Rocket Launcher Mechanism of Collaborative Actin Assembly Defined by Single-Molecule Imaging
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This document summarizes a presentation on Adenomanous Polypolis Coli (APC) and its role in the Wnt signaling pathway and actin assembly. APC is a main component of the Wnt signaling pathway that regulates β-catenin levels and target gene expression. Mutations in APC are associated with colorectal cancers. The presentation shows that APC can directly nucleate actin assembly through its C-terminal basic domain, without sequence homology to known actin binding domains. APC and mDia1 synergistically promote actin assembly in the presence of profilin and capping protein. While the formin mDia1 remains processively bound to actin filament bar
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The document summarizes research presented on the role of Adenomanous Polyposis Coli (APC) in regulating actin assembly. APC is a key component of the Wnt signaling pathway and acts as a tumor suppressor. When mutations occur in the APC gene, it can lead to familial adenomatous polyposis. Research shows that APC directly nucleates actin assembly through its C-terminal basic domain without any known actin-binding motifs. Studies using fluorescence probes indicate that unlike other actin nucleators like Arp2/3 or formins, APC remains bound to the pointed end of actin filaments during nucleation and elongation.
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The document discusses the structure and replication of DNA. It describes DNA as being made up of nucleotides, which consist of a sugar, phosphate, and one of four nitrogenous bases. The two strands of DNA are held together in the iconic double helix structure by hydrogen bonds between the bases, with adenine bonding only with thymine and cytosine bonding only with guanine. DNA replication involves enzymes unwinding the double helix, and DNA polymerase then synthesizing new matching strands by adding complementary bases to each template strand from 5' to 3' according to the base-pairing rules, thus replicating the genetic information.
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The genetic code is the sequence of nitrogen bases in mRNA that contains the information for protein synthesis. A codon is three nitrogen bases that code for a single amino acid. Nirenberg and Mathaei experimentally proved that codons determine amino acids. The genetic code is universal, uses non-overlapping triplets to specify amino acids in a linear, commaless fashion, and employs initiation and termination codons.
Genetic code
1) There are three main types of RNA - mRNA carries genetic information from DNA to ribosomes, tRNA transfers amino acids during protein synthesis, and rRNA makes up the ribosomes.
2) Transcription copies one DNA strand into mRNA which moves to ribosomes for translation. During translation, tRNAs match their anticodons to mRNA codons and add the corresponding amino acids.
3) Mutations in DNA can lead to production of defective proteins through altered mRNA and incorrect amino acid sequences, resulting in genetic diseases.
Chapter 11 Notes - Biology I
DNA contains the genetic instructions used in the development and functioning of all living organisms. It is made up of nucleotides with a phosphate group, sugar, and one of four nitrogenous bases. DNA replicates through the process of DNA replication in which the double helix unwinds and enzymes add complementary bases to each strand. During protein production, information from DNA is transcribed into messenger RNA (mRNA) which is then translated by ribosomes to produce proteins made of amino acid chains. Mutations can occur through changes in single base pairs or the structure of chromosomes and can be caused by mutagens like radiation, chemicals, or heat.
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Genetic code -
The genetic code is the system by which nucleotide sequences in mRNA determine the amino acid sequences in proteins. The genetic code uses triplets of nucleotides called codons to specify which amino acid will be incorporated into the growing polypeptide chain. There are 64 possible codons but only 20 standard amino acids, so most amino acids have multiple codons. Three codons act as stop signals to end protein synthesis. The genetic code is nearly universal across all life due to its high degree of specificity and redundancy.
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The document summarizes research presented on the role of Adenomanous Polyposis Coli (APC) in regulating actin assembly. APC is a key component of the Wnt signaling pathway and acts as a tumor suppressor. When mutations occur in the APC gene, it can lead to familial adenomatous polyposis. Research shows that APC directly nucleates actin assembly through its C-terminal basic domain without any known actin-binding motifs. Studies using fluorescence probes indicate that unlike other actin nucleators like Arp2/3 or formins, APC remains bound to the pointed end of actin filaments during nucleation and elongation.
Structure of dna and replication2012
The document discusses the structure and replication of DNA. It describes DNA as being made up of nucleotides, which consist of a sugar, phosphate, and one of four nitrogenous bases. The two strands of DNA are held together in the iconic double helix structure by hydrogen bonds between the bases, with adenine bonding only with thymine and cytosine bonding only with guanine. DNA replication involves enzymes unwinding the double helix, and DNA polymerase then synthesizing new matching strands by adding complementary bases to each template strand from 5' to 3' according to the base-pairing rules, thus replicating the genetic information.
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Genetic code - Charateristics
The genetic code is the sequence of nitrogen bases in mRNA that contains the information for protein synthesis. A codon is three nitrogen bases that code for a single amino acid. Nirenberg and Mathaei experimentally proved that codons determine amino acids. The genetic code is universal, uses non-overlapping triplets to specify amino acids in a linear, commaless fashion, and employs initiation and termination codons.
