This document summarizes several common diseases and their microbial diagnosis techniques. It discusses typhoid, tuberculosis, malaria, cholera, hepatitis, meningitis, syphilis and gonorrhea. For each disease, it describes the causative organism, traditional diagnosis methods like culture and microscopy, and newer techniques such as immunoassays, PCR and nucleic acid amplification tests that provide faster and more accurate detection of pathogens.
recent microbial techniques & advancement in identifying, cultivating,& handl...Karunanidhan3
This document discusses various techniques used to identify and diagnose microorganisms and diseases. It begins by describing microscopic organisms and methods to identify microbes, including staining methods, biochemical tests, PCR, and spectrometric techniques. It then provides details on specific diagnostic techniques for diseases like typhoid, tuberculosis, malaria, cholera, hepatitis, meningitis, syphilis, gonorrhea, HIV/AIDS and diphtheria. These include culture-based techniques, molecular tests like PCR, serological tests, and rapid diagnostic tests. The document emphasizes the importance of accurate identification of pathogens for effective disease diagnosis and treatment.
Malaria is caused by a parasite transmitted through mosquito bites. It causes symptoms like fever and can become life-threatening. There are several laboratory tests to diagnose malaria including blood smear tests, PCR, and rapid diagnostic tests. The QBC test is a fluorescence microscopy-based test that uses centrifugation to separate blood components by density, making the malaria parasites visible under a fluorescent microscope as they stain brightly. It is more sensitive than blood smear tests and can detect malaria earlier.
This document provides guidelines for prescribing medications during pregnancy and breastfeeding. It discusses how pregnancy causes physiological changes that can impact drug absorption, distribution, metabolism, and excretion, potentially requiring dosage adjustments. Specific medications that are considered safe to use during pregnancy are outlined, including antibiotics, analgesics, asthma medications, and treatments for common conditions like iron deficiency anemia and constipation. The document also notes that while many drugs pass into breastmilk, few are generally present in amounts that could harm infants, though some drugs like phenobarbital can concentrate in breastmilk and certain others are not recommended.
this presentation gives informationabout microbial assay of vitamins B2 and B12. it is based upon the guidelines of indian pharmacopoeia. this presentation highlights the principle, process and applications of microbial assay
This document discusses sterility testing protocols for pharmaceutical products as per Indian Pharmacopeia guidelines. It defines sterility testing as testing to confirm absence of viable microorganisms. Sterility testing is important for medical devices and preparations like ophthalmic, injections, implants etc. The test is based on principle that microorganisms will grow in nutritive media at favorable temperature. There are two methods for sterility test - membrane filtration method suitable for liquids and direct inoculation method where samples are directly inoculated to culture media. The document discusses the different culture media and quantities of samples used based on product type.
This document discusses the significance of toxoids in active immunity. It defines key terms like vaccines, toxoids, live vaccines, attenuated live vaccines, inactivated vaccines, polysaccharide vaccines, and surface antigen vaccines. It explains that toxoids create immunity to the toxins produced by pathogens rather than the pathogens themselves. Various routes of administration are outlined including subcutaneous, intramuscular, oral, intradermal, and intranasal. Vaccination schedules for primary vaccination and booster doses are also summarized.
recent microbial techniques & advancement in identifying, cultivating,& handl...Karunanidhan3
This document discusses various techniques used to identify and diagnose microorganisms and diseases. It begins by describing microscopic organisms and methods to identify microbes, including staining methods, biochemical tests, PCR, and spectrometric techniques. It then provides details on specific diagnostic techniques for diseases like typhoid, tuberculosis, malaria, cholera, hepatitis, meningitis, syphilis, gonorrhea, HIV/AIDS and diphtheria. These include culture-based techniques, molecular tests like PCR, serological tests, and rapid diagnostic tests. The document emphasizes the importance of accurate identification of pathogens for effective disease diagnosis and treatment.
Malaria is caused by a parasite transmitted through mosquito bites. It causes symptoms like fever and can become life-threatening. There are several laboratory tests to diagnose malaria including blood smear tests, PCR, and rapid diagnostic tests. The QBC test is a fluorescence microscopy-based test that uses centrifugation to separate blood components by density, making the malaria parasites visible under a fluorescent microscope as they stain brightly. It is more sensitive than blood smear tests and can detect malaria earlier.
This document provides guidelines for prescribing medications during pregnancy and breastfeeding. It discusses how pregnancy causes physiological changes that can impact drug absorption, distribution, metabolism, and excretion, potentially requiring dosage adjustments. Specific medications that are considered safe to use during pregnancy are outlined, including antibiotics, analgesics, asthma medications, and treatments for common conditions like iron deficiency anemia and constipation. The document also notes that while many drugs pass into breastmilk, few are generally present in amounts that could harm infants, though some drugs like phenobarbital can concentrate in breastmilk and certain others are not recommended.
this presentation gives informationabout microbial assay of vitamins B2 and B12. it is based upon the guidelines of indian pharmacopoeia. this presentation highlights the principle, process and applications of microbial assay
This document discusses sterility testing protocols for pharmaceutical products as per Indian Pharmacopeia guidelines. It defines sterility testing as testing to confirm absence of viable microorganisms. Sterility testing is important for medical devices and preparations like ophthalmic, injections, implants etc. The test is based on principle that microorganisms will grow in nutritive media at favorable temperature. There are two methods for sterility test - membrane filtration method suitable for liquids and direct inoculation method where samples are directly inoculated to culture media. The document discusses the different culture media and quantities of samples used based on product type.
This document discusses the significance of toxoids in active immunity. It defines key terms like vaccines, toxoids, live vaccines, attenuated live vaccines, inactivated vaccines, polysaccharide vaccines, and surface antigen vaccines. It explains that toxoids create immunity to the toxins produced by pathogens rather than the pathogens themselves. Various routes of administration are outlined including subcutaneous, intramuscular, oral, intradermal, and intranasal. Vaccination schedules for primary vaccination and booster doses are also summarized.
This document discusses drug-induced pulmonary diseases. It outlines several mechanisms by which drugs can cause lung injury, including direct cytotoxic effects, oxidative injury, and immune-mediated reactions. It describes different types of drug-induced lung diseases such as interstitial lung disease, pulmonary edema, bronchospasm, and pulmonary hypertension. Specific drugs that can induce these diseases and their mechanisms are discussed. Diagnosis and treatment approaches for drug-induced pulmonary conditions are also summarized.
