After a pilot scale production of a rhamnolipidic biosurfactant, it is purified using two methods, chemical separation and chromatography. We use UPLC-MS/MS for the characterization of the chemical species.
This document summarizes research on using commercial chiral anion-exchange LC columns packed with quinidine or quinine ligands to separate enantiomers of acidic drugs and related compounds using hydro-organic mobile phases. Key findings include:
1) Low pH mobile phases provided the best retention and enantioresolution. Selectivity was largely independent of mobile phase variables except at pH >5-6.
2) Enantioseparation was achieved for a range of drug acids including NSAIDs and mandelic acids. However, enantioselectivity was not sufficient to explore achiral-chiral separations in a single column.
3) Retention was very similar across the three columns tested,
This study used Taguchi methods to determine optimal conditions for treating tannery wastewater through coagulation-flocculation. Jar tests were conducted to evaluate the effects of coagulant type and dose, coagulant aid type and dose, and pH on removal of chemical oxygen demand (COD), chromium, total dissolved solids (TDS), and turbidity. The optimal conditions found were 800 ppm FeCl3 coagulant with 600 ppm Na2CO3 aid at pH 7.5 for COD removal, 1600 ppm FeCl3 with 100 ppm CaO aid at pH 7.5 for chromium removal, 1600 ppm FeCl3 at pH 9 with 300 ppm Na2SiO3 aid
This document describes a study that developed and optimized an ion-pair reverse-phase high-performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (HPLC-ICP-MS) method for arsenic speciation in Chinese brake fern (Pteris vittata L.). The method was able to separate and detect four arsenic species (arsenite [As(III)], dimethyl arsenic acid (DMA), monomethyl arsenic (MMA), and arsenate [As(V)]) in a single run. This is the first study to show that Chinese brake fern can convert MMA to DMA via methylation. The optimized HPLC-ICP-MS method was then applied to
1) The critical micelle concentration (CMC) of sodium dodecyl sulphate (SDS) was studied at various pH levels between 2 and 10 using dye absorption, conductance, and light scattering measurement techniques.
2) The results showed that at low pH (below pH 4), the CMC decreases, whereas at higher pH (between 5 and 10) it remains constant.
3) This suggests that pH has an effect on the CMC of SDS, likely by decreasing the electrostatic repulsion of the charge heads on the micelle surface and changing the stability of the micelle at low pH levels.
1. The document describes a method to determine the Rf value of Betaxolol drugs using thin layer chromatography (TLC) with a ligand exchange method.
2. L-leucine was used as the chiral ligand exchange reagent to resolve the enantiomers of Betaxolol. TLC plates were prepared with silica gel and impregnated with the LER.
3. Various solvent systems were tested as mobile phases on the TLC plates. The successful mobile phases and calculated Rf values are reported, with Rf values ranging from 0.5652 to 0.6451.
This short presentation describes the principles of measuring lipophilicty and bio-mimetic properties, such as protein and phospholipid binding by HPLC. These data can be used to model compound's in vivo distribution.
Compositional analysis of Polysaccharide Arunima Sur
This document discusses various methods for compositional analysis of polysaccharides, including enzymatic treatment, hydrolysis, and analytical techniques like chromatography and NMR spectroscopy. It provides details on enzymatic treatment to remove proteins and lipids before analysis. Hydrolysis and specific endoglycosidases are used to degrade polysaccharides into smaller units. Gas chromatography, high performance liquid chromatography, and NMR spectroscopy are then used to separate and identify the monosaccharide units. The document outlines the instrumentation and principles of these analytical methods.
This document summarizes research on using commercial chiral anion-exchange LC columns packed with quinidine or quinine ligands to separate enantiomers of acidic drugs and related compounds using hydro-organic mobile phases. Key findings include:
1) Low pH mobile phases provided the best retention and enantioresolution. Selectivity was largely independent of mobile phase variables except at pH >5-6.
2) Enantioseparation was achieved for a range of drug acids including NSAIDs and mandelic acids. However, enantioselectivity was not sufficient to explore achiral-chiral separations in a single column.
3) Retention was very similar across the three columns tested,
This study used Taguchi methods to determine optimal conditions for treating tannery wastewater through coagulation-flocculation. Jar tests were conducted to evaluate the effects of coagulant type and dose, coagulant aid type and dose, and pH on removal of chemical oxygen demand (COD), chromium, total dissolved solids (TDS), and turbidity. The optimal conditions found were 800 ppm FeCl3 coagulant with 600 ppm Na2CO3 aid at pH 7.5 for COD removal, 1600 ppm FeCl3 with 100 ppm CaO aid at pH 7.5 for chromium removal, 1600 ppm FeCl3 at pH 9 with 300 ppm Na2SiO3 aid
This document describes a study that developed and optimized an ion-pair reverse-phase high-performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (HPLC-ICP-MS) method for arsenic speciation in Chinese brake fern (Pteris vittata L.). The method was able to separate and detect four arsenic species (arsenite [As(III)], dimethyl arsenic acid (DMA), monomethyl arsenic (MMA), and arsenate [As(V)]) in a single run. This is the first study to show that Chinese brake fern can convert MMA to DMA via methylation. The optimized HPLC-ICP-MS method was then applied to
1) The critical micelle concentration (CMC) of sodium dodecyl sulphate (SDS) was studied at various pH levels between 2 and 10 using dye absorption, conductance, and light scattering measurement techniques.
2) The results showed that at low pH (below pH 4), the CMC decreases, whereas at higher pH (between 5 and 10) it remains constant.
3) This suggests that pH has an effect on the CMC of SDS, likely by decreasing the electrostatic repulsion of the charge heads on the micelle surface and changing the stability of the micelle at low pH levels.
1. The document describes a method to determine the Rf value of Betaxolol drugs using thin layer chromatography (TLC) with a ligand exchange method.
2. L-leucine was used as the chiral ligand exchange reagent to resolve the enantiomers of Betaxolol. TLC plates were prepared with silica gel and impregnated with the LER.
3. Various solvent systems were tested as mobile phases on the TLC plates. The successful mobile phases and calculated Rf values are reported, with Rf values ranging from 0.5652 to 0.6451.
This short presentation describes the principles of measuring lipophilicty and bio-mimetic properties, such as protein and phospholipid binding by HPLC. These data can be used to model compound's in vivo distribution.
Compositional analysis of Polysaccharide Arunima Sur
This document discusses various methods for compositional analysis of polysaccharides, including enzymatic treatment, hydrolysis, and analytical techniques like chromatography and NMR spectroscopy. It provides details on enzymatic treatment to remove proteins and lipids before analysis. Hydrolysis and specific endoglycosidases are used to degrade polysaccharides into smaller units. Gas chromatography, high performance liquid chromatography, and NMR spectroscopy are then used to separate and identify the monosaccharide units. The document outlines the instrumentation and principles of these analytical methods.
High performance liquid chromatography stationary phases can separate polar and nonpolar compounds using reversed-phase or normal phase columns. Aqueous normal phase chromatography uses silicon-hydride stationary phases to retain both polar and nonpolar analytes in a single isocratic run through dual retention mechanisms. This distinguishes it from other chromatographic methods that can only separate compounds of specific polarities.
