The document discusses a study on DNA unzipping using optical tweezers and explores the role of proteins in this process. It details the methods for detecting sequence changes and bound proteins, as well as a shotgun DNA mapping algorithm for analyzing unzipped fragments. Experimental data is referenced, particularly in relation to the yeast genome.

1. A Tale of Two LabsUnzipping DNAandMolecular Motors

2. Microtubules

3. Kinesin

4. Slide preparation

5. Osmolytes

6. Part 2DNA Unzipping

7. Unzipping DNAComponents:Unzipping constructOptical TweezersDNA to unzipOptional: proteins

8. We can unzip anythingFFFor plot below:Red Line – protein bound to DNABlack Line – naked DNABlack Dotted Line- predictions of protein locationsArrows – missing protein locationsUnzipping can detect:Naked DNA “sequence”Changes to DNA sequenceproteins bound to DNAAnd other possible structures

9. Shotgun DNA MappingAlgorithm matches unzipped random digestion fragments to library of simulated unzipping fragmentsInitial data is based on yeast genomeLibrary of Simulated CurvesExperimental ForceCorrect Match