The document outlines various methods used to test the efficacy of disinfectants, including carrier tests, suspension tests, and practical tests. Carrier tests involve contaminating a thread with bacteria and exposing it to disinfectants. Suspension tests measure a disinfectant's ability to kill bacteria suspended in its solution. Practical tests evaluate disinfectants under real-world conditions. The document also describes the phenol coefficient test, which compares a disinfectant's effectiveness to that of phenol, and the filter paper test, which detects zones of bacterial inhibition around treated disks.
It is an antibiotic that was the first drug to be successful against tuberculosis but now it is used with other drugs because of its toxic effects
othere uses
Anti TB drug
Plaque
Veterinary treatment against gram negative bacteria in horses , cattle , sheep
Fermentation is defined as chemical transformation of organic compound brought about through agency of microorganisms
Microorganisms useful in fementation can be either prokaryotes such as bacteria , virus or eukaryotes such as fungi; yeast
Test Organism-Streptomyces griseus
The document summarizes the Schick test, which is used to determine susceptibility to diphtheria. The test involves injecting diluted diphtheria toxin intradermally and observing the reaction. If a red, swollen rash develops around the injection site within 2-4 days, the person is susceptible to diphtheria. Now outdated due to widespread vaccination, the Schick test worked by comparing the reaction at the toxin injection site versus the control site injected with inactivated toxin. A positive test indicated the need for diphtheria vaccination to develop protective immunity.
This document discusses several common diagnostic tests: the peripheral blood smear, Mantoux test, and quantitative buffy coat (QBC) test. The peripheral blood smear involves spreading blood on a slide to examine blood cells under a microscope. The Mantoux test uses a purified protein derivative injected under the skin to detect tuberculosis infection. The QBC test uses centrifugation and fluorescent staining to examine blood cells and detect malaria or other blood parasites. These tests provide useful diagnostic information by examining blood components like cells, parasites, and reactions to antigens.
This document summarizes sterility testing procedures for pharmaceutical products. Sterility testing aims to detect any viable microorganisms that may be present. Samples are inoculated into fluid thioglycollate medium, alternative thioglycollate medium, or soybean-casein digest medium and incubated with test microbes like S. aureus, C. sporogenes, P. aeruginosa, B. subtilis, A. brasiliensis or C. albicans. Tests are done using either membrane filtration or direct inoculation methods depending on the product type and volume. After incubation, the results are observed and interpreted to determine if the product passes or fails sterility requirements.
This document discusses drugs used to treat leprosy (Hansen's disease). It outlines the mechanisms and uses of major antileprotics including dapsone, rifampicin, and clofazimine. It also discusses alternative drugs like fluoroquinolones and various multidrug therapy regimens. Treatment strategies aim to prevent resistance and quickly relieve symptoms. Reactions like lepra reactions are also covered.
This document discusses HIV/AIDS and opportunistic infections. It begins by defining HIV and AIDS, describing how HIV damages the immune system over time leading to AIDS. It then covers the epidemiology, symptoms, pathophysiology and stages of HIV infection. It discusses transmission methods and treatment, focusing on highly active antiretroviral therapy (HAART) and classes of antiretroviral drugs. The document concludes by examining common opportunistic infections that take advantage of a weakened immune system in AIDS patients.
This document discusses different types of allergies including food allergy, drug allergy, and chemical allergy. Food allergy is an immunologic reaction to specific food proteins with symptoms that can include hives, difficulty breathing, and anaphylaxis. Common food allergens for children are milk, eggs, peanuts, wheat and soy, while for adults it is peanuts, tree nuts, fish and shellfish. Drug allergy is an immunologic response to pharmaceutical agents with risk factors like age, genetics and previous drug reactions. Common causes are antibiotics, aspirin, and chemotherapy. Chemical allergy involves hypersensitivity to chemicals like formaldehyde, pesticides and solvents. Diagnosis is via tests while treatment
A booster dose is an additional administration of a vaccine that provides a re-exposure to the immunizing antigen in order to increase immunity against that antigen after memory has declined over time. The need for a booster is evaluated by measuring antibody levels and anamnestic response years after the primary dose. While some vaccines provide lifelong immunity, others like tetanus require boosters every 10 years as memory cells that provide protection decrease over time. Booster doses have also been recommended for COVID-19 vaccines to increase protection against variants as vaccine efficacy declines after around 6 months.
The document provides information about two medical diagnostic techniques: the quantitative buffy coat (QBC) test and enzyme-linked immunosorbent assay (ELISA). It describes the QBC test procedure which uses a stained capillary tube to detect malaria parasites in centrifuged blood samples based on their fluorescence. It also gives an overview of the principles and types of ELISA, including indirect, sandwich, and competitive ELISA, which are immunochemical techniques used to detect and quantify antigens or antibodies.
Nitrates are prodrugs that release nitric oxide (NO) in the body. Common nitrate drugs include nitroglycerin and isosorbide mononitrate. Nitrates were first synthesized in the 1840s and are used to treat conditions like angina by relaxing smooth muscle and dilating blood vessels. They reduce blood pressure and myocardial oxygen demand. However, tolerance to nitrates develops rapidly due to desensitization of vascular smooth muscle. Providing a daily nitrate-free period can help overcome tolerance. Nitrates are also found naturally in soil and water and excess nitrates can cause methemoglobinemia or be linked to cancer.
UTIs are caused by bacterial infections in the urinary tract. They range from cystitis, an infection of the bladder, to pyelonephritis, a serious infection of the kidneys. The most common pathogen is E. coli. Treatment depends on the severity and location of the infection, with acute uncomplicated cystitis usually treated with a 3 day course of antibiotics like cotrimoxazole or cephalexin. More serious or complicated infections may require 7-14 days of treatment with intravenous antibiotics such as gentamicin and third generation cephalosporins. Chronic infections necessitate long term suppressive antibiotic therapy after identifying and addressing any underlying causes.
This document provides notes on immunopharmacology and immunosuppressant drugs. It begins with an overview of the immune system including cell-mediated and antibody-mediated immunity. It then discusses various classes of immunosuppressant drugs including calcineurin inhibitors cyclosporine and tacrolimus, glucocorticoids like prednisolone, mTOR inhibitors, antiproliferatives, biological agents, and monoclonal antibodies. For selected drugs, their mechanisms of action, indications, pharmacokinetics, adverse effects and drug interactions are described in detail.