This study investigated the polymerization of lactic acid as a model for prebiotic peptide formation via ester-amide exchange. Lactic acid was polymerized in a closed system at 85°C over various time points. HPLC and 1H-NMR were used to analyze the polymers and determine degree of polymerization (DP) and total lactic acid units. DP was found to increase with time while total units decreased, showing polymer regeneration. Methods showed consistent results within 10-15% error. Further studies will compare kinetics to a computer simulation to determine rate constants and model polymerization from various monomers.
Undergraduate Research Poster Louis TerryLouis Terry
This study examined the photodegradation of the pharmaceutical ranitidine when exposed to solar light in various concentrations of humic acid solutions. Ranitidine degraded more quickly with higher humic acid concentrations, with half-lives ranging from 95 minutes in pure water to 7 minutes in a 20 ppm humic acid solution. High performance liquid chromatography and mass spectrometry identified potential photodegradation products and helped determine degradation rates. Future work will further characterize degradation products through additional analysis.
Reversed-Flow Gas Chromatography (RF-GC) is a relatively new technique to determine the physicochemical properties of solute. So far, RF-GC has been used to determine the diffusion coefficient of various solvents. However, the used of RF-GC in other applications such as environmental studies have not been reported. In this study, RF-GC which is a part of flow-perturbation gas chromatography was used to investigate the rate of evaporation of methanol in the presence surfactant (Triton X-100). Waste methanol is considered as an ignitable hazardous waste by US Environmental Protection Agency (USEPA) when its concentrations is equal to or greater than 24% in water. The aim of this study was to determine the effect of Triton X-100 as non-ionic surfactant to suppress evaporation rate of methanol. The result of this study indicated that the evaporation rate of methanol was found to be retarded by the presence of Triton X-100. The percentage of retardation was found to increase with increasing concentration of Triton X-100.
This document discusses the role of dehydration catalyst acid properties on one-step DME synthesis using physical mixtures. It summarizes the results of experiments testing various solid acid catalysts for methanol dehydration and one-step DME synthesis from syngas. The main findings are:
1) Catalyst acidity, determined by pyridine adsorption IR spectroscopy, affected activity for methanol dehydration, with stronger acid sites correlating to higher rates.
2) In one-step DME synthesis, addition of an acid catalyst to the methanol synthesis catalyst strongly increased CO conversion.
3) The determining rate of direct DME synthesis appears to be controlled by the acid properties, specifically the strength
Up-flow anaerobic sludge beds (UASB) reactors utilize waste-converting biofilms to treat toxic wastewater. Microorganisms in the biofilm granules break down compounds anaerobically and produce methane gas. Chemical oxygen demand (COD) analysis measures the organic compounds in water and is used to determine the quality of treated effluent from UASB reactors, with the goal of reducing COD levels by 60-80%. Three UASB reactors were established in this study to collect samples from ports throughout and analyze COD levels over time, with initial results indicating the reactors are reducing COD as intended. However, further analysis is needed to fully understand the microbial interactions and optimize the reactors'
This document presents a kinetic method for determining microquantities of D(-)-arabinose. The method relies on D(-)-arabinose accelerating the reaction between molybdenum(VI) and hydrogen peroxide in a 50% acetonitrile solution. Optimal experimental conditions were determined by studying the kinetics of this reaction with and without D(-)-arabinose. D(-)-Arabinose concentrations from 46 to 135 μg/ml were determined photometrically by measuring the rate of colored product formation. The standard deviation was less than 10%. This kinetic method allows for the determination of microquantities of D(-)-arabinose.
1) The document discusses methods for determining soil calcium carbonate equivalent (CCE), including volumetric calcimetry and titrimetry.
2) Key parameters that laboratories provided for the volumetric calcimetry method include particle size and sample mass, type and volume of acid used, reaction time, and volume and composition of the calcimeter solution.
3) The document proposes a standardized method for calcimetry using a particle size of 100 mesh, sample mass of 0.5-5g, 4N HCl, 7mL acid, 20mL water, and a 10% NaCl calcimeter solution.
The most common method of preserving raw hidesis brine curing with sodium chloride. However, thisprocess has three important disadvantages: first, thelength of time that it takes, which is a minimum of18 hours; second, the insufficient degree of curingreached in some hides due to an overload andpossibly the low efficiency of the brine raceway; andfinally, the environmental impact associated withthe discharge of large quantities of electrolytes in thesoaking step. Our long term goal is to address allthree issues. Initially, we have carried out a studyof the salt uptake and its diffusion mechanism inorder to attempt a reduction in the curing time. Acontinuous reaction mathematical model of a closedone dimensional system that describes the diffusionof sodium chloride in the hide during the curingprocess was chosen in the search for the optimumbrine curing conditions such as the optimum brineconcentration and percent float. The effect of thesetwo parameters on the values of transport coefficient
was reported. Brine diffusion into the hide wastracked by measurement of the chloride concentrationof the residual brine solution. In addition, a piece ofhide was cured with a fluorescently labeled brinesolution and analyzed by means of epifluorescentmicroscopy for direct visualization of the sodiumlocation within the hide.
This journal article describes three methods for determining two mixtures containing drotaverine hydrochloride: 1) HPLC separation and UV detection, 2) partial least squares chemometrics assisted spectrophotometry, and 3) principal component regression chemometrics assisted spectrophotometry. These methods were validated using internal validation, crossvalidation, and external validation on laboratory mixtures and pharmaceutical preparations. The results of the chemometrics methods agreed well with the HPLC method.
Water Analysis Using LAMBDA UV/Visible Spectrophotometers: Ammonia-Nitrogen D...PerkinElmer, Inc.
Ammonia-nitrogen (NH3-N) occurs
naturally in groundwaters at concentrations below 0.2 mg/L and up to 12 mg/L in surface waters, as a result of decomposition of organic matter. High concentrations of ammonia in surface waters are toxic to aquatic life
and are indicative of contamination from industrial effluent, raw sewage and agricultural runoff. Ultimately the pH value of the water determines whether ammonia-nitrogen is found as NH4+, NH4OH or NH3.
In this application, the quantitative analysis of ammonia-nitrogen was performed using the LAMBDA 265™ UV/Vis spectrophotometer and CHEMetrics ammonia nitrogen cell test.
If you want to know more, please visit https://www.creative-proteomics.com/services/short-chain-fatty-acids-analysis-service.htm. Short chain fatty acids (SCFAs) are defined as fatty acids with two to six carbon atoms. SCFAs have a wide range of metabolic effects. And SCFA profiling has been a major topic in gut bacteria studies.
The document reports on an experimental study to determine the pKa (acid dissociation constant) of perfluorooctanoic acid (PFOA) using potentiometric titration in a water-methanol mixed solvent system. The study found:
1) The pKa of monomeric PFOA was determined to be 3.8 ± 0.1. This value is important for understanding the environmental fate of PFOA as both its ionized and non-ionized forms have different physicochemical properties.
2) The pKa was suppressed to around 2.3 at higher PFOA concentrations due to aggregation of its conjugate base. Many previous studies measured partitioning coefficients at concentrations above environmental levels,
Water Analysis Using LAMBDA UV/Visible Spectrophotometers: Formaldehyde Deter...PerkinElmer, Inc.