Genetic code
1) There are three main types of RNA - mRNA carries genetic information from DNA to ribosomes, tRNA transfers amino acids during protein synthesis, and rRNA makes up the ribosomes.
2) Transcription copies one DNA strand into mRNA which moves to ribosomes for translation. During translation, tRNAs match their anticodons to mRNA codons and add the corresponding amino acids.
3) Mutations in DNA can lead to production of defective proteins through altered mRNA and incorrect amino acid sequences, resulting in genetic diseases.
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DNA contains the genetic instructions used in the development and functioning of all living organisms. It is made up of nucleotides with a phosphate group, sugar, and one of four nitrogenous bases. DNA replicates through the process of DNA replication in which the double helix unwinds and enzymes add complementary bases to each strand. During protein production, information from DNA is transcribed into messenger RNA (mRNA) which is then translated by ribosomes to produce proteins made of amino acid chains. Mutations can occur through changes in single base pairs or the structure of chromosomes and can be caused by mutagens like radiation, chemicals, or heat.
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2. tRNA molecules carry specific amino acids and have anticodons that are complementary to mRNA codons, allowing the correct amino acid to be added to the growing polypeptide chain via the ribosome.
3. Translation is the process of protein synthesis, directed by the mRNA template, where tRNA molecules bring amino acids to the ribosome according to codon-anticodon base pairing. The amino acids are linked together through the joining of peptide bonds.
Wobble hypothesis
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The document describes the process of DNA replication. It begins with DNA unwinding at the origin of replication site, where helicase enzymes cause the double helix to separate. Free nucleotides then base pair with the exposed, complementary bases on each single strand. DNA polymerase joins the nucleotides to form new polynucleotide chains. Finally, the two new DNA molecules each contain one original and one new strand, and the double helix reforms.
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The genetic code is composed of triplets of nucleotide bases that correspond to specific amino acids. There are 64 possible codon combinations from sequences of the 4 nucleotide bases, with 61 coding for 20 amino acids and 3 serving as stop codons. The genetic code is universal across all living organisms, specifying the same amino acids for each codon. It is read in sets of 3 bases moving in the 5' to 3' direction on mRNA, and mutations in the code can result in silent, missense, nonsense, or frameshift changes to the specified protein.
Replication
DNA replication is semi-conservative, as proved by the Meselson-Stahl experiment. It occurs during interphase and involves unwinding of the DNA double helix by helicase, followed by synthesis of new strands by DNA polymerases with RNA primers provided by primase. Replication proceeds bidirectionally from an origin of replication and results in two identical copies of the original DNA molecule.
Central dogma of life
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1. The genetic code is deciphered through research that matched codons (three-nucleotide sequences in mRNA) to their corresponding amino acids. It was discovered that 61 codons code for specific amino acids while 3 are stop codons.
2. tRNA molecules carry specific amino acids and have anticodons that are complementary to mRNA codons, allowing the correct amino acid to be added to the growing polypeptide chain via the ribosome.
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DNA replication is semi-conservative, as proved by the Meselson-Stahl experiment. It occurs during interphase and involves unwinding of the DNA double helix by helicase, followed by synthesis of new strands by DNA polymerases with RNA primers provided by primase. Replication proceeds bidirectionally from an origin of replication and results in two identical copies of the original DNA molecule.
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Very recent work shows that SARS-CoV-2 enters our cells through receptor-mediated endocytosis, dependent on an endosomal bafilomycin-sensitive proton pump as well as two-pore channels (TPCs). Physiological intracellular Ca2+ signals, mediated by the messenger nicotinic acid adenine dinucleotide phosphate (NAADP), depend on the very same proton pump and TPCs. Two hitherto completely separate research fields, namely molecular virology and cellular Ca2+ signaling physiology are now coming together, creating exciting new research opportunities.
Trafficking of Endosomes and Phagosomes: Geometry, Force and Cholesterol
Roop Mallik, PhD
Uptake of material from the external world by endocytosis/phagocytosis supplies nutrients to cells, and is also critical for cell signaling. The journey of endosomes/phagosomes begins at the cell periphery and ends at lysosomes near the cell center. I will discuss how the balance of forces generated by antagonistic motor proteins guides this journey, and how lipids are emerging as a master-controller of this balance.
Calcium Buffering in Endo- and Exocytosis Studies
Erwin Neher, FRS
Researchers use calcium-chelators (buffers) to manipulate levels of free intracellular calcium concentration ([Ca2+]i) and to shape Calcium signals. Unlike pH buffers, which are used to strictly control pH levels, calcium buffers inside a living cell may not influence the steady-state level of [Ca2+]i at all, but rather slow-down [Ca2+]i-changes induced either endogenously or by the experimenter. Such properties and their consequences on Ca2+-imaging will be discussed.
