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VENKATESH R
B.Sc. AGRICULTURE
ANBIL DHARMALINGAM AGRICULTURAL COLLEGE AND RESEARCH
INSTITUTE
TRICHY-9
ABILITY OF MICROORGANISMS TO CONVERT INEXPENSIVE RAW
MATERIALS OR SUBSTRATES TO ECONOMICALLY VALUABLE ORGANIC
COMPOUNDS.
INDUSTRIAL
MICROBIOLOGY
PHARMACEUTIC
ALS
FOOD
SANITARY
AGRICULTUR
AL
ACTIVITIES OF INDUSTRIAL MICROBIOLOGY
• Isolation, description,
• Culture conditions,
• Performing the fermentations,
• The Recovery,
• Chemical purification,
• Packing and marketing.
PRODUTS OF MICROBIAL ACTIVITY
• BIO CONVERSION PRODUCTS:
Beverages
Food
• METABOLITES: Intracellular and extracellular compounds
elaborated by the organisms.
• CELL BIOMASS: Microbial cells themselves act as a food
ANTIBIOTICS-PENICILLINS
ORGANIC ACIDS-GIBBERELLIN ACID
 Gibberellin fujikuroi-fungus
 Bacterial species of Rhizobium.
 Medium-Glucose 2%,MgSo4 0.3%,soyabean flour+ground nut.
 Incubation tempt 25-30℃’.First stage 72 hrs.(10-15old
culture).second stage 48hrs.
 pH 6.0-6.5,aerobic,Fermentation-7 days.
 GA3 is produced.(90 types)
VITAMINS
vitamins organisms media fermentation
s
yield
B2 Ashby a
gossyppi
Glucose,
Soya oil,
Glycine.
6days,
36℃,
Aerobic.
4.25g/l
B12 Propionibacte
rium
shermanii
Glucose,
Corn steep,
Ammonia,
Cobalt,
pH 7.0.
3days,
30℃,
Anaerobic.
(aerobic 4
days)
23mg/l
ALCOHOLIC BEVERAGES-WINE
saccharomyces cerevisiae var.ellipsoideus malolactic
fermentation
Grapes SO2 yeast
must alcoholic press settling vat
fermentation
RED =5-10=70-90℃
WHITE=10-15=55-60℃ filtration
bottling(wood oak) aging(stainless air tight)
Malic
acid-
lactic
acid
3-
6mont
hs
DAIRY INDUSTRY-YOGURF
• Turkish word(fermented milk).
• Conditioning the milk, heated to 86-93˚c for 30-60 mints.
• Cooling to 45˚c,streptococcus thermophilus,lactobacillus
bulgaricus is added
• Fermentation is at 45˚c for 3-5 hrs.
• Stored at 0-4˚c until consumed to prevent spoilage.
FERMENTED CEREAL PRODUCTS
• IDLI-Rice +black gram(3:1/4:1)
• Soaking for 3-10hrs &ground to paste
• Fermentation 10-12hrs at tempt 25-30˚c.
• Leuconostoc mesentrroids,pediococcus cerevisiae,streptococcus
faecalis bacteria occurs.
• Fermentation lactic acid &acetic acid gets accumulated, the
batter becomes soured.
• Metals can be extracted from
low grade sulphide containing
ores using Thiobacillus Ferro
oxidants.
• Rhizopus species binds
uranium from lower grade
ores and nuclear wastes
• This type of recovery is called
bio leaching.
SUGAR
bacterial enzyme
GLYCEROL
yeast enzyme
TRIMETHYLENE GLYCOL (3G)
polymerized
POLYSTER
Bacteria can produce both cotton
&fabric .
OTHER FIELDS
FABRIC-polyster RECOVERY OF MINERALS
FIELD MICROORGANISMS PRODUCT
PRODUCTION OF FUELS Methanogenic bacteria
Citrobactor sp
Methane
Hydrogen(also by -product
of microbial fermentation)
Recovery of oil Xanthomonas campestris
Bacteria(xanthan gums)
In petroleum wells to force
out oil in small pores in the
rock layers.
Bio sensors E-coli(luxoperon) To detect hazardous
chemicals in soil &water
Paper Gluconacetobacter(cellulos
e)
E-paper
Computer ,Robert Berge
“Syracuse university’s
center of molecular
electronics”
Halo bacterium
(bacteriorhodopsin)
Computer chip,
Manufacturing salt around
san Francisco Bay
Microbial polyhydroxy
alkanoates(PHA)
E-coli(recombinant DNA
technology)
Bio degradable plastics
REFERENCES
• INDUSTRIAL MICROBIOLOGY - Dr.K.G.ANITHA
• GENERA MICROBIOLOGY - S.B.SULLIA &
S.SHANTHANAM
• INDUSTRIAL MICROBIOLOGY - A.H.PATEL
• MICROBIOLOGY –AN INDRODUCTION 9th
EDITION-PEARSON
THANK YOU

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Microorganisms Convert Raw Materials into Valuable Compounds

  • 1.
