues.
This work has been carried out via the Centre for Therapeutic Target Validation, a partnership between the European Bioinformatics Institute, the Wellcome Trust Sanger Institute and GlaxoSmithKline. See www.targetvalidation.org
DRUG DESIGN BASED ON BIOINFORMATICS TOOLSNIPER MOHALI
Drug design is a very complex process it takes many more times but using the these specific tools we can reduce complex process and save the time and produce a effective new drug that will be helpful in heath environment.
An Introduction to Bioinformatics
Drexel University INFO648-900-200915
A Presentation of Health Informatics Group 5
Cecilia Vernes
Joel Abueg
Kadodjomon Yeo
Sharon McDowell Hall
Terrence Hughes
Cytogenetic, Hematological and Enzymes Levels Parameters in the Biomonitoring...inventionjournals
Studies have demonstrated genotoxic effects by the presence of micronucleus in exfoliated cells from the buccal mucosa of agricultural workers exposed to pesticides. This study has assessed the genotoxic effects of pesticides on 61 agricultural workers from the state of Piauí, Brazil. 31 individuals were exposed to pesticides and 30 are from the same area, but were not involved in pesticides application. Cytogenetic damage were evaluated through micronucleus test in cells from the buccal mucosa and some parameters such as hematological and levels of enzymes. Exposed individuals exhibited cytogenetic damage with increased number of micronuclei in cells from the buccal mucosa in comparison with subjects from the control group with significant statistical difference (P < 0.01). We perceive that there is a statistically no significant (P > 0.05) increase in levels of plasmatic and eritrocytaireacetylcholinesterase and no statistically significant increase of phosphatase alkaline were detected in exposed workers in relation to the control group. No association was found in relation to smoking habits, alcohol consumption, protection utensils and the biomarkers analyzed or the biochemical analysis. Analysis of variance revealed a correlation between occupational exposure to pesticides of workers in Piauí and the presence of micronuclei (P < 0.05).
Cytogenetic, Hematological and Enzymes Levels Parameters in the Biomonitoring...inventionjournals
Studies have demonstrated genotoxic effects by the presence of micronucleus in exfoliated cells from the buccal mucosa of agricultural workers exposed to pesticides. This study has assessed the genotoxic effects of pesticides on 61 agricultural workers from the state of Piauí, Brazil. 31 individuals were exposed to pesticides and 30 are from the same area, but were not involved in pesticides application. Cytogenetic damage were evaluated through micronucleus test in cells from the buccal mucosa and some parameters such as hematological and levels of enzymes. Exposed individuals exhibited cytogenetic damage with increased number of micronuclei in cells from the buccal mucosa in comparison with subjects from the control group with significant statistical difference (P < 0.01). We perceive that there is a statistically no significant (P > 0.05) increase in levels of plasmatic and eritrocytaireacetylcholinesterase and no statistically significant increase of phosphatase alkaline were detected in exposed workers in relation to the control group. No association was found in relation to smoking habits, alcohol consumption, protection utensils and the biomarkers analyzed or the biochemical analysis. Analysis of variance revealed a correlation between occupational exposure to pesticides of workers in Piauí and the presence of micronuclei (P < 0.05).
Cytogenetic, Hematological and Enzymes Levels Parameters in the Biomonitoring...inventionjournals
Studies have demonstrated genotoxic effects by the presence of micronucleus in exfoliated cells from the buccal mucosa of agricultural workers exposed to pesticides. This study has assessed the genotoxic effects of pesticides on 61 agricultural workers from the state of Piauí, Brazil. 31 individuals were exposed to pesticides and 30 are from the same area, but were not involved in pesticides application. Cytogenetic damage were evaluated through micronucleus test in cells from the buccal mucosa and some parameters such as hematological and levels of enzymes. Exposed individuals exhibited cytogenetic damage with increased number of micronuclei in cells from the buccal mucosa in comparison with subjects from the control group with significant statistical difference (P < 0.01). We perceive that there is a statistically no significant (P > 0.05) increase in levels of plasmatic and eritrocytaireacetylcholinesterase and no statistically significant increase of phosphatase alkaline were detected in exposed workers in relation to the control group. No association was found in relation to smoking habits, alcohol consumption, protection utensils and the biomarkers analyzed or the biochemical analysis. Analysis of variance revealed a correlation between occupational exposure to pesticides of workers in Piauí and the presence of micronuclei (P < 0.05).
Drug response biomcare webinar - jan 2022Regin Jensen
WEBINAR: The role of the Gut Microbiome for Drug Response
Varying drug response is a key factor in both drug development and clinical practice and result in sub-optimal treatment and failed clinical trials.
Resent years research has detailed how the gut microbiome plays an essential role for drug response, and how the inter-individual variation in the composition of the gut microbiome is an important factor, both in drug trials and treatment.
Elements in the webinar
In this seminar, we will dive into this interesting topic, and take you through
-Key research into how the microbiome can affect drug response
-How microbiome profiling of patients can be used to gain insight and control in clinical trials at all stages,
-How microbiome profiling can be used to detect a novel type of biomarkers.