Cataract.
This document provides an overview of cataracts, including their anatomy, classification, pathophysiology, genetics, biochemical pathways, tests and treatments, prevalence, and references. It describes the key structures of the eye involved in cataracts such as the iris, pupil, sclera, cornea, and retina. It classifies cataracts based on location, age of onset, morphology, and maturity. The main pathophysiological mechanisms and risk factors are discussed. Genetic inheritance patterns and candidate genes are outlined. Biochemical pathways like glycolysis, the pentose phosphate shunt, and the polyol route are implicated in cataract formation. Tests, treatments, and prevalence statistics in Pakistan are summarized along with references
The Role Of The Cytoskeleton During Neuronal Polarization
This document summarizes recent advances in understanding the intracellular mechanisms underlying neuronal polarization, with a focus on the role of the cytoskeleton. It discusses how local actin instability in the future axonal growth cone allows microtubule protrusion and formation of multiple axons. Key regulators of this process include Rho-GTPases and their downstream targets, which influence actin dynamics. The Ena/VASP family of proteins also promotes neurite formation by antagonizing actin capping and facilitating bundling. Recent studies further reveal microtubule stabilization as another mechanism in neuronal polarization, complementing actin dynamics.
Role of Vangl2 in Female Reproductive Tract Development, 2008
Postdoctoral work on Vangl2, a protein involved in cell polarity. Cell polarity is the capability of a cell to be able to orient itself in a particular direction.
Canavanine Background
Canavanine, found in alfalfa seeds, functions as an analogue to arginine in yeast cells. It enters yeast cells via the arginine permease and becomes incorporated into proteins by the arginine tRNA synthetase instead of arginine. This misfolding of proteins due to canavanine incorporation leads to cell death. However, UV radiation can induce mutations in the CAN1 gene, which codes for the arginine permease. A mutation in this gene prevents canavanine from entering the cell, allowing the cell to survive by avoiding protein misfolding. The rate of these beneficial mutations is higher under UV radiation than without it.
Stephen Friend ICR UK 2012-06-18
The document discusses exploring disease networks and how science is performed through networked approaches. It describes how understanding disease requires integrating DNA, RNA, protein and molecular networks. It highlights the EGFR pathway example to show biomarkers can predict treatment response complexity. CETP inhibition example shows causal relationships are not always correlative. Networked approaches are needed to generate, analyze and support new models through data sharing to help understand disease mechanisms and save costs. Synapse is proposed as a platform to enable open sharing of clinical and genomic data as well as model building, similar to how software development occurs through tools like GitHub.
Okayama nakanishi 114.003
This document discusses research on marrow stromal cells and their potential applications for regenerative medicine and tissue engineering. Key points include:
1. Marrow stromal cells reside in the bone marrow microenvironment and can differentiate into various cell types, including bone, cartilage, fat, and muscle cells.
2. Studies show marrow stromal cells implanted in rodents generate new bone tissue in vivo.
3. The document proposes strategies for controlling the shape of new bone tissue using biodegradable polymer scaffolds seeded with osteoblasts or chondrocytes.
4. Methods are presented for selecting progenitor cells using cell surface markers and gene regulatory elements to target cell differentiation for tissue engineering applications
approach to congenital cataract.pptx
This document discusses lens anatomy and development, as well as congenital cataracts. It notes that the lens develops from surface ectoderm, not neuroectoderm, and that crystallins make up 90% of lens proteins. Congenital cataracts can be classified based on morphology, laterality, progression, and genetic or syndromic associations. Evaluation of a congenital cataract case includes family history, examination of acuity and ocular structures, laboratory tests, and potentially genetic testing. Treatment involves surgery, with timing depending on density and progression of the cataract.
Similar to Rocket Launcher Mechanism of Collaborative Actin Assembly Defined by Single-Molecule Imaging (20)
10. Skeleton and Cell movement, actin - cell biology
10. Skeleton and Cell movement, actin - cell biology
The International Journal of Engineering and Science (IJES)
The International Journal of Engineering and Science (IJES)
Endocytosis and Endosome Trafficking: Roles in Coronavirus Uptake and Cell Si...
Endocytosis and Endosome Trafficking: Roles in Coronavirus Uptake and Cell Si...
The Role Of The Cytoskeleton During Neuronal Polarization
The Role Of The Cytoskeleton During Neuronal Polarization
Role of Vangl2 in Female Reproductive Tract Development, 2008
Role of Vangl2 in Female Reproductive Tract Development, 2008
More from Suk Namgoong
Cell bio8
The document discusses the cell cycle and its regulation. It begins by outlining the four main phases: G1, S, G2, and M. Checkpoints exist to monitor DNA damage and proper chromosome attachment before transitions. Research led to the discovery of cyclins and cyclin-dependent kinases (CDKs) that control cell cycle progression. Specifically, maturation promoting factor (MPF), composed of cyclin B and CDK1, induces mitosis. Protein destruction via the anaphase promoting complex (APC) also regulates the cell cycle. Growth and DNA integrity checkpoints in G1 determine whether a cell divides.
