Streptomycin is an antibiotic primarily used for tuberculosis treatment, now often administered with other drugs due to its toxicity. It is produced through fermentation involving the microorganism Streptomyces griseus, which requires various substrates and growth conditions over three phases. Production typically employs a submerged culture method, with multiple recovery steps to purify the antibiotic.

1. STREPTOMYCIN BY- SANCHIT
DHANKHAR

2. IT IS AN ANTIBIOTIC THAT
WAS THE FIRST DRUG TO BE
SUCCESSFUL AGAINST
TUBERCULOSIS BUT NOW IT IS
USED WITH OTHER DRUGS
BECAUSE OF ITS TOXIC
EFFECTS

3. OTHER USES
Anti TB drug
Plaque
Veterinary treatment against gram negative bacteria in horses , cattle ,
sheep

4. ADMINISTRATION
Intravenously
Intramuscularly{ in many countries it
is only licensed to be used
intramuscularly}

5. FERMENTATION
Fermentation is defined as chemical transformation of organic compound brought
about through agency of microorganisms
Microorganisms useful in fementation can be either prokaryotes such as bacteria ,
virus or eukaryotes such as fungi; yeast

6. FERMENTATION OF
STREPTOMYCIN
Test Organism-Streptomyces griseus

7. REQUIREMENTS
Substrate-glucose, fructose, galactose, xylose, mannitol, lactose , starch
Source of nitrogen- peptone,meat extract, soy extract, the residues from alcohol
distillation, dried pencillium mycelium, ammonium salts,nitrates , glycine

8. Growth stimulating agents-phenyl acetic
acid, l – naphthalene
Antioxidant-starch , agar , sodium sulphite

9. 3. PHASES
first phase[2 days]
1. consist of mycelial growth
2. requirements
 oxygen, glucose, nitrogen, phosphorous
a. pH rises to 8.0 due to production of ammonia

10. SECOND PHASE(1 TO 2
DAYS)
In this streptomycin is produced without the production of mycelium
Large quantities of glucose and oxygen are required
Ammonia is utilized and ph falls to about 7.0

11. THIRD PHASE (1 TO 2
DAYS)
1
• Mycelium undergoes autolysis with the
rise in pH value
2
• pH falls again after a period of
continuous streptomycin production
3
• oxygen requirement is reduced after a
period of continuous streptomycin
production

12. TYPES OF CULTURE METHOD
IN FERMENTATION
1 Surface culture method in which mold is
grown on the surface of shallow layers of
fermentation medium
2 Submerged culture in which the mold is
grown submerged in fermentation medium in
shake flask or deep tanks

13. STREPTOMYCIN IS GENERALLY GROWN
BY SUBMERGED CULTURE METHOD BY
FOLLOWING WAYS
MASTER STOCK CULTURE – The selected production strain is maintained in the
form of master stock culture can be preserved by Freeze drying:
By fixing spores in sterilized soil sand mixtures
Storage under liquid nitrogen in frozen state
Second method is simple and adequate and simple to adopt

14. RECOVERY STEPS..
Master Culture
Agar Slopes
Shaker Flask
Seed Vessel
Fermenter
Filtration
Evaporator
Crystallization
Finishing

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