Protein engineering is the modification of proteins using recombinant DNA technology or chemical treatment to achieve a desired function. It involves disciplines like molecular biology, protein chemistry, and structural biology. The objectives are to create superior enzymes for industrial use, produce biological compounds in large quantities, and develop more potent pharmaceuticals. Key methods are genetic modification techniques like mutagenesis and gene cloning to alter stability and activity, as well as chemical modifications like PEGylation to increase enzyme half-life. Significant progress has been made in engineering proteins like insulin, interferon, and antibodies for improved properties.
Expression and purification of recombinant proteins in Bacterial and yeast sy...Shreya Feliz
This presentation gives the information about bacterial and yeast system as host for expressing recombinant proteins, suitable vectors, strains of host, Pros and cons of this system, different purification techniques and commercially available proteins produced so far by this system.
Being able to identify genes, compare them, analyze them could be applied in various research areas from medical to industrial.
This ppt is designed for Health science and computational biology students to enable you understand the above mentioned topic.
Expression and purification of recombinant proteins in Bacterial and yeast sy...Shreya Feliz
This presentation gives the information about bacterial and yeast system as host for expressing recombinant proteins, suitable vectors, strains of host, Pros and cons of this system, different purification techniques and commercially available proteins produced so far by this system.
Being able to identify genes, compare them, analyze them could be applied in various research areas from medical to industrial.
This ppt is designed for Health science and computational biology students to enable you understand the above mentioned topic.
It is a presentation showing the process of doing a prokaryotic genome annotation using RAST server. It is a basic work in bioinformatics field. Genome annotation is total genome analysis of an organism. We can easily do it using bioinformatics tool like RAST server.
This presentation gives an easy introduction to ChIP-seq analyses and is part of a bioinformatics workshop. The accompanying websites are available at http://sschmeier.github.io/bioinf-workshop/#!galaxy-chipseq/
Proteomics studies play an increasing role in the field of biology. The use of mass spectrometry (MS) in combination with a range of separation methods is the main principal methodology for proteomics. The two principal approaches to identifying and characterizing proteins using MS are the “bottom-up”, which analyze peptides by proteolytic digestion, and “top-down”, which analyze intact proteins.
It is a presentation showing the process of doing a prokaryotic genome annotation using RAST server. It is a basic work in bioinformatics field. Genome annotation is total genome analysis of an organism. We can easily do it using bioinformatics tool like RAST server.
This presentation gives an easy introduction to ChIP-seq analyses and is part of a bioinformatics workshop. The accompanying websites are available at http://sschmeier.github.io/bioinf-workshop/#!galaxy-chipseq/
Proteomics studies play an increasing role in the field of biology. The use of mass spectrometry (MS) in combination with a range of separation methods is the main principal methodology for proteomics. The two principal approaches to identifying and characterizing proteins using MS are the “bottom-up”, which analyze peptides by proteolytic digestion, and “top-down”, which analyze intact proteins.
Meta-genomics is the application of modern genomics techniques to the study of communities of microbial organisms directly in their natural environments, bypassing the need for isolation and lab cultivation of individual species”
Protein engineering is the process of developing useful or valuable proteins.
Protein Engineering is a second generation of recombinant DNA technology.
It involves altering cloned DNA in vitro by novel mutational technique so that translated proteins have slightly altered properties.
Introduction
Classification
Therapeutic values of peptidomimetics
Design of peptidomimetics by manipulation of amino acids
Modification of peptide backbone
Chemistry of prostaglandins, leukotrienes and thromboxanes
GraphRAG is All You need? LLM & Knowledge GraphGuy Korland
Guy Korland, CEO and Co-founder of FalkorDB, will review two articles on the integration of language models with knowledge graphs.
1. Unifying Large Language Models and Knowledge Graphs: A Roadmap.
https://arxiv.org/abs/2306.08302
2. Microsoft Research's GraphRAG paper and a review paper on various uses of knowledge graphs:
https://www.microsoft.com/en-us/research/blog/graphrag-unlocking-llm-discovery-on-narrative-private-data/
Slack (or Teams) Automation for Bonterra Impact Management (fka Social Soluti...Jeffrey Haguewood
Sidekick Solutions uses Bonterra Impact Management (fka Social Solutions Apricot) and automation solutions to integrate data for business workflows.
We believe integration and automation are essential to user experience and the promise of efficient work through technology. Automation is the critical ingredient to realizing that full vision. We develop integration products and services for Bonterra Case Management software to support the deployment of automations for a variety of use cases.