Cell bio3
This document provides an overview of the topics covered in an advanced cell biology course, including the central dogma of molecular biology, nuclear structure and function, nuclear transport, and chromosome structure. The key topics are:
1) DNA replication, transcription, and splicing occur in the nucleus, where the nuclear envelope, nuclear pore complexes, lamina, and nucleolus are located.
2) Nuclear pore complexes act as gateways between the nucleus and cytoplasm, regulating nuclear transport of molecules through nuclear localization and export signals.
3) Chromosome structure involves packaging of DNA into nucleosomes and further into chromatin, with regions of euchromatin and heterochromatin that are differentially transcribed.
Cell Biology Lecture 3
This document provides an overview of a course on advanced cell biology. It focuses on the topic of membranes, including their structure and key components. The main points are:
- Biological membranes act as barriers that compartmentalize the cell and contain lipids, integral proteins, and peripheral proteins.
- The fluid mosaic model describes membranes as a fluid bilayer of lipids with integral proteins embedded. Key lipids include phospholipids and sphingolipids. Cholesterol provides rigidity.
- Integral membrane proteins span the membrane and include transmembrane alpha helices. Their structure can be predicted using hydropathy plots.
- Membranes establish gradients using pumps, carriers, and channels. Pumps actively transport ions using ATP.
Crispr/cas9 101
This document provides an overview of CRISPR/Cas9 genome editing. It discusses the history and limitations of prior genome engineering techniques like recombinant DNA and zinc finger nucleases. It then explains how CRISPR/Cas9 works as a RNA-guided DNA endonuclease and how this allows it to efficiently and specifically edit genomes. The document outlines several applications of CRISPR/Cas9 like generating knockout animals and cell lines. It also notes some concerns about using the technique for human genome editing.
Cell biology
This document provides information about an advanced cell biology course including the lecturer's contact details, textbook, course objectives, topics, and syllabus. The course will cover basic cell components and structures like the nucleus, membrane, cytoskeleton, and cell cycle. It will focus on the molecular basis of life at the cellular level and general principles and mechanisms. The first week will be an introduction and subsequent weeks will cover specific topics like transcription, signaling, and trafficking. Cells are introduced as the basic functional units of living organisms and their evolution is briefly outlined.
Cell culture 07
This document appears to be the syllabus for a 7th lecture course on Advanced Animal Cell Culture offered in the 2nd semester of 2013 at Chungbuk National University's Department of Animal Science. The syllabus lists 8 topics that will be covered across 8 class dates from September to December 2013. These topics include introductions to cell culture, using cell culture as a research model, antibody/protein production in cell culture, protein production and purification, stem cells, and generating transgenic/knockout animals and genome engineering techniques. It also lists which students will present on certain topics and dates.
Cell culture 06
This document contains the syllabus for an advanced animal cell culture course from the Department of Animal Science at Chungbuk National University. The syllabus lists 8 topics that will be covered between September 2013 and December 2013, including introductions to cell culture and stem cells, using cell culture for antibody and protein production, transgenic and knockout animals, and genome engineering. Student groups are assigned to present on specific dates, including topics like stem cell I and II and protein production/purification. The document also includes an excerpt from the November 14th lecture on stem cell II, discussing the mechanisms of cellular reprogramming and how induced pluripotent stem cells were discovered.
Cell culture 04
This document appears to be the syllabus for a course titled "Advanced Animal Cell Culture" offered in the second semester of 2013 at Chungbuk National University's Department of Animal Science. The syllabus lists 8 topics that will be covered across 8 class dates from September to December. These topics include introductions to cell culture, using cell culture as a research model, producing antibodies and proteins through cell culture, protein production and purification techniques, and stem cell research. The document also provides details on assignments and presentations related to specific class dates.
Cell culture 03
This document contains the syllabus for an advanced animal cell culture course offered in the second semester of 2013 at Chungbuk National University's Department of Animal Science. The syllabus lists 8 topics that will be covered across 8 classes between September and December, including introductions to cell culture, stem cells, transgenic animals, and genome engineering. It also provides the dates and instructors for the classes on cell culture for antibody and protein production, and protein production/purification.
Advanced Cell Culture Lecture 2 - CBNU
This document outlines the syllabus for an Advanced Animal Cell Culture course. It lists the topics that will be covered in each class, including introductions to cell culture, stem cells, transgenic and knockout animals, and genome engineering. It also provides details about some of the topics, such as commonly used cell lines and model organisms, cell culture techniques like subculture and cryopreservation, and research methods using cultured cells like microscopy, staining, and molecular biology techniques. The document emphasizes that cultured cells can serve as useful model systems to study specific cell types and biological phenomena while avoiding complications from whole organisms.
