This document provides an overview of shotgun DNA mapping using single molecule DNA analysis techniques. It discusses digesting genomic DNA into random fragments, constructing DNA tethers for optical tweezers unzipping, simulating unzipping profiles to create a library for comparison to experimental data, and potential applications for studying chromatin structure, transcription, and telomeres. The goal is to map DNA sequences to their genomic locations in order to better understand protein-DNA interactions and gene expression.