Pulse Oximeter


         R. C. Gupta
         S. S. Ahluwalia
         CSIO, Chandigarh
Pulse Oximeter
   Pulse Oximeter is a medical electronic instrument that measures
    Oxygen Saturation (SaO2), the percentage of Hemoglobin saturated
    with Oxygen & Pulse Rate by Non Invasive technique

   In this the absorption of light by oxygenated and reduced
    hemoglobin is measured optically at two wavelengths (RED at 660
    nm & Infrared at 940 nm)

   It enables prompt recognition of Hypoxemia in patients

   This instrument is immensely useful in monitoring arterial
    oxygenation in paediatrics including Neonatal Units, OTs, Post-
    operative Recovery Wards, Trauma Wards. Oximetry has been
    recommended as a standard instrument for general anaesthetic

   Pulse Oximetry is also used in monitoring of Pulmonary diseases
    in adults & in investigation of sleep disorders
Pulse Oximeter

   Oxygen is transported in blood in two distinct states : under
    normal physiological conditions, about 98% of Oxygen in the blood
    is combined with Hb inside the RED blood cells. The remaining 2%
    is physically dissolved in the plasma

   The amount of O2 bound to Hb in a given quantity of arterial blood
    is usually represented by the percent SaO2 which is defined as the
    ratio of Oxy Hemoglobin (HbO2) concentration to the total
    concentration of Hb



                             HbO2
             SaO2 % = ---------------------- X 100
                       ( HbO2 + Hb )
Pulse Oximeter
                               Principle

     Oximetry works by trans-illuminating the tissue containing an
     arterial blood and evaluating absorption of RED & INFRARED light

i)   The light absorbance of oxygenated Hemoglobin (HbO2) is different
     from that of reduced Hemoglobin (Hb) at the Oximeter’s two
     wavelengths
Pulse Oximeter
                               Principle

ii)   The absorbance at both wavelengths have pulsatile (AC)
      component, which is the result of fluctuating volume of arterial
      blood between source and detector
Pulse Oximeter

The output light at each wavelength consists of two
components, referred to as AC & DC components respectively

Based on Beer’s law, Mendelson and Kent derived the relation
between SaO2 and R/IR ratio
                         ε IR(Hb) (R/IR) - ε R(Hb)
SaO2   =

           [ε IR(Hb) – εIR(HbO2)] (R/IR) + [ε R(HbO2) – εR(Hb)]

Where ε (Hb) & ε (HbO2) are millimolar extinction coefficients of
reduced & oxyhemoglobin respectively

R / IR = A 660 nm / A 940 nm = (ACR / DCR) / (ACIR / DCIR)
Pulse Oximeter

     ε IR(Hb) = 0.20     εR(Hb) = 0.86
     ε IR(HbO2) = 0.29   εR(HbO2) = 0.12




     Calibration Curve
1 V P-P
                           50 Hz

                          CONTROL
                           SIGNAL
                                                Pulse Oximeter
                         GENERATOR
                                                                                                       PULSE SHAPING
                                                                                       VACR               CIRCUIT

      LED
     DRIVERS

                                                                                                VACR
Red LED        IR LED                           RED CHANNEL
660nm           940 nm                                                           SEPARATOR




                                       SAMPLE
                                                                          RED




                                        HOLD
                                                    SUBTRACTOR   FILTER              OF                MULTIPLIER      ADC




                                         &
                                                                                   AC & DC
                                                                                COMPONENT
                                                                                                                               SaO2 AND
                                                                                                                              PULSE RATE
                                                                                                                                DISPLAY
  FINGER TIP

                                                 AMBIENT
                              I/V
                                       SAMPLE

                                        HOLD     LIGHT                                                                         µP with
                           CONVERTER
                                                 CHANNEL                                                               I/O
                                         &


                              &                                                                                                memories
   PHOTO
  DETECTOR                   PRE-
                                                                                        VDCR      VDCIR                PORT
                           AMPLIFIER


                                                                                                                              KEYBOARD

FINGER PROBE
                                                                                 SEPARATOR
     2500 S
                                                                                     OF
                                       SAMPLE

                                        HOLD




                                                    SUBTRACTOR   FILTER            AC & DC             MULTIPLIER
                                                                                                                       ADC
                                         &




                                                                          IR    COMPONENT
                                                IR CHANNEL                                    VACIR




                    RED LED ON

                    BOTH LEDs OFF

                    IR LED ON
                                                                 BLOCK DIAGRAM
                    BOTH LEDs OFF

                625 S
 TIMING SEQUENCE
Pulse Oximeter
SPECIFICATIONS :
Measurement Range:
 Oxygen Saturation (SaO2)        :       40-100%
 Pulse Rate (BPM)                :       30-240 bpm

Accuracy:
 Oxygen Saturation (SaO2)        :       ±2 Digits
 Pulse Rate (BPM)                :       ±3 Digits

Resolution:
 Oxygen Saturation (SaO2)        :       1%
 Pulse Rate (BPM)                :       1BPM

Display:
 Two Three digit red seven segment display for oxygen saturation and pulse rate.
 Ten segment bar graph display for pulse amplitude.