Advanced Cell Culture Lecture 1 - CBNU
This document provides information about an advanced animal cell culture course. It includes details about the course such as the lecturer, meeting times, grading structure, and syllabus. The syllabus covers topics like cell culture models, protein production, stem cells, transgenic and knockout animals, and genome engineering. It also provides background on why animal cells are cultured, the history of cell culture, types of primary and established cell lines, cell culture techniques like adherent and suspension cultures, and uses of cell culture including basic research, toxicity screening, protein production, and tissue engineering. Students in the course will present papers during seminars.
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Building Production Ready Search Pipelines with Spark and Milvus
Spark is the widely used ETL tool for processing, indexing and ingesting data to serving stack for search. Milvus is the production-ready open-source vector database. In this talk we will show how to use Spark to process unstructured data to extract vector representations, and push the vectors to Milvus vector database for search serving.
Essentials of Automations: The Art of Triggers and Actions in FME
In this second installment of our Essentials of Automations webinar series, we’ll explore the landscape of triggers and actions, guiding you through the nuances of authoring and adapting workspaces for seamless automations. Gain an understanding of the full spectrum of triggers and actions available in FME, empowering you to enhance your workspaces for efficient automation.
We’ll kick things off by showcasing the most commonly used event-based triggers, introducing you to various automation workflows like manual triggers, schedules, directory watchers, and more. Plus, see how these elements play out in real scenarios.
Whether you’re tweaking your current setup or building from the ground up, this session will arm you with the tools and insights needed to transform your FME usage into a powerhouse of productivity. Join us to discover effective strategies that simplify complex processes, enhancing your productivity and transforming your data management practices with FME. Let’s turn complexity into clarity and make your workspaces work wonders!
AI 101: An Introduction to the Basics and Impact of Artificial Intelligence
Imagine a world where machines not only perform tasks but also learn, adapt, and make decisions. This is the promise of Artificial Intelligence (AI), a technology that's not just enhancing our lives but revolutionizing entire industries.
Presentation of the OECD Artificial Intelligence Review of Germany
Consult the full report at https://www.oecd.org/digital/oecd-artificial-intelligence-review-of-germany-609808d6-en.htm
HCL Notes and Domino License Cost Reduction in the World of DLAU
Webinar Recording: https://www.panagenda.com/webinars/hcl-notes-and-domino-license-cost-reduction-in-the-world-of-dlau/
The introduction of DLAU and the CCB & CCX licensing model caused quite a stir in the HCL community. As a Notes and Domino customer, you may have faced challenges with unexpected user counts and license costs. You probably have questions on how this new licensing approach works and how to benefit from it. Most importantly, you likely have budget constraints and want to save money where possible. Don’t worry, we can help with all of this!
We’ll show you how to fix common misconfigurations that cause higher-than-expected user counts, and how to identify accounts which you can deactivate to save money. There are also frequent patterns that can cause unnecessary cost, like using a person document instead of a mail-in for shared mailboxes. We’ll provide examples and solutions for those as well. And naturally we’ll explain the new licensing model.
Join HCL Ambassador Marc Thomas in this webinar with a special guest appearance from Franz Walder. It will give you the tools and know-how to stay on top of what is going on with Domino licensing. You will be able lower your cost through an optimized configuration and keep it low going forward.
These topics will be covered
- Reducing license cost by finding and fixing misconfigurations and superfluous accounts
- How do CCB and CCX licenses really work?
- Understanding the DLAU tool and how to best utilize it
- Tips for common problem areas, like team mailboxes, functional/test users, etc
- Practical examples and best practices to implement right away
みなさんこんにちはこれ何文字まで入るの？40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの？えこ...
ここ3000字までしか入らないけどタイトルの方がたくさん文字入ると思います。
Best 20 SEO Techniques To Improve Website Visibility In SERP
Boost your website's visibility with proven SEO techniques! Our latest blog dives into essential strategies to enhance your online presence, increase traffic, and rank higher on search engines. From keyword optimization to quality content creation, learn how to make your site stand out in the crowded digital landscape. Discover actionable tips and expert insights to elevate your SEO game.
Infrastructure Challenges in Scaling RAG with Custom AI models
Building Retrieval-Augmented Generation (RAG) systems with open-source and custom AI models is a complex task. This talk explores the challenges in productionizing RAG systems, including retrieval performance, response synthesis, and evaluation. We’ll discuss how to leverage open-source models like text embeddings, language models, and custom fine-tuned models to enhance RAG performance. Additionally, we’ll cover how BentoML can help orchestrate and scale these AI components efficiently, ensuring seamless deployment and management of RAG systems in the cloud.
CAKE: Sharing Slices of Confidential Data on Blockchain
Presented at the CAiSE 2024 Forum, Intelligent Information Systems, June 6th, Limassol, Cyprus.
