Disinfection of medical instruments is important for preventing the spread of disease. Cleaning and disinfecting reusable equipment after it comes into contact with patients can be expensive, both in the cost of the disinfection procedure, but also in terms of time away from the patients themselves.
Not all medical instruments can be fully sterilized after each use. Thankfully, not all reusable instruments need the highest level of disinfection. But how to determine the minimum level of disinfection in a given situation?
Earle H. Spaulding devised a rational approach to disinfection and sterilization of patient-care items and equipment
Spaulding believed the nature of disinfection could be understood readily if instruments and items for patient care were categorized as critical, semi-critical, and non-critical according to the degree of risk for infection involved in use of the items.
Spaulding recognized that the need for disinfection of equipment in medical settings ranged from non-critical to semi-critical to critical, depending on the likelihood of spreading disease.
For example, sterilization is necessary for equipment that comes into contact with a patient’s bloodstream or sterile tissue. This category of equipment, such as surgical knives, is designated “critical” because it presents a high risk of disease transmission from patient to patient.
Equipment that only touches healthy, unbroken skin presents a low risk of contamination because intact skin acts as an effective barrier to most microorganisms. Examples in this “non-critical” category include bedpans, blood-pressure cuffs, and bedrails.
In between those two scenarios, a “semi-critical” level of disinfection presents a medium risk of contamination. This would include equipment such as endoscopes used on mucous membranes or areas of broken skin.
Infection control prevents or stops the spread of infections in healthcare settings
sterilization is a process which kills all forms of microbial life including transmissible agents such as virus, bacteria, fungi and spore forms
disinfection is define as a destruction or inhibition of most pathogenic agent on the surface of inanimate object by chemical or physical means.
Methods of Handwashing are
A.Short Scrub
B. Short Standard Handwash
C. Surgical Hand Scrub
Rationale
Chain of infection
Routes of disease transmission
CDC and OSHA
Spauldings classification
Sterilization protocol
Methods of sterilization-physical and chemical agents
New methods of sterilization
Sterilization of scaler handpeice and inserts
Infection control
Infectious diseases commonly encounterd in dentistry
Medical history and dental safety
Immunization of personnel involved in dental care
Infection control practices
Hand hygiene
Personal protective equipments
Surface barriers
Waste management in dental practice
Cdc guidelines-special considerations
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and
offering a wide range of dental certified courses in different formats.for more details please visit
www.indiandentalacademy.com
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and
offering a wide range of dental certified courses in different formats.for more details please visit
www.indiandentalacademy.com
Infection control prevents or stops the spread of infections in healthcare settings
sterilization is a process which kills all forms of microbial life including transmissible agents such as virus, bacteria, fungi and spore forms
disinfection is define as a destruction or inhibition of most pathogenic agent on the surface of inanimate object by chemical or physical means.
Methods of Handwashing are
A.Short Scrub
B. Short Standard Handwash
C. Surgical Hand Scrub
Rationale
Chain of infection
Routes of disease transmission
CDC and OSHA
Spauldings classification
Sterilization protocol
Methods of sterilization-physical and chemical agents
New methods of sterilization
Sterilization of scaler handpeice and inserts
Infection control
Infectious diseases commonly encounterd in dentistry
Medical history and dental safety
Immunization of personnel involved in dental care
Infection control practices
Hand hygiene
Personal protective equipments
Surface barriers
Waste management in dental practice
Cdc guidelines-special considerations
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and
offering a wide range of dental certified courses in different formats.for more details please visit
www.indiandentalacademy.com
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and
offering a wide range of dental certified courses in different formats.for more details please visit
www.indiandentalacademy.com
Sterilization /certified fixed orthodontic courses by Indian dental academy Indian dental academy
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and offering a wide range of dental certified courses in different formats.
Indian dental academy provides dental crown & Bridge,rotary endodontics,fixed orthodontics,
Dental implants courses.for details pls visit www.indiandentalacademy.com ,or call
0091-9248678078
Sterilization and disinfection of orthodontic instruments /certified fixed or...Indian dental academy
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and offering a wide range of dental certified courses in different formats.
Indian dental academy provides dental crown & Bridge,rotary endodontics,fixed orthodontics,
Dental implants courses.for details pls visit www.indiandentalacademy.com ,or call
0091-9248678078
There are nearly 100 viruses of the herpes group that infect many different animal species.
Official name of herpesviruses that commonly infect human is Humans herpesvirus (HHV)
herpes simplex virus types 1 (HHV 1)
Herpes simplex virus type 2 (HHV 2)
Varicella-zoster virus (HHV 3)
Epstein-Barr virus, (HHV 4)
Cytomegalovirus (HHV 5)
Human herpesvirus 6 (HHV 6)
Human herpesvirus 7 (HHV 7)
Human herpesvirus 8 (HHV 8) (Kaposi's sarcoma-associated herpesvirus).
Herpes B virus of monkeys can also infect humans
hELMINTHS#corona virus#Aspergillosis#BUGANDO#CUHAS#CUHAS#CUHAS
The very first requirement in a hospital that it should do the sick no harm" - Florence Nightingale
Health care associated infections economic loss, prolonged hospital stay & adverse patient outcomes.
