Southern blotting is a technique discovered in 1975 by Edwin Mellor Southern used to analyze DNA. It involves separating DNA fragments via gel electrophoresis, transferring them to a nitrocellulose membrane, then using a probe to hybridize and visualize specific DNA sequences through autoradiography. The key steps are extracting DNA from cells, restriction enzyme digestion, gel electrophoresis, blotting fragments to a membrane, and hybridizing a probe for detection and analysis of target DNA sequences.