Synopsis: Cooperative information systems typically involve various entities in a collaborative process within a distributed environment. Blockchain technology offers a mechanism for automating such processes, even when only partial trust exists among participants. The data stored on the blockchain is replicated across all nodes in the network, ensuring accessibility to all participants. While this aspect facilitates traceability, integrity, and persistence, it poses challenges for adopting public blockchains in enterprise settings due to confidentiality issues. In this paper, we present a software tool named Control Access via Key Encryption (CAKE), designed to ensure data confidentiality in scenarios involving public blockchains. After outlining its core components and functionalities, we showcase the application of CAKE in the context of a real-world cyber-security project within the logistics domain.
Paper: https://doi.org/10.1007/978-3-031-61000-4_16
Artificial Intelligence for XMLDevelopment
In the rapidly evolving landscape of technologies, XML continues to play a vital role in structuring, storing, and transporting data across diverse systems. The recent advancements in artificial intelligence (AI) present new methodologies for enhancing XML development workflows, introducing efficiency, automation, and intelligent capabilities. This presentation will outline the scope and perspective of utilizing AI in XML development. The potential benefits and the possible pitfalls will be highlighted, providing a balanced view of the subject.
We will explore the capabilities of AI in understanding XML markup languages and autonomously creating structured XML content. Additionally, we will examine the capacity of AI to enrich plain text with appropriate XML markup. Practical examples and methodological guidelines will be provided to elucidate how AI can be effectively prompted to interpret and generate accurate XML markup.
Further emphasis will be placed on the role of AI in developing XSLT, or schemas such as XSD and Schematron. We will address the techniques and strategies adopted to create prompts for generating code, explaining code, or refactoring the code, and the results achieved.
The discussion will extend to how AI can be used to transform XML content. In particular, the focus will be on the use of AI XPath extension functions in XSLT, Schematron, Schematron Quick Fixes, or for XML content refactoring.
The presentation aims to deliver a comprehensive overview of AI usage in XML development, providing attendees with the necessary knowledge to make informed decisions. Whether you’re at the early stages of adopting AI or considering integrating it in advanced XML development, this presentation will cover all levels of expertise.
By highlighting the potential advantages and challenges of integrating AI with XML development tools and languages, the presentation seeks to inspire thoughtful conversation around the future of XML development. We’ll not only delve into the technical aspects of AI-powered XML development but also discuss practical implications and possible future directions.
“Building and Scaling AI Applications with the Nx AI Manager,” a Presentation...
“Building and Scaling AI Applications with the Nx AI Manager,” a Presentation...Edge AI and Vision Alliance
For the full video of this presentation, please visit: https://www.edge-ai-vision.com/2024/06/building-and-scaling-ai-applications-with-the-nx-ai-manager-a-presentation-from-network-optix/
Robin van Emden, Senior Director of Data Science at Network Optix, presents the “Building and Scaling AI Applications with the Nx AI Manager,” tutorial at the May 2024 Embedded Vision Summit.
In this presentation, van Emden covers the basics of scaling edge AI solutions using the Nx tool kit. He emphasizes the process of developing AI models and deploying them globally. He also showcases the conversion of AI models and the creation of effective edge AI pipelines, with a focus on pre-processing, model conversion, selecting the appropriate inference engine for the target hardware and post-processing.
van Emden shows how Nx can simplify the developer’s life and facilitate a rapid transition from concept to production-ready applications.He provides valuable insights into developing scalable and efficient edge AI solutions, with a strong focus on practical implementation.GraphRAG for Life Science to increase LLM accuracy
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Welcome to UiPath Test Automation using UiPath Test Suite series part 6. In this session, we will cover Test Automation with generative AI and Open AI.
UiPath Test Automation with generative AI and Open AI webinar offers an in-depth exploration of leveraging cutting-edge technologies for test automation within the UiPath platform. Attendees will delve into the integration of generative AI, a test automation solution, with Open AI advanced natural language processing capabilities.
Throughout the session, participants will discover how this synergy empowers testers to automate repetitive tasks, enhance testing accuracy, and expedite the software testing life cycle. Topics covered include the seamless integration process, practical use cases, and the benefits of harnessing AI-driven automation for UiPath testing initiatives. By attending this webinar, testers, and automation professionals can gain valuable insights into harnessing the power of AI to optimize their test automation workflows within the UiPath ecosystem, ultimately driving efficiency and quality in software development processes.
What will you get from this session?
1. Insights into integrating generative AI.
2. Understanding how this integration enhances test automation within the UiPath platform
3. Practical demonstrations
4. Exploration of real-world use cases illustrating the benefits of AI-driven test automation for UiPath
Topics covered:
What is generative AI
Test Automation with generative AI and Open AI.