Herpes viruses ppt prepared by DR. PRINCE C PDR.PRINCE C P
PPT prepared by DR.PRINCE C P
Associate Professor
Department of Microbiology
Mother Theresa Post Graduate & Research Institute of Health Sciences (Government of Puducherry Institution)
Herpes viruses
HSV type 1
HSV type 2
Varicella - Zoster virus
EB viruses
Cytomegalovirus
Polio viruses and polio immunisation ppt by Dr Prince C PDR.PRINCE C P
The causative agent of poliomyelitis (commonly known as polio), is a human Enterovirus and member of the family of Picornaviridae.
Poliovirus was first isolated in 1909 by Karl Landsteiner and Erwin Popper.
Poliovirus is one of the most well-characterized viruses, and has become a useful model system for understanding the biology of RNA viruses.
A breakthrough came in 1948 when the virus was successfully cultivated in human tissue in the laboratory by John Enders.
Enders, Weller and Robins, passaged the same strain in non neuronal cell culture.
Vaccines against poliomyelitis: the formalin-inactivated vaccine (IPV) by Jonas Salk(1953) and the live-attenuated vaccines (OPV) by Albert Sabin (1956)
More Related Content
Similar to Spauldings classification ppt by Dr C P PRINCE
Sterilization /certified fixed orthodontic courses by Indian dental academy Indian dental academy
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and offering a wide range of dental certified courses in different formats.
Indian dental academy provides dental crown & Bridge,rotary endodontics,fixed orthodontics,
Dental implants courses.for details pls visit www.indiandentalacademy.com ,or call
0091-9248678078
Sterilization and disinfection of orthodontic instruments /certified fixed or...Indian dental academy
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and offering a wide range of dental certified courses in different formats.
Indian dental academy provides dental crown & Bridge,rotary endodontics,fixed orthodontics,
Dental implants courses.for details pls visit www.indiandentalacademy.com ,or call
0091-9248678078
There are nearly 100 viruses of the herpes group that infect many different animal species.
Official name of herpesviruses that commonly infect human is Humans herpesvirus (HHV)
herpes simplex virus types 1 (HHV 1)
Herpes simplex virus type 2 (HHV 2)
Varicella-zoster virus (HHV 3)
Epstein-Barr virus, (HHV 4)
Cytomegalovirus (HHV 5)
Human herpesvirus 6 (HHV 6)
Human herpesvirus 7 (HHV 7)
Human herpesvirus 8 (HHV 8) (Kaposi's sarcoma-associated herpesvirus).
Herpes B virus of monkeys can also infect humans
hELMINTHS#corona virus#Aspergillosis#BUGANDO#CUHAS#CUHAS#CUHAS
The very first requirement in a hospital that it should do the sick no harm" - Florence Nightingale
Health care associated infections economic loss, prolonged hospital stay & adverse patient outcomes.
Similar to Spauldings classification ppt by Dr C P PRINCE (20)
Herpes viruses ppt prepared by DR. PRINCE C PDR.PRINCE C P
PPT prepared by DR.PRINCE C P
Associate Professor
Department of Microbiology
Mother Theresa Post Graduate & Research Institute of Health Sciences (Government of Puducherry Institution)
Herpes viruses
HSV type 1
HSV type 2
Varicella - Zoster virus
EB viruses
Cytomegalovirus
Polio viruses and polio immunisation ppt by Dr Prince C PDR.PRINCE C P
The causative agent of poliomyelitis (commonly known as polio), is a human Enterovirus and member of the family of Picornaviridae.
Poliovirus was first isolated in 1909 by Karl Landsteiner and Erwin Popper.
Poliovirus is one of the most well-characterized viruses, and has become a useful model system for understanding the biology of RNA viruses.
A breakthrough came in 1948 when the virus was successfully cultivated in human tissue in the laboratory by John Enders.
Enders, Weller and Robins, passaged the same strain in non neuronal cell culture.
Vaccines against poliomyelitis: the formalin-inactivated vaccine (IPV) by Jonas Salk(1953) and the live-attenuated vaccines (OPV) by Albert Sabin (1956)
Botulism/ Clostridium botulinum.ppt prepared by Dr PRINCE C P.DR.PRINCE C P
Botulism has been used as a weapon of terror in the past. During World War II, the infamous Unit 731, the Japanese biological warfare group, fed cultures of Clostridium botulinum to prisoners during the Japanese occupation of Manchuria, killing them.
It was suspected that Germany had weaponized botulism as well, and several other countries were suspected to have done research into the use of botulism as a weapon.
More recently, between 1990 and 1995, aerosols were dispersed on at least three occasions by the Japanese cult Aum Shinrikyo, who would later go on to release sarin gas in the Tokyo subway, to disastrous effect. These releases of botulism failed for various reasons, but the ease with which Aum Shinrikyo was able to culture Clostridium botulinum from the soil is one reason that this toxin would make an excellent bioweapon.