In this application, the quantitative analysis of formaldehyde was successfully executed using the LAMBDA™ 265 UV/Vis spectrophotometer and Merck Spectroquant® formaldehyde cell test kit. Principle Formaldehyde reacts with chromotropic acid in sulphuric solution to form a violet dye which can be detected photometrically at 565 nm. The formaldehyde cell test kit is appropriate for the concentration range of 0.10 to 8.00 mg/L formaldehyde allowing the concentration of formaldehyde in a water sample to be determined without the use of a calibration curve by multiplying the measured absorbance at 565 nm by a known factor.
IOSR Journal of Applied Physics (IOSR-JAP) is an open access international journal that provides rapid publication (within a month) of articles in all areas of physics and its applications. The journal welcomes publications of high quality papers on theoretical developments and practical applications in applied physics. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Water Analysis Using LAMBDA UV/Visible Spectrophotometers Hexavalent Chromium...PerkinElmer, Inc.
This document describes a method for determining hexavalent chromium concentration in water samples using a UV/Vis spectrophotometer and chromate cell test. Chromium (VI) ions react with diphenylcarbazide to form a red-violet complex, which is detected photometrically at 550 nm. Measurement of standard solutions showed high accuracy and repeatability, with concentrations calculated directly from absorbance readings using a known factor. The method provides a rapid and simple way to quantify chromium (VI) in water samples.
ElogPoct: A Tool for Lipophilicity Determination in Drug DiscoveryBrian Bissett
This document presents a new RP-HPLC method for determining logPoct values, a measure of lipophilicity important for drug discovery. The key advantages of the method are that it is rapid (determinations in 20 minutes on average), uses small amounts of compound (1 mL of a 30-50 μg/mL solution), has a wide dynamic range of 6 log units, and shows good accuracy and reproducibility when tested on 36 drug molecules. A comparison to experimentally determined logPoct values indicates the new RP-HPLC method performs comparably to traditional shake-flask methods.
The document discusses applications of liquid chromatography-mass spectrometry (LCMS). It describes how LCMS combines liquid chromatography separation with mass spectrometry detection. Several studies using LCMS are summarized, including analyzing phytochemicals in Solanum plants and identifying compounds in Archidendron bubalinum seed shell extracts. Key components detected include chlorogenic acids, flavonoids, alkaloids, and the compounds phlorizin and astilbin.
This document summarizes a study that analyzed the kinetics of proton transport by the M2 protein channel from influenza A virus. The researchers found that proton flux through M2 follows Michaelis-Menten kinetics with two saturation steps around pH 5.5 and pH 4. They determined rate constants and apparent pKa values that matched the pKa values of the histidine residues in M2 that were previously observed. Mutagenesis studies identified residues important for proton entrance, relay, and exit rates. NMR experiments showed synchronized conformational changes between the histidine and tryptophan gate residues during activation.
Residuos de flores como adsorventes de bajo costoEmiy Nicole
This document describes research on using flower waste (carnation, rose, and daisy stalks) as a low-cost adsorbent to remove Acid Blue 9 dye from water. Batch experiments tested the effect of pH, adsorbent dosage, and initial dye concentration on dye removal efficiency. Equilibrium was modeled using the Langmuir and Freundlich isotherms, with better fit to the Freundlich model. Kinetic studies fit best to a pseudo-second order model. Over 90% removal was achieved at pH 2.0 using 15 mg/L initial dye concentration and 4 g/L adsorbent dosage. The flower wastes were characterized and showed potential as an alternative,
TLC Separation of Cephalosporins on Stannic Arsenate LayersIOSR Journals
Abstract: The chromatographic behaviour of some cephalosporins has been studied on synthetic stannic arsenate layers using citrate and borate buffers as mobile phases. Several ternary and quaternary separations have been achieved. The utility of these separations has been demonstrated for estimation of cephalosporins in blood serum from patients.This method used is simple, rapid ,reproducible and can also be applied in the separation and determination of cephalosporins in other biological samples. The limit of detection was found to be 0.20 μg/l.
High performance liquid chromatography stationary phases can separate polar and nonpolar compounds using reversed-phase or normal phase columns. Aqueous normal phase chromatography uses silicon-hydride stationary phases to retain both polar and nonpolar analytes in a single isocratic run through dual retention mechanisms. This distinguishes it from other chromatographic methods that can only separate compounds of specific polarities.
This study investigated the polymerization of lactic acid as a model for prebiotic peptide formation via ester-amide exchange. Lactic acid was polymerized in a closed system at 85°C over various time points. HPLC and 1H-NMR were used to analyze the polymers and determine degree of polymerization (DP) and total lactic acid units. DP was found to increase with time while total units decreased, showing polymer regeneration. Methods showed consistent results within 10-15% error. Further studies will compare kinetics to a computer simulation to determine rate constants and model polymerization from various monomers.
Undergraduate Research Poster Louis TerryLouis Terry
This study examined the photodegradation of the pharmaceutical ranitidine when exposed to solar light in various concentrations of humic acid solutions. Ranitidine degraded more quickly with higher humic acid concentrations, with half-lives ranging from 95 minutes in pure water to 7 minutes in a 20 ppm humic acid solution. High performance liquid chromatography and mass spectrometry identified potential photodegradation products and helped determine degradation rates. Future work will further characterize degradation products through additional analysis.
Reversed-Flow Gas Chromatography (RF-GC) is a relatively new technique to determine the physicochemical properties of solute. So far, RF-GC has been used to determine the diffusion coefficient of various solvents. However, the used of RF-GC in other applications such as environmental studies have not been reported. In this study, RF-GC which is a part of flow-perturbation gas chromatography was used to investigate the rate of evaporation of methanol in the presence surfactant (Triton X-100). Waste methanol is considered as an ignitable hazardous waste by US Environmental Protection Agency (USEPA) when its concentrations is equal to or greater than 24% in water. The aim of this study was to determine the effect of Triton X-100 as non-ionic surfactant to suppress evaporation rate of methanol. The result of this study indicated that the evaporation rate of methanol was found to be retarded by the presence of Triton X-100. The percentage of retardation was found to increase with increasing concentration of Triton X-100.
This document discusses the role of dehydration catalyst acid properties on one-step DME synthesis using physical mixtures. It summarizes the results of experiments testing various solid acid catalysts for methanol dehydration and one-step DME synthesis from syngas. The main findings are:
1) Catalyst acidity, determined by pyridine adsorption IR spectroscopy, affected activity for methanol dehydration, with stronger acid sites correlating to higher rates.
2) In one-step DME synthesis, addition of an acid catalyst to the methanol synthesis catalyst strongly increased CO conversion.