The document discusses various methods for testing the efficacy of disinfectants, including:
1. Koch's method, which tests the ability of a disinfectant to kill Bacillus anthraces spores.
2. Rideal Walker and Chick-Martin tests, which determine the phenol coefficient of a disinfectant by comparing its bactericidal effects to phenol under clean and dirty conditions.
3. In-use and capacity use dilution tests assess the ability of a disinfectant to kill microbes when diluted in conditions mimicking actual use over time in the presence of organic matter.
4. No single test can reliably determine a disinfectant's efficacy
This document provides an overview of pharmacotherapy for asthma. It defines asthma as a chronic inflammatory disease of the airways characterized by increased responsiveness to stimuli. Asthma can be triggered by environmental and genetic factors. The main types of asthma and treatments discussed include bronchodilators, leukotriene antagonists, mast cell stabilizers, corticosteroids, anti-IgE antibody therapy, and recent advances in immunotherapy.
Hyperlipidemia and drug therapy for hyperlipidemiaakbar siddiq
This document discusses hyperlipidemia and drug therapy for hyperlipidemia. It begins by defining hyperlipidemia and describing the main types of lipoproteins and their roles in cholesterol transport. It then discusses the diagnosis and management of hyperlipidemias, including lifestyle modifications like diet and exercise as well as the major classes of drug therapy like statins, fibrates, bile acid sequestrants, and nicotinic acid. The mechanisms of action, uses, and side effects of each drug class are summarized. Combination drug therapy is also addressed when single drug therapy is insufficient.
This document outlines the diagnostic approach and laboratory tests for evaluating a patient presenting with pyrexia of unknown origin (PUO). It describes collecting relevant clinical history and performing a physical exam. Specimens including blood, urine, sputum, CSF and tissues may be obtained for bacterial, viral, parasitic and fungal cultures and stains. Tests like blood cultures, urine cultures, sputum smears and cultures, and CSF analysis can help identify potential infectious causes. Serology, skin tests, hematology, immunology and biopsy may also provide diagnostic clues. Empiric antibiotic therapy is guided by risk factors and test results.
This document provides information on the bacteria Neisseria gonorrhoeae and Neisseria meningitidis. It discusses their classification, characteristics, pathogenesis, diagnosis and treatment. Key points include:
- They are Gram-negative diplococci within the family Neisseriaceae. N. meningitidis is encapsulated while N. gonorrhoeae is not.
- N. meningitidis is a common cause of bacterial meningitis while N. gonorrhoeae causes the sexually transmitted infection gonorrhea.
- Diagnosis involves culture, antigen detection and PCR. Treatment for gonorrhea is typically ceftriaxone or ciprofloxacin along
This document discusses drug-induced pulmonary diseases. It outlines several mechanisms by which drugs can cause lung injury, including direct cytotoxic effects, oxidative injury, and immune-mediated reactions. It describes different types of drug-induced lung diseases such as interstitial lung disease, pulmonary edema, bronchospasm, and pulmonary hypertension. Specific drugs that can induce these diseases and their mechanisms are discussed. Diagnosis and treatment approaches for drug-induced pulmonary conditions are also summarized.
The document outlines various methods used to test the efficacy of disinfectants, including carrier tests, suspension tests, and practical tests. Carrier tests involve contaminating a thread with bacteria and exposing it to disinfectants. Suspension tests measure a disinfectant's ability to kill bacteria suspended in its solution. Practical tests evaluate disinfectants under real-world conditions. The document also describes the phenol coefficient test, which compares a disinfectant's effectiveness to that of phenol, and the filter paper test, which detects zones of bacterial inhibition around treated disks.
It is an antibiotic that was the first drug to be successful against tuberculosis but now it is used with other drugs because of its toxic effects
othere uses
Anti TB drug
Plaque
Veterinary treatment against gram negative bacteria in horses , cattle , sheep
Fermentation is defined as chemical transformation of organic compound brought about through agency of microorganisms
Microorganisms useful in fementation can be either prokaryotes such as bacteria , virus or eukaryotes such as fungi; yeast
Test Organism-Streptomyces griseus
The document summarizes the Schick test, which is used to determine susceptibility to diphtheria. The test involves injecting diluted diphtheria toxin intradermally and observing the reaction. If a red, swollen rash develops around the injection site within 2-4 days, the person is susceptible to diphtheria. Now outdated due to widespread vaccination, the Schick test worked by comparing the reaction at the toxin injection site versus the control site injected with inactivated toxin. A positive test indicated the need for diphtheria vaccination to develop protective immunity.
This document discusses several common diagnostic tests: the peripheral blood smear, Mantoux test, and quantitative buffy coat (QBC) test. The peripheral blood smear involves spreading blood on a slide to examine blood cells under a microscope. The Mantoux test uses a purified protein derivative injected under the skin to detect tuberculosis infection. The QBC test uses centrifugation and fluorescent staining to examine blood cells and detect malaria or other blood parasites. These tests provide useful diagnostic information by examining blood components like cells, parasites, and reactions to antigens.
This document summarizes sterility testing procedures for pharmaceutical products. Sterility testing aims to detect any viable microorganisms that may be present. Samples are inoculated into fluid thioglycollate medium, alternative thioglycollate medium, or soybean-casein digest medium and incubated with test microbes like S. aureus, C. sporogenes, P. aeruginosa, B. subtilis, A. brasiliensis or C. albicans. Tests are done using either membrane filtration or direct inoculation methods depending on the product type and volume. After incubation, the results are observed and interpreted to determine if the product passes or fails sterility requirements.
This document discusses drugs used to treat leprosy (Hansen's disease). It outlines the mechanisms and uses of major antileprotics including dapsone, rifampicin, and clofazimine. It also discusses alternative drugs like fluoroquinolones and various multidrug therapy regimens. Treatment strategies aim to prevent resistance and quickly relieve symptoms. Reactions like lepra reactions are also covered.
This document discusses HIV/AIDS and opportunistic infections. It begins by defining HIV and AIDS, describing how HIV damages the immune system over time leading to AIDS. It then covers the epidemiology, symptoms, pathophysiology and stages of HIV infection. It discusses transmission methods and treatment, focusing on highly active antiretroviral therapy (HAART) and classes of antiretroviral drugs. The document concludes by examining common opportunistic infections that take advantage of a weakened immune system in AIDS patients.