  • 2. VENKATESH R B.Sc. AGRICULTURE ANBIL DHARMALINGAM AGRICULTURAL COLLEGE AND RESEARCH INSTITUTE TRICHY-9
  • 3. ABILITY OF MICROORGANISMS TO CONVERT INEXPENSIVE RAW MATERIALS OR SUBSTRATES TO ECONOMICALLY VALUABLE ORGANIC COMPOUNDS. INDUSTRIAL MICROBIOLOGY PHARMACEUTIC ALS FOOD SANITARY AGRICULTUR AL
  • 4. ACTIVITIES OF INDUSTRIAL MICROBIOLOGY • Isolation, description, • Culture conditions, • Performing the fermentations, • The Recovery, • Chemical purification, • Packing and marketing.
  • 5. PRODUTS OF MICROBIAL ACTIVITY • BIO CONVERSION PRODUCTS: Beverages Food • METABOLITES: Intracellular and extracellular compounds elaborated by the organisms. • CELL BIOMASS: Microbial cells themselves act as a food
  • 7. ORGANIC ACIDS-GIBBERELLIN ACID  Gibberellin fujikuroi-fungus  Bacterial species of Rhizobium.  Medium-Glucose 2%,MgSo4 0.3%,soyabean flour+ground nut.  Incubation tempt 25-30℃’.First stage 72 hrs.(10-15old culture).second stage 48hrs.  pH 6.0-6.5,aerobic,Fermentation-7 days.  GA3 is produced.(90 types)
  • 8. VITAMINS vitamins organisms media fermentation s yield B2 Ashby a gossyppi Glucose, Soya oil, Glycine. 6days, 36℃, Aerobic. 4.25g/l B12 Propionibacte rium shermanii Glucose, Corn steep, Ammonia, Cobalt, pH 7.0. 3days, 30℃, Anaerobic. (aerobic 4 days) 23mg/l
  • 9. ALCOHOLIC BEVERAGES-WINE saccharomyces cerevisiae var.ellipsoideus malolactic fermentation Grapes SO2 yeast must alcoholic press settling vat fermentation RED =5-10=70-90℃ WHITE=10-15=55-60℃ filtration bottling(wood oak) aging(stainless air tight) Malic acid- lactic acid 3- 6mont hs
  • 10. DAIRY INDUSTRY-YOGURF • Turkish word(fermented milk). • Conditioning the milk, heated to 86-93˚c for 30-60 mints. • Cooling to 45˚c,streptococcus thermophilus,lactobacillus bulgaricus is added • Fermentation is at 45˚c for 3-5 hrs. • Stored at 0-4˚c until consumed to prevent spoilage.
  • 11. FERMENTED CEREAL PRODUCTS • IDLI-Rice +black gram(3:1/4:1) • Soaking for 3-10hrs &ground to paste • Fermentation 10-12hrs at tempt 25-30˚c. • Leuconostoc mesentrroids,pediococcus cerevisiae,streptococcus faecalis bacteria occurs. • Fermentation lactic acid &acetic acid gets accumulated, the batter becomes soured.
  • 12. • Metals can be extracted from low grade sulphide containing ores using Thiobacillus Ferro oxidants. • Rhizopus species binds uranium from lower grade ores and nuclear wastes • This type of recovery is called bio leaching. SUGAR bacterial enzyme GLYCEROL yeast enzyme TRIMETHYLENE GLYCOL (3G) polymerized POLYSTER Bacteria can produce both cotton &fabric . OTHER FIELDS FABRIC-polyster RECOVERY OF MINERALS
  • 13. FIELD MICROORGANISMS PRODUCT PRODUCTION OF FUELS Methanogenic bacteria Citrobactor sp Methane Hydrogen(also by -product of microbial fermentation) Recovery of oil Xanthomonas campestris Bacteria(xanthan gums) In petroleum wells to force out oil in small pores in the rock layers. Bio sensors E-coli(luxoperon) To detect hazardous chemicals in soil &water Paper Gluconacetobacter(cellulos e) E-paper Computer ,Robert Berge “Syracuse university’s center of molecular electronics” Halo bacterium (bacteriorhodopsin) Computer chip, Manufacturing salt around san Francisco Bay Microbial polyhydroxy alkanoates(PHA) E-coli(recombinant DNA technology) Bio degradable plastics
  • 14. REFERENCES • INDUSTRIAL MICROBIOLOGY - Dr.K.G.ANITHA • GENERA MICROBIOLOGY - S.B.SULLIA & S.SHANTHANAM • INDUSTRIAL MICROBIOLOGY - A.H.PATEL • MICROBIOLOGY –AN INDRODUCTION 9th EDITION-PEARSON