3) The determining rate of direct DME synthesis appears to be controlled by the acid properties, specifically the strength
Up-flow anaerobic sludge beds (UASB) reactors utilize waste-converting biofilms to treat toxic wastewater. Microorganisms in the biofilm granules break down compounds anaerobically and produce methane gas. Chemical oxygen demand (COD) analysis measures the organic compounds in water and is used to determine the quality of treated effluent from UASB reactors, with the goal of reducing COD levels by 60-80%. Three UASB reactors were established in this study to collect samples from ports throughout and analyze COD levels over time, with initial results indicating the reactors are reducing COD as intended. However, further analysis is needed to fully understand the microbial interactions and optimize the reactors'
This document presents a kinetic method for determining microquantities of D(-)-arabinose. The method relies on D(-)-arabinose accelerating the reaction between molybdenum(VI) and hydrogen peroxide in a 50% acetonitrile solution. Optimal experimental conditions were determined by studying the kinetics of this reaction with and without D(-)-arabinose. D(-)-Arabinose concentrations from 46 to 135 μg/ml were determined photometrically by measuring the rate of colored product formation. The standard deviation was less than 10%. This kinetic method allows for the determination of microquantities of D(-)-arabinose.
1) The document discusses methods for determining soil calcium carbonate equivalent (CCE), including volumetric calcimetry and titrimetry.
2) Key parameters that laboratories provided for the volumetric calcimetry method include particle size and sample mass, type and volume of acid used, reaction time, and volume and composition of the calcimeter solution.
3) The document proposes a standardized method for calcimetry using a particle size of 100 mesh, sample mass of 0.5-5g, 4N HCl, 7mL acid, 20mL water, and a 10% NaCl calcimeter solution.
The most common method of preserving raw hidesis brine curing with sodium chloride. However, thisprocess has three important disadvantages: first, thelength of time that it takes, which is a minimum of18 hours; second, the insufficient degree of curingreached in some hides due to an overload andpossibly the low efficiency of the brine raceway; andfinally, the environmental impact associated withthe discharge of large quantities of electrolytes in thesoaking step. Our long term goal is to address allthree issues. Initially, we have carried out a studyof the salt uptake and its diffusion mechanism inorder to attempt a reduction in the curing time. Acontinuous reaction mathematical model of a closedone dimensional system that describes the diffusionof sodium chloride in the hide during the curingprocess was chosen in the search for the optimumbrine curing conditions such as the optimum brineconcentration and percent float. The effect of thesetwo parameters on the values of transport coefficient
was reported. Brine diffusion into the hide wastracked by measurement of the chloride concentrationof the residual brine solution. In addition, a piece ofhide was cured with a fluorescently labeled brinesolution and analyzed by means of epifluorescentmicroscopy for direct visualization of the sodiumlocation within the hide.
This journal article describes three methods for determining two mixtures containing drotaverine hydrochloride: 1) HPLC separation and UV detection, 2) partial least squares chemometrics assisted spectrophotometry, and 3) principal component regression chemometrics assisted spectrophotometry. These methods were validated using internal validation, crossvalidation, and external validation on laboratory mixtures and pharmaceutical preparations. The results of the chemometrics methods agreed well with the HPLC method.
Water Analysis Using LAMBDA UV/Visible Spectrophotometers: Ammonia-Nitrogen D...PerkinElmer, Inc.
Ammonia-nitrogen (NH3-N) occurs
naturally in groundwaters at concentrations below 0.2 mg/L and up to 12 mg/L in surface waters, as a result of decomposition of organic matter. High concentrations of ammonia in surface waters are toxic to aquatic life
and are indicative of contamination from industrial effluent, raw sewage and agricultural runoff. Ultimately the pH value of the water determines whether ammonia-nitrogen is found as NH4+, NH4OH or NH3.
In this application, the quantitative analysis of ammonia-nitrogen was performed using the LAMBDA 265™ UV/Vis spectrophotometer and CHEMetrics ammonia nitrogen cell test.
If you want to know more, please visit https://www.creative-proteomics.com/services/short-chain-fatty-acids-analysis-service.htm. Short chain fatty acids (SCFAs) are defined as fatty acids with two to six carbon atoms. SCFAs have a wide range of metabolic effects. And SCFA profiling has been a major topic in gut bacteria studies.
The document reports on an experimental study to determine the pKa (acid dissociation constant) of perfluorooctanoic acid (PFOA) using potentiometric titration in a water-methanol mixed solvent system. The study found:
1) The pKa of monomeric PFOA was determined to be 3.8 ± 0.1. This value is important for understanding the environmental fate of PFOA as both its ionized and non-ionized forms have different physicochemical properties.
2) The pKa was suppressed to around 2.3 at higher PFOA concentrations due to aggregation of its conjugate base. Many previous studies measured partitioning coefficients at concentrations above environmental levels,
Water Analysis Using LAMBDA UV/Visible Spectrophotometers: Formaldehyde Deter...PerkinElmer, Inc.
In this application, the quantitative analysis of formaldehyde was successfully executed using the LAMBDA™ 265 UV/Vis spectrophotometer and Merck Spectroquant® formaldehyde cell test kit. Principle Formaldehyde reacts with chromotropic acid in sulphuric solution to form a violet dye which can be detected photometrically at 565 nm. The formaldehyde cell test kit is appropriate for the concentration range of 0.10 to 8.00 mg/L formaldehyde allowing the concentration of formaldehyde in a water sample to be determined without the use of a calibration curve by multiplying the measured absorbance at 565 nm by a known factor.
IOSR Journal of Applied Physics (IOSR-JAP) is an open access international journal that provides rapid publication (within a month) of articles in all areas of physics and its applications. The journal welcomes publications of high quality papers on theoretical developments and practical applications in applied physics. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Water Analysis Using LAMBDA UV/Visible Spectrophotometers Hexavalent Chromium...PerkinElmer, Inc.
This document describes a method for determining hexavalent chromium concentration in water samples using a UV/Vis spectrophotometer and chromate cell test. Chromium (VI) ions react with diphenylcarbazide to form a red-violet complex, which is detected photometrically at 550 nm. Measurement of standard solutions showed high accuracy and repeatability, with concentrations calculated directly from absorbance readings using a known factor. The method provides a rapid and simple way to quantify chromium (VI) in water samples.
ElogPoct: A Tool for Lipophilicity Determination in Drug DiscoveryBrian Bissett
This document presents a new RP-HPLC method for determining logPoct values, a measure of lipophilicity important for drug discovery. The key advantages of the method are that it is rapid (determinations in 20 minutes on average), uses small amounts of compound (1 mL of a 30-50 μg/mL solution), has a wide dynamic range of 6 log units, and shows good accuracy and reproducibility when tested on 36 drug molecules. A comparison to experimentally determined logPoct values indicates the new RP-HPLC method performs comparably to traditional shake-flask methods.
The document discusses applications of liquid chromatography-mass spectrometry (LCMS). It describes how LCMS combines liquid chromatography separation with mass spectrometry detection. Several studies using LCMS are summarized, including analyzing phytochemicals in Solanum plants and identifying compounds in Archidendron bubalinum seed shell extracts. Key components detected include chlorogenic acids, flavonoids, alkaloids, and the compounds phlorizin and astilbin.
This document summarizes a study that analyzed the kinetics of proton transport by the M2 protein channel from influenza A virus. The researchers found that proton flux through M2 follows Michaelis-Menten kinetics with two saturation steps around pH 5.5 and pH 4. They determined rate constants and apparent pKa values that matched the pKa values of the histidine residues in M2 that were previously observed. Mutagenesis studies identified residues important for proton entrance, relay, and exit rates. NMR experiments showed synchronized conformational changes between the histidine and tryptophan gate residues during activation.