This document discusses different types of allergies including food allergy, drug allergy, and chemical allergy. Food allergy is an immunologic reaction to specific food proteins with symptoms that can include hives, difficulty breathing, and anaphylaxis. Common food allergens for children are milk, eggs, peanuts, wheat and soy, while for adults it is peanuts, tree nuts, fish and shellfish. Drug allergy is an immunologic response to pharmaceutical agents with risk factors like age, genetics and previous drug reactions. Common causes are antibiotics, aspirin, and chemotherapy. Chemical allergy involves hypersensitivity to chemicals like formaldehyde, pesticides and solvents. Diagnosis is via tests while treatment
A booster dose is an additional administration of a vaccine that provides a re-exposure to the immunizing antigen in order to increase immunity against that antigen after memory has declined over time. The need for a booster is evaluated by measuring antibody levels and anamnestic response years after the primary dose. While some vaccines provide lifelong immunity, others like tetanus require boosters every 10 years as memory cells that provide protection decrease over time. Booster doses have also been recommended for COVID-19 vaccines to increase protection against variants as vaccine efficacy declines after around 6 months.
The document provides information about two medical diagnostic techniques: the quantitative buffy coat (QBC) test and enzyme-linked immunosorbent assay (ELISA). It describes the QBC test procedure which uses a stained capillary tube to detect malaria parasites in centrifuged blood samples based on their fluorescence. It also gives an overview of the principles and types of ELISA, including indirect, sandwich, and competitive ELISA, which are immunochemical techniques used to detect and quantify antigens or antibodies.
Nitrates are prodrugs that release nitric oxide (NO) in the body. Common nitrate drugs include nitroglycerin and isosorbide mononitrate. Nitrates were first synthesized in the 1840s and are used to treat conditions like angina by relaxing smooth muscle and dilating blood vessels. They reduce blood pressure and myocardial oxygen demand. However, tolerance to nitrates develops rapidly due to desensitization of vascular smooth muscle. Providing a daily nitrate-free period can help overcome tolerance. Nitrates are also found naturally in soil and water and excess nitrates can cause methemoglobinemia or be linked to cancer.
UTIs are caused by bacterial infections in the urinary tract. They range from cystitis, an infection of the bladder, to pyelonephritis, a serious infection of the kidneys. The most common pathogen is E. coli. Treatment depends on the severity and location of the infection, with acute uncomplicated cystitis usually treated with a 3 day course of antibiotics like cotrimoxazole or cephalexin. More serious or complicated infections may require 7-14 days of treatment with intravenous antibiotics such as gentamicin and third generation cephalosporins. Chronic infections necessitate long term suppressive antibiotic therapy after identifying and addressing any underlying causes.
This document provides notes on immunopharmacology and immunosuppressant drugs. It begins with an overview of the immune system including cell-mediated and antibody-mediated immunity. It then discusses various classes of immunosuppressant drugs including calcineurin inhibitors cyclosporine and tacrolimus, glucocorticoids like prednisolone, mTOR inhibitors, antiproliferatives, biological agents, and monoclonal antibodies. For selected drugs, their mechanisms of action, indications, pharmacokinetics, adverse effects and drug interactions are described in detail.
The document discusses various methods for testing the efficacy of disinfectants, including:
1. Koch's method, which tests the ability of a disinfectant to kill Bacillus anthraces spores.
2. Rideal Walker and Chick-Martin tests, which determine the phenol coefficient of a disinfectant by comparing its bactericidal effects to phenol under clean and dirty conditions.
3. In-use and capacity use dilution tests assess the ability of a disinfectant to kill microbes when diluted in conditions mimicking actual use over time in the presence of organic matter.
4. No single test can reliably determine a disinfectant's efficacy
This document provides an overview of pharmacotherapy for asthma. It defines asthma as a chronic inflammatory disease of the airways characterized by increased responsiveness to stimuli. Asthma can be triggered by environmental and genetic factors. The main types of asthma and treatments discussed include bronchodilators, leukotriene antagonists, mast cell stabilizers, corticosteroids, anti-IgE antibody therapy, and recent advances in immunotherapy.
Hyperlipidemia and drug therapy for hyperlipidemiaakbar siddiq
This document discusses hyperlipidemia and drug therapy for hyperlipidemia. It begins by defining hyperlipidemia and describing the main types of lipoproteins and their roles in cholesterol transport. It then discusses the diagnosis and management of hyperlipidemias, including lifestyle modifications like diet and exercise as well as the major classes of drug therapy like statins, fibrates, bile acid sequestrants, and nicotinic acid. The mechanisms of action, uses, and side effects of each drug class are summarized. Combination drug therapy is also addressed when single drug therapy is insufficient.
This document outlines the diagnostic approach and laboratory tests for evaluating a patient presenting with pyrexia of unknown origin (PUO). It describes collecting relevant clinical history and performing a physical exam. Specimens including blood, urine, sputum, CSF and tissues may be obtained for bacterial, viral, parasitic and fungal cultures and stains. Tests like blood cultures, urine cultures, sputum smears and cultures, and CSF analysis can help identify potential infectious causes. Serology, skin tests, hematology, immunology and biopsy may also provide diagnostic clues. Empiric antibiotic therapy is guided by risk factors and test results.
This document provides information on the bacteria Neisseria gonorrhoeae and Neisseria meningitidis. It discusses their classification, characteristics, pathogenesis, diagnosis and treatment. Key points include:
- They are Gram-negative diplococci within the family Neisseriaceae. N. meningitidis is encapsulated while N. gonorrhoeae is not.
- N. meningitidis is a common cause of bacterial meningitis while N. gonorrhoeae causes the sexually transmitted infection gonorrhea.
- Diagnosis involves culture, antigen detection and PCR. Treatment for gonorrhea is typically ceftriaxone or ciprofloxacin along
2nd term lecture,_,vib,_helico,tb,_spiro,rick[1]عادل الحربي
The document discusses various bacteria including Haemophilus influenzae, which causes diseases like pneumonia but is difficult to diagnose; it also covers Mycobacterium tuberculosis and the symptoms and treatment of tuberculosis; additionally, it summarizes different types of spirochetes, rickettsiae, and how they are transmitted and can cause diseases.
lab diagnosis of viral infections - mayuri.pptxDrmayuribhise
T.M. River, 1937
Modified from Koch’s Postulates (proof of bacterial diseases)
Isolate virus from diseased hosts.
Cultivation of virus in host cells.
Proof of filterability.
Production of a comparable disease when the cultivated virus is used to infect experimental animals.
Reisolation of the same virus from the infected experimental animal.
Detection of a specific immune response to the virus.