Higher education for Science students by Dr. PRINCE C PDR.PRINCE C P
Higher Education opportunities and career pathways forScience students.Paramedical courses refer to educational programs that prepare individuals to work in various healthcare settings alongside medical professionals, providing essential support services. Paramedical courses equip students with the necessary knowledge and skills to assist in patient care, diagnostics, rehabilitation, and other healthcare-related tasks. The graduates of Paramedical courses play a crucial role in the healthcare industry by complementing the work of doctors, nurses, and other healthcare professionals.
Emergence of Drug resistant microbes PPT By DR.C.P.PrinceDR.PRINCE C P
Antimicrobial resistance is resistance of a microorganism to an antimicrobial drug that was originally effective for treatment of infections caused by it.
Resistant microorganisms (including bacteria, fungi, viruses and parasites) are able to withstand attack by antimicrobial drugs, such as antibacterial drugs (e.g. antibiotics), antifungals, antivirals, and antimalarials, so that standard treatments become ineffective and infections persist, increasing the risk of spread to others.
Biomarkers for early diagnosis ppt by Dr C P PRINCEDR.PRINCE C P
A biomarker is a characteristic ‘substance’, analyte, or otherwise a ‘thing’ that can be objectively measured as an indicator of normal biological processes, pathogenic processes or a pharmacological response to a therapeutic intervention. These are used for many purposes including disease diagnosis and prognosis, prediction and assessment of treatment response.
Biomarkers can be characteristic biological properties or molecules that can be detected and measured in parts of the body like the blood or tissue. They may indicate either normal or diseased processes in the body.
Biomarkers can be specific cells, molecules, or genes, gene products, enzymes, or hormones.
VIRUSES structure and classification ppt by Dr.Prince C PDR.PRINCE C P
Small size (20-300 nm) / bacteria (size measured in μm)
Viral genome – single type of nucleic acid:
DNA – dezoxyriboviruses
RNA - riboviruses
Totally dependant on living cells for all metabolic processes
May infect humans, animals, plants, bacteria, fungi,parasites, insects
Virion = viral corpuscle = elementary, infectious unit.
Only visible by electron microscopy.
Laboratory diagnosis of viral infections
Methods:
• Cytology
• Electron microscopy
• Cultivation
• Detection of viral proteins
• Serology
• Molecular diagnosis (detection of
genetic material i.e. nucleic acids)
PPT prepared by Dr. Prince C P
Protozoa and Helminth Parasites ppt by Dr.Prince.C.PDR.PRINCE C P
PPT prepared by :Dr.Prince.C.P
Associate Professor & HOD , Department of Microbiology,
Mother Theresa Post Graduate & Research Institute of Health Sciences (Government of Puducherry Institution)
Medical Parasitology is the subject which deals with the parasites that infect human being, the diseases caused by them, clinical feature and the response generated by human being against them. It's also concerned with the various methods of their diagnosis, treatment and finally their prevention & control.
An ova or cyst or egg is detected by microscopic evaluation of a stool sample that is used to look for parasites that may infect the lower digestive tract, causing symptoms such as diarrhoea. The parasites and their eggs (ova) are shed from the lower digestive tract into the stool
Stool examination (Microscopic) is performed for the diagnosis of following parasitic infections
1. Protozoa • Entamoeba histolytica • Giardia lamblia • intestinal coccidian parasites (i) Cryptosporidium parvum (ii) Cyclospora (iii) Isospora • Balantidium coli
2. Helminthes • nematodes: (i) Ascaris lumbricoides (ii) Trichuris trichuria
(Iii) hookworm • Ancylostoma duodenale • Nectar americans (iv) Strongyloides stercoralis
Cestodes: (i) Taenia spp • T. Saginata • T.Solium (ii) Hymenolepsis nana (iii) Enterobius vermicularis
Fungal Infections/ Mycoses ppt by Dr.C.P.PRINCEDR.PRINCE C P
PPT prepared by :
DR.PRINCE C P
Associate Professor &HOD
Department of Microbiology,
Mother Theresa Post Graduate & Research Institute of Health Sciences (Government of Puducherry Institution)
According to tissue involved, MYCOSES are classified into:
Superficial (Surface )
Cutaneous
Subcutaneous
Deep Cutaneous
Systemic (Primary )
Systemic ( Opportunistic)
Mycotic Poisoning
most of the fungal infections are opportunistic in nature.
candida albicans is the common Fungal pathogen.
CSSD- Central Sterile Supply Department ppt by DR.PRINCE.C.PDR.PRINCE C P
Sterile supplies to all departments , both to specialized units as well as general wards and OPDs.
To provide sterilized material from a central department where sterilizing process is carried out under properly controlled conditions
To alleviate the burden of work of the nursing personnel, there by enabling them to devote more of their time to patient care .