Residuos de flores como adsorventes de bajo costoEmiy Nicole
This document describes research on using flower waste (carnation, rose, and daisy stalks) as a low-cost adsorbent to remove Acid Blue 9 dye from water. Batch experiments tested the effect of pH, adsorbent dosage, and initial dye concentration on dye removal efficiency. Equilibrium was modeled using the Langmuir and Freundlich isotherms, with better fit to the Freundlich model. Kinetic studies fit best to a pseudo-second order model. Over 90% removal was achieved at pH 2.0 using 15 mg/L initial dye concentration and 4 g/L adsorbent dosage. The flower wastes were characterized and showed potential as an alternative,
TLC Separation of Cephalosporins on Stannic Arsenate LayersIOSR Journals
Abstract: The chromatographic behaviour of some cephalosporins has been studied on synthetic stannic arsenate layers using citrate and borate buffers as mobile phases. Several ternary and quaternary separations have been achieved. The utility of these separations has been demonstrated for estimation of cephalosporins in blood serum from patients.This method used is simple, rapid ,reproducible and can also be applied in the separation and determination of cephalosporins in other biological samples. The limit of detection was found to be 0.20 μg/l.
Synthesis, Characterization and Antibacterial Activity of New Complexes of So...IOSR Journals
Complexes of some lanthanide picrates (Ln3+ = Pr3+, Nd3+ and Dy3+) with benzo-18-crown-6 and 221-cryptand were synthesized and characterized by elemental analysis, FTIR, and UV-Visible. Spectrophotometric methods, thermal analysis (TGA & DTG), melting point, magnetic susceptibility and molar conductance. Also an in-vitro study on gram positive (Staphylococcus aureus) and gram negative bacteria (Escherichia coli, Salmonella and pseudomonas aeruginosa) was performed and the results were compared to those of the broad spectrum antibiotic Chloramphinicol. The benzo-18-crown-6 complexes have the general formula of [Ln.L.(Pic)2]Pic.nH2O , where; (Ln3+ = Pr3+, Nd3+, and Dy3+) , (L = Benzo-18-crown-6) , (Pic = Picrate anion) , (n = 1-2). In these complexes two picrate anions are coordinated to the metal ion through the phenolic oxygen and oxygen of the ortho nitro group, thus, the metal ions in these complexes have a coordination number of (10). The complexes of 221-cryptand have the general formula of [Ln.L.(Pic)]Pic2.nH2O where; (Ln3+ = Pr3+, Nd3+, and Dy3+), (L = 221-cryptand), (Pic = Picrate anion), (n = 1,2 or 7). In these complexes one picrate anion is coordinated to the metal ion, also through the phenolic oxygen and the oxygen from the ortho nitro group, thus the metal ions in the cryptand complexes have a coordination number of (9).
In view of the medical and social problem caused by the increasing number of drug addicts the determination of opium alka-loids is of special importance. Morphine is known as a highly addictive and potent narcotic but drug users prefer heroin because of its more intense immediate effect. As heroin is hydrolysed in the organism to morphine the knowledge of the morphine content of biological fluids and tissues is indispensable for forensic and therapeutic purposes. The methods recently used for the determination of morphine in urine are gas-liquid chromatography 1, high-pressure liquid chromatography with fluorimetric determination 2 and gas chromatography mass spectrometry 3. In this paper a simple and inexpensive kinetic method is presented , based on the decomposition of the coloured compound formed by the reaction of hydrogen peroxide with cobalt(II) and morphine, in the presence of carbonate buffer. There is a definite concentration range over which the decomposition rate of the compound mentioned is a linear function of the morphine concentration.
Microchimica Acta Volume 84 issue 5-6 1984 [doi 10.1007_bf01197162] G. A. Mil...Sekheta Bros Company
This document describes a kinetic method for determining morphine concentration in urine samples. The method involves reacting morphine in the sample with hydrogen peroxide and cobalt(II) ions to form a colored compound. The rate of decomposition of this compound is measured photometrically and is directly proportional to the concentration of morphine over a certain range. The method was found to accurately determine morphine concentrations from 1.5 to 12.3 μg/ml in urine samples. It was also applied to analyze urine samples from individuals suspected of taking morphine or heroin and the results correlated well with an established HPLC method.
his study investigated the microbial community in a full scale anaerobic baffled reactor and sequencing batch reactor system for oil-produced water treatment in summer and winter. The community structures of fungi and bacteria were analyzed through polymerase chain reaction–denaturing gradient gel electrophoresis and Illumina high-throughput sequencing, respectively. Chemical oxygen demand effluent concentration achieved lower than 50 mg/L level after the system in both summer and winter, however, chemical oxygen demand removal rates after anaerobic baffled reactor treatment system were significant higher in summer than that in winter, which conformed to the microbial community diversity. Saccharomycotina, Fusarium, and Aspergillus were detected in both anaerobic baffled reactor and sequencing batch reactor during summer and winter. The fungal communities in anaerobic baffled reactor and sequencing batch reactor were shaped by seasons and treatment units, while there was no correlation between abundance of fungi and chemical oxygen demand removal rates. Compared to summer, the total amount of the dominant hydrocarbon degrading bacteria decreased by 10.2% in anaerobic baffled reactor, resulting in only around 23% of chemical oxygen demand was removed in winter. Although microbial community significantly varied in the three parallel sulfide reducing bacteria, the performance of these bioreactors had no significant difference between summer and winter.
International Journal of Engineering Research and Applications (IJERA) is an open access online peer reviewed international journal that publishes research and review articles in the fields of Computer Science, Neural Networks, Electrical Engineering, Software Engineering, Information Technology, Mechanical Engineering, Chemical Engineering, Plastic Engineering, Food Technology, Textile Engineering, Nano Technology & science, Power Electronics, Electronics & Communication Engineering, Computational mathematics, Image processing, Civil Engineering, Structural Engineering, Environmental Engineering, VLSI Testing & Low Power VLSI Design etc.
A HPLC-UV METHOD FOR DETEERMINATION OF THREE PESTICIDES IN WATERijac123
HPLC method is developed and validated for determination of three pesticides (abamectin, imidacloprid,
and β-cyfluthrin) in water. These pesticides are used widely in agriculture for crops protection, and may be
leached to the groundwater. Reversed-phase method with C18 column (5 µm, 250mm × 4.6 mm inner
diameter) using a mobile phase consisting of acetonitrile/water (v:v = 4:1) at a flow rate of 1.5 mL/min and
UV detection at 220 nm was used. This method is validated according to new methods which include
accuracy, precision, linearity and range, limit of detection and limit of quantitation. The current method
exhibits good linearity over the range of 1-1000 ppb for abamectin, 0.5-1000 ppb for imidacloprid, and
0.4-1000 ppb for ß-cyfluthrin with r2
greater than 0.990. The percentage recovery of the method at three
concentration levels (5, 100, and 1000 ppb) is within 97.6 to 101.5% for the three pesticides. Relative
standard deviation of the area of six replicate injections of each pesticide at three concentration levels (5.0,
100.0, and 1000.0 ppb) was found to be less than 1% which reflect the precision of the method. Limit of
quantitation of the three pesticides using this method is low (1.0, 0.5, and 0.4 ppb) for abamectin,
imidacloprid, and β-cyfluthrin, respectively which enables the determination of these three pesticides in
water at low concentration levels
STUDIES ON TREATMENT OF PHARMACEUTICAL WASTE EFFLUENTS BY POLYMER MATERIALS M...EDITOR IJCRCPS
In the present study, sorption technique was used to achieve the optimum recovery of the pharmaceutical waste from effluents.