Much more expensive and difficult to study animal viruses than bacteriophages
Cultivation in host cells
Living animal
Embryonated chicken eggs
Cell or tissue culture (= in vitro)
Over 60% of all infectious disease cases seen by a physician are due to viral infections.
Quality of patient specimens and their transport to the laboratory is importantViral Diagnostics in the Clinical Laboratory
Types of specimens:-
Respiratory tract infections: Nasal and bronchial washings, throat and nasal swabs, sputum
Eye infections: throat and Conjunctival swab/scraping
Gastrointestinal tract infections: stool and rectal swabs
Vesicular rash: vesicle fluid, skin scrapings
Maculopapular rash: throat, stool, and rectal swabs
CNS (encephalitis and meningitis cases): stool, tissue, saliva, brain biopsy, cerebrospinal fluid
Genital infections: vesicle fluid or swab
Urinary tract infections: urine
Blood borne infections: blood
Sterile, leak proof container
Minimal interval
Transport media
Viral infusion broth (VIB)
Sucrose-phosphate-glutamate (SPG)
Storage temperature:
4 deg C for up to 96 hours
Minus 70 deg C beyond 96 hours
Repeated cycles of freezing and thawing to be avoided
106 virus particles per ml required for visualization,
50,000 - 60,000 magnification normally used.Specimens are negatively stained by Potassium phosphotungstate and scanned under EM
Viruses may be detected in the following specimens.
Virus particles are detected and identified on the basis of morphology.
A) Shape
Rabiesvirus –bullet shaped
Rotavirus –Cart wheel
Coronavirus –petal shaped peplomers
Adenovirus –space vehicle shaped
Astrovirus ---Star shaped
B) Direct detection from specimens
For viruses that are difficult to cultivate ,EM can be used as primary tool for diagnosis
Faeces Rotavirus, Adenovirus
Norwalk like viruses
Astrovirus, Calicivirus
Vesicle Fluid HSV
VZV
Skin scrapings papillomavirus, orf
molluscum contagiosum
As an alternative to tissue culture
As tissues culture is time consuming and technically demanding ,EM is used as an alternative :-
1) Vesicular rashes –HSV and VZV detection from vesicular fluid
2) Meningitis—Detection of enterovirus and mumps from CSF.
Virus detection from tissue cultures EM can be used for detection of viral growth in tissue culture
The sensitivity and specificity of EM can be improved by adding specific antiviral antibody to the specimen to aggregate the virus particles which can be centrifuged
The sediment is negatively stained and viewed under EM
Direct immumofluroscence
The document discusses diseases of laboratory rats and control measures. It begins by introducing laboratory rats as an animal model and defining disease. It then outlines general preventive measures like procuring pathogen-free animals, using microisolation cages, and maintaining proper temperature, humidity and ventilation. Several common bacterial, fungal, viral and parasitic diseases are described in detail, including their symptoms, transmission, diagnosis and treatment. The document emphasizes preventive measures and controls to maintain healthy colonies and prevent research complications from infectious diseases.
Adenoviruses are medium sized, nonenveloped DNA viruses that commonly infect the respiratory, gastrointestinal, and urinary tracts of humans and animals. They cause a variety of clinical manifestations depending on the infected site, including respiratory infections, pneumonia, eye infections, and gastrointestinal illness. Adenoviruses are diagnosed through cell culture, antigen detection, molecular methods like PCR, and serology. Treatment is supportive and prevention relies on good hygiene practices and environmental disinfection. Some adenovirus types were previously used in vaccines but are no longer manufactured.
This document summarizes cryptococcal meningitis, a fungal infection of the membranes surrounding the brain and spinal cord that is common in people with HIV/AIDS. It describes the causative organism, Cryptococcus neoformans, and outlines the clinical presentation, diagnosis, and treatment of cryptococcal meningitis. The treatment involves amphotericin B and flucytosine initially, followed by long-term fluconazole therapy and antiretroviral treatment once the patient's CD4 count recovers. Prevention strategies include screening high-risk HIV patients and treating asymptomatic cryptococcal infections before starting antiretroviral therapy.
Coronaviruses & Rotaviruses. General Properties and Laboratory DiagnosisEneutron
Coronaviruses and rotaviruses are common causes of respiratory and gastrointestinal infections in humans. Coronaviruses are enveloped viruses that cause common colds and gastroenteritis, while rotaviruses are double-shelled viruses that appear wheel-shaped under microscopy and are a major cause of diarrhea in infants and children worldwide. Laboratory diagnosis of these infections can be done through electron microscopy of feces to detect viral morphology, serological testing of paired patient sera to detect rising antibody titers, and virus isolation, though this last method is not widely used for coronaviruses. Treatment is supportive for coronavirus infections as no antiviral drugs or vaccines exist, while rehydration therapy and rotavirus vaccines are
This document discusses various laboratory methods for diagnosing tuberculosis (TB), including:
- Sputum smear microscopy to detect acid-fast bacilli, the most common initial diagnostic method.
- Nucleic acid amplification tests like PCR and GeneXpert that can rapidly detect TB in sputum through DNA amplification.
- Culture-based methods grown on solid or liquid media to isolate Mycobacterium tuberculosis from clinical samples, which is then tested for drug susceptibility.
- Immunological tests like interferon-gamma release assays that detect TB infection by measuring T-cell responses to TB antigens.
It provides details on the principles, advantages, and limitations of different microbiological, molecular,
Recent outbreak of diseases related to humans.MedhaPathak2
Recently we have faced many outbreak of human diseases throughout the world, one of them being COVID-19. This presentation gives a detailed information about these outbreaks. Main focus is on COVID-19 as it is the prevailing outbreak.
Newer techniques in laboratory diagnosis of malariaAbhishek Singh
This document summarizes information about malaria, including:
- Malaria is caused by Plasmodium parasites transmitted via mosquito bites that infect and destroy red blood cells, causing symptoms like fever and fatigue.
- There are multiple Plasmodium species that cause malaria in humans. The life cycle involves the parasite reproducing in the liver and blood.
- Complications of malaria include organ enlargement, anemia, and other issues. Treatment involves antimalarial drugs with the goal of cure and prevention of transmission.
Parasitological diagnostic techniques by Negash alaminNegash Alamin
This document discusses methods for diagnosing parasitic pathogens. It outlines various diagnostic techniques including microscopy, molecular testing like PCR, antigen/antibody detection, radiology, and biopsy. For each major parasite group (protozoa, helminths), it describes common species and their appropriate diagnostic methods. Microscopy is commonly used but has limitations, while molecular tests like PCR provide increased accuracy even if more expensive. The goal is to match diagnosis techniques to specific parasites' characteristics for reliable identification.