Biomcare is providing sampling support, sequencing, and data analysis for the microbiome aspects of the large NORDIC-SUN clinical trial of Immune Checkpoint Inhibitors, and we will finish the seminar by introducing this project and our solution for microbiome analysis in clinical studies and trials.
Studies show that about 20% of all recognized clinical pregnancies end in spontaneous abortion, mainly in the first trimester. Risk factors associated with the occurrence of a sporadic miscarriage have been established, with genetic factors being the most prevalent. As a problem that affects many couples, it is important to increase the quality of prognosis and diagnosis.
4th International Conference on Biomarkers & Clinical Research, will be organized around the theme "Impact of Biomarker Developments in Health Diagnostics and Clinical Research."
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Astronomy Update- Curiosity’s exploration of Mars _ Local Briefs _ leadertele...
The Role of Bioinformatics in The Drug Discovery Process
1. AN
UNDERGRADUATE SEMINAR
PRESENTATION
On
The Roles Of Bioinformatics in the Drug
Discovery Process
Department of Biochemistry,
Faculty of Life Sciences,
University of Ilorin, March 2015.
THEROLESOFBIOINFORMATICSINTHEDRUGDISCOVERYPROCESS
6/19/2015 3:19:54 PM
Department of Biochemistry, Faculty of Life Sciences, University of Ilorin, Ilorin.
2. THE ROLES OF BIOINFORMATICS
IN
THE DRUG DISCOVERY PROCESS
Presented
By
ADEBOWALE, Qazeem Omotola
11/55EH018
THEROLESOFBIOINFORMATICSINTHEDRUGDISCOVERYPROCESS
6/19/2015 3:19:54 PM
Department of Biochemistry, Faculty of Life Sciences, University of Ilorin, Ilorin.
4. THEROLESOFBIOINFORMATICSINTHEDRUGDISCOVERYPROCESS
Definition of Bioinformatics
As defined by National Center for
Biotechnology Information (2001).
As defined by Lopresti (2008).
Aims of Bioinformatics
To Store and arrange,
To analyze,
To interpret vast amount of large data
INTRODUCTION
6/19/2015 3:19:54 PM
Department of Biochemistry, Faculty of Life
Sciences, University of Ilorin, Ilorin.
6. THEROLESOFBIOINFORMATICSINTHEDRUGDISCOVERYPROCESS
THE DRUG DISCOVERY
6/19/2015 3:19:54 PM
Department of Biochemistry, Faculty of Life
Sciences, University of Ilorin, Ilorin.
What is Drug?
In pharmacology, Drug is any chemical
agent that alters the biochemical or
physiological processes of tissues or
organisms (United Nations Office Drug and
Crime, 2015 ).
8. THEROLESOFBIOINFORMATICSINTHEDRUGDISCOVERYPROCESS
THE DRUG DISCOVERY PROCESS
Developing a new drug (figure 2) is
Lengthy (Ibekwe and Ameh, 2014),
Risky
It involves a number of processes, that
are very expensive, figure 3, (Bie et al,
2015).
6/19/2015 3:19:54 PM
Department of Biochemistry, Faculty of Life
Sciences, University of Ilorin, Ilorin.
9. 6/19/2015 3:19:54 PM
Department of Biochemistry, Faculty of Life
Sciences, University of Ilorin, Ilorin.
Review and approval by Food &
Drug Administration
1 compound
approved
Phase III: Confirms effectiveness and monitors adverse
reactions from long-term use in 1,000 to
5,000 patient volunteers.
Phase II: Assesses effectiveness and looks for
side effects in 100 to 500 patient volunteers.
Phase I: Evaluates safety and dosage
in 20 to 100 healthy human volunteers.
5 compounds enter
clinical trials
Discovery and preclininal testing:
Compounds are identified and evaluated in
laboratory and animal studies for safety,
biological activity, and formulation.
5,000 compounds
evaluated
0 2 4 6 8 10 12 14 Years 16
Figure 2 : how risky and lengthy traditional drug discovery is (Source: Tufts
Center for the Study of Drug Development)
12. 6/19/2015 3:19:54 PM
Department of Biochemistry, Faculty of Life
Sciences, University of Ilorin, Ilorin.
Drug Discovery & Development
Identify disease
Isolate protein
involved in
disease (2-5 years)
Find a drug effective
against disease protein
(2-5 years)
Preclinical testing
(1-3 years)
Human clinical trials
(2-10 years)
FDA approval
(2-3 years)
Figure 4: Illustrating the stages of drug
discovery process (according to Tenthoff,
2006)
14. The application of bioinformatics cut
across all the process of drug discovery,
thereby
Reducing the risk of drug failure
Making it a bit cheaper
Reducing the time spent in the
discovery
And also automates the entire process,
thereby reducing human intervention.