The modified urea formaldehyde resin was prepared and mixed with inorganic adsorbent at various proportions. The removal
capabilities of pharmaceutical waste by the prepared composite materials were investigated. Different factors affecting the uptake
such as contact time, pH value and aqueous volume to resin weight ratio have been investigated. The reaction mechanisms and
the optimum conditions for the treatment were deduced in the light of the obtained results.
Keywords: Sorption technique, Pharmaceutical waste, Different factors.
This document summarizes the development of efficient protocols for synthesizing 1,2,3,4-tetrahydroisoquinolin-1-ones. Several methods were developed, including the use of Mitsunobu reactions, copper-catalyzed arylations, and SNAr reactions to install various substituents on the core scaffold. These methods proved to be versatile, efficient, and amenable to parallel synthesis, allowing for SAR exploration across different regions of the molecule.
This document provides an overview of chromatography. It begins with an introduction that defines chromatography and describes how it separates mixtures based on differences in solubility between components in mobile and stationary phases. The document then covers the history of chromatography, important technical terms, main types categorized by interaction with the stationary phase or physical state of the mobile phase. Applications are discussed in areas like drug development, food testing, and forensics. Advantages are noted as versatility in separation. Disadvantages include temperature sensitivity and ensuring solubility. References are listed at the end.
2022 - Removal of Cr(VI) from solution using UiO-66-NH2 prepared in a green w...DrChimie
The document summarizes a study on using a zirconium-based metal organic framework called UiO-66-NH2 for the removal of hexavalent chromium (Cr(VI)) from aqueous solutions. UiO-66-NH2 was synthesized using a greener approach and characterized. Batch experiments explored the effects of solution pH, adsorbent dosage, salt concentration, contact time, initial Cr(VI) concentration and temperature on adsorption. UiO-66-NH2 was able to efficiently remove Cr(VI) through adsorption and reduction. The maximum adsorption capacity from the Langmuir model was 252 mg/g. Regeneration tests showed UiO-66-NH2 had good regeneration
Analysis of Impurities in Semiconductor-Grade Hydrochloric Acid with the NexI...PerkinElmer, Inc.
This document discusses the analysis of impurities in semiconductor-grade hydrochloric acid (HCl) using inductively coupled plasma mass spectrometry (ICP-MS). HCl is commonly used to clean silicon wafers during semiconductor production, and increasingly stringent purity standards are required. ICP-MS provides the necessary low detection limits for trace metal analysis down to parts-per-trillion levels. However, polyatomic interferences from argon, oxygen, and hydrogen ions must be addressed. The PerkinElmer NexION 2000 ICP-MS uses reaction, cool plasma, and quadrupole modes to remove interferences and achieve detection limits below 1 ng/L for most elements, allowing accurate analysis of impurities in
This document summarizes an investigation into using lysine-functionalized dendrimers as potential detoxification agents for the organophosphate pesticide dichlorvos. Zeroth-, first-, and second-generation polyester dendrimers were synthesized with exactly 4, 8, and 16 lysine groups respectively on their periphery. The dendrimers were found to be water-soluble and showed low toxicity to cell lines. They were also able to efficiently capture dichlorvos, demonstrating their potential as antidotes to maintain acetylcholinesterase activity in cases of organophosphate poisoning.
Metabolomic Profiling of Spent Biomass Of Marine Microalgae, Chlorella vulgarispriyanka raviraj
OBJECTIVE:
To evaluate the presence of any high value added compounds in the spent biomass of C. vulgaris
To identify the biological activity of the extracted compounds
To evaluate the structure and nature of the compounds using Nuclear Magnetic Resonance Spectroscopy and other analytical techniques.
Development of economically viable methodologies for the simultaneous extraction of by-products from a single set of biomass.
biological activities performed -Total antioxidant capacity, Anti bacterial activity, Anti-tuberculosis activity, Anti proliferative assay
Present study aims to investigate the efficiency of newly synthesized adsorbent polyvinyl
alcohol-alginate bound nano magnetite microspheres modified with cetyltrimethyl ammonium bromide [PVAANM/CTAB]
in removal of anionic dye ‘Alizarin Red S’ from aqueous medium. The effect of agitation time,
influence of pH, amount of adsorbent, initial dye concentration and temperature were systematically studied by
batch sorption system. Various isotherms and kinetic models have been fitted with experimental data to evaluate
mechanism of adsorption. Characterization of the so-prepared adsorbent was accomplished by FTIR, XRD,
SEM and TEM analysis. The experimental data fitted very well with Freundlich and Temkin isotherm model.
The sorption kinetics follows pseudo second order kinetic model. PVA-ANM/CTAB has been found an effective,
economic, eco-friendly and efficient adsorbent as it showed ≥ 98% removal at pH 8 and could be regenerated
by acetic acid and reused.
This study analyzed aliphatic hydrocarbons in surface sediments from the Sergipe River estuarine system in Brazil. Aliphatic hydrocarbon concentrations ranged from 9.9 to 30.8 micrograms per gram of dry sediment. Carbon preference index values indicated petrogenic input at two sites, while an unresolved complex mixture was found at seven of the nine sites, suggesting multiple organic matter sources. Overall the results found a mix of natural and anthropogenic hydrocarbon inputs to the sediments, despite petroleum exploration and urban/industrial activities in the region.
The document summarizes a study that isolated and identified biosurfactant producing bacteria from Iranian oil wells. One strain, identified as Bacillus sp. NO.4, showed high salt tolerance and biosurfactant production ability over a wide pH range. Its maximum biomass production was achieved after 60 hours. GC and HPLC analysis showed the strain had the ability to degrade hydrocarbons in crude oil and completely degrade chrysene and fluorine. The isolated strains demonstrated potential for removing oil pollutants and enhanced oil recovery applications.
Similar to RHAMNOLIPID PURIFICATION USING UPLC-MS/MS (20)
Evidence of Jet Activity from the Secondary Black Hole in the OJ 287 Binary S...Sérgio Sacani
Wereport the study of a huge optical intraday flare on 2021 November 12 at 2 a.m. UT in the blazar OJ287. In the binary black hole model, it is associated with an impact of the secondary black hole on the accretion disk of the primary. Our multifrequency observing campaign was set up to search for such a signature of the impact based on a prediction made 8 yr earlier. The first I-band results of the flare have already been reported by Kishore et al. (2024). Here we combine these data with our monitoring in the R-band. There is a big change in the R–I spectral index by 1.0 ±0.1 between the normal background and the flare, suggesting a new component of radiation. The polarization variation during the rise of the flare suggests the same. The limits on the source size place it most reasonably in the jet of the secondary BH. We then ask why we have not seen this phenomenon before. We show that OJ287 was never before observed with sufficient sensitivity on the night when the flare should have happened according to the binary model. We also study the probability that this flare is just an oversized example of intraday variability using the Krakow data set of intense monitoring between 2015 and 2023. We find that the occurrence of a flare of this size and rapidity is unlikely. In machine-readable Tables 1 and 2, we give the full orbit-linked historical light curve of OJ287 as well as the dense monitoring sample of Krakow.