This document discusses maxillofacial infection, including:
- The fascial spaces of the maxillofacial region and how infection can spread between them.
- Neurological complications of maxillofacial infection, especially cavernous sinus thrombosis which can cause issues like sensory deficits, eye movement problems, and increased intracranial pressure.
- Potentially fatal complications if the infection spreads to dangerous areas like the mediastinum or retroperitoneal spaces.
Herpes Viruses. General properties, Laboratory diagnostics.Eneutron
The document discusses herpesviruses, including their classification, properties, and laboratory diagnosis of associated human diseases. It describes the three subfamilies of herpesviruses - Alphaherpesvirinae, Betaherpesvirinae, and Gammaherpesvirinae - and provides examples such as herpes simplex virus types 1 and 2, varicella-zoster virus, cytomegalovirus, and Epstein-Barr virus. Methods for laboratory diagnosis of infections caused by these viruses are outlined, including virus isolation, serological techniques, and molecular methods. Rapid diagnosis may involve microscopy to detect intranuclear inclusion bodies. The document also covers epidemiology, pathogenesis, treatment and prophylaxis of major her
Lab diagnosis of Sexually transmitted Infections (STIs)Mostafa Mahmoud
This lecture was presented to the physicians dealing with the various infectious diseases specially in STIs in Riyadh Region, MOH. The lecture concentrates about the various methodology applied to diagnose STIs in the laboratory with the advantages and disadvantages of each. Hope to make benefits to all.
1. Curviform bacteria like Campylobacter, Helicobacter, and Vibrio can cause diseases in humans.
2. Campylobacter causes enterocolitis and diarrhea. Helicobacter pylori causes gastritis and peptic ulcers.
3. Spirochetes are spiral-shaped bacteria including Treponema, Borrelia, and Leptospira. Some are human pathogens that cause diseases like syphilis, Lyme disease, and relapsing fever.
Sputum examination provides important diagnostic information by analyzing material coughed up from the lungs and respiratory tract. Key indications for sputum examination include identifying the causative organism in suspected lower respiratory infections like pneumonia or tuberculosis. Sputum samples can also be examined cytologically to detect malignant cells or investigate other infections. Proper collection and transport of sputum samples is important for microbiological culture and other tests. Staining and microscopic examination of sputum looks for bacteria, fungi, parasites and other pathogenic organisms. Molecular tests like PCR provide a rapid and sensitive method for tuberculosis diagnosis.
Similar to Report of recent microbial techniques developed in diagnosing some common diseases (20)
Climate Impact of Software Testing at Nordic Testing DaysKari Kakkonen
My slides at Nordic Testing Days 6.6.2024
Climate impact / sustainability of software testing discussed on the talk. ICT and testing must carry their part of global responsibility to help with the climat warming. We can minimize the carbon footprint but we can also have a carbon handprint, a positive impact on the climate. Quality characteristics can be added with sustainability, and then measured continuously. Test environments can be used less, and in smaller scale and on demand. Test techniques can be used in optimizing or minimizing number of tests. Test automation can be used to speed up testing.
“An Outlook of the Ongoing and Future Relationship between Blockchain Technologies and Process-aware Information Systems.” Invited talk at the joint workshop on Blockchain for Information Systems (BC4IS) and Blockchain for Trusted Data Sharing (B4TDS), co-located with with the 36th International Conference on Advanced Information Systems Engineering (CAiSE), 3 June 2024, Limassol, Cyprus.
Generative AI Deep Dive: Advancing from Proof of Concept to ProductionAggregage
Join Maher Hanafi, VP of Engineering at Betterworks, in this new session where he'll share a practical framework to transform Gen AI prototypes into impactful products! He'll delve into the complexities of data collection and management, model selection and optimization, and ensuring security, scalability, and responsible use.
Sudheer Mechineni, Head of Application Frameworks, Standard Chartered Bank
Discover how Standard Chartered Bank harnessed the power of Neo4j to transform complex data access challenges into a dynamic, scalable graph database solution. This keynote will cover their journey from initial adoption to deploying a fully automated, enterprise-grade causal cluster, highlighting key strategies for modelling organisational changes and ensuring robust disaster recovery. Learn how these innovations have not only enhanced Standard Chartered Bank’s data infrastructure but also positioned them as pioneers in the banking sector’s adoption of graph technology.
GraphSummit Singapore | The Art of the Possible with Graph - Q2 2024Neo4j
Neha Bajwa, Vice President of Product Marketing, Neo4j
Join us as we explore breakthrough innovations enabled by interconnected data and AI. Discover firsthand how organizations use relationships in data to uncover contextual insights and solve our most pressing challenges – from optimizing supply chains, detecting fraud, and improving customer experiences to accelerating drug discoveries.
UiPath Test Automation using UiPath Test Suite series, part 6DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 6. In this session, we will cover Test Automation with generative AI and Open AI.
UiPath Test Automation with generative AI and Open AI webinar offers an in-depth exploration of leveraging cutting-edge technologies for test automation within the UiPath platform. Attendees will delve into the integration of generative AI, a test automation solution, with Open AI advanced natural language processing capabilities.
Throughout the session, participants will discover how this synergy empowers testers to automate repetitive tasks, enhance testing accuracy, and expedite the software testing life cycle. Topics covered include the seamless integration process, practical use cases, and the benefits of harnessing AI-driven automation for UiPath testing initiatives. By attending this webinar, testers, and automation professionals can gain valuable insights into harnessing the power of AI to optimize their test automation workflows within the UiPath ecosystem, ultimately driving efficiency and quality in software development processes.
What will you get from this session?
1. Insights into integrating generative AI.
2. Understanding how this integration enhances test automation within the UiPath platform
3. Practical demonstrations
4. Exploration of real-world use cases illustrating the benefits of AI-driven test automation for UiPath
Topics covered:
What is generative AI
Test Automation with generative AI and Open AI.
UiPath integration with generative AI
Speaker:
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
20 Comprehensive Checklist of Designing and Developing a WebsitePixlogix Infotech
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How to Get CNIC Information System with Paksim Ga.pptxdanishmna97
Pakdata Cf is a groundbreaking system designed to streamline and facilitate access to CNIC information. This innovative platform leverages advanced technology to provide users with efficient and secure access to their CNIC details.