UiPath integration with generative AI
Speaker:
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
Monitoring and Managing Anomaly Detection on OpenShift.pdf
Monitoring and Managing Anomaly Detection on OpenShift
Overview
Dive into the world of anomaly detection on edge devices with our comprehensive hands-on tutorial. This SlideShare presentation will guide you through the entire process, from data collection and model training to edge deployment and real-time monitoring. Perfect for those looking to implement robust anomaly detection systems on resource-constrained IoT/edge devices.
Key Topics Covered
1. Introduction to Anomaly Detection
- Understand the fundamentals of anomaly detection and its importance in identifying unusual behavior or failures in systems.
2. Understanding Edge (IoT)
- Learn about edge computing and IoT, and how they enable real-time data processing and decision-making at the source.
3. What is ArgoCD?
- Discover ArgoCD, a declarative, GitOps continuous delivery tool for Kubernetes, and its role in deploying applications on edge devices.
4. Deployment Using ArgoCD for Edge Devices
- Step-by-step guide on deploying anomaly detection models on edge devices using ArgoCD.
5. Introduction to Apache Kafka and S3
- Explore Apache Kafka for real-time data streaming and Amazon S3 for scalable storage solutions.
6. Viewing Kafka Messages in the Data Lake
- Learn how to view and analyze Kafka messages stored in a data lake for better insights.
7. What is Prometheus?
- Get to know Prometheus, an open-source monitoring and alerting toolkit, and its application in monitoring edge devices.
8. Monitoring Application Metrics with Prometheus
- Detailed instructions on setting up Prometheus to monitor the performance and health of your anomaly detection system.
9. What is Camel K?
- Introduction to Camel K, a lightweight integration framework built on Apache Camel, designed for Kubernetes.
10. Configuring Camel K Integrations for Data Pipelines
- Learn how to configure Camel K for seamless data pipeline integrations in your anomaly detection workflow.
11. What is a Jupyter Notebook?
- Overview of Jupyter Notebooks, an open-source web application for creating and sharing documents with live code, equations, visualizations, and narrative text.
12. Jupyter Notebooks with Code Examples
- Hands-on examples and code snippets in Jupyter Notebooks to help you implement and test anomaly detection models.
Choosing The Best AWS Service For Your Website + API.pptx
Have you ever been confused by the myriad of choices offered by AWS for hosting a website or an API?
Lambda, Elastic Beanstalk, Lightsail, Amplify, S3 (and more!) can each host websites + APIs. But which one should we choose?
Which one is cheapest? Which one is fastest? Which one will scale to meet our needs?
Join me in this session as we dive into each AWS hosting service to determine which one is best for your scenario and explain why!
20240605 QFM017 Machine Intelligence Reading List May 2024
Everything I found interesting about machines behaving intelligently during May 2024
Driving Business Innovation: Latest Generative AI Advancements & Success Story
Are you ready to revolutionize how you handle data? Join us for a webinar where we’ll bring you up to speed with the latest advancements in Generative AI technology and discover how leveraging FME with tools from giants like Google Gemini, Amazon, and Microsoft OpenAI can supercharge your workflow efficiency.
During the hour, we’ll take you through:
Guest Speaker Segment with Hannah Barrington: Dive into the world of dynamic real estate marketing with Hannah, the Marketing Manager at Workspace Group. Hear firsthand how their team generates engaging descriptions for thousands of office units by integrating diverse data sources—from PDF floorplans to web pages—using FME transformers, like OpenAIVisionConnector and AnthropicVisionConnector. This use case will show you how GenAI can streamline content creation for marketing across the board.
Ollama Use Case: Learn how Scenario Specialist Dmitri Bagh has utilized Ollama within FME to input data, create custom models, and enhance security protocols. This segment will include demos to illustrate the full capabilities of FME in AI-driven processes.
Custom AI Models: Discover how to leverage FME to build personalized AI models using your data. Whether it’s populating a model with local data for added security or integrating public AI tools, find out how FME facilitates a versatile and secure approach to AI.
We’ll wrap up with a live Q&A session where you can engage with our experts on your specific use cases, and learn more about optimizing your data workflows with AI.
This webinar is ideal for professionals seeking to harness the power of AI within their data management systems while ensuring high levels of customization and security. Whether you're a novice or an expert, gain actionable insights and strategies to elevate your data processes. Join us to see how FME and AI can revolutionize how you work with data!
HCL Notes und Domino Lizenzkostenreduzierung in der Welt von DLAU
Webinar Recording: https://www.panagenda.com/webinars/hcl-notes-und-domino-lizenzkostenreduzierung-in-der-welt-von-dlau/
DLAU und die Lizenzen nach dem CCB- und CCX-Modell sind für viele in der HCL-Community seit letztem Jahr ein heißes Thema. Als Notes- oder Domino-Kunde haben Sie vielleicht mit unerwartet hohen Benutzerzahlen und Lizenzgebühren zu kämpfen. Sie fragen sich vielleicht, wie diese neue Art der Lizenzierung funktioniert und welchen Nutzen sie Ihnen bringt. Vor allem wollen Sie sicherlich Ihr Budget einhalten und Kosten sparen, wo immer möglich. Das verstehen wir und wir möchten Ihnen dabei helfen!
