Scanning probe microscopy (SPM) uses a probe that interacts with the sample surface without lenses to resolve images. The first SPM was invented in 1981 by Binning and Roher, winning them the Nobel Prize. For SPM techniques like STM and AFM to provide atomic-level surface structure information, the tip-sample position must be controlled within 0.1 Angstroms and the tip must be very sharp. STM uses tunneling current between a biased tip and conducting sample, while AFM measures cantilever deflection from tip-surface interactions to map topography. SPM provides higher resolution than diffraction-limited techniques and can image insulators and conductors.
Scanning Probe microscopy (AFM and STM) head point
AFM: Configuration of AFM
Parts of AFM system and Principle of AFM
Three Modes of AFM
AFM Instrument
Advantage and disadvantage
STM
Schematic Diagram
AFM and STM
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A scanning electron microscope is a type of electron microscope that produces images of a sample by scanning the surface with a focused beam of electrons. The electrons interact with atoms in the sample, producing various signals that contain information about the sample's surface topography and composition.
SEMs can magnify an object from about 10 times up to 300,000 times. A scale bar is often provided on an SEM image. From this the actual size of structures in the image can be calculated.
Scanning Probe microscopy (AFM and STM) head point
AFM: Configuration of AFM
Parts of AFM system and Principle of AFM
Three Modes of AFM
AFM Instrument
Advantage and disadvantage
STM
Schematic Diagram
AFM and STM
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Please like, share, comment and follow.
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If any query then contact:
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Thanking-You
Preeti Choudhary
A scanning electron microscope is a type of electron microscope that produces images of a sample by scanning the surface with a focused beam of electrons. The electrons interact with atoms in the sample, producing various signals that contain information about the sample's surface topography and composition.
SEMs can magnify an object from about 10 times up to 300,000 times. A scale bar is often provided on an SEM image. From this the actual size of structures in the image can be calculated.
Transmission electron microscopy (TEM)- by sivasangari Shanmugam. Transmission electron microscopy (TEM) is a technique used to observe the features of very small specimens.
SEM is a type of electron microscope designed for directly studying the surfaces of solid objects, that utilizes a beam of focused electron of relatively low energy as an electron probe that is scanned in a regular manner over the specimen.
Surface Plasmon Resonance,
Surface Plasmons:
Plasmons confined to surface (interface) and interact with light resulting in polarities.
Propagating electron density waves occurring at the interface between metal and dielectric.
A scanning electron microscope (SEM) is a type of electron microscope that produces images of a sample by scanning the surface with a focused beam of electrons.
Transmission Electron Microscope (TEM), RESOLVING POWER, Scanning Electron Microscope, PRINCIPLE AND WORKING OF SEM, SEM SAMPLE PREPARATION, Limitations of Scanning Electron Microscopy (SEM), ADVANTAGES & DISADVANTAGES OF SEM, APPLICATIONS OF SEM, PRINCIPLE, AND WORKING OF TEM, SAMPLE PREPARATION FOR TEM, ADVANTAGES & DISADVANTAGES OF TEM, APPLICATIONS OF TEM, Differences between SEM and TEM.
electron scattering,SEM,TEM,tunnel effect and lensesKASHISHMANGAL2
it can give you the brief view about the effects and lense used for electron microscope i.e. SEM,TEM,electron scattering,tunnel effect,electrostatic lens and magnostatic lens
Transmission electron microscopy (TEM)- by sivasangari Shanmugam. Transmission electron microscopy (TEM) is a technique used to observe the features of very small specimens.
SEM is a type of electron microscope designed for directly studying the surfaces of solid objects, that utilizes a beam of focused electron of relatively low energy as an electron probe that is scanned in a regular manner over the specimen.
Surface Plasmon Resonance,
Surface Plasmons:
Plasmons confined to surface (interface) and interact with light resulting in polarities.
Propagating electron density waves occurring at the interface between metal and dielectric.
A scanning electron microscope (SEM) is a type of electron microscope that produces images of a sample by scanning the surface with a focused beam of electrons.
Transmission Electron Microscope (TEM), RESOLVING POWER, Scanning Electron Microscope, PRINCIPLE AND WORKING OF SEM, SEM SAMPLE PREPARATION, Limitations of Scanning Electron Microscopy (SEM), ADVANTAGES & DISADVANTAGES OF SEM, APPLICATIONS OF SEM, PRINCIPLE, AND WORKING OF TEM, SAMPLE PREPARATION FOR TEM, ADVANTAGES & DISADVANTAGES OF TEM, APPLICATIONS OF TEM, Differences between SEM and TEM.
electron scattering,SEM,TEM,tunnel effect and lensesKASHISHMANGAL2
it can give you the brief view about the effects and lense used for electron microscope i.e. SEM,TEM,electron scattering,tunnel effect,electrostatic lens and magnostatic lens
Electron beam lithography (often abbreviated as e-beam lithography or EBL) is the process of transferring a pattern onto the surface of a substrate by first scanning a thin layer of organic film (called resist) on the surface by a tightly focused and precisely controlled electron beam (exposure) and then selectively removing the exposed or nonexposed regions of the resist in a solvent (developing). The process allows patterning of very small features, often with the dimensions of submicrometer down to a few nanometers, either covering the selected areas of the surface by the resist or exposing otherwise resist-covered areas. The exposed areas could be further processed for etching or thin-film deposition while the covered parts are protected during these processes. The advantage of e-beam lithography stems from the shorter wavelength of accelerated electrons compared to the wavelength of ultraviolet (UV) light used in photolithography.