UiPath Test Automation using UiPath Test Suite series, part 5DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 5. In this session, we will cover CI/CD with devops.
Topics covered:
CI/CD with in UiPath
End-to-end overview of CI/CD pipeline with Azure devops
Speaker:
Lyndsey Byblow, Test Suite Sales Engineer @ UiPath, Inc.
Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!SOFTTECHHUB
As the digital landscape continually evolves, operating systems play a critical role in shaping user experiences and productivity. The launch of Nitrux Linux 3.5.0 marks a significant milestone, offering a robust alternative to traditional systems such as Windows 11. This article delves into the essence of Nitrux Linux 3.5.0, exploring its unique features, advantages, and how it stands as a compelling choice for both casual users and tech enthusiasts.
Threats to mobile devices are more prevalent and increasing in scope and complexity. Users of mobile devices desire to take full advantage of the features
available on those devices, but many of the features provide convenience and capability but sacrifice security. This best practices guide outlines steps the users can take to better protect personal devices and information.
National Security Agency - NSA mobile device best practices
Report of recent microbial techniques developed in diagnosing some common diseases
1. REPORT OF RECENT MICROBIAL TECHNIQES DEVELOPED IN
DIAGNOSING SOME COMMON DISEASES
PRESENTING BY:
RATNAKAR
PHARM-D (2ND-YEAR)
SGRRITS, UTTARAKHAND
2. SOME COMMON DISEASE AND DIAGNOSIS
1.TYPHOID AND DIAGNOSIS
2.TUBERCULOSIS
3.MALARIA
4.CHOLERA
5.HEPATITIS
6.MENINGITITS
7.SYPHILIS
8.GONORRHEA
9.HIV/AIDS
3. TYPHOID CONTI.....
• Typhoid
Typhoid is a bacterial infection that can lead to a high fever, diarrhea, and
vomiting. It can be fatal. It is caused by the bacteria Salmonella typhi.
Causes
Bacterium Salmonella Typhi .
Salmonellae typhi
Salmonellae typhae are gram – ve rods, facultative aerobic, Motile
with peritrichate flagella, non-spore-forming
1-3μm ×0.5μm in size
DIAGNOSIS: according to week
First Week
Blood Culture, Bone Marrow Culture
4. Typhoid conti..
Second Week
Antibody Detection (Widal Test)
Widal Test is an agglutination test which detects the presence of serum
agglutinins (H and O) in patients serum with typhoid and paratyphoid fever.
" A test involving agglutination of typhoid bacilli when they are mixed with serum
containing typhoid antibodies from an individual having typhoid fever; used to
detect the presence of Salmonella typhi and S.paratyphi."
Third Week
Stool Culture
Fourth Week
Urine Culture
5. Typhoid conti...
NEW DIAGNOSTIC TESTS
• IDL Tubex detects IgM09 antibodies with in few minutes
• Typhidot test that detects presence of IgM and IgG in one hour
(sensitivity>95%, Specificity 75%)
• Typhidot-M, that detects IgM only (sensitivity 90% and specificity 93%)
• Typhidot rapid (sensitivity 85% and Specificity 99%) is a rapid 15 minute
immunochromatographic test to detect IgM.
2.Tuberculosis
Tuberculosis is an infectious disease that usually affects the lungs. Compared
with other diseases caused by a single infectious agent, tuberculosis is the
second biggest killer, globally.
6. Tuberculosis conti...
cause
Mycobacterium Tuberculosis Discoverer by Robert Koch so that disease is also
called koch disease.
Mycobacteria
• Obligate aerobes growing most successfully in tissues with a high oxygen
content, such as the lungs.
Diagnosis
Acid-fast staining
The material to be examined on a glass slide and staining with carbol-fuchsin
by means of either the Ziehl-Neelsen or Kinyoun technique.
Fluorochrome staining :
Sensitivity of detection of acid-fast organisms is increased by a fluorochrome
staining procedure with auramine O, a fluorescent stain.
7. Tuberculosis conti...
• This procedure requires use of a fluorescent microscope but is faster than
acid-fast staining because the intensity of the fluorescent signal enables
slides to be scanned at lower magnification.
Cultures
• Cultures of mycobacteria require only 10 to 100 organisms to detect M.
tuberculosis.
• The sensitivity of culture is excellent, ranging from 80% to 93%.
• The specificity is quite high, at 98%.
Radiometric and Colorimetric Detection Systems
• Radiometric culture systems (the BACTEC) incorporate 14 14C-labeled
palmitic acid into a liquid culture medium.
8. Tuberculosis conti...
• Growth of mycobacteria results in liberation of CO2 that can be measured by
the detection device.
• The increased sensitivity of the system enables growth to be detected
sooner, usually in 10 to 14 days.
Chromatography
• High-performance liquid chromatography (HPLC) is a rapid and highly
specific method for detecting the unique pattern of mycolic acids for
identifying mycobacterial species.
• Tuberculostearic acid, a component of M. tuberculosis, can be easily
detected even in infinitesimal (femtomole) quantities by gas liquid
chromatography (GLC).
Nucleic Acid Amplification Tests
• In each test, either DNA or ribonucleic acid (RNA) is amplified to detectable
levels.
9. Tuberculosis conti...
• Nucleic acid amplification tests include those that involve PCR amplification,
transcriptionmediated amplification, strand-displacement amplification, ligase
chain reaction, and Q Beta replicase amplification.
• NAA assays are also quite specific for M. tuberculosis, with specificities in the
range of 98% to 99%.
Polymerase chain reaction (PCR)
• PCR targets DNA, rRNA, insertion and repetitive elements, and various
proteinencoding genes.
• The PCR amplification process can be completed in 2–4 h after obtaining the
• processed clinical sample. The detection assay requires an additional 2–24 h
but it is very costly
10. Tuberculosis conti...
Transcription-mediated amplification (TMA) “MTD test”
Isothermal, target-based amplification system is based on amplification of
ribosomal RNA (rRNA) unlike PCR, which is based on amplification of DNA.
3.Malaria
Malaria is a life-threatening mosquito-borne blood disease caused by a
Plasmodium parasite.
It is transmitted to humans through the bite of the Anopheles mosquito.
Once an infected mosquito bites a human, the parasites multiply in the host's
liver before infecting and destroying red blood cells.