Sensor Compatibility:
 DS-100A                         :       Nellcor Dura sensor for Adult
 Wavelength                      :       660nm (RED), 940nm (IR)
Pulse Oximeter

SPECIFICATIONS :
Alarms   and Indication:
         Alarm for high and low SaO2 percentage.
         Alarm for high and low Pulse Rate.
         Beep sound with each detected pulse when a sensor’s derived signal is
          present.
         Visual indication if sensor accidentally slipped from finger.
         RS232 PC interface for data downloading and displaying Plethysmographic
          signal.
         Battery Low warning and alarms.

Trend facility:
        Store the trend data upto 100 patients and 24Hrs of run time.
        With Trend Analysis Software.
Electrical Specification:
         Mains Voltage           :                230V AC, 50Hz
         Power Consumption       :                Less than 1 watt
         Battery Backup          :                Inbuilt rechargeable Lead Acid
                                                  battery of 6V/3.2AH With 16 hrs
                                                  backup at full charge
Pulse Oximeter




Lab. & Clinical Trials


We have tested and calibrated the system by using BIO-
TEK SpO2 simulator in the whole range of SaO2 and
Pulse    Rate    and    then    taken  to   Deptt.   of
Anaesthesia, PGI, Chandigarh for its clinical trials on
patients. The results of the system were compared with
Datex Ohmeda (PGI) System and found satisfactory as
per our specifications.
Pulse Oximeter




Know-how transferred & Status :

The know-how of Pulse Oximeter was transferred to
M/s Pranavam Life Care Instruments (P) Ltd, Ernakulam & in
production
DIFFERENT AVAILABLE MODELS OF PULSE OXIMETER
 BCI



BIG SIZE                SMALL SIZE           FINGER TYPE
PRICE: US $1395.00      PRICE: US $1095.00   PRICE: $279.95
PRICE: RS 69750         PRICE: RS 54750      PRICE: RS 13975




                     HANDHELD SIZE
                     PRICE: US $ 695.00
                     PRICE: RS 34750
DIFFERENT AVAILABLE MODELS OF PULSE OXIMETER

NELLCOR




                BIG SIZE         HANDHELD SIZE
OHMEDA




 BIG SIZE   BIG SIZE + PRINTER   HANDHELD SIZE
Pulse Oximeter
Flow chart for calculation of SaO2