In EBL, a resist layer is directly patterned by scanning with an electron beam electronically. Modern EBL systems have very good depth of focus (several hundred nanometres) and are able to correct for large-scale height variations of the wafer (of several hundred microns), and so are able to cope well with the rough surface topology of typical GaN wafers and associated wafer bow. EBL also has the advantage of allowing multiple designs to be fabricated together on one wafer. EBL is, however, a slow and expensive process, which is not practical for production. Substrate charging and proximity error effects must be taken into account to get good quality devices. Charging effects can be overcome by application of a sub-nanoscale removable conductive layer on top of the resist. Proximity error correction effects are overcome using specialised design correction software.
Electron microscope, principle and applicationKAUSHAL SAHU
Introduction
History
Resolution &Magnification of
Electron microscope
Types of electron microscope
1) Transmission electron microscope (TEM)
- Structural parts of TEM
- Principle & Working of TEM
- Sample preparation for TEM
- Advantages & disadvantages of TEM
Scanning electron microscope (SEM)
- Structural parts of SEM
- Principle & Working of SEM
- Sample preparation for SEM
- Advantages & disadvantages of SEM
3) Scanning transmission electron microscope (STEM)
Applications of electron microscope
Conclusion
References
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
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Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
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Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
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Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
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(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
1. THE INSTITUE OF SCIENCE .
TOPIC :Scanning probe
microscopy.
-sana shaikh
m.sc(physical chemistry)
Paper-2.
2. Scanning probe microscopy (SPM),
• First scanning probe microscope invented in 1981
by Binning and Roher ,for which they received the
Nobel prize.
• The family of SPM uses no lenses ,but rather a
probe that interacts with the sample surface.
• Simple design.
• Low cost .
• Easy to handle
• Automatically resolves images
6. SPM.
For the techniques to provide
information on the surface
structure at the atomic level:
• the position of the tip with
respect to the surface must
be very accurately controlled
(to within about 0.1 Å) by
moving either the surface or
the tip.
It is possible to accurately
control the relative positions
of tip and surface by ensuring
good vibrational isolation of
the microscope and using
sensitive piezoelectric
positioning devices.
• the tip must be very sharp -
ideally terminating in just a
single atom at its closest point
of approach to the surface.
7.
8. STM.
• It has metal needle that scans sample by
moving back and forth over it, gathering
information about the curvature of the
surface.
• STM uses sharp conducting tip with bias
voltage applied between the tip and the
sample.
• When the tip is brought within about 10A of
the sample ,electrons from the sample being
to “tunnel” through the 10 A gap into the tip
or vice versa depending upon the sign of the
bias voltage.
• The resulting current varies with the tip-to-
sample spacing and it is the signal used to
create an STM image.
• STM is designed to scan a sample in either of
two modes
• Constant –height mode
• Constant –current mode.
9.
10. AFM.
• The AFM probes the surface of
sample with a sharp tip, a couple
of microns long often less than
100A in diameter .the tip is
located at the free end of the
cantilever that is 100 to 200 Um
long.
• Force between tip and the
sample surface causes the
cantilever to bend or deflect.
• A detector measures the
cantilever deflection allow a
computer to generate a map of
surface topography .
• Can be used to study insulators
and semi conductors as well as
electrical conductors.
11.
12. Advantages.
• The resolution of the microscopes is not limited by
diffraction, only by the size of the probe-sample
interaction volume .
• Hence the ability to measure small local differences in
object height (like that of 135 picometres steps on <100>
silicon) is unparalleled. Laterally the probe-sample
interaction extends only across the tip atom or atoms
involved in the interaction.
• The interaction can be used to modify the sample to create
small structures (Scanning probe lithography).
• Unlike electron microscope methods, specimens do not
require a partial vacuum but can be observed in air at
standard temperature and pressure or while submerged in
a liquid reaction vessel.
13. Disadvantages
• The detailed shape of the scanning tip is sometimes
difficult to determine. Its effect on the resulting data is
particularly noticeable if the specimen varies greatly
in height over lateral distances of 10 nm or less.
• The scanning techniques are generally slower in
acquiring images, due to the scanning process. As a
result, efforts are being made to greatly improve the
scanning rate.
• The maximum image size is generally smaller.
• Scanning probe microscopy is often not useful for
examining buried solid-solid or liquid-liquid interfaces.
14. Reference:-
• Skoog D A, West D M , Fundamentals of
Analytical Chemistry, Thomson
• Asia Pvt ltd., 8 th Ed, (2004)
• 2) Skoog, Holler, Nieman, Principles of
Instrumental Analysis
• http://teachers.stanford.edu/activities/SPMRe
ference/SPMReference.pdf
• http://slideplayer.com/slide/4959102/