Diagnosis
• Light microscopy
11. Malaria conti...
• Antigen detection - Rapid malaria diagnosis tests (RDTs)
• Serology
• PCR
CULTURE OF THE PARASITE:
• Trager and Jensen’s method of petridish culture employed with a candle jar
to provide an atmosphere of 3% oxygen and 10% carbondioxide and a
relatively simple culture medium supplemented with human, rabbit or calf
serum to maintain infected erythrocytes. Fresh red cells were added
periodically for continuation and growth of Plasmodium.
• Rapid diagnostic tests (RDTs) are also employed for easy detection and take
around 2 to 15 minutes. These tests detect parasite antigens. These can be
used by relatively untrained staff.
12. Cholera conti..
4.Cholera
Cholera is an acute epidemic infectious disease. It is characterized by watery
diarrhea, extreme loss of fluid and electrolytes, and severe dehydration. It can be
fatal.
It is caused by the bacterium Vibrio cholera (V. cholera).
Diagnosis
• Rapid diagnosis of cholera by coagglutination test
A simple, rapid, and reliable method to detect Vibrio cholerae in fecal specimens
would assist in the management of cases of severe diarrhea, especially since
most such cases occur in areas with minimal laboratory facilities. A
coagglutination test was used to detect V. cholerae antigen in bile-peptone broth
incubated with feces.
• PCR test
• Bipolar microorganismus by bluedomethylene staining
13. HEPATITIS Conti..
VIRAL HEPATITIS
• The term viral hepatitis is used to describe infection of thenliver caused by
hepatotropic viruses. Currently there are 5 main varieties of these viruses
and a sixth poorlycharacterised virus, causing distinct types of viral hepatitis:
• Hepatitis A virus (HAV), causing a faecally-spread selflimiting disease.
• Hepatitis B virus (HBV), causing a parenterally transmitted disease that may
become chronic.
• Hepatitis C virus (HCV), previously termed non-A, non-B (NANB) hepatitis
virus involved chiefly in transfusionrelated hepatitis.
• Hepatitis delta virus (HDV) which is sometimes associated as superinfection
with hepatitis B infection.
• Hepatitis E virus (HEV), causing water-borne infection.
14. HEPATITIS Conti..
• Hepatitis G virus (HGV), is a recently discovered transfusion-transmitted
hepatotropic virus but is not known to cause hepatitis.
All these human hepatitis viruses are RNA viruses except HBV which is a DNA
virus.
Diagnosis
Liver fuction test
PCR test
ELISA
Detection HBs(ab)
6.Meningitis
Meningitis is an inflammation of the meninges. The meninges are the three
membranes that cover the brain and spinal cord. Meningitis can occur when
fluid surrounding the meninges becomes infected..
15. Meningitis conti..
The causative organisms
1. Escherichia coli infection is common in neonates with neural tube defects.
2. Haemophilus influenzae is commonly responsible for infection in infants and
children.
3. Neisseria meningitidis causes meningitis in adolescent and young adults and
is causative for epidemic meningitis.
4. Streptococcus pneumoniae is causative for infection at extremes of age and
following trauma.
Diagnosis
16. SYPHILIS Conti...
7.SYPHILIS
Syphilis is a venereal (sexually-transmitted) disease caused by spirochaetes,
Treponema pallidum. Other treponemal diseases are yaws, pinta and bejel. The
word ‘syphilis’ is
derived from the name of the mythological handsome boy,Syphilus, who was
cursed by Greek god Apollo with the disease.
Causative Organism
T. pallidum is a coiled spiral filament 10 μm long that moves actively in fresh
preparations. The organism cannot be stained by the usual methods and can
be demonstrated in the exudates and tissues by.
Diagnosis
1. Dark ground illumination (DGI) in fresh preparation;
2. Fluorescent antibody technique
17. SYPHILIS Conti...
3. silver impregnation techniques; and
4. PCR as a research method.
The organism has not been cultivated in any culture media but experimental
infection can be produced in rabbits and chimpanzees. The organism is rapidly
destroyed by cold, heat, and antiseptics.
8.Gonorrhea
Gonorrhea is a sexually transmitted disease (STD). It’s caused by infection with
the bacterium Neisseria gonorrhoeae.
Diagnosis
Microscopy of a direct smear of the discharge stained with Gram stain reveals
Gram-negative diplococci within polymorphonuclear leucocytes. The sensitivity
of the microscopy is highest in urethral samples in men, reaching 90–95%,
whereas for endocervical smears sensitivity drops to 30–50%. In asymptomatic
patients sensitivity is extremely low (20%).
18. Gonorrhea conti...
• Culture of N. gonorrhoeae requires the use of agar enriched with blood or
hemoglobin and several agents such as glucose, amino acids and antibiotics
(colistin, vancomycin, nystatin, etc.), to suppress the growth of commensal
neisseriae, and Gram-positive and Gram-negative bacteria and fungi.
• Enzyme immunoassays (EIAs) can be used for quick identification of
gonococcal urethritis with good sensitivity and specificity. These tests lack
sensitivity and specificity when used for cervical, pharyngeal and rectal
samples.
• Nucleic acid detection methods enable the detection of N. gonorrhoeae from
clinical samples without the requirement of bacterial viability. These are the
nucleic acid amplification tests (NAATs) that are used in pharyngeal and
rectal samples, with greater sensitivity than cultures.
19. HIV/ AIDS conti..
9.HIV/ AIDS
HIV is a virus that damages the immune system. The immune system helps the
body fight off infections. Untreated HIV infects and kills CD4 cells, which are a
type of immune cell called T cells.
Diagnosis
ELISA
An enzyme-linked immunosorbent assay, also called ELISA or EIA, is a test that
detects and measures antibodies in your blood. This test can be used to
determine if you have antibodies related to certain infectious conditions.
PROCEDURE
The ELISA test is simple and straightforward. You’ll probably need to sign a
consent form, and your doctor should explain the reason for doing the test.
The ELISA test involves taking a sample of your blood.
20. HIV/ AIDS conti..
• If your blood contains antibodies to the antigen, the two will bind together.
Western blot
• Western blot is often used in research to separate and identify proteins. In
this technique a mixture of proteins is separated based on molecular weight,
and thus by type, through gel electrophoresis.
The procedure involves:
• Separation of the proteins on polyacrylamide gel.
• Transfer (blotting) of proteins from the gel to a membrane (nitrocellulose or
nylon) and identification of the protein with a specific Ab.
• The method is sensitive for detecting proteins in complex mixtures.
• Immunoblot is used as a confirmatory test for HIV