Optical tweezers are affected by airborne and mechanical noises; this presentation is a saga of my struggle with it.

1. Search for the noise-source and remedy for Optical tweezers http://openwetware.org/wiki/User:Pranav_Rathi/Notebook/OT/2011/06/21/Noise_issue_with_the_optical_tweezers

2. Steve-office power spectrum Graph 1 120Hz major frequency Graph 2 120Hz major frequency Graph 3 120Hz major frequency

3. Steve-DAQ2 power spectrum Graph 1 120Hz major frequency 210Hz major frequency 120Hz major frequency Graph 1 Graph 1

4. X-Piezo and Z-Piezo power spectrum Graph 1 Graph 2 180Hz major frequency 130Hz & 2155Hz major frequency

5. Luca and cooling fans power spectrum Graph 1 Graph 2

6. Airborne noise spectrum in the lab Midlab1 Midlab2 Near hood wall 150 and 270 Hz major frequency 120 Hz major frequency 150 Hz major frequency

7. Near exhaust Inside hood 150 Hz major frequency 150 Hz major frequency

8. Chassis/lab wall power spectrum Ch/lab wall1 Ch/lab wall2 Hoodwall1 150 Hz major frequency Broad disperse band of frequencies 150 Hz major frequency

9. Airborne noise spectrum in the Chassis AC unit 150 Hz major frequency Graph 1 Graph 2 Graph 3 270 Hz major frequency 125 Hz major frequency

10. Steve office Steve DAQ2 X-Piezo Z-Piezo Luca camera Hood 150, 270, 225 Hz 150, 125, 270, 435, 535 Hz Chassis 120 Hz ≤ -10.8 225,945,1170 Hz 455,210,140Hz 180 Hz ≤ -9 260,390,520,650…Hz 130 Hz ≤ -2.4 Midlab 120 Hz Nearhood 150 Hz Midlab 150 ,270Hz Wall 165,220,270Hz Trap Air borne & vibration Air borne vibration Air borne Air borne & vibration Active Passive Summary

11. Remedy: Design and Solution

12. Old design: Trap-setup for sample holder stages Sample holder stage X- Piezo stage Piezo holder stage Vibrations Vibrations

13. New design: Trap-setup for sample holder stages Sample holder stage X- Piezo stage Piezo holder stage

14. Sample holder stage (plate) Aluminum stage Acrylic plate regular Acrylic plate advance

15. Sketch-up for sample holder plates

16. Test setup sensor source Diaphragm cutoff vibrations

17. Test result of black aluminum platform

18. Test setup for aluminum plate vibrations

19. Test setup for plastic plate vibrations

20. Result comparison of sample holder plates

21. Piezo holder stages Aluminum stage Plastic stage regular Plastic stage advance upper bottom

22. Sketch-up for Piezo holder stages

24. Test setup sensor source Diaphragm cutoff vibrations

25. Test setup for aluminum stage vibrations

26. Test setup for plastic stages Acoustic foam curtain

27. Result comparison of piezo holder stages

28. Microscope breadboard platform (isolator)

29. Top View Screw hold Plate hold

30. Bottom View Regular feet Holy feet 3”

31. Microscope breadboard platform test setup Speaker Sensor vibrations

32. Speaker Sensor Microscope breadboard platform test setup Screw hold, Regular feet

33. Microscope breadboard platform test setup Plate hold, Holy feet

34. Microscope breadboard platform Result

36. Dichroic holder stage Regular stage Lens holder

37. Regular dichroic stage test setup

39. Designed dichroic holder stage platform

40. Holder Dichroic holder stage

41. From below Front view Dichroic holder stage holder

43. Lens holder 2d holder Lens tube clamps

44. Lens tube 2d-holder clams

46. Advance-design dichroic stage

50. Advance-design dichroic stage test setup

51. Optical table Vs breadboard on regular stage

52. Optical table Vs breadboard on Advance stage

53. Regular Vs advance dichroic stage on optical table test Result

54. Regular Vs advance dichroic stage on breadboard test Result

55. Comparison among regular stage on OT&BB and adv. Stage on OT&BB