This document contains slides and information about microbiological testing and identification of various bacteria and fungi. It includes schemes for staining bacteria with and without background staining. It also includes images of various microbes growing on different media like blood agar, chocolate agar and Loffler's serum. Various tests are described like coagulase testing of Staphylococcus, antibiotic sensitivity testing, phage typing and serological testing like Elek's test and tube agglutination testing.

2. SLIDES

3. Schemes for slides
With background Without background
Gram positive cocci Gram positive bacilli Gram negative cocci

4. Scheme for slides
With background Without background
Brown Blue Red
Organism Gram +ve
cocci
Organism Gram -ve

5. Staphylococci in culture

6. Staphylococci in culture

7. Streptococci in culture

8. Streptococci in culture

9. Candida

10. Neisseria in culture

11. Corynebacterium diphtheriae

12. Clostridium tetani

13. Clostridium tetani

14. Staphylococci in pus

15. Staphylococci in Pus

16. Streptococci in Pus

17. Streptococci in pus

18. Vincent’s angina

19. Mycobacterium tuberculosis
(Acid Fast bacilli Z-N stain)

20. Mycobacterium tuberculosis
(Acid Fast bacilli Z-N stain)

21. Mycobacterium tuberculosis
(Acid Fast bacilli Z-N stain)

22. Spirochaetes
(Fontana stain)

23. Spirochaetes (fontana stain)

24. Spirochaetes (fontana stain)

25. MEDIA

26. Nutrient agar

27. Staphylococcus aureus on nutrient agar
( showing golden yellow endopigment )

28. Blood agar

29. Types of hemolysis on Blood agar

30. Alpha-hemolysis on blood agar

31. Beta-hemolysis on blood agar

32. Non-hemolysis on blood agar

33. Chocolate agar
for Neisseria

34. Loffler’s serum
for C. diphtheriae

35. Lowenstein Jensen medium
for M. tuberculosis

36. Cooked meat medium
for anaerobic cultivation

37. TESTS

38. Coagulase Test
Staphylococcus aureus
Coagulase negative
staphylococci,
e.g. S. epidermidis

39. Coagulase test
Staphylococcus aureus Coagulase negative staphylococci,
e.g. S. epidermidis

40. Antibiotic Sensitivity Test
(Disc diffusion)
Sensitive to:
1, 2, 3, 4, 6
Resistant to:
5
1
5
62
4
3

41. Antibiotic Sensitivity Test
(Disc diffusion)
Sensitive to:
1, 2, 5, 6
Resistant to:
3, 4
1
5
6
2
4
3

42. Phage typing
1 2
3 4 5 6
7 8 9 10
11 12 13 14
15 16 17 18
19 20
Sensitive to 4, 5, 6,……..etc.

43. SEROLOGY

44. Elek’s Test
Toxigenic C. diphtheriae Non-toxigenic C. diphtheriae

45. Simple tube agglutination test
(Titre:1/320)
TITRE
1/20 1/40 1/80 1/160 1/320 1/640 1/1280 - ve control