Okazaki fragments are short, newly synthesized DNA fragments formed on the lagging template strand during DNA replication. They are between 1000-2000 nucleotides long in E. coli and approximately 150 nucleotides long in eukaryotes, separated by RNA primers. The RNA primers are removed and DNA ligase connects the Okazaki fragments into a continuous complementary strand.

1. Ahmed Ghobashi
Okazaki fragments

2.  Okazaki fragments are short, newly synthesized
DNA fragments that are formed on the lagging
template strand during DNA replication.
 Okazaki fragments are between 1000 and 2000
nucleotides long in Escherichia coli and are
approximately 150 nucleotides long in eukaryotes.
 They are separated by ~10-nucleotide RNA primers
and are unligated until RNA primers are removed,
followed by enzyme ligase connecting the two
Okazaki fragments into one continuous newly
synthesized complementary strand.

3.  On the leading strand DNA replication proceeds
continuously along the DNA molecule, but on the
lagging strand the new DNA is made in fragments,
which are later joined together by a DNA ligase
enzyme.
 This is because the enzymes that synthesise the
new DNA can only work in one direction along the
parent DNA molecule (DNA is synthesized from 5' to
3).