SEED HARDENING
By,
VENKATESH R
201400400309-10-2018 1
VENKATESH R
B.Sc. AGRICULTURE
ANBIL DHARMALINGAM AGRICULTURAL
COLLEGE AND RESEARCH INSTITUTE
TRICHY-9
09-10-2018 2
09-10-2018 3
INDIA – AN OVERVIEW
09-10-2018 4
LOW PRODUCTIVITY
09-10-2018 5
why?
09-10-2018 6
Poor quality seed
Soil moisture deficit
Low and erratic rainfall
Improper crop management
09-10-2018 7
SEED QUALITY ENHANCEMENT
09-10-2018 8
McDonald (2000) , “Any post harvest treatment
that improves germination or seedling emergence
or facilitate the development of more number of
normal, rapid, uniform and healthy seedlings in
the field conditions.”
09-10-2018 9
09-10-2018 10
SEED HARDENING
09-10-2018 11
Henckel (1964) - “simple method to alter
the physiological and biochemical nature
of the seed in order to induce the factors
responsible for drought resistance”.
09-10-2018 12
It can be done with water / dilute chemical
solutions / growth regulating compounds or
using commonly available natural tonics like
coconut water or milk.
09-10-2018 13
CHEMICALS ROLE
Potassium nitrate Osmo-regulation, cell wall and
membrane permeability
Potassium chloride Activation of enzyme process
Potassium di hydrogen
phosphate
Constituents of phospholipids,
nucleotides and sugar phosphate
Calcium chloride Participate in starch metabolism
09-10-2018 14
09-10-2018 15
BOTANICALS ROLE
Pongam Bio enhancement due to presence of plant
growth hormones
Prosopis Stimulatory effects caused by plant growth
hormones
Moringa Stimulatory effects caused by plant growth
hormones
Pulse sprout extract Induction of sugars and vitamins that
promotes growth
09-10-2018 16
MICROBIAL ENHANCERS ROLE
Rhizobium Induction of root nodulation
Azatobacter Source of PGR
Trichoderma viridi Fungal antagonist
PHYSIOLOGICAL REORGANIZATIONS
Change in hydrophilic property of the
protoplasmic colloids namely the viscosity and
elasticity.
Increase in osmotic potential.
Increase in water holding capacity of plants.
09-10-2018 17
METABOLIC CHANGES
Increased respiration.
Leaves of hardened plants have more starch.
Increased activity of the mitochondria.
Higher rate of photosynthesis because of increase
in the bound water.
09-10-2018 18
MORPHOLOGICAL CHANGES
More xeromorphic morphology.
Epidermal and stomata cells are smaller.
Foliage area is increased.
Faster recovery from atmospheric drought.
More absorbing surface in the root system,
as well as more number of primary roots.
09-10-2018 19
how ?
09-10-2018 20
DRY SEED
SOAKING IN WATER
IMBITION
PERMITS INITIAL GERMINATION PROCESS
DEHYDRATION
PREVENT RADICLE EMERGENCE
SHADE DRY
STOPS GERMINATION PROCESS
HARDENED SEED
SOWING IN DRY LAND
GERMINATION STARTS FROM WHERE IT STOPPED
RAPID ESTABLISHMENT,GROWTH AND YIELD
09-10-2018 21
09-10-2018 22
09-10-2018 23
Hardening techniques for
various crops
09-10-2018 24
Crop Chemical concentration Soaking
duration
(hrs.)
