Protein arrays can be created using several methods, including robotic spotting, inkjet printing, and photolithography to array proteins onto a solid surface like a microscope slide. The proteins are immobilized using coatings like hydrophilic polymers or by chemical treatments with amines, aldehydes or epoxies. There are different types of protein arrays that can be created for various applications such as disease biomarker discovery or drug target identification.

1. PROTEIN ARRAYS
1
By
KAUSHAL KUMAR SAHU
Assistant Professor (Ad Hoc)
Department of Biotechnology
Govt. Digvijay Autonomous P. G. College
Raj-Nandgaon ( C. G. )

2. Synopsis
• Introduction
• Preparation of protein arrays
• Types of protein arrays
• Applications
• References
2

3. 3

4. Preparation of protein microarray
Different methods of arraying the proteins :
1. Robotic method
2. Ink jetting method
3. Piezoelectric method
4. Photolithography
The proteins are arrayed onto a solid surface such
as microscope slides, membranes, beads or
microtitre plates. 4

5. Immobilising agents include layers of aluminium or
gold, hydrophilic polymers, and polyacrylamide
gels, or treatment with amines, aldehyde or epoxy.
Thin-film technologies like physical vapour
deposition (PVD) and chemical vapour deposition
(CVD) are employed to apply the coating to the
support surface.
5

6. 6
Types of protein arrays

7. 7

8. 8

9. 9

10. 10

11. 11

12. 12

13. 13

14. References
• Principle of Gene manipulation & Genomics :by
Primrose & twymane:2nd edition ,2004
• Proteomics by Timothy Palzkill,2001 ,1st edition
• National center for biotechnology center
14