Assurance of adequate supply of sterile products immediately and constantly available for sometime as well as emergency use.
prepared by:
DR PRINCE C P
Associate Professor in Microbiology
Mother Teresa PG& RI of Health Sciences
Puducherry
Autoimmunity & disorders ppt by DR.C P. PRINCEDR.PRINCE C P
PPT prepared by :
DR.PRINCE C P
HOD & Associate Professor
Department of Microbiology,
Mother Theresa
Post Graduate & Research Institute of Health Sciences (Government of Puducherry Institution)
Pondicherry.
An autoimmune disorder is a condition that occurs when the immune system attacks and destroys healthy body tissue.
Graves’ disease
Hashimoto’s thyroiditis
Addison’s disease
Myasthenia gravis
SLE
Diabetes mellitus type
Hypersensitivity/ Allergy ppt by DR.C.P.PRINCEDR.PRINCE C P
Hypersensitivity refers to undesirable reactions produced by the normal immune system, including allergies .
These reactions may be damaging, uncomfortable, or occasionally fatal.
ALLERGEN: non-parasite antigens that can stimulate a hypersensitivity response
Occurs in two stages : sensitization phase and shocking phase
Classified into Immediate and Delayed hypersensitivity based on time required to develop the symptoms
Type I hypersensitivity is also known as immediate or anaphylactic hypersensitivity.
The reaction may involve skin(urticaria and eczema), eyes(conjunctivitis), nasopharynx (rhinorrhea, rhinitis), bronchopulmonary tissues(asthma) and gastrointestinal tract (gastroenteritis).
Antigen ,Antibody and Ag-Ab reactions ppt by DR.C.P.PRINCEDR.PRINCE C P
An immunogen refers to a molecule that is capable of eliciting an immune response, whereas an antigen refers to a molecule that is capable of binding to the product of that immune response (Ab).
So, an immunogen is necessarily an antigen, but an antigen may not necessarily be an immunogen
The terms immunogen and antigen are often used interchangeably but the later is more common.
Antibodies are Globulin Protein (Immunoglobulin) that are synthesized in the Serum and Tissue fluids.
It reacts specifically with the antigen that stimulated their production.
There are two types serum proteins: albumin and globulin
There are Three types of globulins .
1. Alpha globulin
2. Beta globulin
3. Gamma globulin (Antibodies)
Gamma globulins are responsible for immunity. So they are called as Immunoglobulin (Ig)
The binding of an antibody with an antigen of the type that stimulated the formation of antibody that results in the following reaction
Agglutination
Precipitation
Complement fixation
Phagocytosis
Neutralization of an exotoxin
Opsonization
Tissue fixation
Chemotaxis
Activation of mast cells and basophils
PPT prepared by:
DR.PRINCE C P
Associate Professor , Department of Microbiology,
Mother Theresa Post Graduate & Research Institute of Health Sciences (Government of Puducherry Institution)
Immune system and immunity ppt by DR.C.P.PRINCEDR.PRINCE C P
Immunity is the power to resist and overcome infection caused by particular organism.
RESISTANCE EXHIBITED BY THE HOST AGAINST MICROBES AND THEIR PRODUCTS
Innate immunity:“Innate” because shared by all animals (Pre-existing/ By birth) and Non-specific
Adaptive immunity (Acquired Immunity):Responsive and Specific
The immune system recognizes, attacks, destroys, and remembers each pathogen that enters the body.
The Immune System includes all parts of the body that help in the recognition and destruction of foreign materials.
White blood cells, phagocytes and lymphocytes, bone marrow, lymph nodes, tonsils, thymus, and your spleen are all part of the immune system.
prepared by:
DR.PRINCE C P
HOD & Associate Professor
Department of Microbiology
Mother Theresa Post Graduate & Research Institute of Health Sciences (Government of Puducherry Institution)
Pondicherry
STERILITY TESTING OF PHARMACEUTICALS ppt by DR.C.P.PRINCEDR.PRINCE C P
Sterility Testing: done to detect if viable forms of micro-organisms are present or not on or in the pharmaceutical preparation.
The test is applied to substances or preparations which, according to the Pharmacopoeia, are required to be sterile. For example
✦ Injections
✦ Implants
✦ Syringes
✦ Bandages
✦ Dressings
✦ Surgical Instruments
✦ Needles
✦ Injectables
✦ Bulk Solids
✦ Ophthalmic Products..etc
If microorganisms are placed in a media that provides nutrients and water and kept at a favourable temperature the organism will grow and their growth can be indicated by turbidity in originally clear medium.
PPT prepared by:
DR.PRINCE C P
HOD &Associate Professor
Department of Microbiology,
Mother Theresa Post Graduate & Research Institute of Health Sciences (Government of Puducherry Institution)
Introduction to Microbiology & Microorganisms PPT by DR.C.P.PinceDR.PRINCE C P
Microorganisms are the minute living bodies not visible to the naked eyes.Example: Bacteria, Yeast, Amoeba etc
Microbiology is the specific branch of ‘biology’ that deals with the elaborated investigation of ‘small organisms’ termed as microbes or Microorganisms.
In simple words, Microbiology is the study of microorganisms.