Seed
solution
ratio
Maize,varagu,tenai,samai KH2PO4 2% 8 1:0.6
Pearl millet KCl 2% 10 1:0.6
Sorghum KH2PO4 2% 16 1:0.6
Ragi NaCl 0.2% 6 1:0.7
Cotton KCl 2% 16 1:0.5
Sunflower KCl 2% 12 1:0.6
Red gram ZnSO4 100 ppm 3 1:0.3
Black gram ZnSO4 100 ppm 3 1:0:3
Ground nut CaCl 0.5 % 4 1:0.3
Paddy KCl 1% 16 1:0.6
09-10-2018 25
09-10-2018 26
09-10-2018 27
Effect of seed hardening in Bt. cotton
Treatment Germination Chlorophyll
content
mgg
No of
bollsplant
Yield kgha
CaCl21% 89.7 2.52 31 2518
CaCl2 2% 93.0 3.04 33 2629
CCC
100PPM
91.3 2.62 31 2557
CCC
150PPM
88.7 2.46 29 2492
WATER 84.7 2.15 26 2338
CONTROL 82.7 11.98 25 2276
09-10-2018 28
09-10-2018 29
Effect of seed hardening in micro nutrients of sunflower (Muthuvel et
al.,1983)
Treatment Seed yield
kgha
Oil yield kgha Seed yield
kgha
Oil yield kgha
Water 1054 446 1252 552
2% ZnSO4 1195 488 1531 673
1% MnSO4 1160 482 1314 597
09-10-2018 30
Seed hardening using botanical leaf in green gram
Treatment Plant
height
Days to
50%
flowering
Pod length No of
seedpod
Yieldha
Control 37.00 40 5.20 7 616.30
Prosopis
1%
45.73 37 6.52 10 758.03
Pungam
1%
44.70 37 6.35 9 727.73
Neem 1% 39.83 38 5.52 9 680.30
Aduthodai
ilai 1%
40.60 36 5.64 9 686.83
09-10-2018 31
Seed hardening on finger millet ( Palanisamy and Punithavathi)
Treatment Germination
%
Root length
cm
Shoot length
cm
Control 82 7.4 5.6
Water 87 7.3 5.8
KCl 1% 86 8.2 6.2
KCl + pungam
leaf extract
93 9.5 8.3
09-10-2018 32
Effect of seed hardening on wheat ( Shivamurthy and Patil,2009)
Treatment Plant
height
cm
Straw
yield
kgha
Protein
content
%
Total
chlorophyll
mgg
Net
return
Rs.ha
CaCl͙2 72.94 3261 12.21 2.53 9039
Cow urine 74.12 3388 12.17 2.43 9471
water 68.60 3063 11.89 2.22 7927
control 66.90 2893 11.71 2.15 6924
09-10-2018 33
SUMMARY
09-10-2018 34
Seed hardening is physiological
preconditioning of the seed by
hydration to with stand drought under
rain fed condition.
09-10-2018 35
DRY SEED
Soaking in water/dilute solutions of chemicals
and growth regulators for 1-12hrs at 15-25°c
SHADE DRY (1-24HRS)
Sun drying (1-2 days) to bring back to its
original water content
HARDENED SEED
09-10-2018 36
BENEFITS
Rapid and uniform germination.
Rapid growth rate of seedlings.
Recover much more quickly from wilting.
Seed withstand higher temperature for prolonged
period.
Compete more efficiently with weed.
09-10-2018 37
LIMITATIONS
Time
Concentration
Type
09-10-2018 38
09-10-2018 39
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09-10-2018 42

Seed hardening

  • 1.
  • 2.
    VENKATESH R B.Sc. AGRICULTURE ANBILDHARMALINGAM AGRICULTURAL COLLEGE AND RESEARCH INSTITUTE TRICHY-9 09-10-2018 2
  • 3.
  • 4.
  • 5.
  • 6.
  • 7.
    Poor quality seed Soilmoisture deficit Low and erratic rainfall Improper crop management 09-10-2018 7
  • 8.
  • 9.
    McDonald (2000) ,“Any post harvest treatment that improves germination or seedling emergence or facilitate the development of more number of normal, rapid, uniform and healthy seedlings in the field conditions.” 09-10-2018 9
  • 10.
  • 11.
  • 12.
    Henckel (1964) -“simple method to alter the physiological and biochemical nature of the seed in order to induce the factors responsible for drought resistance”. 09-10-2018 12
  • 13.
    It can bedone with water / dilute chemical solutions / growth regulating compounds or using commonly available natural tonics like coconut water or milk. 09-10-2018 13
  • 14.
    CHEMICALS ROLE Potassium nitrateOsmo-regulation, cell wall and membrane permeability Potassium chloride Activation of enzyme process Potassium di hydrogen phosphate Constituents of phospholipids, nucleotides and sugar phosphate Calcium chloride Participate in starch metabolism 09-10-2018 14
  • 15.
    09-10-2018 15 BOTANICALS ROLE PongamBio enhancement due to presence of plant growth hormones Prosopis Stimulatory effects caused by plant growth hormones Moringa Stimulatory effects caused by plant growth hormones Pulse sprout extract Induction of sugars and vitamins that promotes growth
  • 16.
    09-10-2018 16 MICROBIAL ENHANCERSROLE Rhizobium Induction of root nodulation Azatobacter Source of PGR Trichoderma viridi Fungal antagonist
  • 17.
    PHYSIOLOGICAL REORGANIZATIONS Change inhydrophilic property of the protoplasmic colloids namely the viscosity and elasticity. Increase in osmotic potential. Increase in water holding capacity of plants. 09-10-2018 17
  • 18.