This video focuses on the notifications, alerts, and approval requests using Slack for Bonterra Impact Management. The solutions covered in this webinar can also be deployed for Microsoft Teams.
Interested in deploying notification automations for Bonterra Impact Management? Contact us at sales@sidekicksolutionsllc.com to discuss next steps.
State of ICS and IoT Cyber Threat Landscape Report 2024 previewPrayukth K V
The IoT and OT threat landscape report has been prepared by the Threat Research Team at Sectrio using data from Sectrio, cyber threat intelligence farming facilities spread across over 85 cities around the world. In addition, Sectrio also runs AI-based advanced threat and payload engagement facilities that serve as sinks to attract and engage sophisticated threat actors, and newer malware including new variants and latent threats that are at an earlier stage of development.
The latest edition of the OT/ICS and IoT security Threat Landscape Report 2024 also covers:
State of global ICS asset and network exposure
Sectoral targets and attacks as well as the cost of ransom
Global APT activity, AI usage, actor and tactic profiles, and implications
Rise in volumes of AI-powered cyberattacks
Major cyber events in 2024
Malware and malicious payload trends
Cyberattack types and targets
Vulnerability exploit attempts on CVEs
Attacks on counties – USA
Expansion of bot farms – how, where, and why
In-depth analysis of the cyber threat landscape across North America, South America, Europe, APAC, and the Middle East
Why are attacks on smart factories rising?
Cyber risk predictions
Axis of attacks – Europe
Systemic attacks in the Middle East
Download the full report from here:
https://sectrio.com/resources/ot-threat-landscape-reports/sectrio-releases-ot-ics-and-iot-security-threat-landscape-report-2024/
Builder.ai Founder Sachin Dev Duggal's Strategic Approach to Create an Innova...Ramesh Iyer
In today's fast-changing business world, Companies that adapt and embrace new ideas often need help to keep up with the competition. However, fostering a culture of innovation takes much work. It takes vision, leadership and willingness to take risks in the right proportion. Sachin Dev Duggal, co-founder of Builder.ai, has perfected the art of this balance, creating a company culture where creativity and growth are nurtured at each stage.
Transcript: Selling digital books in 2024: Insights from industry leaders - T...BookNet Canada
The publishing industry has been selling digital audiobooks and ebooks for over a decade and has found its groove. What’s changed? What has stayed the same? Where do we go from here? Join a group of leading sales peers from across the industry for a conversation about the lessons learned since the popularization of digital books, best practices, digital book supply chain management, and more.
Link to video recording: https://bnctechforum.ca/sessions/selling-digital-books-in-2024-insights-from-industry-leaders/
Presented by BookNet Canada on May 28, 2024, with support from the Department of Canadian Heritage.
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
UiPath Test Automation using UiPath Test Suite series, part 3DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 3. In this session, we will cover desktop automation along with UI automation.
Topics covered:
UI automation Introduction,
UI automation Sample
Desktop automation flow
Pradeep Chinnala, Senior Consultant Automation Developer @WonderBotz and UiPath MVP
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
"Impact of front-end architecture on development cost", Viktor TurskyiFwdays
I have heard many times that architecture is not important for the front-end. Also, many times I have seen how developers implement features on the front-end just following the standard rules for a framework and think that this is enough to successfully launch the project, and then the project fails. How to prevent this and what approach to choose? I have launched dozens of complex projects and during the talk we will analyze which approaches have worked for me and which have not.
LF Energy Webinar: Electrical Grid Modelling and Simulation Through PowSyBl -...DanBrown980551
Do you want to learn how to model and simulate an electrical network from scratch in under an hour?
Then welcome to this PowSyBl workshop, hosted by Rte, the French Transmission System Operator (TSO)!
During the webinar, you will discover the PowSyBl ecosystem as well as handle and study an electrical network through an interactive Python notebook.
PowSyBl is an open source project hosted by LF Energy, which offers a comprehensive set of features for electrical grid modelling and simulation. Among other advanced features, PowSyBl provides:
- A fully editable and extendable library for grid component modelling;
- Visualization tools to display your network;
- Grid simulation tools, such as power flows, security analyses (with or without remedial actions) and sensitivity analyses;
The framework is mostly written in Java, with a Python binding so that Python developers can access PowSyBl functionalities as well.
