Dentin forms in primary, secondary, and tertiary layers. Secondary dentin develops after root formation is complete and deposits more slowly with fewer, wavier tubules. Tertiary dentin forms in reaction to stimuli and has fewer, irregular tubules. Dentin is innervated by nerves that pass through tubules. Clinically, dentin permeability and adhesion of materials depends on its surface and tubule patency. Endodontic treatment success decreases with age as secondary and tertiary dentin decrease the pulp chamber and canal. Dentin tubule occlusion can reduce sensitivity, and mitochondrial DNA within tubules aids forensic identification.