THEROLESOFBIOINFORMATICSINTHEDRUGDISCOVERYPROCESS
6/19/2015 3:19:54 PM
Department of Biochemistry, Faculty of Life
Sciences, University of Ilorin, Ilorin.
ROLES OF BIOINFORMATICS IN THE DRUG DISCOVERY PROCESS
15. THEROLESOFBIOINFORMATICSINTHEDRUGDISCOVERYPROCESS
6/19/2015 3:19:54 PM
Department of Biochemistry, Faculty of Life
Sciences, University of Ilorin, Ilorin.
APPLICATIONS OF THE DRUG DISCOVERY PROCESS
IN TARGET IDENTIFICATION:
One need to know about the molecular bases
of the disease
Bioinformatics method have been used to
virtually screen target for compound that
binds and inhibit the protein (Searls , 2000).
16. IN TARGET VALIDATION
A target needs to be evaluated to ensure
that modulation of the target, will have
desired therapeutic effect.
This process can be accelerated with
bioinformatics tools, therefore speeds up
target validation step significantly (searls
, 2000).
THEROLESOFBIOINFORMATICSINTHEDRUGDISCOVERYPROCESS
6/19/2015 3:19:54 PM
Department of Biochemistry, Faculty of Life
Sciences, University of Ilorin, Ilorin.
APPLICATIONS OF THE DRUG DISCOVERY PROCESS
17. IN LEAD IDENTIFICATION
High throughput screening is a major
strategy for the discovery of new leads
(Evans 2009).
With Bioinformatics, protein targets are
screened against database of small
molecule compounds, to see which
molecule binds strongly to the targets.
THEROLESOFBIOINFORMATICSINTHEDRUGDISCOVERYPROCESS
6/19/2015 3:19:54 PM
Department of Biochemistry, Faculty of Life
Sciences, University of Ilorin, Ilorin.
18. IN LEAD OPTIMIZATION
The computational technique used in
refining the structure of lead compound is
QSAR - Quantity Structure Activity
Relationship (Roa and Srinivas 2011).
The information from QSAR can be used
to suggests new chemical modifications
for synthesis and testing (Roa and
Srinivas 2011).
THEROLESOFBIOINFORMATICSINTHEDRUGDISCOVERYPROCESS
6/19/2015 3:19:54 PM
Department of Biochemistry, Faculty of Life
Sciences, University of Ilorin, Ilorin.
19. IN PRE CLINICAL TESTING
Test is done with or without the use of
animals
Purpose: To limit the risk of novel
drugs
Preclinical testing involves:
Pharmacology,
Toxicology,
Pharmacokinetics, etc. (Roa and
Srinivas 2011).
THEROLESOFBIOINFORMATICSINTHEDRUGDISCOVERYPROCESS
6/19/2015 3:19:54 PM
Department of Biochemistry, Faculty of Life
Sciences, University of Ilorin, Ilorin.
20. ROLES IN CLINICAL TRIALS
The bioavailability and bioactivity of drugs are:
absorption, distribution, metabolism, excretion,
toxicity (ADMET) and efficacy (Roa and Srinivas
2011).
Although these properties are usually
measured in the lab, they can also be
predicted in advance with bioinformatics such
as C2-ADME, TOPKAT, CLOGP, DrugMatrix,
AbSolv, Bioprint, GastroPlus (Roa and Srinivas
2011).
THEROLESOFBIOINFORMATICSINTHEDRUGDISCOVERYPROCESS
6/19/2015 3:19:54 PM
Department of Biochemistry, Faculty of Life
Sciences, University of Ilorin, Ilorin.
21. Identify disease
Isolate protein
Find drug
Preclinical testing
GENOMICS, PROTEOMICS & BIOPHARM.
HIGH THROUGHPUT SCREENING
MOLECULAR MODELING
VIRTUAL SCREENING
COMBINATORIAL CHEMISTRY
IN VITRO & IN SILICO ADME MODELS
Potentially producing many more targets
and “personalized” targets
Screening up to 100,000 compounds a
day for activity against a target protein
Using a computer to
predict activity
Rapidly producing vast numbers
of compounds
Computer graphics & models help improve activity
Tissue and computer models begin to replace animal testing
Figure 5 : Summary of the application of bioinformatics in the
drug discovery process (According to Searls , 2000).
22. The drug discovery and development process is a long
and expensive one. It starts from target identification, to
Clinical trials before it is being approved.
Therefore before any newly discovered drug is placed
on the market, it must undergo extreme preclinical and
clinical tests and get a regulatory approval.
Due to the limitation of throughput, accuracy and cost,
experimental techniques cannot be applied widely, therefore,
in recent times the drug discovery process has shifted to the
use of BIOINFORMATICS.
Bioinformatics approach has been of great importance to
develop fast and accurate target identification and
prediction method for the discovery.
THEROLESOFBIOINFORMATICSINTHEDRUGDISCOVERYPROCESS
6/19/2015 3:19:54 PM
Department of Biochemistry, Faculty of Life
Sciences, University of Ilorin, Ilorin.
CONCLUSION