Bacteria Example: Anthrax Bacilli, Esch. Coli
Fungi - .Example: Yeast, Mushrooms, Pencillium
Algae Example: Diatoms
Protozoan Example : Malaria parasite
Viruses - Example:HIV,Chicken pox
Worms – Example: Round worm, Pin worm
PPT prepared by :
DR.C.P.PRINCE
Bacterial growth curve ppt by Dr.C.P.PRINCEDR.PRINCE C P
Bacteria multiply by binary fission. The growth bacteria is measured in terms of the number of cells . total count and viable count.
osmotic environment can affect bacterial growth.
Bacteria can be grouped according to their energy source as phototrophs or chemotrophs.
Bacteria can be grouped according to their carbon source as autotrophs or heterotrophs.
Combining their nutritional patterns, all organisms in nature can be placed into one of four separate groups: photoautotrophs, photoheterotrophs, chemoautotrophs, and chemoheterotrophs.
Bacteria also need a nitrogen source, various minerals, and water for growth.
Organisms having complex nutritional requirements and needing many growth factors are said to be fastidious.
PPT prepared by:
DR.PRINCE C P
HOD & Associate Professor
Department of Microbiology
Mother Theresa Post Graduate & Research Institute of Health Sciences (Government of Puducherry Institution)
Pondicherry
Culture media and Cultivation of Bacteria DR.C.P.PRINCEDR.PRINCE C P
Purpose of culturing are
Isolation of bacteria ( pure culture)
Diagnosis of infectious diseases
Properties of bacteria i.e. culturing bacteria is the initial step in studying its morphology and its identification.
Maintenance of stock cultures.
Estimate viable counts. Water , air, milk testing
To test for antibiotic sensitivity.
To create antigens for laboratory use.
Vaccine preparation
Sterility testing
Preparation of pharmaceutical products like antibiotics, enzymes, toxins etc
Certain genetic studies and manipulations of the cells also need that bacteria to be cultured in vitro.
Culturing on solid media is another convenient way of separating bacteria in mixture.
An artificial culture media must provide similar environmental and nutritional conditions that exist in the natural habitat of a bacterium.
A culture medium contains water, a source of carbon & energy, source of nitrogen, trace elements and some growth factors.
PPT prepared by:
DR.C. P. PRINCE
HOD & Associate Professor
Department of Microbiology
Mother Theresa Post Graduate & Research Institute of Health Sciences (Government of Puducherry Institution)
Antibiotic sensitivity test PPT by Dr.C.P.PRINCEDR.PRINCE C P
Antibiotic sensitivity test: in vitro testing of bacterial cultures with antibiotics to determine susceptibility of bacteria to antibiotic therapy.
A laboratory test which determines how effective antibiotic therapy is against a bacterial infections.
Antibiotic sensitivity testing will control the use of Antibiotics in clinical practice
Testing will assist the clinicians in the choice of drugs for the treatment of infections.
Helps to guide the Physician in choosing Antibiotics
The accumulated results on different pathogens their sensitivity will guide the physician in choosing empirical treatment in serious patients before the individual’s laboratory results are analyzed in the Microbiology laboratory.
Reveals the changing trends in the local isolates.
Helps the local pattern of antibiotic prescribing.
PPT Prepared by
Dr.Prince.C.P
Department of Microbiology
Mother Theresa PG&RIHS
Pondicherry
Classification and mechanism of action of ANTIMICROBIALS by DR.PRINCE.C.PDR.PRINCE C P
An antibiotic is a selective poison.
It has been chosen so that it will kill the desired bacteria, but not the cells in your body. Each different type of antibiotic affects different bacteria in different ways.
For example, an antibiotic might inhibit a bacteria's ability to turn glucose into energy, or the bacteria's ability to construct its cell wall.
Therefore the bacteria dies instead of reproducing.
this ppt on antimicrobials is a brief description of antimicrobials used for treatment .
by
Dr.C.P.PRINCE
Pondicherry
One of the most developed cities of India, the city of Chennai is the capital of Tamilnadu and many people from different parts of India come here to earn their bread and butter. Being a metropolitan, the city is filled with towering building and beaches but the sad part as with almost every Indian city
We understand the unique challenges pickleball players face and are committed to helping you stay healthy and active. In this presentation, we’ll explore the three most common pickleball injuries and provide strategies for prevention and treatment.
Antibiotic Stewardship by Anushri Srivastava.pptxAnushriSrivastav
Stewardship is the act of taking good care of something.
Antimicrobial stewardship is a coordinated program that promotes the appropriate use of antimicrobials (including antibiotics), improves patient outcomes, reduces microbial resistance, and decreases the spread of infections caused by multidrug-resistant organisms.
WHO launched the Global Antimicrobial Resistance and Use Surveillance System (GLASS) in 2015 to fill knowledge gaps and inform strategies at all levels.
ACCORDING TO apic.org,
Antimicrobial stewardship is a coordinated program that promotes the appropriate use of antimicrobials (including antibiotics), improves patient outcomes, reduces microbial resistance, and decreases the spread of infections caused by multidrug-resistant organisms.
