This is a new product of Actoprobe LLC: Scanning Laser Confocal Microscope. I designed and built the microscope to investigate the defects and laser burn patterns on the mirror and LBO crystal surfaces. The microscope can also be used for bio-imaging.

1. Actoprobe Scanning Laser Confocal Microscope
DesignedandBuiltby
Dr.PranavRathi

2. Laser Damage (IPG Photonics)
Mirror LBO
LBO crystal surface

3. SLM Overview
 Designed using top class off-the-
shelf components
 Fully automated
 Fast Imaging capabilities:
2 fp/sec to 30 fp/sec
 Optical sectioning available up to
100 µm
 Field of scan up to 280 µms
 Suitable for wavelengths from
300 to 700 nm
 Compatible with most AFMs
 Compatible with upright and
inverted microscope configurations

4. Dimensions
33 inches
20 inches16 inches
Enclosure

5. Enclosure
System offers very
durable and sturdy
enclosure to protect
the microscope from
airborne noise and
dust

6. Components
Scanner and Confocal optics:
Galvano Scanners and optical
fiber optics
Color Camera
Objectives: Can hold
up to 5 objectives
Sample Holder Stage
Lasers and
fiber launcher:
Accommodate
up to 2 lasers
Detector: Compatible
with PMT, APD or
Silicon detectors
White light source

7. Confocal System
 1kHz or 8kHz Scanners
 2 fp/sec to 30fp/sec acquisition
rate at 512X512 pixel resolution
 Confocal aperture diameters up to
1.8 µm
 Fiber coupled: Suitable for
wavelengths from 300 to 700 nm
 Suitable for objectives from 10X to
100X
 Suitable for PMT, APD or Silicon
detectors
 Field of View up to 280X280 µm
 Lateral resolution up to 270 nm and
axial resolution up to 370 nm

8. Sample Holder Stage
 Crystal Holder (New Port)
 Specially Designed Crystal
Holder adapter
 Tip-Tilt-Rotation stage: 5
degrees tip, tilt and rotation
 Z Piezo Stage: 100 µm travel
range close loop with .2 nm
resolution for optical
sectioning
 X-Y Stage: 25 mm travel
range close loop with 7 nm
resolution
 Focus Block: 2 inches travel
range in Z with 1 µm accuracy
 Automated stage (X-Y-Z)
controlled through JoyStick
NLO Crystal

9. Laser - Fiber Launcher
 ND filter: Each laser has an
independent intensity control
 Lasers: 2 to 3 lasers can be used
(RGB); current lasers are 100 mW
blue and green
 Signal optimization through
chopper
 Fiber launcher: 2 to 3 fibers can
be launched; current
configuration- 2 SM fibers (blue
and green)

10. Software: Data Acquisition GUI

11. Sample Holder Stage GUI

12. Camera GUI

13. cData Analysis GUI

14. Quick Data Plotter GUI

15. Optical sectioning of a laser burn on mirror surface
Camera Image
3D Image constructed from individual slices
Slice at the top surface
Slice at 1.5 µm depthSlice at 1 µm depth
Slice at 2.5 µm depthSlice at 2 µm depth
Slice at 500nm depth

16. Optical sectioning of a laser burn on LBO crystal surface
Camera Image
3D Image constructed from individual slices
Slice at the top surface of pattern Slice at 4 µm depth
Slice at 2 µm depth Slice at 6 µm depth

17. Optical sectioning of a laser burn on LBO crystal surface
Camera Image
3D Image constructed from individual slices
Slice at the top surface of pattern Slice at 600 nm depth
Slice at 400 nm depth Slice at 800 nm depth

18. Confocal Microscope with Free Space Alignment Arm
By:
Dr. Pranav Rathi