JAMES WEBB STUDY THE MASSIVE BLACK HOLE SEEDSSérgio Sacani
The pathway(s) to seeding the massive black holes (MBHs) that exist at the heart of galaxies in the present and distant Universe remains an unsolved problem. Here we categorise, describe and quantitatively discuss the formation pathways of both light and heavy seeds. We emphasise that the most recent computational models suggest that rather than a bimodal-like mass spectrum between light and heavy seeds with light at one end and heavy at the other that instead a continuum exists. Light seeds being more ubiquitous and the heavier seeds becoming less and less abundant due the rarer environmental conditions required for their formation. We therefore examine the different mechanisms that give rise to different seed mass spectrums. We show how and why the mechanisms that produce the heaviest seeds are also among the rarest events in the Universe and are hence extremely unlikely to be the seeds for the vast majority of the MBH population. We quantify, within the limits of the current large uncertainties in the seeding processes, the expected number densities of the seed mass spectrum. We argue that light seeds must be at least 103 to 105 times more numerous than heavy seeds to explain the MBH population as a whole. Based on our current understanding of the seed population this makes heavy seeds (Mseed > 103 M⊙) a significantly more likely pathway given that heavy seeds have an abundance pattern than is close to and likely in excess of 10−4 compared to light seeds. Finally, we examine the current state-of-the-art in numerical calculations and recent observations and plot a path forward for near-future advances in both domains.
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...Leonel Morgado
Current descriptions of immersive learning cases are often difficult or impossible to compare. This is due to a myriad of different options on what details to include, which aspects are relevant, and on the descriptive approaches employed. Also, these aspects often combine very specific details with more general guidelines or indicate intents and rationales without clarifying their implementation. In this paper we provide a method to describe immersive learning cases that is structured to enable comparisons, yet flexible enough to allow researchers and practitioners to decide which aspects to include. This method leverages a taxonomy that classifies educational aspects at three levels (uses, practices, and strategies) and then utilizes two frameworks, the Immersive Learning Brain and the Immersion Cube, to enable a structured description and interpretation of immersive learning cases. The method is then demonstrated on a published immersive learning case on training for wind turbine maintenance using virtual reality. Applying the method results in a structured artifact, the Immersive Learning Case Sheet, that tags the case with its proximal uses, practices, and strategies, and refines the free text case description to ensure that matching details are included. This contribution is thus a case description method in support of future comparative research of immersive learning cases. We then discuss how the resulting description and interpretation can be leveraged to change immersion learning cases, by enriching them (considering low-effort changes or additions) or innovating (exploring more challenging avenues of transformation). The method holds significant promise to support better-grounded research in immersive learning.
Discovery of An Apparent Red, High-Velocity Type Ia Supernova at 𝐳 = 2.9 wi...Sérgio Sacani
We present the JWST discovery of SN 2023adsy, a transient object located in a host galaxy JADES-GS
+
53.13485
−
27.82088
with a host spectroscopic redshift of
2.903
±
0.007
. The transient was identified in deep James Webb Space Telescope (JWST)/NIRCam imaging from the JWST Advanced Deep Extragalactic Survey (JADES) program. Photometric and spectroscopic followup with NIRCam and NIRSpec, respectively, confirm the redshift and yield UV-NIR light-curve, NIR color, and spectroscopic information all consistent with a Type Ia classification. Despite its classification as a likely SN Ia, SN 2023adsy is both fairly red (
�
(
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−
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)
∼
0.9
) despite a host galaxy with low-extinction and has a high Ca II velocity (
19
,
000
±
2
,
000
km/s) compared to the general population of SNe Ia. While these characteristics are consistent with some Ca-rich SNe Ia, particularly SN 2016hnk, SN 2023adsy is intrinsically brighter than the low-
�
Ca-rich population. Although such an object is too red for any low-
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cosmological sample, we apply a fiducial standardization approach to SN 2023adsy and find that the SN 2023adsy luminosity distance measurement is in excellent agreement (
≲
1
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) with
Λ
CDM. Therefore unlike low-
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Ca-rich SNe Ia, SN 2023adsy is standardizable and gives no indication that SN Ia standardized luminosities change significantly with redshift. A larger sample of distant SNe Ia is required to determine if SN Ia population characteristics at high-
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truly diverge from their low-
�
counterparts, and to confirm that standardized luminosities nevertheless remain constant with redshift.
CLASS 12th CHEMISTRY SOLID STATE ppt (Animated)eitps1506
Description:
Dive into the fascinating realm of solid-state physics with our meticulously crafted online PowerPoint presentation. This immersive educational resource offers a comprehensive exploration of the fundamental concepts, theories, and applications within the realm of solid-state physics.
From crystalline structures to semiconductor devices, this presentation delves into the intricate principles governing the behavior of solids, providing clear explanations and illustrative examples to enhance understanding. Whether you're a student delving into the subject for the first time or a seasoned researcher seeking to deepen your knowledge, our presentation offers valuable insights and in-depth analyses to cater to various levels of expertise.
Key topics covered include:
Crystal Structures: Unravel the mysteries of crystalline arrangements and their significance in determining material properties.
Band Theory: Explore the electronic band structure of solids and understand how it influences their conductive properties.
Semiconductor Physics: Delve into the behavior of semiconductors, including doping, carrier transport, and device applications.
Magnetic Properties: Investigate the magnetic behavior of solids, including ferromagnetism, antiferromagnetism, and ferrimagnetism.
Optical Properties: Examine the interaction of light with solids, including absorption, reflection, and transmission phenomena.
With visually engaging slides, informative content, and interactive elements, our online PowerPoint presentation serves as a valuable resource for students, educators, and enthusiasts alike, facilitating a deeper understanding of the captivating world of solid-state physics. Explore the intricacies of solid-state materials and unlock the secrets behind their remarkable properties with our comprehensive presentation.
PPT on Direct Seeded Rice presented at the three-day 'Training and Validation Workshop on Modules of Climate Smart Agriculture (CSA) Technologies in South Asia' workshop on April 22, 2024.
PPT on Alternate Wetting and Drying presented at the three-day 'Training and Validation Workshop on Modules of Climate Smart Agriculture (CSA) Technologies in South Asia' workshop on April 22, 2024.
Microbial interaction
Microorganisms interacts with each other and can be physically associated with another organisms in a variety of ways.
One organism can be located on the surface of another organism as an ectobiont or located within another organism as endobiont.