ACCORDING TO pewtrusts.org,
Antibiotic stewardship refers to efforts in doctors’ offices, hospitals, long term care facilities, and other health care settings to ensure that antibiotics are used only when necessary and appropriate
According to WHO,
Antimicrobial stewardship is a systematic approach to educate and support health care professionals to follow evidence-based guidelines for prescribing and administering antimicrobials
In 1996, John McGowan and Dale Gerding first applied the term antimicrobial stewardship, where they suggested a causal association between antimicrobial agent use and resistance. They also focused on the urgency of large-scale controlled trials of antimicrobial-use regulation employing sophisticated epidemiologic methods, molecular typing, and precise resistance mechanism analysis.
Antimicrobial Stewardship(AMS) refers to the optimal selection, dosing, and duration of antimicrobial treatment resulting in the best clinical outcome with minimal side effects to the patients and minimal impact on subsequent resistance.
According to the 2019 report, in the US, more than 2.8 million antibiotic-resistant infections occur each year, and more than 35000 people die. In addition to this, it also mentioned that 223,900 cases of Clostridoides difficile occurred in 2017, of which 12800 people died. The report did not include viruses or parasites
VISION
Being proactive
Supporting optimal animal and human health
Exploring ways to reduce overall use of antimicrobials
Using the drugs that prevent and treat disease by killing microscopic organisms in a responsible way
GOAL
to prevent the generation and spread of antimicrobial resistance (AMR). Doing so will preserve the effectiveness of these drugs in animals and humans for years to come.
being to preserve human and animal health and the effectiveness of antimicrobial medications.
to implement a multidisciplinary approach in assembling a stewardship team to include an infectious disease physician, a clinical pharmacist with infectious diseases training, infection preventionist, and a close collaboration with the staff in the clinical microbiology laboratory
to prevent antimicrobial overuse, misuse and abuse.
to minimize the developme
CRISPR-Cas9, a revolutionary gene-editing tool, holds immense potential to reshape medicine, agriculture, and our understanding of life. But like any powerful tool, it comes with ethical considerations.
Unveiling CRISPR: This naturally occurring bacterial defense system (crRNA & Cas9 protein) fights viruses. Scientists repurposed it for precise gene editing (correction, deletion, insertion) by targeting specific DNA sequences.
The Promise: CRISPR offers exciting possibilities:
Gene Therapy: Correcting genetic diseases like cystic fibrosis.
Agriculture: Engineering crops resistant to pests and harsh environments.
Research: Studying gene function to unlock new knowledge.
The Peril: Ethical concerns demand attention:
Off-target Effects: Unintended DNA edits can have unforeseen consequences.
Eugenics: Misusing CRISPR for designer babies raises social and ethical questions.
Equity: High costs could limit access to this potentially life-saving technology.
The Path Forward: Responsible development is crucial:
International Collaboration: Clear guidelines are needed for research and human trials.
Public Education: Open discussions ensure informed decisions about CRISPR.
Prioritize Safety and Ethics: Safety and ethical principles must be paramount.
CRISPR offers a powerful tool for a better future, but responsible development and addressing ethical concerns are essential. By prioritizing safety, fostering open dialogue, and ensuring equitable access, we can harness CRISPR's power for the benefit of all. (2998 characters)
India Clinical Trials Market: Industry Size and Growth Trends [2030] Analyzed...Kumar Satyam
According to TechSci Research report, "India Clinical Trials Market- By Region, Competition, Forecast & Opportunities, 2030F," the India Clinical Trials Market was valued at USD 2.05 billion in 2024 and is projected to grow at a compound annual growth rate (CAGR) of 8.64% through 2030. The market is driven by a variety of factors, making India an attractive destination for pharmaceutical companies and researchers. India's vast and diverse patient population, cost-effective operational environment, and a large pool of skilled medical professionals contribute significantly to the market's growth. Additionally, increasing government support in streamlining regulations and the growing prevalence of lifestyle diseases further propel the clinical trials market.
Growing Prevalence of Lifestyle Diseases
The rising incidence of lifestyle diseases such as diabetes, cardiovascular diseases, and cancer is a major trend driving the clinical trials market in India. These conditions necessitate the development and testing of new treatment methods, creating a robust demand for clinical trials. The increasing burden of these diseases highlights the need for innovative therapies and underscores the importance of India as a key player in global clinical research.
How many patients does case series should have In comparison to case reports.pdfpubrica101
Pubrica’s team of researchers and writers create scientific and medical research articles, which may be important resources for authors and practitioners. Pubrica medical writers assist you in creating and revising the introduction by alerting the reader to gaps in the chosen study subject. Our professionals understand the order in which the hypothesis topic is followed by the broad subject, the issue, and the backdrop.
https://pubrica.com/academy/case-study-or-series/how-many-patients-does-case-series-should-have-in-comparison-to-case-reports/
The dimensions of healthcare quality refer to various attributes or aspects that define the standard of healthcare services. These dimensions are used to evaluate, measure, and improve the quality of care provided to patients. A comprehensive understanding of these dimensions ensures that healthcare systems can address various aspects of patient care effectively and holistically. Dimensions of Healthcare Quality and Performance of care include the following; Appropriateness, Availability, Competence, Continuity, Effectiveness, Efficiency, Efficacy, Prevention, Respect and Care, Safety as well as Timeliness.