Pulse oximeter lecture

  • 1.
    Pulse Oximeter R. C. Gupta S. S. Ahluwalia CSIO, Chandigarh
  • 2.
    Pulse Oximeter  Pulse Oximeter is a medical electronic instrument that measures Oxygen Saturation (SaO2), the percentage of Hemoglobin saturated with Oxygen & Pulse Rate by Non Invasive technique  In this the absorption of light by oxygenated and reduced hemoglobin is measured optically at two wavelengths (RED at 660 nm & Infrared at 940 nm)  It enables prompt recognition of Hypoxemia in patients  This instrument is immensely useful in monitoring arterial oxygenation in paediatrics including Neonatal Units, OTs, Post- operative Recovery Wards, Trauma Wards. Oximetry has been recommended as a standard instrument for general anaesthetic  Pulse Oximetry is also used in monitoring of Pulmonary diseases in adults & in investigation of sleep disorders
  • 3.
    Pulse Oximeter  Oxygen is transported in blood in two distinct states : under normal physiological conditions, about 98% of Oxygen in the blood is combined with Hb inside the RED blood cells. The remaining 2% is physically dissolved in the plasma  The amount of O2 bound to Hb in a given quantity of arterial blood is usually represented by the percent SaO2 which is defined as the ratio of Oxy Hemoglobin (HbO2) concentration to the total concentration of Hb HbO2 SaO2 % = ---------------------- X 100 ( HbO2 + Hb )
  • 4.
    Pulse Oximeter Principle Oximetry works by trans-illuminating the tissue containing an arterial blood and evaluating absorption of RED & INFRARED light i) The light absorbance of oxygenated Hemoglobin (HbO2) is different from that of reduced Hemoglobin (Hb) at the Oximeter’s two wavelengths
  • 5.
    Pulse Oximeter Principle ii) The absorbance at both wavelengths have pulsatile (AC) component, which is the result of fluctuating volume of arterial blood between source and detector
  • 6.
    Pulse Oximeter The outputlight at each wavelength consists of two components, referred to as AC & DC components respectively Based on Beer’s law, Mendelson and Kent derived the relation between SaO2 and R/IR ratio ε IR(Hb) (R/IR) - ε R(Hb) SaO2 = [ε IR(Hb) – εIR(HbO2)] (R/IR) + [ε R(HbO2) – εR(Hb)] Where ε (Hb) & ε (HbO2) are millimolar extinction coefficients of reduced & oxyhemoglobin respectively R / IR = A 660 nm / A 940 nm = (ACR / DCR) / (ACIR / DCIR)
  • 7.
    Pulse Oximeter ε IR(Hb) = 0.20 εR(Hb) = 0.86 ε IR(HbO2) = 0.29 εR(HbO2) = 0.12 Calibration Curve
  • 8.
    1 V P-P 50 Hz CONTROL SIGNAL Pulse Oximeter GENERATOR PULSE SHAPING VACR CIRCUIT LED DRIVERS VACR Red LED IR LED RED CHANNEL 660nm 940 nm SEPARATOR SAMPLE RED HOLD SUBTRACTOR FILTER OF MULTIPLIER ADC & AC & DC COMPONENT SaO2 AND PULSE RATE DISPLAY FINGER TIP AMBIENT I/V SAMPLE HOLD LIGHT µP with CONVERTER CHANNEL I/O & & memories PHOTO DETECTOR PRE- VDCR VDCIR PORT AMPLIFIER KEYBOARD FINGER PROBE SEPARATOR 2500 S OF SAMPLE HOLD SUBTRACTOR FILTER AC & DC MULTIPLIER ADC & IR COMPONENT IR CHANNEL VACIR RED LED ON BOTH LEDs OFF IR LED ON BLOCK DIAGRAM BOTH LEDs OFF 625 S TIMING SEQUENCE
  • 9.
    Pulse Oximeter SPECIFICATIONS : MeasurementRange: Oxygen Saturation (SaO2) : 40-100% Pulse Rate (BPM) : 30-240 bpm Accuracy: Oxygen Saturation (SaO2) : ±2 Digits Pulse Rate (BPM) : ±3 Digits Resolution: Oxygen Saturation (SaO2) : 1% Pulse Rate (BPM) : 1BPM Display: Two Three digit red seven segment display for oxygen saturation and pulse rate. Ten segment bar graph display for pulse amplitude. Sensor Compatibility: DS-100A : Nellcor Dura sensor for Adult Wavelength : 660nm (RED), 940nm (IR)
  • 10.
    Pulse Oximeter SPECIFICATIONS : Alarms and Indication:  Alarm for high and low SaO2 percentage.  Alarm for high and low Pulse Rate.  Beep sound with each detected pulse when a sensor’s derived signal is present.  Visual indication if sensor accidentally slipped from finger.  RS232 PC interface for data downloading and displaying Plethysmographic signal.  Battery Low warning and alarms. Trend facility: Store the trend data upto 100 patients and 24Hrs of run time. With Trend Analysis Software. Electrical Specification: Mains Voltage : 230V AC, 50Hz Power Consumption : Less than 1 watt Battery Backup : Inbuilt rechargeable Lead Acid battery of 6V/3.2AH With 16 hrs backup at full charge
  • 11.
    Pulse Oximeter Lab. &Clinical Trials We have tested and calibrated the system by using BIO- TEK SpO2 simulator in the whole range of SaO2 and Pulse Rate and then taken to Deptt. of Anaesthesia, PGI, Chandigarh for its clinical trials on patients. The results of the system were compared with Datex Ohmeda (PGI) System and found satisfactory as per our specifications.
  • 15.
    Pulse Oximeter Know-how transferred& Status : The know-how of Pulse Oximeter was transferred to M/s Pranavam Life Care Instruments (P) Ltd, Ernakulam & in production
  • 16.
    DIFFERENT AVAILABLE MODELSOF PULSE OXIMETER BCI BIG SIZE SMALL SIZE FINGER TYPE PRICE: US $1395.00 PRICE: US $1095.00 PRICE: $279.95 PRICE: RS 69750 PRICE: RS 54750 PRICE: RS 13975 HANDHELD SIZE PRICE: US $ 695.00 PRICE: RS 34750
  • 17.
    DIFFERENT AVAILABLE MODELSOF PULSE OXIMETER NELLCOR BIG SIZE HANDHELD SIZE OHMEDA BIG SIZE BIG SIZE + PRINTER HANDHELD SIZE
  • 19.
    Pulse Oximeter Flow chartfor calculation of SaO2