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Taxonomy
• Taxon = arrangement
• Classification,
• Nomenclature,
• Identification
Classification
•the arrangement of organisms into taxonomic groups
(taxon / taxa) on the basis of similarities or
relationships.
•Biochemical
•Physiologic
•Genetic
•morphologic
Nomenclature
• Naming an organism by international rules.
• International Committee on Systematic
Bacteriology
• Bergey’s Manual of systematic Bacteriology
Identification
• The practical use of a classification scheme
• Taxonomic Ranks
• Kingdom Prokaryotae
• Division Gracilicutes
• Class Scotobacteria
• Order Eubacteriales
• Family Enterobacteriaceae
• Genus Escherichia
• Species Escherichia coli
• Subtype Escherichia coli O157:H7
• Species
Sp.
Spp.
• Escherichia
• Escherichia coli
• E. coli
Classification
• Species
• group of individual isolates or strains
sharing a high degree of similarity in many
independent features
Criteria for Classification
• cell shape
• Specialized structures (spore, flagella)
• Staining ( Gram)
• Characteristic pigments
• Extracellular enzymes ( hemolysis )
• Characteristic metabolic functions (biochemical
tests)
• Sensitivity to antibiotics
• Molecular biology (comparing genes or genomes)
Numerical Taxonomy
• Computer taxonomy
• Phenetics
• Taxometrics
Phylogenetic Classification
• Common ancestor
• Close phylogenetic relatedness : recent
ancestor
• No fossil record exist for bacteria
• There is considerable genetic diversity
among bacteria
• G + C content
• DNA-DNA hybridization
• DNA sequencing
• Highly conserved genes
• Ribosome
• Ribosomal RNA
• Ribosomal proteins
• 16S rRNA
• Cells:
• Eukaryotes
• Prokaryotes
• Based on molecular phylogeny, prokaryotes
fall into two major groups.
• the Eubacteria
• the Archaebacteria,
• 3 Domains
• Bacteria
• Archaea
• Eucarya
• Eubacteria:
• Gram-negative eubacteria
• Gram-positive eubacteria
• Eubacteria lacking cell walls
• Progeny
• Clone
• Colony
• Epidemiology
• Outbreaks
• Clonality
• Practical purposes (genetically identical)
• Biotyping
• Serotyping
• Morphotyping
• Bacteriophage typing
• Bacteriocin typing
• Antimicrobial susceptibility testing
Subtyping
• Nonculturable pathogens
• Molecular analysis
(Taxonomy) mf

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