    METABOLIC CHANGES Increased respiration. Leavesof hardened plants have more starch. Increased activity of the mitochondria. Higher rate of photosynthesis because of increase in the bound water. 09-10-2018 18
  • 19.
    MORPHOLOGICAL CHANGES More xeromorphicmorphology. Epidermal and stomata cells are smaller. Foliage area is increased. Faster recovery from atmospheric drought. More absorbing surface in the root system, as well as more number of primary roots. 09-10-2018 19
  • 20.
  • 21.
    DRY SEED SOAKING INWATER IMBITION PERMITS INITIAL GERMINATION PROCESS DEHYDRATION PREVENT RADICLE EMERGENCE SHADE DRY STOPS GERMINATION PROCESS HARDENED SEED SOWING IN DRY LAND GERMINATION STARTS FROM WHERE IT STOPPED RAPID ESTABLISHMENT,GROWTH AND YIELD 09-10-2018 21
  • 22.
  • 23.
  • 24.
  • 25.
    Crop Chemical concentrationSoaking duration (hrs.) Seed solution ratio Maize,varagu,tenai,samai KH2PO4 2% 8 1:0.6 Pearl millet KCl 2% 10 1:0.6 Sorghum KH2PO4 2% 16 1:0.6 Ragi NaCl 0.2% 6 1:0.7 Cotton KCl 2% 16 1:0.5 Sunflower KCl 2% 12 1:0.6 Red gram ZnSO4 100 ppm 3 1:0.3 Black gram ZnSO4 100 ppm 3 1:0:3 Ground nut CaCl 0.5 % 4 1:0.3 Paddy KCl 1% 16 1:0.6 09-10-2018 25
  • 26.
  • 27.
  • 28.
    Effect of seedhardening in Bt. cotton Treatment Germination Chlorophyll content mgg No of bollsplant Yield kgha CaCl21% 89.7 2.52 31 2518 CaCl2 2% 93.0 3.04 33 2629 CCC 100PPM 91.3 2.62 31 2557 CCC 150PPM 88.7 2.46 29 2492 WATER 84.7 2.15 26 2338 CONTROL 82.7 11.98 25 2276 09-10-2018 28
  • 29.
  • 30.
    Effect of seedhardening in micro nutrients of sunflower (Muthuvel et al.,1983) Treatment Seed yield kgha Oil yield kgha Seed yield kgha Oil yield kgha Water 1054 446 1252 552 2% ZnSO4 1195 488 1531 673 1% MnSO4 1160 482 1314 597 09-10-2018 30
  • 31.
    Seed hardening usingbotanical leaf in green gram Treatment Plant height Days to 50% flowering Pod length No of seedpod Yieldha Control 37.00 40 5.20 7 616.30 Prosopis 1% 45.73 37 6.52 10 758.03 Pungam 1% 44.70 37 6.35 9 727.73 Neem 1% 39.83 38 5.52 9 680.30 Aduthodai ilai 1% 40.60 36 5.64 9 686.83 09-10-2018 31
  • 32.
    Seed hardening onfinger millet ( Palanisamy and Punithavathi) Treatment Germination % Root length cm Shoot length cm Control 82 7.4 5.6 Water 87 7.3 5.8 KCl 1% 86 8.2 6.2 KCl + pungam leaf extract 93 9.5 8.3 09-10-2018 32
  • 33.
    Effect of seedhardening on wheat ( Shivamurthy and Patil,2009) Treatment Plant height cm Straw yield kgha Protein content % Total chlorophyll mgg Net return Rs.ha CaCl͙2 72.94 3261 12.21 2.53 9039 Cow urine 74.12 3388 12.17 2.43 9471 water 68.60 3063 11.89 2.22 7927 control 66.90 2893 11.71 2.15 6924 09-10-2018 33
  • 34.
  • 35.
    Seed hardening isphysiological preconditioning of the seed by hydration to with stand drought under rain fed condition. 09-10-2018 35
  • 36.
    DRY SEED Soaking inwater/dilute solutions of chemicals and growth regulators for 1-12hrs at 15-25°c SHADE DRY (1-24HRS) Sun drying (1-2 days) to bring back to its original water content HARDENED SEED 09-10-2018 36
  • 37.
    BENEFITS Rapid and uniformgermination. Rapid growth rate of seedlings. Recover much more quickly from wilting. Seed withstand higher temperature for prolonged period. Compete more efficiently with weed. 09-10-2018 37
  • 38.
  • 39.
  • 40.
  • 41.
  • 42.