Microbial interaction may be positive such as mutualism, proto-cooperation, commensalism or may be negative such as parasitism, predation or competition
Types of microbial interaction
Positive interaction: mutualism, proto-cooperation, commensalism
Negative interaction: Ammensalism (antagonism), parasitism, predation, competition
I. Mutualism:
It is defined as the relationship in which each organism in interaction gets benefits from association. It is an obligatory relationship in which mutualist and host are metabolically dependent on each other.
Mutualistic relationship is very specific where one member of association cannot be replaced by another species.
Mutualism require close physical contact between interacting organisms.
Relationship of mutualism allows organisms to exist in habitat that could not occupied by either species alone.
Mutualistic relationship between organisms allows them to act as a single organism.
Examples of mutualism:
i. Lichens:
Lichens are excellent example of mutualism.
They are the association of specific fungi and certain genus of algae. In lichen, fungal partner is called mycobiont and algal partner is called
II. Syntrophism:
It is an association in which the growth of one organism either depends on or improved by the substrate provided by another organism.
In syntrophism both organism in association gets benefits.
Compound A
Utilized by population 1
Compound B
Utilized by population 2
Compound C
utilized by both Population 1+2
Products
In this theoretical example of syntrophism, population 1 is able to utilize and metabolize compound A, forming compound B but cannot metabolize beyond compound B without co-operation of population 2. Population 2is unable to utilize compound A but it can metabolize compound B forming compound C. Then both population 1 and 2 are able to carry out metabolic reaction which leads to formation of end product that neither population could produce alone.
Examples of syntrophism:
i. Methanogenic ecosystem in sludge digester
Methane produced by methanogenic bacteria depends upon interspecies hydrogen transfer by other fermentative bacteria.
Anaerobic fermentative bacteria generate CO2 and H2 utilizing carbohydrates which is then utilized by methanogenic bacteria (Methanobacter) to produce methane.
ii. Lactobacillus arobinosus and Enterococcus faecalis:
In the minimal media, Lactobacillus arobinosus and Enterococcus faecalis are able to grow together but not alone.
The synergistic relationship between E. faecalis and L. arobinosus occurs in which E. faecalis require folic acid
When I was asked to give a companion lecture in support of ‘The Philosophy of Science’ (https://shorturl.at/4pUXz) I decided not to walk through the detail of the many methodologies in order of use. Instead, I chose to employ a long standing, and ongoing, scientific development as an exemplar. And so, I chose the ever evolving story of Thermodynamics as a scientific investigation at its best.
Conducted over a period of >200 years, Thermodynamics R&D, and application, benefitted from the highest levels of professionalism, collaboration, and technical thoroughness. New layers of application, methodology, and practice were made possible by the progressive advance of technology. In turn, this has seen measurement and modelling accuracy continually improved at a micro and macro level.
Perhaps most importantly, Thermodynamics rapidly became a primary tool in the advance of applied science/engineering/technology, spanning micro-tech, to aerospace and cosmology. I can think of no better a story to illustrate the breadth of scientific methodologies and applications at their best.
1. Insert your information hereIntroduction Results
References
Biousurfactants are surface-active agents, whose potential application
range includes the use in bioremediation of hydrocarbon contaminated
soils. (Behrens et al. 2016). Rhamnolipids (RLs), the most studied type
of biosurfactants, makes hydrocarbons soluble for degradation through
cell activity. RL diversity arises from the heterogeneous congener
mixtures synthesized which can vary in alkyl chain length ranging from
C8 to C14 and their isomers for mono or di-rhamnolipids, raising more
than 60 different congeners. The aim of this work was to know the
diversity of the RL congeners produced by the Pseudomonas
aeruginosa 6K-11 strain and detected using ultra pressure liquid
chromatography tandem mass spectrometry (UPLC-MS/MS) method.
Purification and determination of Rhamnolipid congeners through ultra-performance liquid
chromatography tandem mass spectrometry (UPLC-MS/MS), employing corn oil as carbon source
M.V.G. Alván,K.K.Valladares,D.G.Martinez,F.Merino,S.Gutierrez.
U.N.M.S.M.-LaboratoryofMicrobiologyand MicrobialBiotechnology.Lima-PE
mgracial13@gmail.com
Methods
Results
MONO-
RHAMNOLIPID
CONGENER
TRANSITION
% ABUNDANCE
SEP-SN AMB-SN PA-T
Rha-C8-C10 475 - 305 0 0 1.90
Rha-C10-C8 475 - 333 0 0.00 2.03
Rha-C10-C10 503 - 333 0 82.87 80.61
Rha-C10-C12:1 529 - 333 0 8.57 8.26
Rha-C121:-C10 529 - 359 0 0 0.20
Rha-C10-C12 531 - 333 0 8.55 5.18
Rha-C12-C10 531 - 361 0 0 1.81
Total 0 100% 100%
DI-RHAMNOLIPID
CONGENER
Rha-Rha-C8-C10 621 - 451 0 1.26 1.15
Rha-Rha-C10-C8 621 - 479 59.04 1.99 1.36
Rha-Rha-C10-C10 649 - 479 40.96 68.44 74.42
Rha-Rha-C10-C12:1 675 - 479 0 9.73 6.54
Rha-Rha-C12:1-C10 675 - 505 0 0 0.22
Rha-Rha-C10-C12 677 - 479 0 14.76 12.01
Rha-Rha-C12-C10 677 - 507 0 3.83 4.30
Total 100% 100% 100%
Conclusion
Table 1. % Relative abundance was calculated based on the individual congener peak area divided by the sum total area for all RLs for either
mono- or di- RL fractions as per current practice for published literature in this area.
This study showed that the congener produced and the nitrogen source are
not related. Also, the best method to purify rhamnolipids depends on the
purpose of the research, since the purification through acid precipitation is
cost effective, fast and the 14 congeners are obtained, but there is still
contaminating molecules. On the other hand, adsorption and ion exchange
chromatography reduce the abundance of contaminating molecules and, in
the case of using both techniques, 2 rhamnolipids congeners are obtained.
0 10 20 30 40 50 60 70
Rha-Rha-C10-C8
Rha-Rha-C8-C10
Rha-C10-C8
Rha-C8-C10
Rha-Rha-C10-C10
Rha-C10-C10
Rha-Rha-C10-C12:1
Rha-Rha-C12:1-C10
Rha-C10-C12:1
Rha-C121:-C10
Rha-Rha-C10-C12
Rha-Rha-C12-C10
Rha-C12-C10
Rha-C10-C12
% ABUNDANCE
RLCONGENER
RL Congeners detected using UPLC MS/MS
PA-T AMB-SN SEP-SN
Graphic 1. Abundance percentage of RL congener detected in different samples. PA-T: tryptone as nitrogen source and purification with acid
precipitation method. AMB-SN: Sodium Nitrate as nitrogen source, and purified trough adsorption chromatography. SEP-SN: Sodium Nitrate as nitrogen
source, and purified trough adsorption and ion exchange chromatography.Rudden, M.2015. Applied microbiology and biotechnology.
Behrens, B.2016.Analytical and bioanalytical chemistry,.
Reiling, H 1986 Applied and environmental microbiology Acknowledges: Supported by Proyecto FINCYT under Contract Nº238-IA-2013
Methods
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