Nano-Spectrophotometer
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Nano spectrophotometer with full spectrum light covers wide wavelength range of 200 to 800nm. It requires only 0.5 - 2.0 µl micro-volume sample for measurement which takes place within 5 seconds. It utilizes CCD detector, Xenon flash lamp as light source which has high stability and long operating life. They are used to detect micro volume quantities of nucleic acids and other concentrated or dilute components.
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We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
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spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
What is greenhouse gasses and how many gasses are there to affect the Earth.
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
RNA INTERFERENCE: UNRAVELING GENETIC SILENCING
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
SCHIZOPHRENIA Disorder/ Brain Disorder.pdf
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
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Multi-source connectivity as the driver of solar wind variability in the heli...
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
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holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
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Orion Air Quality Monitoring Systems - CWS
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
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Nano-Spectrophotometer
- 1. Nano Spectrophotometer LNS-A10 www.labtron.uk | info@labtron.uk
- 2. Overview Nano spectrophotometer with full spectrum light covers wide wavelength range of 200 to 800nm. It requires only 0.5 - 2.0 µl micro-volume sample for measurement which takes place within 5 seconds. It utilizes CCD detector, Xenon flash lamp as light source which has high stability and long operating life. They are used to detect micro volume quantities of nucleic acids and other concentrated or dilute components. Specifications : Wavelength range 200 - 800 nm Path length 0.2 mm (For high concentration measurement); 1.0 mm (For ordinary) Wavelength accuracy ± 1 nm Wavelength Resolution ≤ 3 nm (FWHM at Hg 546 nm) Minimum Sample size 0.5 - 2.0 µl Absorbance range 0.02 - 90 A (10mm equivalent) Absorbance precision 0.003 Abs Absorbance accuracy 1 % ( 7.332 Abs at 260 nm) Light source Xenon flash lamp Measurement time < 8 s Operating Voltage 24 V DC Sample Pedestal Material Aluminum alloy and Quartz fiber Detector Type 3864-element linear silicon CCD array Software Compatibility Windows 7, Windows XP Detects nucleic acid up to 2 - 4500 ng/µL (dsDNA)
- 3. Operating Power Consumption 20 W Standby Power Consumption 5 W Dimension 200 x 250 x 166 mm Weight 2.6 kg Features : - 0.5 µl - 2.0 µl micro-volume sample for direct measurement onto pedestal - Wavelength range 200 - 800 nm with scan capability within 5 seconds - User friendly software - Long life Xenon flash lamp (10 flashes up to 10 years) - Highly concentrated samples can be used - Direct Concentration display - No cell or cuvettes are required Applications : Nano spectrophotometer are used in detection of micro volume quantities of DNA/RNA and chemicals , liquids and its components in laboratory, food industries, chemistry, microbiology, medicine research and development.
- 4. Labtron Equipment Ltd.Quatro House,Lyon Way,Camberley,SurreyGU16 7ER, United Kingdom Email: | Phone: +44 2080 043 608 info@labtron.uk Website: www.labtron.uk