This document discusses the topic of nanobioremediation. It begins with introductions to nanoscience and bioremediation. Nanobioremediation uses nanoscience and nanoparticles to enhance microbial activity and remove pollutants more quickly. Examples are given of using genetic engineering and nanoparticles to modify microbes for improved bioremediation. The document concludes that nanobioremediation can clean environmental hazards faster and safer than other methods while meeting economic, social, and environmental criteria.

1. NANO BIOREMEDIATION
Presented by-
Shreya M. Modi,
M.Phil/P.hD Student
in Nanosience

2. Content
 Introduction
 Bioremediation
 Nanobioremediation
 Applications
 Conclusion
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3. INTRODUCTION
• Nanoscience is the study of
atoms, Molecules, and objects whose size is on
nanometer scale.
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4. What Is Nanotechnology?
Ability to understand, create, and use
structures, devices and systems that have
fundamentally new properties and
functions because of their Nano scale
structure
Ability to image, measure, model, and Nano area Electron Diffraction
manipulate matter on the Nano scale to of DW Carbon Nanotube –
Zuo, et.al
exploit those properties and functions
Ability to integrate those properties and
functions into systems spanning from
nano- to macro-scopic scales
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Corral of Fe Atoms – D. Eigler

5. Components
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6. 6

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8. BIOREMEDIATION
• The degradation of environmental waste using
microorganisms is known as BIOREMEDIATION.
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9. Biodegradable Contaminants
Micro
organisms
Convert them to
Grow on toxic
nontoxic
compounds products
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10. Classes of Contaminants
Classes of
Contaminants
Chlorinated Toxic
Aromatics solvents
Oxygenases
metals
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11. NANOBIOREMEDIATION
• The use of Nanoscience, Nanoparticals and
Nanotechnology to enhance the microbial activity
to remove pollutants.
Coast
NBR
Clean up
Contaminants
time
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12. GENETIC ENGINEERING OF
MICROBES
• Nanotechnology and its tools play very important
role in genetic engineering of microbes.
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13. Wide
Physiological
Diversity
Controlled Micro Small
Culturability organisms Size
Genetic
Manipulability
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14. http://www.sciencedirect.com/science/article/pii/S0378111911000916 14

15. Deinococcus radiodurans
World’s Strongest Bacteria
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16. Mer A LOCUS of E.coli.
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17. Conti………
• Deinococcus radiodurans, which naturally reduces
Cr(VI) to the less mobile and less toxic Cr(III), was
engineered for complete toluene degradation by cloned
expression of tod and xyl genes of Pseudomonas putida.
http://ars.els-cdn.com/content/image/1-s2.0-S0168165600003679-gr3.gif 17

18. NANOSCALE BIOPOLYMERS
Nanoscale modified biopolymers which can be
manufactured by genetic and protein engineering of
microorganisms which can control the size and
arrangement at the molecular level.
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19. DECOLORIZATION OF THE DYE CONGORED
BY Aspergillus niger SILVER NANOPARTICALS
SEM image of the Aspergillus niger AgNP
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20. % of decolorizationof the dye Congo red by
Aspergillusniger silver nanoparticle and Aspergillus
niger (plain culture)
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21. REMOVAL OF PHENOLIC POLLUTANTS FROM
MUNICIPAL WASTE WATER BY IMMOBILIZED
LACCASE ENZYMES USING NANOPARTICALS
Endocrine disrupting compounds
In the present work laccase of a Thielavia genus has been
immobilized on fumed silica nanoparticles.
Mesoporous carbon materials, have been considered as highly
suitable candidates for laccase enzyme isolated from Trametes
versicolor and molecule immobilization . 21

22. Degradation of Hydrophobic Compound
Enhanced by Nanoparticals
• Polymeric nanoparticals prepared from a
poly(ethylene) glycol Modified Urethane
Acrylite(PMUA) precursor was applied to enhance
the bioavailability of Polynuclear Aeromatic
Hydrocarbons(PAHs) in soil and aqueus solutions.
• Due to the hydrophobicity of interior regions of
PMUA there is increased affinity between PAHs and
released into the aqueous phase and enhances the rate
of Mineralization.
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23. NANOTECH COATING CAN ENHANCE
ELECTRICITY OUTPUT FROM
WASTE WATER
• In this technology, bacteria from biowaste
such as sewage are placed in an anode
chamber, where they form a biofilm,
consume nutrients and grow, in the process
releasing electrons. In this context, the
sewage is literally the fuel for electricity
production.
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24. They found that coating graphite anodes with a
nanoparticle layer of gold can increase electricity
production by 20 times, while coatings with palladium
produced an increase as well, but not nearly as much
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25. • Silver nanoparticles (Ag-NPs) in particular,
provide effective growth inhibition of various
microorganisms in suspension and on solid
medium.
• In addition, a few types
of filtration membranes
and devices like catheter
incorporating silver nps
have demonstrated
anti-biofouling properties.
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26. NANOBIOREMEDIATION TO CLEAN
UP OIL SPILL
• Immobilization cells of Ps. mendocina H3, Ps.
pseudoalcaligenes H7, Ps. stutzeri H10,
Ps.alcaligenes H15, Ps. pseudoalcaligenes
H16, Ps. mallei 36K and Micrococcus luteus
37 was demonstrated high sorbtional
activity carriers. 26

27. Conti…..
• Bioremediation of oil contaminated soil is
important that CARBONIZATED
SORBENTS itself may sorbs oil drops for
further oxidation carbohydrates of oil by
microbial cells, to be source of mineral
compounds and improve condition of soils.
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28. IMMOBILIZATION OF MICROBIAL
CELLS USING NANOPARTICALS
• Magnetic nanoparticals (Fe3O4) were
functionalized with ammonium oleate and coated
on the surface of Pseudomonas delafieldii.
• On application of external magnetic field to the
microbial cells, the nanopartical coated cells
concentrate on particular site of the reactor wall
separating them from the whole solution and
enabling recycling of the cells for the treatment of
the same compound.
http://www.ncbi.nlm.nih.gov/pmc/articles
/PMC1183266/figure/f2/ 28

29. Bio desulfurization (BDS) of dibenzothiophene
(DBT) was carried out by Rhodococcus erythropolis
IGST8 decorated with magnetic Fe3O4
nanoparticles, synthesized in-house by a chemical
method, with an average size of 45–50 nm
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30. 30

31. MICROBIAL PRODUCTION OF SELENIUM
NANOPARTICLES USED FOR WASTE WATER
TREATMENT AND OHER APPLICATION
http://www.sciencedirect.com/science/article/pii/S
0304389411001014
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32. CONCLUSION
• According to above all application of
Nanobioremediation it can be definitely
concluded that, Nanoparticals,
Nanotechnological instrument play efficient
role in the process of Nanobioremediation. By
applying the nanobioremediation to
environment hazards, it can clean them Faster
and Safer than other methods and technology.
We can say that, Nanobioremediation Maintain
all three criteria.
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33. Social
NMR
Economical Biological
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34. Thank You
• Thank you.
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