What you will learn during the webinar:
- For beginners: discover PowSyBl's functionalities through a quick general presentation and the notebook, without needing any expert coding skills;
- For advanced developers: master the skills to efficiently apply PowSyBl functionalities to your real-world scenarios.
Dev Dives: Train smarter, not harder – active learning and UiPath LLMs for do...UiPathCommunity
💥 Speed, accuracy, and scaling – discover the superpowers of GenAI in action with UiPath Document Understanding and Communications Mining™:
See how to accelerate model training and optimize model performance with active learning
Learn about the latest enhancements to out-of-the-box document processing – with little to no training required
Get an exclusive demo of the new family of UiPath LLMs – GenAI models specialized for processing different types of documents and messages
This is a hands-on session specifically designed for automation developers and AI enthusiasts seeking to enhance their knowledge in leveraging the latest intelligent document processing capabilities offered by UiPath.
Speakers:
👨🏫 Andras Palfi, Senior Product Manager, UiPath
👩🏫 Lenka Dulovicova, Product Program Manager, UiPath
Essentials of Automations: Optimizing FME Workflows with ParametersSafe Software
Are you looking to streamline your workflows and boost your projects’ efficiency? Do you find yourself searching for ways to add flexibility and control over your FME workflows? If so, you’re in the right place.
Join us for an insightful dive into the world of FME parameters, a critical element in optimizing workflow efficiency. This webinar marks the beginning of our three-part “Essentials of Automation” series. This first webinar is designed to equip you with the knowledge and skills to utilize parameters effectively: enhancing the flexibility, maintainability, and user control of your FME projects.
Here’s what you’ll gain:
- Essentials of FME Parameters: Understand the pivotal role of parameters, including Reader/Writer, Transformer, User, and FME Flow categories. Discover how they are the key to unlocking automation and optimization within your workflows.
- Practical Applications in FME Form: Delve into key user parameter types including choice, connections, and file URLs. Allow users to control how a workflow runs, making your workflows more reusable. Learn to import values and deliver the best user experience for your workflows while enhancing accuracy.
- Optimization Strategies in FME Flow: Explore the creation and strategic deployment of parameters in FME Flow, including the use of deployment and geometry parameters, to maximize workflow efficiency.
- Pro Tips for Success: Gain insights on parameterizing connections and leveraging new features like Conditional Visibility for clarity and simplicity.
We’ll wrap up with a glimpse into future webinars, followed by a Q&A session to address your specific questions surrounding this topic.
Don’t miss this opportunity to elevate your FME expertise and drive your projects to new heights of efficiency.
Essentials of Automations: Optimizing FME Workflows with Parameters
Seminar sandy
1. ENGINEERED PROTEIN BY DNA TECHNOLOGY PRESENTED BY: M SANDEEP REDDY. TRINITY COLLEGE OF PHARMACEUTICAL SCIENCES H.NO: 11341P1033 M.Pharmacy (Pharmaceutics) 2 ndsemester
2. DEFINITION Protein engineering can be defined as the modification of protein structure with recombinant DNA technology or chemical treatment to get a desirable function for better use in medicine, industry and agriculture.
3. Protein engineering is merging of several disciplines like: 1.molecular biology.2. protein chemistry.3. enzymology .4.structural chemistry to alter catalytic or structural stability of protein.
4. BASIC ASSUMPTIONS While doing protein engineering should recognize the following properties of , many amino acid substitution, deletions or additions lead to no changes in enzyme activity so that they are silent mutator. Protein have limited number of basic structures and only minor changes are superimposed on them leading to variation Similar patterns of chain folding and domain structure can arise from different amino acid sequences with little or no homology
5. OBJECTIVES OF PROTIEN ENGINEERING The objectives of protein engineering is as follows – (a) to create a superior enzyme to catalyze the production of high value specific chemicals. (b) to produce enzyme in large quantities. (c) to produce biological compounds(include synthetic peptide, storage protein, and synthetic drugs) superior to natural one CONTINUE…
6. Get humanised(chimeric) antibodies with less immunogenicity . Make harmones resistant to attack by antibodies or stomach enzymes. Get more site specific, more potent biopharmaceutical with altered pharmacological action. Over all aim of protien engineering application is to get funtionally more useful enzymes, antibodies, harmones, receptor protiens.
7. Steps involved in protein engineering A study of three dimensional structure of protein A study of three dimensional structure is the preliminary steps of protein engineering. And a 3d structure of protein is produced from the data generated from X-ray crystallography and NMR process by protein modeling
10. You should note that the primary structure has a polarity. That is, there is a N terminal region of the protein and a C terminal region.