Wir erklären Ihnen, wie Sie häufige Konfigurationsprobleme lösen können, die dazu führen können, dass mehr Benutzer gezählt werden als nötig, und wie Sie überflüssige oder ungenutzte Konten identifizieren und entfernen können, um Geld zu sparen. Es gibt auch einige Ansätze, die zu unnötigen Ausgaben führen können, z. B. wenn ein Personendokument anstelle eines Mail-Ins für geteilte Mailboxen verwendet wird. Wir zeigen Ihnen solche Fälle und deren Lösungen. Und natürlich erklären wir Ihnen das neue Lizenzmodell.
Nehmen Sie an diesem Webinar teil, bei dem HCL-Ambassador Marc Thomas und Gastredner Franz Walder Ihnen diese neue Welt näherbringen. Es vermittelt Ihnen die Tools und das Know-how, um den Überblick zu bewahren. Sie werden in der Lage sein, Ihre Kosten durch eine optimierte Domino-Konfiguration zu reduzieren und auch in Zukunft gering zu halten.
Diese Themen werden behandelt
- Reduzierung der Lizenzkosten durch Auffinden und Beheben von Fehlkonfigurationen und überflüssigen Konten
- Wie funktionieren CCB- und CCX-Lizenzen wirklich?
- Verstehen des DLAU-Tools und wie man es am besten nutzt
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- Praxisbeispiele und Best Practices zum sofortigen Umsetzen
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みなさんこんにちはこれ何文字まで入るの？40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの？えこ...
みなさんこんにちはこれ何文字まで入るの？40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの？えこ...
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CAKE: Sharing Slices of Confidential Data on Blockchain
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“Building and Scaling AI Applications with the Nx AI Manager,” a Presentation...
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GraphRAG for Life Science to increase LLM accuracy
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Monitoring and Managing Anomaly Detection on OpenShift.pdf
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Choosing The Best AWS Service For Your Website + API.pptx
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20240605 QFM017 Machine Intelligence Reading List May 2024
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Driving Business Innovation: Latest Generative AI Advancements & Success Story
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HCL Notes und Domino Lizenzkostenreduzierung in der Welt von DLAU
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Rocket Launcher Mechanism of Collaborative Actin Assembly Defined by Single-Molecule Imaging
- 1. Cytoskeleton Journal Club June 13, 2012 Presented by Suk Namgoong
- 2. Adenomanous Polypolis Coli (APC) • Main component of Wnt Signaling Pathway • In the absence of bounded Wnt on Wnt receptor, it forms ‘destruction complex’ for β-catanin, - mediates proteolytic degradation of β-catenin • By the bound of wnt into wnt receptor Frizzled, APC release β-catenin and β- catenin was translocated into nucleus and ctivated various Wnt target genes (Development, Morphogenesis, Cancers..) • Tumor Suppressor Gene - Mutations on APC gene cause a hereditary cancer syndrome called Familiar Adenomanous Polypolis (FAP) - Loss of APC functions by mutations has been found frequently colorectal cancers
- 3. APC directly nucleates actin assembly via C-terminal Basic Domain © 2010 Okada et al. Okada K et al. J Cell Biol 2010;189:1087-1096
- 4. No Sequence Homology with known actin binding domain/motifs It is dimer.
- 5. mDia1 and APC-B synergize to promote actin assembly in the combined presence of profilin and capping protein. Okada K et al. J Cell Biol 2010;189:1087-1096 © 2010 Okada et al.
- 6. Two different binding mode of Actin nucleators Arp2/3 complex stay on the ‘pointed end’ (slow growing end) of actin filaments Formins remains processively bound on ‘barbed end’ (fast growing end) How about APC? From which side of actin filaments it initiate actin nucleation?
- 7. Synergistic actin nucleations by APC and mDia1 (Cappuccino) Yeast Bnr1 – bud6 During the nucleation, Formins and other nucleation factors interact each other and synergize nucleations. But what will happen after nucleation? Do formins and other nucleators dissociate after nucleation? or formins carry all other stuffs along with elongation? (not likely, but we don‟t know yet)
- 8. 54 9 SNAP FH1 FH2 64 APC (Basic) SNAP 7 Oregon-Green Labeled Actin 64 7 PEG A Laser excitation
- 12. Complex Formations of mDia1, APC-C, OregonGreen-Actin (LatB treated)
- 17. Namgoong et al., Nature Struct Mol. Biol. 2011 I feel De ja vu…..
- 18. APC VopL APC stays bound on the pointed end of actin Filaments VopL is dissociated from the filament end and „spit out‟ new filaments continuously vs “Rocket Launcher” “Machine Gun”