QA Paediatric dentistry department, Hospital Melaka 2020Azreen Aj
QA study - To improve the 6th monthly recall rate post-comprehensive dental treatment under general anaesthesia in paediatric dentistry department, Hospital Melaka
Leading the Way in Nephrology: Dr. David Greene's Work with Stem Cells for Ki...Dr. David Greene Arizona
As we watch Dr. Greene's continued efforts and research in Arizona, it's clear that stem cell therapy holds a promising key to unlocking new doors in the treatment of kidney disease. With each study and trial, we step closer to a world where kidney disease is no longer a life sentence but a treatable condition, thanks to pioneers like Dr. David Greene.
1. Spaulding Classification
DR.PRINCE C P
HOD & Associate Professor , Department of Microbiology,
Mother Theresa Post Graduate & Research Institute of Health Sciences
(Government of Puducherry Institution)
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4. Introduction
• Disinfection of medical instruments is important for preventing the
spread of disease. Cleaning and disinfecting reusable equipment after
it comes into contact with patients can be expensive, both in the cost
of the disinfection procedure, but also in terms of time away from the
patients themselves.
• Not all medical instruments can be fully sterilized after each use.
Thankfully, not all reusable instruments need the highest level of
disinfection. But how to determine the minimum level of disinfection
in a given situation?
5. Guideline for Disinfection and Sterilization in
Healthcare Facilities
• Earle H. Spaulding devised a rational approach to disinfection and
sterilization of patient-care items and equipment
• Spaulding believed the nature of disinfection could be understood
readily if instruments and items for patient care were categorized as
critical, semi-critical, and non-critical according to the degree of risk
for infection involved in use of the items.
6. Overview of the Spaulding Classification
• Spaulding recognized that the need for disinfection of equipment in
medical settings ranged from non-critical to semi-critical to critical,
depending on the likelihood of spreading disease.
• For example, sterilization is necessary for equipment that comes into
contact with a patient’s bloodstream or sterile tissue. This category of
equipment, such as surgical knives, is designated “critical” because it
presents a high risk of disease transmission from patient to patient.
• Equipment that only touches healthy, unbroken skin presents a low risk of
contamination because intact skin acts as an effective barrier to most
microorganisms. Examples in this “non-critical” category include bedpans,
blood-pressure cuffs, and bedrails.
• In between those two scenarios, a “semi-critical” level of disinfection
presents a medium risk of contamination. This would include equipment
such as endoscopes used on mucous membranes or areas of broken skin.
7. Spaulding divided the three levels of criticality into four classifications for disinfection or
sterilization requirements:
• Sterilization – The highest level of decontamination requires that all
microbes have been destroyed.
• High-level disinfection – HLD is considered semi-critical and requires the
destruction of all bacteria, fungi, mycobacteria, and viruses, though small
numbers of bacterial spores may remain.
• Intermediate-level disinfection – The ILD designation is considered non-
critical, but requires the destruction of most bacteria, mycobacteria, and
certain types of viruses, as well as some fungi. It is not required to destroy
any spores.
• Low-level disinfection – LLD is also non-critical. This designation requires
only the destruction of bacteria and enveloped viruses, as well as partially
destroying fungi and non-enveloped viruses. It does not need to destroy
spores or mycobacteria.
11. Critical Items
• Critical items confer a high risk for infection if they are contaminated
with any microorganism.
• Thus, objects that enter sterile tissue or the vascular system must be
sterile because any microbial contamination could transmit disease.
• This category includes surgical instruments, cardiac and urinary
catheters, implants, and ultrasound probes used in sterile body
cavities.
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13. Critical Items
• Most of the items in this category should be purchased as sterile or be sterilized
with steam if possible.
• Heat-sensitive objects can be treated with EtO, hydrogen peroxide gas plasma; or
if other methods are unsuitable, by liquid chemical sterilant.
• Germicides categorized as chemical sterilant include ≥2.4% glutaraldehyde-based
formulations, 0.95% glutaraldehyde with 1.64% phenol/phenate, 7.5% stabilized
hydrogen peroxide, 7.35% hydrogen peroxide with 0.23% peracetic acid, 0.2%
peracetic acid, and 0.08% peracetic acid with 1.0% hydrogen peroxide.
• Liquid chemical sterilant reliably produce sterility only if cleaning precedes
treatment and if proper guidelines are followed regarding concentration, contact
time, temperature, and pH.
14. Semicritical Items
• Semicritical items contact mucous membranes or nonintact skin. This category
includes respiratory therapy and anesthesia equipment, some endoscopes,
laryngoscope blades , esophageal manometry probes, cystoscopes , anorectal
manometry catheters, and diaphragm fitting rings.