11. The curly sections represent alpha helical regions. These are components of the secondary structure of a protein.
12.
13. GENTIC MODIFICATION In the gene modification method is to design, develop and produce proteins with improved operating characteristics ( increased stability & biological activity) sometimes creating even novel proteins. The techniques such as Mutagenesis( site-directed ).& gene cloning are utilized for this purpose.
18. CHANGE OF ASPARAGINE TO OTHER AMINO ACIDS If asparagine and glutamine present in protein when heated, ammonia is released amino acids convert to aspartic acid and glutamic acid. Protein may refold and loss of biological activity. Example: triosephosphateisomerase.
21. SINGLE AMINO ACID CHANGES. Some of the recombinant proteins can be improved in their stability and biology activity by a secondgeneration variants. These have been frequently achieved by a single amino acid changes. Examples: α1 Antitrypsin, insulin, tissue plasminogen activator, hirudin.
22. The other process of gene modification are- In vitro mutagenesis using synthetic oligonucleotides. Synthesis of complete modified gene de novo
23. (a) In vitro mutagenesis using synthetic oligonucleotides. Synthetic oligonucleotides is used for invitro mutagenesis. In this method a small oligonucleotides primer containing the desired modification is first synthesized. It is then hybridized to the appropriate site and cloned gene and then the rest is replicated using DNA polymerase enzyme, so that the rest remains unaltered. This approach is actually used to modify the active site of the tyrosyl-tRNAsynthetase
24. Synthesis of complete modified gene de novo Complete gene in some cases have been chemically synthesized in the form of several oligomers (e.g. genes for insulin, somatostain and interferon), that are ligated in correct order to produce a complete gene. The sequence of the synthetic gene can be designed in a modular fashion to get the desired function.
25. Computer Modeling Gene construction Protein production characterization De novo design of proteins: The attempt to choose an amino acid sequence that is unrelated to any natural sequence, but will fold into a desired 3-D structure with desired properties.
26. Chemical modification of enzymes The protein synthesized under the control of gene sequence in a cell undergo post-transitional modification. This leads to stability, structural integrity, altered solubility and viscosity of individual proteins. for e.g. Enzyme-PEG conjugates. An enzyme L-asparaginase has antitumour properties but is toxic with a life time of less then 18hrs thus reducing its utility. Continue……….
27. L-asparginase can be modified by polyethene glycol derivatives to produce PEG-asparginase conjugates, which differ from the native enzyme in the following way (i) it retains only 52% of the catalytic activity of the native. (ii) it become resistant to proteolytic degradation. (iii) it doesn’t cause allergy.
28. Achievements of protein engineering A number of proteins are known now where efforts have been made to know the effects of site specific mutagenesis involving substitution of one or more amino acids. Insulin- it consist of A and B chains are linked by C-peptide of 35 amino acids. It was shown that a sequence of 6 amino acids for c-peptide was adequate for the linking function. Continue….
29. cytochrome c – A phenylalanine residue has been identified to be non-essential for electron transfer but is involved in determining the reduction potential of the protein. Trypsin- It could be redesigned to have altered substrate specificity.
30. Acetylcholine receptor. This protein is involved in transport, of acetylcholine through. the membrane. Specific regions of this protein involved in acetylcholine binding and channel formation have been, identified. Human beta interferon. Removal of one of the three cysteine residues' I led to an improvement in stability of the enzyme.
31. Conclusion Substantial progress has been made in the of engineered protein by DNA technology . Observations from Genetic modification and Chemical modification , Regarded as redesigning nature rather than copying nature. Protein engineering and genetic engineering are the complementary fields to each other the successes in protein engineering will transform the way we make foods, drugs and chemicals.. Design of protein engineering using DNA technology remains challenging: Though some solutions have been found, few guarantees of success currently exist.
32. References Kuhlman, Brian; Dantas, Gautam; Ireton, Gregory C.; Varani, Gabriele; Stoddard, Barry L. & Baker, David (2003), "Design of a Novel Globular Protein Fold with Atomic-Level Accuracy", Science302 (5649): 1364–1368, 2. Looger, Loren L.; Dwyer, Mary A.; Smith, James J. & Hellinga, Homme W. (2003), "Computational design of receptor and sensor proteins with novel functions", Nature423 (6936): 185–190. 3. www.molecular-plant-biotechnology.info