DNA replication fidelity relies on processive DNA polymerases that remain stably bound to DNA during elongation. Processivity is conferred by DNA sliding clamps that bind polymerases, increasing replication speed. Additionally, fidelity is ensured by proofreading mechanisms where polymerases remove incorrectly paired bases using intrinsic exonuclease activity. Together, processive replication coupled with proofreading allows DNA to be accurately copied and passed to subsequent generations with high fidelity despite occasional mutations.

1. REPLICATION FIDELITY
Presented by
Mr.Karthick J.

2. INTRODUCTION :
• Fidelity of DNA replication is depend on the
enzyme to catalyze many reaction before
relasing its substrate is called processivity.
• The key of processivity of DNA polymerase tjat
act at the replication fork in their association
with protein called sliding DNA clamps.

3. • The speed of DNA synthesis is largely due to
the progressive nature of DNA polymerase III
due to its binds with sliding DNA clamps and
stabilizes itself in elongation process.
• Thus the replication fidelity increases.

5. • The another reason for fidelity is the proof
reading process of replication.
• In proof reading process, the incorrect base
pairs remove by the nucleus that originally
identified in the same polypeptide as the DNA
polymerase.
• In the polymerase 1 two nuclease , one is
exonuclease and one is endonuclease .
• Thus the fidelity is increased of the DNA
replication .

6. CONCLUSION :
• DNA replication itself can occasionally
damage, called mutation that introduces error,
hence the maintainance of the DNA
replication is needed for the next generation
purpose.
• DNA molecules are themselves irreplaceable,
it can cause cellular damage too.

7. • Fidelity of DNA replication depends on the
proof reading, repair system as well as in
some enzymes function.
• Such mechanism of DNA replication fidelity is
also depends on the onway repair system like
nick translation done by the phosphodiester
bonds and with the help of ATPs.

8. THANK YOU