• These medical devices should be free from all microorganisms; however, small
numbers of bacterial spores are permissible. Intact mucous membranes, such as
those of the lungs and the gastrointestinal tract, generally are resistant to
infection by common bacterial spores but susceptible to other organisms, such as
bacteria, mycobacteria, and viruses.
• Semicritical items minimally require high-level disinfection using chemical
disinfectants. Glutaraldehyde, hydrogen peroxide, ortho-phthalaldehyde, and
peracetic acid with hydrogen peroxide are cleared by the Food and Drug
Administration (FDA) and are dependable high-level disinfectants provided the
factors influencing germicidal procedures are met. When a disinfectant is
selected for use with certain patient-care items, the chemical compatibility after
extended use with the items to be disinfected also must be considered.
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16. Semicritical Items
• High-level disinfection traditionally is defined as complete elimination of all microorganisms in or
on an instrument, except for small numbers of bacterial spores. The FDA definition of high-level
disinfection is a sterilant used for a shorter contact time to achieve a 6-log10 kill of an
appropriate Mycobacterium species. Cleaning followed by high-level disinfection should eliminate
enough pathogens to prevent transmission of infection.
• Laparoscopes and arthroscopes entering sterile tissue ideally should be sterilized between
patients.
• As with flexible endoscopes, these devices can be difficult to clean and high-level disinfect or
sterilize because of intricate device design (e.g., long narrow lumens, hinges). Meticulous cleaning
must precede any high-level disinfection or sterilization process.
• Although sterilization is preferred, no reports have been published of outbreaks resulting from
high-level disinfection of these scopes when they are properly cleaned and high-level disinfected.
Newer models of these instruments can withstand steam sterilization that for critical items would
be preferable to high-level disinfection
17. Semicritical Items
• Rinsing endoscopes and flushing channels with sterile water, filtered water, or tap water will prevent adverse
effects associated with disinfectant retained in the endoscope (e.g., disinfectant-induced colitis). Items can
be rinsed and flushed using sterile water after high-level disinfection to prevent contamination with
organisms in tap water, such as nontuberculous mycobacteria, Legionella, or gram-negative bacilli such
as Pseudomonas.
• Alternatively, a tap water or filtered water (0.2m filter) rinse should be followed by an alcohol rinse and
forced air drying.
• Forced-air drying markedly reduces bacterial contamination of stored endoscopes, most likely by removing
the wet environment favorable for bacterial growth. After rinsing, items should be dried and stored (e.g.,
packaged) in a manner that protects them from recontamination.
• Some items that may come in contact with nonintact skin for a brief period of time (i.e., hydrotherapy tanks,
bed side rails) are usually considered noncritical surfaces and are disinfected with intermediate-level
disinfectants (i.e., phenolic, iodophor, alcohol, chlorine).Since hydrotherapy tanks have been associated with
spread of infection, some facilities have chosen to disinfect them with recommended levels of chlorine.
• In the past, high-level disinfection was recommended for mouthpieces and spirometry tubing (e.g.,
glutaraldehyde) but cleaning the interior surfaces of the spirometers was considered unnecessary . This was
based on a study that showed that mouthpieces and spirometry tubing become contaminated with
microorganisms but there was no bacterial contamination of the surfaces inside the spirometers. Filters have
been used to prevent contamination of this equipment distal to the filter; such filters and the proximal
mouthpiece are changed between patients.
18. Noncritical Items
• Noncritical items are those that come in contact with intact skin but not
mucous membranes. Intact skin acts as an effective barrier to most
microorganisms; therefore, the sterility of items coming in contact with
intact skin is “not critical.”
• In this guideline, noncritical items are divided into noncritical patient care
items and noncritical environmental surfaces.
• Examples of noncritical patient-care items are bedpans, blood pressure
cuffs, crutches and computers . In contrast to critical and some semi-critical
items, most noncritical reusable items may be decontaminated where they
are used and do not need to be transported to a central processing area.
Virtually no risk has been documented for transmission of infectious agents
to patients through noncritical items when they are used as noncritical
items and do not contact non-intact skin and/or mucous membranes.
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20. • Non-critcal environmental surfaces include bed rails, some food utensils, bedside
tables, patient furniture and floors. Noncritical environmental surfaces frequently
touched by hand (e.g., bedside tables, bed rails) potentially could contribute to
secondary transmission by contaminating hands of health-care workers or by
contacting medical equipment that subsequently contacts patients.13, 46-48, 51, 66,
67 Mops and reusable cleaning cloths are regularly used to achieve low-level
disinfection on environmental surfaces. However, they often are not adequately
cleaned and disinfected, and if the water-disinfectant mixture is not changed
regularly (e.g., after every three to four rooms, at no longer than 60-minute
intervals), the mopping procedure actually can spread heavy microbial
contamination throughout the health-care facility.68 In one study, standard
laundering provided acceptable decontamination of heavily contaminated
mopheads but chemical disinfection with a phenolic was less
effective.68 Frequent laundering of mops (e.g., daily), therefore, is recommended.
Single-use disposable towels impregnated with a disinfectant also can be used for
low-level disinfection when spot-cleaning of noncritical surfaces is needed